`Cabilly et al.
`
`111111
`
`1111111111111111111111111111111111111111111111111111111111111
`US006331415Bl
`US 6,331,415 B1
`Dec. 18, 2001
`
`(10) Patent No.:
`(45) Date of Patent:
`
`(54) METHODS OF PRODUCING
`IMMUNOGLOBULINS, VECTORS AND
`TRANSFORMED HOST CELLS FOR USE
`THEREIN
`
`(75)
`
`Inventors: Shmuel Cabilly, Monrovia; Herbert L.
`Heyneker, Burlingame; William E.
`Holmes, Pacifica; Arthur D. Riggs, La
`Verne; Ronald B. Wetzel, San
`Francisco, all of CA (US)
`
`(73) Assignee: Genentech, Inc., South San Francisco,
`CA(US)
`
`( *) Notice:
`
`Subject to any disclaimer, the term of this
`patent is extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`
`(21) Appl. No.: 07/205,419
`
`(22) Filed:
`
`Jun. 10, 1988
`
`Related U.S. Application Data
`
`( 63) Continuation of application No. 06/483,457, filed on Apr. 8,
`1983, now Pat. No. 4,816,567.
`
`(51)
`
`Int. Cl? ........................... C12N 15/13; C12N 15/00;
`C12N 15/63
`
`(52) U.S. Cl. ....................... 435/69.6; 435/69.1; 435/69.7;
`435/70.1; 435/70.21; 435/71.1; 435/71.2;
`435/320; 435/252.1; 435/252.3; 435/252.33;
`435/254.11; 435/254.2; 435/254.21; 435/455;
`435/471; 435/483; 435/485
`
`(58) Field of Search .................................. 435/69.1, 69.7,
`435/71.1, 70.1, 71.2, 320, 261, 252.1, 252.3,
`81, 55, 56, 69.6, 252.33, 254.21, 483
`
`(56)
`
`References Cited
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`Primary Examiner-Phillip Gambel
`(74) Attorney, Agent, or Firm-Bums, Doane, Swecker &
`Mathis, LLP
`
`(57)
`
`ABSTRACT
`
`The invention relates to processes for producing an immu(cid:173)
`noglobulin or an immunologically functional immunoglo(cid:173)
`bulin fragment containing at least the variable domains of
`the immunoglobulin heavy and light chains. The processes
`can use one or more vectors which produce both the heavy
`and light chains or fragments thereof in a single cell. The
`invention also relates to the vectors used to produce the
`immunoglobulin or fragment, and to cells transformed with
`the vectors.
`
`36 Claims, 19 Drawing Sheets
`
`Merck Ex. 1001, pg 1
`
`
`
`US 6,331,415 B1
`Page 2
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