NOV. 8.2005
`
`4:5lPM
`
`ENZO BIOCHEM
`
`No_715o
`
`p_
`
`1
`
`RECEIVED
`CENTRAL FAX CENTE
`NOV 0 s 2005
`
`'£ESP0lIISE UNDER 37 6.F.R, 51, HQ
`EXPEDITED PROCEDURE
`
`Ji—“’1Afl1’-i—~‘’‘‘’ " ’“"’
`
`
`Attorney Docket No.: Enz-7(P)lC3I
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`In re Application of:
`
`Jannis Stavrianopoulos et al.
`
`Serial No.:
`
`08/486,070
`
`Group Art Unit: 1631
`
`Filed:
`
`For:
`
`June 7, 1995
`
`Primary Exam'r: John S. Brusca
`
`NUCLEIC ACIDS FIXED OR IMMOBILIZED TO NON-POROUS
`SOLID SUPPORT. AND SYSTEM, ARRAY AND NON-POROUS
`GLASS OR PLASTIC SOLID SUPPORT COMPRISING SUCH FIXED
`OR IMMOBILIZED NUCLEIC ACIDS
`
`Confirmation No.:
`
`CERTIFICATE OF FA CSIMILE TRANSMISSION
`
`Commissioner for Patents
`P.O. Box 1450
`
`Alexandria, VA 22313-1450
`
`Sir:
`
`I hereby certify that the ttached correspondence (total of 52 paes
`
`including this sheet) comprising:
`
`Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.Fl. §1.116
`(including Exhibits A, B & C)
`
`was sent to the United States Patent and Trademark Office by telefax to (571)
`
`273-8300.
`
`bmitted, R spectfully
` Date:’ NovemberI8, 2005
`
`
`
`onald C. Fedus, Reg. No. 32,567
`Natalie Bogdanos, Reg. No. 51,480
`Attorneys for Applicants
`Enzo Life Sciences, Inc.
`
`clo Enzo Biochem, Inc.
`527 Mdison Avenue, 9th Floor
`New York, NY 10022-4304
`Telephone (212) 583-0100
`Facsimile (212) 583-0150
`
`Enz-7IPIIC3I
`
`PAGE 1l52 * RCVD AT 11l8I2005 4:50:40 PM [Eastern standard Time] ’ SVRIIISPTO-EFXRF-6127 * DNIS:2738300 ’ CS|D:212583015fl ’ DURATION (mm-ss)'10-12
`
`Page 1 of 52
`
`HOLOGIC EXHIBIT 1024
`
`Hologic V. Enzo
`
`Page 1 of 52
`
`HOLOGIC EXHIBIT 1024
`Hologic v. Enzo
`
`

`
`NOV. 8.2005
`
`4:52PM
`
`ENZO BIOCHEM
`
`NQ7160
`
`p_
`
`2
`
`RECEIVED
`CENTRAL FAX CENTE
`Nov 0 8 2005
`
`Fggsgq/Its: UNDER 37 C.F.R. § 1, 1 ;§
`EXPEDE§D PROCEDURE
`anoup ART umr 1631
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`
`
`Applicantlsl:
`
`Stavrianopoulos, et al.
`
`Serial No.:
`
`08/486,070
`
`Filed:
`
`June 7, 1995
`
`For:
`
`NUCLEIC ACIDS FIXED on IMMOBILIZED
`T0 NON-POROUS soLID SUPPORT. AND
`SYSTEM. ARRAY AND NON-POROUS GLASS )
`on PLASTIC soLID SUPPORT COMPRISING )
`SUCH FDCED on lMMOBlLIZED NUCLEIC AcIDs )
`)
`
`Group Art Unit: 1631
`
`Primary Examiner: John S. Brusca
`
`527 Madison Avenue, 9?“ Floor
`New York, NY 10022-4304
`November 8. 2006
`
`l l ) l l ) l ) l
`
`VIA FILED BY FA QSIMILE TRANSMISSI_Qfl
`TO: /703} 872-9306
`
`Mail Stop -5 Non-Fee Amendment
`Commissioner for Patents
`
`A
`
`P.O. Box 1450
`Alexandria, VA 22313-1 450
`
`SQPPLEMENTAL AMENDMENT TO APPLICANTS‘ NOVEMBER 2, 2005
`AMENDMENT UNDER 37 C.F.R. §1.116
`
`Dear-Sirs: -
`
`This paper (Supplemental Amendment) follows Applicants‘ November 2,
`2005 Amendment Under 37 C.F.R. §1.116, the latter having been filed in response
`to the Offlce Action mailed on September 29, 2005 in connection with the above-
`identified application. Their November 2, 2005 Amendment Under 37 C.F.R.
`51.116 was timely filed. This paper (Supplemental Amendment) is also being
`
`timely filed with no extension request or fee being due.
`
`Enz-7(P)(C3l
`
`PAGE 2l52 ’ RCVD AT 11l3l2005 4:50:40 PM [Eastern standard Time] ‘ SVRIUSPTO-EFXRF-6127 * DNlS:273330lJ ' CSlD:212583ll150 ' DURATION (mm-ss)'10-12
`
`Page 2 of 52
`
`Page 2 of 52
`
`

