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`•
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`RESPONSE UNDER 37 C.F.R. §1.116
`EXPEDITED PROCEDURE
`EXAMINING GROUP ART UNIT 1631
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
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`Applicant(s) :
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`Stavrianopoulos, et al.
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`Serial No.:
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`08/486,070
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`Group Art Unit: 1631
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`Filed:
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`For:
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`June 7, 1995
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`Ex'r: Ardin H. Marschel, Ph.D.
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`/
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`ARRAYS AND SYSTEMS COMPRISING
`ARRAYS FOR GENETIC ANALYSES AND
`OTHER APPLICATIONS
`(As Previously Amended)
`
`527 Madison Avenue, 9th Floor
`New York, NY 1 0022~4304
`June 30,2004
`
`VIA EXPRESS MAIL
`
`Mail Stop Amendment
`Commissioner for Patents
`P.O. Box 1450
`Alexandria, VA 2231 3-1450
`
`AMENDMENT UNDER 37 C.F.R. §1.116
`(IN RESPONSE TO THE APRIL 7, 2004 OFFICE ACTION)
`
`Dear Sirs:
`
`This is a response (Amendment Under 37 C.F.R. §1.116) to the Office Action
`
`mailed on April 7, 2004 in connection with the above-identified application. A
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`response to the April 7, 2004 Office Action is initially due by July 7, 2004.
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`Accordingly, this response (Amendment Under 37 C.F.R. §1 .116) is being timely filed
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`and no extension request or fees are believed due.
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`Enz-7(P)(C3)
`
`Exhibit 2020 Page 1
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`Enzo Exhibit 2020
`Hologic, Inc. v. Enzo Life Sciences, Inc.
`Case IPR2016-00820
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`
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`5tavrianopoulos et al..5. Pat. Appl. 5er. No. 08/486,071iled June 7, 1995)
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`Page 2 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
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`EXPRESS MAIL CERTIFICATE
`"Express Mail" Label No. EV531083255US
`June 30. 2004
`Deposit Date
`I hereby certify that this paper and the attachments herein
`are being deposited with the United States Postal Service
`"Express Mail Post Office to Addressee" service under 37
`CFR 1.10 on the date indicated above and is addressed to
`~~p;3~i.:iO~ of Patents and Trademarks, Washington
`
`/W4~ JVNe. 3 0 2oo~
`Ronald C. Fedus
`Date
`Reg. No. 32,567
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 2
`
`
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`Stavrianopoulos et al..s. Pat. Appl. Ser. No. 08/486,07tliled June 7, 1995)
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`Page 3 [Amendment Under 37 C.F.R. §1 .116 -- June 30, 2004]
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`KINDLY AMEND THE ABOVE·IDENTIFIED APPLICATION AS FOLLOWS:
`
`In The Title of the Invention: Change the title to:
`
`-- Nucleic Acids Fixed or Immobilized to Non-Porous Solid Support, and
`
`System, Array and Non·Porous Glass or Plastic Solid Support Comprising
`
`Such Fixed or Immobilized Nucleic Acids --
`
`In The Claims: Please enter the complete listing of all claims provided below.
`
`Claims 1-2160 (Previously Canceled).
`
`Claims 2161-3143 (Canceled).
`
`Claim 3144. (New) A non-porous solid support comprising at least one single(cid:173)
`
`stranded nucleic acid directly or indirectly fixed or immobilized thereto in hybridizable
`
`form, wherein when said nucleic acid is indirectly fixed or immobilized to said non(cid:173)
`
`porous solid support, said indirect fixation or immobilization is not to a cell fixed in
`
`situ to said non-porous solid support.
`
`Claim 3145. (New) A non-porous solid support comprising at least one double(cid:173)
`
`stranded nucleic acid fixed or immobilized thereto, wherein at least one nucleic acid
`
`strand of said double-stranded nucleic acid comprises at least one non-radioactive
`
`chemical label which is quantifiable or detectable, wherein when said nucleic acid is
`
`indirectly fixed or immobilized to said non-porous solid support, said indirect fixation
`
`or immobilization is not to a cell fixed in situ to said non-porous solid support.
