Poly-L-lysine solution
`
`Catalog Number P8920
`Store at Room Temperature
`
`TECHNICAL BULLETIN
`
`CAS RN 25988-63-0
`
`Product Description
`The loss of paraffin and frozen sections from slides has
`long been a problem during routine histologic staining
`procedures. Various adhesives including albumin,
`gelatin, and chrome alum have been applied to slides to
`minimize this loss.1-3 Different solutions of poly-L-lysine
`have been shown to be most effective in promoting
`adhesion of sections.4,5 The polycationic nature of this
`molecule allows interaction with the anionic sites of
`tissue sections resulting in strong adhesive properties.5
`Poly-L-lysine has been demonstrated as an effective
`tissue adhesive for use in various microwave
`procedures.
`
`This product contains 0.1% (w/v) poly-L-lysine in
`deionized water with 0.01% thimerosal, added as a
`preservative.
`
`Poly-L-lysine Solution is for use in adhering tissue
`sections to glass slides, particularly useful with
`immunohistochemical techniques. It is intended for use
`as an adhesive subbing solution for immunohisto-
`chemistry (IHC) and routine histologic staining
`preparations. For uses other than IHC or routine
`histology, the end user must determine if this product is
`suitable for the intended use.
`
`Equipment Required but Not Provided.
`• Microscope slides
`• Slide rack
`• Plastic containers and graduated cylinder
`• Drying oven (optional)
`Precautions and Disclaimer
`This product is for R&D use only, not for drug,
`household, or other uses. Please consult the Material
`Safety Data Sheet for information regarding hazards
`and safe handling practices.
`
`Preparation Instructions
`Dilute Poly-L-lysine Solution 1:10 with deionized water
`prior to coating slides. Use plastic containers and
`graduated cylinders when mixing or storing solution,
`and coating slides. Do not add fresh solution to used
`diluted working solution.
`
`Storage/Stability
`Store the product at room temperature. It is stable until
`expiration date shown on label.
`
`Store the diluted working solution in refrigerator at
`2–8 (cid:176) C. The diluted working solution is stable for at
`least three months. Filter diluted solution after use.
`
`Discard solutions if turbidity or bacterial growth
`develops.
`
`Procedure
`Notes: Slides must be clean before attempting this
`procedure. Clean with acidic alcohol (i.e., 1% HCl in
`70% ethanol) if necessary.
`
`When diluted according to instructions, the maximum
`number of slides that can be coated is 900 per liter of
`diluted working solution. Exceeding 900 slides per liter
`will affect the performance of the product.
`
`Do not add fresh solution to used diluted solution.
`
`1. Allow diluted working Poly-L-lysine Solution to
`come to room temperature (18–26 (cid:176) C) before use.
`2. Place clean slides, a rack at a time, in diluted
`working Poly-L-lysine Solution for 5 minutes.
`Increasing incubation time does not improve
`performance.
`
`3. Drain slides and dry in 60 (cid:176) C oven for 1 hour or at
`room temperature (18–26 (cid:176) C) overnight.
`Results
`Solution promotes adhesion of paraffin and frozen
`sections to slides for routine histologic procedures.
`
`Page 1 of 2
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`HOLOGIC EXHIBIT 1033
`Hologic v. Enzo
`
`

`
`References
`1. Culling, C.F.A. et al., Cellular Pathology Technique,
`4th ed. Butterworth & Co. Ltd. (Boston, MA: 1985)
`p 98.
`2. Theory and Practice of Histological Techniques,
`Bancroft, J.D., and Stevens, A., eds, Churchill
`Livingstone (New York, NY: 1982) pp 75-76.
`3. Manual of Histologic Staining Methods of the
`Armed Forces Institute of Pathology, 3rd ed., Luna,
`L.G., ed., McGraw-Hill (New York, NY: 1968) p 28.
`
`4. Mazia, D. et al., Adhesion of cells to surfaces
`coated with poly-L-lysine. J Cell Biol., 66,198, 1975.
`5. Huang, W.M. et al., Improved section adhesion for
`immunocytochemistry using high molecular weight
`polymers of L-lysine as a slide coating. Histochem.,
`77, 275, 1983
`
`KAA,MAM 10/12-1
`
`(cid:211) 2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other
`countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their
`particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at
`www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.
`
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