`
`NOV.
`
`8. 2005
`
`4:52PM
`
`ENZO BIOCHEM
`
`NQ7160
`
`p_
`
`3
`
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 2 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`
`
`
`
`
`
`I hereby certify that this paper (Supplemental
`Amendment To Applicants‘ November 2. 2005
`Amendment Under 37 c.F.R. §1 .1 16 (along with any
`documents referred to as attached or enclosed) is being
`facsimile transmitted to the United States Patent and
`Trademarks (Fax No. 571-273-8300) on November 8.
`2005.
`Nov? Z005 N
`Date
`Ronald C. Fedus
`Reg. No. 32.567
`
`
`CERTIFICATE OF TRANSMISSION
`
`
`
`
`
`
`
`Amendments to the Specification and a New Abstract Of The Disclosure are
`
`requested as reflected in Exhibits A and C accompanying this paper.
`
`Remarks begin on page 42 of this paper.
`
`Enz-7(P)(C3)
`
`PAGE 3l52 ‘ RCVD AT 11l8l2005 4:50:40 PM [Eastern standard Time] ' SVR:USPTO-EFXRF-612? ' DNlS:2m300 ' CSlD:2125830150 ' DURATION (mm-ss):10-12
`
`Page 3 of 52
`
`Page 3 of 52
`
`

`
`NOV.
`
`8. 2005
`4:52PM
`ENZO BIOCHEM
`N0_7]50
`p_
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 3 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. {i1 .116]
`— November 8, 2005]
`
`4
`
`ueyy Title of the Invention:
`
`Please change the title to:
`-- SYSTEM, ARRAY AND NON-POROUS SOLID SUPPORT COMPRISING
`
`FIXED OR IMMOBILIZED NUCLEIC ACIDS -- .
`
`Amendments :9 the Specification:
`4
`Replace page 1 of the specification with new Page 1 provided as Exhibit A to this
`paper.‘ A marked—up version of Page 1 is also provided as Exhibit A.
`
`New Abstract Of The Disclosure:
`Replace the former Abstract with the new Abstract Of The Disclosure provided as
`
`Exhibit C to this paper.
`
`Amendments to the Claims: This listing of the claims below will replace all prior
`versions and listing of claims In this application?
`
`Listing of Claims:
`
`Claims 1-3143 (Canceled).
`
`:._??._—_—:——:Z.
`‘ An identical claim for priority was made in Applicants‘ March 7, 2001
`Amendment Under 37 C.F.R. §1 .115 (see page 2 of that paper, copy provided as
`Exhibit B).
`2 No claims are being amended, canceled or added by this paper.
`
`Enz-7(Pl(C3)
`
`PAGE 4l52 * RCVD AT 11l8I20D5 4:50:40 PM [Eastem Standard Time] ‘ SVR:USPTO~EFXRF-612? ’ DNlS:2?33300 ' CS|D'212583U150 ‘ DURATION (mm-ss)'10 12
`
`Page 4 of 52
`
`Page 4 of 52
`
`

`
`NOV. 8.2005
`
`4:52PM
`
`ENZO BIOCHEM
`
`NQ7150
`
`p_
`
`5
`
`Stavrianopoulos et aI., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 4 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116l
`-— November 8, 2005]
`
`Claim 3144.
`
`(Previously Presented) A non-porous solid support comprising one or
`
`more amine(s), hydroxylisl or epoxlde(s) thereon, wherein at least one single-
`
`stranded nucleic acid is fixed or immobilized in hybridizable form to said non-porous
`
`solid support via said one or more amine(s), hydroxyl(s) or epoxidels).
`
`Claim 3145.
`
`(Previously Presented) A non-porous solid support comprising one or
`
`more amine(s), hydroxyI(s) or epoxide(s) thereon, wherein at least one double-
`
`stranded nucleic acid is fixed or immobilized to said non-porous solid support via
`
`said one or more aminelsl, hydroxyl(s) or epoxide(s). and wherein one nucleic acid
`
`strand of said at least one double-stranded nucleic acid has covalently attached
`
`thereto either:
`
`(i)
`
`(ii)
`
`a non-radioactive signaling moiety, or
`
`a non-radioactive bridging moiety covalently or non-covalently
`
`attached to a non-radioactive signaling moiety.
`
`Claim 3146.
`(Previously Presented) The non-porous solid support of claims 3144,
`3145, 3287, 3324 or 3325, wherein said non-porous solid support comprises glass
`
`or plastic.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`Claim 3147.
`3145, 3287, 3324 or 3325, wherein said non-porous solid support comprises a Z
`4
`plate or plates, a well or wells, a microtiter well or microtiter wells, a depression or
`
`depressions, a tube or tubes, or a cuvette or cuvettes.
`
`Claims 3148-3149.
`
`(Canceled).
`
`Claim 3150.
`
`(Previously Presented) The non-porous solid support of claim 3287 or
`
`3325, comprising one or more amines.
`
`Enz-7lP)(C3)
`
`PAGE 5).’)? * RCVD AT 11l8l2005 4:50:40 PM [Eastern standard Time] ‘ SVRIUSPTO-EFXRF-6127 ' DNlS:2738300 ' CSlD:2125830150 ' DURATION (mm-ss):10-12
`
`Page 5 of 52
`
`Page 5 of 52
`
`