`
`Enz·7(P)(C3}
`
`Exhibit 2020 Page 3
`
`
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`5tavrianopoulos et al..5. Pat. Appl. 5er. No. 08/486,071=iled June 7, 1995)
`
`Page 4 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
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`Claim 3146. (New) The non-porous solid support of claim 3144 or 3145, wherein
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`said non-porous solid support comprises glass or plastic.
`
`Claim 3147. (New) The non-porous solid support of claim 3144 or 3145, wherein
`
`said non-porous solid support comprises a plate, a well or wells, a microtiter well or
`
`microtiter wells, depressions, tubes, cuvettes, beads, or a set of said plates, wells,
`
`depressions, tubes, cuvettes or beads.
`
`Claim 3148. (New) The non-porous solid support of claim 3144 or 3145, wherein
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`said non-porous solid support comprises more than one surface.
`
`Claim 3149. (New) The non-porous solid support of claim 31 44 or 3145, comprising
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`reactive sites or binding sites thereon, wherein said nucleic acid is fixed or
`
`immobilized to one of said reactive sites or binding sites.
`
`Claim 3150. (New) The non-porous solid support of claim 3149, wherein said
`
`reactive sites or binding sites comprise one or more amines, hydroxyls or epoxides.
`
`Claim 3151. (New) The non-porous solid support of claim 3144 or 3145, wherein
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`said non-porous solid support has been treated with a surface treatment agent, a
`
`blocking agent, or both.
`
`Claim 3152. (New) The non-porous solid support of claim 3151, wherein said
`
`surface treatment agent comprises an amine providing compound, an epoxy glue or
`
`solution, an acid solution, or ammonium acetate.
`
`Claim 3153. (New) The non-porous solid support of claim 3151, wherein said
`
`blocking agent comprises Denhardt's solution.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 4
`
`
`
`5tavrianopoulos et al..5. Pat. Appl. 5er. No. 08/486,07Ailed June 7, 1995)
`
`Page 5 [Amendment Under 37 C.F.R. §1 .116 .- June 30, 2004]
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`Claim 3154. (New) The non-porous solid support of claim 3144 or 3145, wherein
`
`said direct or indirect fixation or immobilization to said non-porous solid support is
`
`covalent.
`
`Claim 3155. (New) The non-porous solid support of claim 3144 or 3145, wherein
`
`said direct or indirect fixation or immobilization to said non-porous solid support is
`
`non-covalent.
`
`Claim 3156. (New) The non-porous solid support of claim 3144, wherein said
`
`single-stranded nucleic acid is indirectly fixed or immobilized to said non·porous solid
`
`support by sandwich hybridization or by hybridization to a complementary nucleic
`
`acid strand.
`
`Claim 3157. (New) The non-porous solid support of claim 3145, wherein one strand
`
`of said double-stranded nucleic acid is indirectly fixed or immobilized to said non·
`
`porous solid support by sandwich hybridization or by hybridization to a
`
`complementary nucleic acid strand.
`
`Claim 3158. (New) The non-porous solid support of claim 3144, wherein said
`
`nucleic acid comprises DNA or RNA.
`
`Claim 3159. (New) The non-porous solid support of claim 3145, wherein said
`
`nucleic acid comprises DNA, RNA or both.
`
`Claim 3160. (New) The non-porous solid support of claim 3144, wherein said
`
`nucleic acid comprises a nucleic acid sequence complementary to a nucleic acid
`
`sequence of interest sought to be identified, quantified or sequenced.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 5
`
`
`
`Stavrianopoulos et al..s. Pat. Appl. Ser. No. 08/486,07I=iled June 7, 1995)
`
`Page 6 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
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`Claim 3161. (New) The non-porous solid support of claim 3145, wherein one strand
`
`of said double-stranded nucleic acid comprises a nucleic acid sequence of interest
`
`sought to be identified, quantified or sequenced.
