`
`Last updated: October 6, 1999
`
`Order info
`Gold Seal #3010
`Shandon Lipshaw #121 (800-245-6212) <= Each rack holds 30 slides
`Shandon Lipshaw #121
`<= Each chamber holds 350 mL
`
`Qty
`Materials
`Glass microscope slides 60
`Slide rack
`2
`Slide chamber
`6
`ddH2O
`~5 L
`NaOH
`70 g
`95% Ethanol
`420 mL
`Poly-L-lysine
`70 mL Sigma #P 8920
`Tissue culture PBS
`70 mL
`Vacuum oven (45C)
`Slide box (plastic only) 1
`
`VWR #48443-806
`
`1. Place slides in slide racks. Place racks in chambers.
`2. Prepare CLEANING SOLUTION:
`Dissolve 70 g NaOH in 280 mL ddH2O.
`Add 420 mL 95% ethanol. Total volume is 700 mL (= 2 X 350 mL); stir until completely mixed.
`If solution remains cloudy, add ddH2O until clear.
`3. Pour solution into chambers with slides; cover chambers with glass lids. Mix on orbital shaker for 2 hr.
`Once slides are clean, they should be exposed to air as little as possible. Dust particles will interfere with coating and
`printing.
`4. Quickly transfer racks to fresh chambers filled with ddH2O. Rinse vigorously by plunging racks up and down.
`Repeat rinses 4X with fresh ddH2O each time. It is critical to remove all traces of NaOH-ethanol.
`5. Prepare POLYLYSINE SOLUTION:
`70 mL poly-L-lysine + 70 mL tissue culture PBS in 560 mL water.
`Use plastic graduated cylinder and beaker.
`6. Transfer slides to polylysine solution and shake 15 min. - 1 hr.
`7. Transfer rack to fresh chambers filled with ddH2O. Plunge up and down 5X to rinse.
`8. Centrifuge slides on microtiter plate carriers (place paper towels below rack to absorb liquid) for 5 min. @ 500 rpm.
`Transfer slide racks to empty chambers with covers for transport to vacuum oven.
`9. Dry slide racks in 45C vacuum oven for 10 min. (Vacuum is optional.)
`10. Store slides in closed slide box (plastic only, without rubber mat bottom)
`11. BEFORE PRINTING ARRAYS:
`Check that polylysine coating is not opaque.
`Test print, hyb and scan sample slides to determine slide batch quality.
`
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`HOLOGIC EXHIBIT 1032
`Hologic v. Enzo