`
`NOV.
`
`8. 2005
`
`4:52PM
`
`ENZO BIOCHEM
`
`NQ7150
`
`p_
`
`6
`
`Stavrlanopouios et ai., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 5 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`Claim 3151.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`
`3145, 3287, 3324 or 3325, wherein said non-porous solid support has been
`
`treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3152.
`
`(Previously Presented) The non-porous solid support of claim 31 51,
`
`wherein said surface treatment agent comprises an amine providing compound, an
`
`epoxy iue or solution, an acid solution, or ammonium acetate.
`
`Claim 3153.
`
`(Previously Presented) The non-porous solid support of claim 3151,
`
`wherein said blocking agent comprises Denhardt's solution.
`
`Claim 3154.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`
`3145, 3287, 3324 or 3325, wherein said fixation or immobilization to said non-
`
`porous solid support is covalent.
`
`Claim 3155.
`(Previously Presented) The non-porous solid support of claims 3144.
`3145, 3287, 3324 or 3325, wherein said fixation or immobilization to said non-
`
`porous solid support is non-covalent.
`
`1 Claim 3156. A(Canceied).P
`
`(Previously Presented) The non-porous solid support of claims 3145,
`Claim 3157.
`3287 or 3325, wherein one strand of said at least one double-stranded nucleic acid
`is fixed or immobilized to said non-porous solid support and the other strand of said
`at least one double-stranded nucleic acid is hybridized to said one strand.
`
`Enz-7(P)(C3)
`
`PAGE 6152 " RCVD AT 11l8l20li5 4:50:40 PM [Eastern standard Time] ' SVRIUSPTO-EFXRF-612? * DN|S:273830ll ' CS|D:2i25830150 ' DURAHON (mm-ss)'10-12
`
`Page 6 of 52
`
`Page 6 of 52
`
`

`
`NOV.
`
`8. 2005
`4:52PM
`ENZO BIOCHEM
`NQ7160
`P.
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 6 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`7
`
`Claim 3158.
`
`(Previously Presented) The non-porous solid support of claims 3144
`
`or 3324, wherein said nuclelc acid is DNA.
`
`Claim 3159.
`
`(Previously Presented) The non-porous solid support of claims 3145,
`
`3287 or 3325, wherein said nucleic acid is DNA.
`
`(Previously Presented) The non-porous solid_support of claim 3144,
`Claim 3160.
`wherein said nucleic acid comprises a nucleic acid sequence complementary to a
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`(Previously Presented) The non-porous solid support of claims 3145
`Claim 3161.
`or 3287, wherein one strand of said at least one double—stranded nucleic acid
`comprises a nucleic acid sequence of interest sought to be identified, quantified or
`
`sequenced.
`
`(Previously Presented) The non-porous solid support of claims 3144
`' Claim 3162.
`or 3324, wherein said single-stranded nucleic acid is unlabeled.
`
`(Previously Presented) The non-porous solid support of claims 3144,
`Claim 3163.
`3145, 3287, 3324 or 3325, wherein said non-porous solid support is transparent
`
`or translucent.
`
`(Previously Presented) The non-porous solid support of claims 3145
`Claim 3164,
`or 3287, wherein said non-radioactive signaling moiety is quantifiable in or from a
`fluid or solution, said quantity being proportional to the amount or quantity of said
`
`non-radioactive signaling moiety.
`
`Enz-7(P)(C3l
`
`PAGE 7l52 * RCVD AT 11l3l2005 4:50:40 PM [Eastern standard Time] ' SVR:USPTOwEFXRF-612? * nms:2maoo ‘ CSlD:2125830150 ’ DURA'|10N (mm/ss):10-12
`
`Page 7 of 52
`
`Page 7 of 52
`
`

