(12) United States Patent
`Carter et al.
`
`US006407213B1
`US 6,407,213 B1
`(io) Patent No.:
`(45) Date of Patent:
`Jun. 18, 2002
`
`(54) METHOD FOR MAKING HUMANIZED
`ANTIBODIES
`
`(75)
`
`Inventors: Paul J. Carter; Leonard G. Presta,
`both of San Francisco, CA (US)
`
`(73) Assignee: Genentech, Inc., South San Francisco,
`CA (US)
`
`( * ) Notice:
`
`Subject to any disclaimer, the term of this
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`08/146,206
`Jun. 15, 1992
`PCT/US92/05126
`
`(21) Appl. No.:
`(22) PCT Filed:
`(86) PCT No.:
`§ 371 (c)(1),
`(2), (4) Date: Nov. 17, 1993
`
`Related U.S. Application Data
`
`(63) Continuation-in-part of application No. 07/715,272, filed on
`Jun. 14, 1991, now abandoned.
`
`(51) Int. CI.7
`(52) U.S. CI.
`
`C07K 16/00
`530/387.3; 435/69.6; 435/69.7;
`435/70.21; 435/91; 536/23.53; 424/133.1
`435/69.6, 69.7,
`(58) Field of Search
`435/70.21, 91, 172.2, 240.1, 240.27, 252.3,
`320.1, 328; 536/23.53; 424/133.1; 530/387.3
`
`(56)
`
`AU
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`
`530/388.22
`530/387.3
`. 530/389.3
`530/387.3
`424/133.1
`424/133.1
`.. 435/7.23
`530/387.2
`.. 435/69.6
`424/130.1
`530/387.3
`... 530/399
`530/387.3
`
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`
`Primary Examiner—Anthony C. Caputa
`Assistant Examiner—Minh-Tam Davis
`(74) Attorney, Agent, or Firm—Wendy M. Lee
`ABSTRACT
`(57)
`
`Variant immunoglobulins, particularly humanized antibody
`polypeptides are provided, along with methods for their
`preparation and use. Consensus immunoglobulin sequences
`and structural models are also provided.
`
`82 Claims, 9 Drawing Sheets
`
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