`
`NOV.
`
`8. 2005
`4:53PM
`ENZO BIOCHEM
`N0.7l60
`P.
`Stavrianopouios et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1996)
`Page 7 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`8
`
`(Previously Presented) The non—porous solid support of claims 3145
`Claim 3165.
`or 3287, wherein said non-porous solid support is transparent or translucent, and
`said non-radioactive signalin moiety is quantifiable in or from a fluid or solution or
`from said non-porous solid support, said quantity being proportional to the amount
`
`or quantity of said non-radioactive signaling moiety.
`
`(Previously Presented) The non-porous solid support of claims 3145,
`Claim 3166.
`3287 or 3325, wherein said non-radioactive signaling moiety comprises a
`
`chromagen or a chromagenic compound.
`
`(Previously Presented) The non-porous solid support of claims 3145,
`Claim 3167.
`3287 or 3325, wherein a non-radioactive signal from said non-radioactive signaling
`moiety is quantifiable or detectable by photometric techniques.
`
`(Previously Presented) The non-porous solid support of claims 3144
`Claim 3168.
`or 3324, comprising more than one single-stranded nucleic acid.
`
`(Previously Presented) The non—porous solid support of claims 3145.
`Claim 3169.
`3287 or 3325, comprising more than one double-stranded nucleic acid.
`
`Claims 3170-3171 (Canceled).
`
`(Previously Presented) A system comprising a non-porous solid
`Claim 3172.
`support which comprises one or more aminels), hydroxyi(s) or epoxide(s) thereon,
`wherein at least one single-stranded nucleic acid is fixed or immobilized thereto in
`hybridizabie form to said non-porous solid support via said one or more aminels),
`hydroxyllsl or epoxideisl.
`
`Enz-7(P)(C3)
`
`"‘°”’5“°V°“T1W?°°54=5°=4°Pll [Eastern SlfiildaldTime]'SVR:USPl0-EFXRF-6127*DN|S:2i38300'CS|D:2l2583ll15U'DURA110N(mm/ss)'1l-12
`
`Page 8 of 52
`
`Page 8 of 52
`
`

`
`NOV.
`
`8. 2005
`4:53PM
`ENZO BIOCHEM
`N0.7l60
`P.
`Stavrianopouios et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 8 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 20051
`
`9
`
`(Previously Presented) A system comprising a non—porous solid
`Claim 3173.
`support which comprises one or more amine(s), hydroxyl(s) or epoxidelsl thereon,
`wherein at least one double-stranded nucleic acid is fixed or immobilized to said
`non—porous solid support via said one or more amine(s), hydroxyilsl or epoxide(s),
`and wherein one nucleic acid strand of said double-stranded nucleic acid has
`
`covalently attached thereto either:
`(i)
`a non-radioactive signaling moiety, or
`(ii)
`a non-radioactivebridging moiety covalently or non-covalently
`attached to a non-radioactive signaling moiety.
`
`(Previously Presented) The system of claims 3172. 3173, 3279,
`Claim 3174.
`3280, 3281 or 3282, wherein said non—porous solid support comprises glass or
`
`plastic.
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3175.
`3280, 3281 or 3282, wherein said non—porous solid support comprises a pi
`plates, a well or wells, a microtiter well or microtiter wells, a depression or
`depressions, a tube or tubes, or a cuvette or cuvettes.
`
`Claims 3176-3178.
`
`(Canceled).
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3179.
`3280, 3281 or 3282, wherein said non-porous solid support has been treated with
`a surface treatment agent, a blocking agent, or both.
`
`(Previously Presented) The system of claim 3179. wherein said
`Claim 3180.
`n epoxy glue or
`surface treatment agent comprises an amine providing compound, a
`
`solution, an acid solution or ammonium acetate.
`
`Enz-7(P)(C3)
`
`PAGE 9l52 ' RCVD AT i1l8l2005 4:50:40 PM [Eastern Standard Time] ‘ SVR:USPTO-EFXRF-612? ’ DN|S:2?3830li' CSlD:2125830150‘ DURA'|10N (mm-ss)'10-12
`
`Page 9 of 52
`
`Page 9 of 52
`
`

`
`NOV.
`
`.
`.
`82005
`4.53PM
`ENZOBIOCHEM
`Nam,
`P.
`Stavrlanopoulos et ai., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 9 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116l
`-- November 8, 2005]
`
`10
`
`Claim 3181.
`
`(Previously Presented) The system of claim 3179, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3182.
`3280, 3281 or 3282, wherein said fixation or immobilization to said non-porous
`
`solid support is covalent.
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3183.
`3280, 3281 or 3282, wherein said fixation or immobilization to said non-porous
`
`solid support is non—covalent.
`
`Claim 3184.
`
`(Canceled).
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`Claim 3185.
`wherein one strand of said doubie—stranded nucleic acid is fixed or immoblllzed to
`said non-porous solid support and the other nucleic acid strand of said double-
`stranded nucleic acid is hybridized to said one strand.
`
`(Previously Presented) The system of claims 3172 or 3280, wherein
`Claim 3186.
`said nucleic acid is DNA.
`
`Claim 3187.
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`
`wherein said nucleic acid is DNA.
`
`(Previously Presented) The system of claim 3172, wherein said
`Claim 3188.
`nucleic acid comprises a nucleic acid sequence complementary to a nucleic acid
`sequence of interest sought to be identified, quantified or sequenced.
`
`Enz-'7(P)(C3i
`
`PAGE 105? ' RCVD AT 11i8i2UD54:50:-10 Pill [Eastern Standard Time] * SVRIUSPTO-EXRF-6l27 ‘ DNlS:2?383U0 * CS|D:2125830150 ' DURATION (mm-ss)'10-12
`
`Page 10 of 52
`
`Page 10 of 52
`
`

`
`NOV.
`
`8. 2005
`4:53PM
`ENZO BIOCHEM
`NQ7160
`P_ H
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 10 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116l
`-- November 8. 2005]
`
`(Previously Presented) The system of claims 3173 or 3288, wherein
`Claim 3189.
`one strand of said at least one double-stranded nucleic acid comprises a nucleic
`acid sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3190.
`
`(Previously Presented) The system of claim 3172, wherein said
`
`single-stranded nucleic acid is unlabeled.
`
`(Previously Presented) The system of claims 3172, 3173, 3279,
`Claim 3191.
`3280, 3281 or 3282, wherein said non-porous solid support is transparent or
`
`translucent.
`
`(Previously Presented) The system of claims 3173 or 3288, wherein
`Claim 3192.
`said non-radioactive signaling moiety is quantifiable in or from a fluid or solution,
`
`signaling moiety.
`
`(Previously Presented) The system of claims 3173 or 3288. wherein
`Claim 3193.
`said non-porous solid support is transparent or translucent, and said non-radioactive
`signaling moiety is quantifiable in or from a fluid or solution or from said non-porous
`M solid support, said quantityibeinig proportional to the amount or quantity of said
`non-radioactive signaling moiety.
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`Claim 3194.
`wherein said non-radioactive signaling moiety comprises a chromagen or a
`
`chromagenic compound.
`
`Enz-7(P)(C3)
`
`PAGE 115? ‘ RCVD AT 11l8l2U05 4:50:40 PM [Eastem standard Time)’ SVRIUSPTO-EXRF-6l2? ' DN|S:2?383ii0‘ CSlD:2125830150 * DURATION (mm-ss)'10-12
`
`Page 11 of 52
`
`Page 11 of 52
`
`

`
`NOV.
`
`8. 2005
`4:54PM
`ENZO BIOCHEM
`NQ7160
`P_
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (l-"lied June 7, 1995)
`Page 11 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`12
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`Claim 3195.
`wherein a non-radioactive signal from said non-radioactive signaling moiety is
`
`quantifiable or detectable by photometric techniques.
`
`Claim 3196.
`
`(Previously Presented) The system of claim 3172, comprising more
`
`than one single-stranded nucleic acid.
`
`Claim 3197.
`
`(Previously Presented) The system of claims 3173, 3281 or 3282,
`
`comprising more than one double—stranded nucleic acid.
`
`(Previously Presented) An array comprising various singie—stranded
`Claim 3198.
`nucleic acids fixed or immobilized in hybridizable form to a non-porous solid
`
`support.
`
`(Previously Presented) An array comprising various double-stranded
`Claim 3199.
`nucleic acids fixed or immobilized to a non-porous solid support, wherein at least
`one nucleic acid strand of said various double—stranded nucleic acids comprises at
`least one non-radioactive signaling moiety which is quantifiable or detectable.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3200.
`3326*or‘3327, wherein said non-porous solid support c'omprise's glassor plastic‘.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3201.
`3326 or 3327, wherein said non-porous solid support comprises a plate or plates, a
`well or wells, a microtiter well or microtiter wells, a depression or depressions, a
`
`tube or tubes, or a cuvette or cuvettes.
`
`Claims 3202-3203.
`
`(Canceled).
`
`Enz-7(P)(C3)
`
`PAGE12l52‘
`
`,
`,
`,
`.
`.
`.
`-
`--
`RCVDAT11llll20054.5ll.40Plll liastemstandardTime]*SVR.USPTO-EXRF-l)l2? DNlS.2?383l)0 cs|n.212ssao15o DURATION (mm-Ss):10-12
`
`Page 12 of 52
`
`Page 12 of 52
`
`

`
`NOV.
`
`3.
`:
`454PM
`2005
`ENZO BIOCHEM
`N0.-H60
`P.
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 12 [Supplemental Amendment To Applicants‘ November 2. 2005
`Amendment Under 37 C.F.R. §1.1'l6]
`-- November 8, 2005]
`
`13
`
`(Previously Presented) The array of claims 31 98, 3199, 3300, 3301,
`Claim 3204.
`3326 3327, comprising one or more amines.
`
`(Previously Presented) The array of
`Claim 3205.
`3326 or 3327, wherein said non-porous solid support has been treated w
`surface treatment agent, a blocking agent, or both.
`A
`
`ith a
`
`claims 3198, 3199, 3300, 3301,
`
`(Previously Presented) The array of claim 3205, wherein said surface
`Claim 3206.
`treatment agent comprises an amine providing compound, an epoxy glue or
`solution, an acid solution or ammonium acetate.
`
`(Previously Presented) The array of claim 3205, wherein said
`Claim 3207.
`blocking agent comprises Denhardt's solution.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3208.
`3326 or 3327, wherein said fixation or immobilization to said non-porous solid
`
`support is covalent.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3209.
`33-26 or 3327, wherein said fixation or immobilization to said non-porous solid’
`
`support is non-covalent.
`
`Claim 3210.
`
`(Canceled).
`
`Enz-7(P)(C3)
`
`PAGE 1352 ' RCVD AT 1li8i2005 4:50:40 PM [Easlem standard Time] ' SVRIUSPTO-EFXRF-5l27 " DN|S:27383ll0 ’ CSlD:212583lll50* DURATION (mm-ss)'10-12
`
`Page 13 of 52
`
`Page 13 of 52
`
`

`
`NOV.
`
`454PM
`2005
`ENZO BIOCHEM
`NQ7160
`P.
`3.
`:
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 13 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`14
`
`(Previously Presented) The array of claims 3199 or 3301. wherein
`Claim 3211.
`the first strands of said double-stranded nucleic acids are fixed or immobilized to
`said non-porous solid support and the other nucleic acid strands of said double-
`stranded nucleic acids are hybridized to said first strands.
`
`Claim 3212.
`
`(Previously Presented) The array of claims 3198 or 3300, wherein
`
`said nucleic acids is DNA.
`
`Claim 3213.
`
`(Previously Presented) The array of claims 3199 or 3301, wherein
`
`said nucleic acids is DNA.
`
`(Previously Presented) The array of claims 3198 or 3289, wherein
`Claim 3214.
`said nucleic acids comprise nucleic acid sequences complementary to nucleic acid
`sequences of interest sought to be identified, quantified or sequenced.
`
`(Previously Presented) The array of claims 3199 or 3290, wherein
`Claim 3215.
`one strand of said double-stranded nucleic acids comprises a nucleic acid sequence
`of interest sought to be identified, quantified or sequenced.
`
`(Previously Presented) The array of claims 3198 or 3300, wherein
`Claim 3216.
`said single-stranded nucleic acids are unlabeled.
`
`(Previously Presented) The array of claims 3198, 3199, 3300, 3301,
`Claim 3217.
`3326 or 3327, wherein said non-porous solid support is transparent or
`
`translucent.
`
`(Previously Presented) The array of claims 3199 or 3290, wherein
`Claim 3218.
`said non-radioactive signaling moiety is quantifiable in or from a fluid or solution,
`said quantity being proportional to the amount or quantity of said label or labels.
`
`Enz-7(P)(C3l
`
`PAGE 145?‘ RCVD AT 11l8l2fl05 4:50:40 PM [Eastern standard Time] ’ SVRIUSPTO-El‘-XRF-5l27 * DNlS:2738300 * CSlD:2125830150 ‘ DURATION (mm-ss)'10-12
`
`Page 14 of 52
`
`Page 14 of 52
`
`

`
`NOV.
`
`.
`-
`82005
`4.54PM
`ENZOBIOCHEM
`N0.7l60
`P.
`Stavrlanopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 14 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`15
`
`(Previously Presented) The array of claims 3199 or 3290, wherein
`Claim 3219.
`said non-porous solid support is transparent or translucent, and said non-radioactive
`signaling moiety is quantifiable in or from a fluid or solution or from said non-porous
`solid support, said quantity being proportional to the amount or quantity of said
`
`non-radioactive signaling moiety.
`
`(Previously Presented) The array of claims 3199, 3301, 3326 or
`Clalm 3220.
`3327, wherein said non-radioactive signaling moiety comprises a chromagen or a
`
`chromagenlc compound.
`
`(Previously Presented) The array of claims 3199, 3301 , 3326 or
`Claim 3221.
`3327, wherein a non-radioactive signal from said non-radioactive signaling moiety
`is quantifiable or detectable by photometric techniques.
`
`(Previously Presented) An array comprising various slngle-stranded
`Clalm 3222.
`nucleic acids fixed or immobilized in hybridizable fonn to a non-porous solid support
`
`having wells or depressions.
`
`(Previously Presented) An array comprising various double-stranded
`Claim 3223.
`nucleic acids tlxed or immoblllzedzto a non-porous solid support having wells or
`depressions, wherein at least one nucleic acid strand of each of said various
`double-stranded nucleic acids comprises at least one non-radioactive signaling
`moiety which is quantifiable or detectable.
`
`(Previously Presented) The array of claims 3222 or 3223, wherein
`Claim 3224.
`said non-porous solid support comprises glass or plastic.
`
`Enz-7(P)(C3)
`
`PAGE 155? ' RCVD AT 1ll8l2005 4:50:40 PM
`
`.
`-
`'
`I
`[Eas em standard Time] ’ SVRUSPTOEXRF-5l2? ' DNlS.2738300' CS|D:212583D150 * DURATION (mm-ss):10-12
`
`Page 15 of 52
`
`Page 15 of 52
`
`

`
`NOV.
`
`8.
`:
`454PM
`2005
`ENZO BIOCHEM
`Nolnéo
`P.
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7. 1995)
`Page 15 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`16
`
`Claims 3225-3226.
`
`(Canceled).
`
`(Previously Presented) The array of claims 3222 or 3223, wherein
`Claim 3227.
`said non-porous solid support comprises one or more amines.
`
`(Previously Presented) The array of claims 3222 or 3223, wherein
`Claim 3228.
`said non-porous solid support has been treated with a surface treatment agent, a
`
`blocking agent, or both.
`
`(Previously Presented) The array of claim 3228, wherein said surface
`Claim 3229.
`treatment agent comprises an amine providing compound, an epoxy glue or
`solution, an acid solution or ammonium acetate.
`
`(Previously Presented) The array of claim 3228, wherein said
`Claim 3230.
`blocking agent comprises Denhardt's solution.
`
`(Previously Presented) The array of claims 3222 or 3223, wherein
`Claim 3231.
`said fixation or immobilization to said non-porous solid support is covalent.
`
`(Previously Presented) The array of claims 3222 or 3223. wherein
`Claim 3232.
`said fixation or immobilization to said non-porous solid supportis non-covalent.
`
`Claim 3233.
`
`(Canceled).
`
`(Previously Presented) The array of claim 3223, wherein the first
`Claim 3234.
`of said double-stranded nucleic acids are fixed or immobilized to said non-
`strands
`double-stranded
`porous solid support and the other nucleic acid strands of said
`nucleic acids are hybridized to said first strands.
`
`Enz-7(Pl(C3)
`
`PAGE 16l52 ' RCVD AT 11l8l2005 4:50:40 PM [Eastem Standard Time] ' SVRIUSPTO-EXRF-5l2? ‘ DNlS:2738300 ' CSlD:2125830l50 * DURATION (mm-ss):10-12
`
`Page 16 of 52
`
`Page 16 of 52
`
`

`
`NOV.
`
`.
`;
`82005
`454PM
`ENZOBIOCHEM
`N0.7160
`P.
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`Page 16 [Supplemental Amendment To Applicants‘ November 2. 2005
`Amendment Under 37 C.F.R. §1.116l
`-- November 8, 2005]
`
`17
`
`Claim 3235.
`
`(Previously Presented) The array of claim 3222, wherein said nucleic
`
`acids are DNA.
`
`Claim 3236.
`
`(Previously Presented) The array of claim 3223, wherein said nucleic
`
`acids are DNA.
`
`(Previously Presented) The array of claim 3222, wherein said nucleic
`Clalm 3237.
`acids comprise a gene sequence or pathogen sequence.
`
`(Previously Presented) The array of claim 3223, wherein one strand
`Claim 3238.
`of said double-stranded nucleic acids comprises a gene sequence or pathogen
`
`sequence.
`
`Claim 3239.
`
`(Canceled).
`
`(Previously Presented) The array of claim 3222, wherein said single-
`Claim 3240.
`stranded nucleic acids are unlabeled.
`
`(Previously Presented) The array of claims 3222 or 3223, wherein
`Claim 3241.
`‘ said non-porous solid support is transparent or translucent.
`‘
`
`Claim 3242.
`
`eviously Presented) The array of claim 3223, wherein said non-
`(Pr
`said
`gnaling moiety is quantifiable in or from a fluid or solution,
`radioactive si
`radioactive
`quantity being proportional to the amount or quantity of said non-
`
`signaling moiety.
`
`Enz-7(P)(C3)
`
`PAGE 1752' RCVD AT 11l3I2005 4:50:40 PM [Eastern Standard Tlme]*SVR:USPTO-EXRF-5127* DNlS22738300 ‘ CSlD!2125830150 ’ DURATION (mm-ss)'10-12
`
`Page 17 of 52
`
`Page 17 of 52
`
`

`
`NOV.
`
`4: 55PM
`ENZO BIOCHEM
`N0.7l60
`P.
`8. 2005
`Stavrianopoulos et aI.. U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7. 1995)
`Page 17 [Supplemental Amendment To Applicants‘ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`18
`
`(Previously Presented) The array of claim 3223, wherein said non-
`Claim 3243.
`porous solid support is transparent or translucent, and said non-radioactive
`signaling moiety is quantifiable in or from a fluid or solution or from said non-porous
`solid support, said quantity being proportional to the amount or quantity of said
`non-radioactive signaling moiety.
`
`(Previously Presented) The array of claim 3223, wherein said non-
`Claim 3244.
`radioctive signaling moiety comprises a chromagen or a chromagenic compound.
`
`(Previously Presented) The array of claim 3223, wherein a non-
`Claim 3245.
`radioactive signal from said non-radioactive signaling moiety is quantifiable or
`
`detectable by photometric techniques.
`
`sup
`
`(Previously Presented) A non-porous glass or non-porous plastic solid
`Claim 3246.
`port comprising one or more aminelsl. hydroxyl(s) or epoxide(s) thereon,
`-stranded nucleic acid is fixed or immobilized in
`wherein at least one single
`aid one or more aminelsi.
`hybridizable form to said non-porous solid support via s
`
`hydroxyl(s) or epoxide(s).
`
`(Previously Presented) A non-porous glass or non-porous plastic solid
`Claim 3247.
`support comfprisingone or more aminels), hydroxyl(s) or epoxide(s) thereon,
`wherein at least one double—stranded nucleic acid is fixed or immobilized to said
`-porous glass or non-porous plastic solid support via said one or more amine(s),
`non
`and wherein one nucleic acid strand of said double-
`hydroxyllsi or epoxide(s),
`stranded nucleic acid has covalently attached thereto either:
`(i)
`a non-radioactive sinaling moiety, or
`(ii)
`a non-radioactive bridging moiety covalently or non-covalently
`attached to a non-radioactive signaling moiety.
`
`Enz-7 (P)(C3l
`
`PAGE 18l52‘RCVD AT 11l8l2005 4:50:40 PM
`
`I
`[Eas em
`
`Stan
`
`.
`-
`dard Time] " SVR.USPTO-EFXRF-6l27 ‘ DNlS.2738300 ‘ CS|D:2125830150 ' DURATION (mm-ss):10-12
`
`Page 18 of 52
`
`Page 18 of 52
`
`

`
`NOV.
`
`8. 2005
`
`4:55PM
`
`ENZO BIOCHEM
`
`Stavrianopoulos et al., U.S. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995).
`Page 18 [Supplemental Amendment To Applicants’ November 2, 2005
`Amendment Under 37 C.F.R. §1.116]
`-- November 8, 2005]
`
`No 7160
`
`P
`
`19
`
`(Previously Presented) The non—porous glass or non-porous plastic
`Claim 3248.
`non-porous
`soli
`:1 support of claims 3246 or 3247, wherein said non—porous glass or
`a well or wells, a microtiter well or
`plastic solid support comprises a plate or plates,
`microtiter wells, a depression or depressions, a tube or tubes, or a cuvette or
`
`cuvettes.
`
`Claims 3249-3251 .
`
`(Canceled) .
`
`Claim 3252.
`
`(Previously Presented) The non-porous glass or non-porous plastic
`lms 3246 or 3247, wherein said non—porous glass or non-porous
`solid support of cla
`coking
`plastic solid support has been treated with a surface treatment agent, a bi
`
`agent, or both.
`
`(Previously Presented) The non-porous glass or non-porous plastic
`Claim 3253.
`solid support of claim 325 2, wherein said surface treatment agent comprises an
`amine providing compound, an epoxy glue or solution, an acid solution or
`
`ammonium acetate.
`
`(Previously Presented) The non-porous glass or non-porous plastic
`Claim 3254.
`solid support or ciai'm'3252, whereln"said’biocking agent comprises Denhardt‘s
`
`solution.
`
`(Previously Presented) The non-porous glass or non-porous plastic
`Claim 3255.
`on or immobilization to
`solid suppori: of claims 3246 or 3247, wherein said fixati
`port is covalent.
`said non-porous glass or non-porous plastic solid sup
`
`Enz-7(P)(C3)
`
`-
`-
`] SVR.UsPi’0-EXRF-sill'DNis'2i38300'CsiD'2125830150*DURATION
`PAGE 19i52 * RCVD AT 11i8i2U05 4'5iJ'40 PM [Eastern standard Time '
`-
`-
`-
`
`(mmss):10-12
`
`Page 19 of 52
`
`Page 19 of 52
`
`

`
`NOV.
`
`3.
`:
`4 55PM
`2005
`ENZO BIOCHEM
`N