`
`Claim 3162. (New) The non-porous solid support of claim 3144, wherein said
`
`single-stranded nucleic acid is unlabeled.
`
`Claim 3163. (New The non-porous solid support of claim 3144 or 3145, wherein
`
`said non-porous solid support is transparent or translucent.
`
`Claim 3164. (New) The non-porous solid support of claim 3145, wherein said non(cid:173)
`
`radioactive chemical label is quantifiable in or from a fluid or solution, said quantity
`
`being proportional to the amount or quantity of said label or labels.
`
`Claim 3165. (New) The non-porous solid support of claim 3145, wherein said non(cid:173)
`
`porous solid support is transparent or translucent, and said non-radioactive chemical
`
`label is quantifiable in or from a fluid or solution or in or through said non-porous solid
`
`support, said quantity being proportional to the amount or quantity of said label or
`
`labels.
`
`Claim 3166. (New) The non-porous solid support of claim 3145, wherein said non(cid:173)
`
`radioactive chemical label comprises a chromagen or a chromagenic compound, a
`
`colored dye compound, a fluorogen or a fluorescent compound, a chemiluminescent
`
`compound, a chelating compound, an enzyme or an enzymatic compound, a
`
`coenzyme, biotin, iminobiotin, a hapten or a ligand.
`
`Enz~7(P)(C3)
`
`Exhibit 2020 Page 6
`
`
`
`Stavrianopoulos e~ al..s. Pat. Appl. Ser. No. 08/486,o7tiled June 7, 1995)
`
`Page 7 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3167. (New) The non-porous solid support of claim 3145, wherein a non(cid:173)
`
`radioactive signal from said non-radioactive chemical label is quantifiable or detectable
`
`by photometric techniques, spectrophotometric techniques, colorimetric techniques,
`
`fluorometric techniques or chemiluminescent techniques.
`
`Claim 3168. (New) The non-porous solid support of claim 3144, comprising more
`
`than one single-stranded nucleic acid.
`
`Claim 3169. (New) The non-porous solid support of claim 3145, comprising more
`
`than one double-stranded nucleic acid.
`
`Claim 3170. (New) A set comprising the non-porous solid support of claim 3144.
`
`Claim 3171. (New) A set comprising the non-porous solid support of claim 3145.
`
`Claim 3172. (New) A system comprising a non-porous solid support and at least one
`
`single-stranded nucleic acid directly or indirectly fixed or immobilized thereto in
`
`hybridizable form, wherein when said nucleic acid is indirectly fixed or immobilized to
`
`said non-porous solid support, said indirect fixation or immobilization is not to a cell
`
`fixed in situ to said non-porous solid support.
`
`Claim 3173. (New) A system comprising a non-porous solid support and at least one
`
`double-stranded nucleic acid fixed or immobilized thereto, wherein at least one nucleic
`
`acid strand of said double-stranded nucleic acid comprises at least one non(cid:173)
`
`radioactive chemical label which is quantifiable or detectable, and wherein when said
`
`nucleic acid is indirectly fixed or immobilized to said non-porous solid support, said
`
`indirect fixation or immobilization is not to a cell fixed in situ to said non-porous solid
`
`support.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 7
`
`
`
`Stavrianopoulos et al..s. Pat. Appl. Ser. No. 08/486,071i1ed June 7, 1995)
`
`Page 8 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3174. (New) The system of claim 3172 or 3173, wherein said non-porous
`
`solid support comprises glass or plastic.
`
`Claim 3175. (New) The system of claim 3172 or 3173, wherein said non-porous
`
`solid support comprises a plate, a well or wells, a microtiter well or microtiter wells,
`
`depressions, tubes, cuvettes, beads, or a set of said plates, wells, depressions, tubes,
`
`cuvettes or beads.
`
`Claim 3176. (New) The system of claim 3172 or 3173, wherein said non-porous
`
`solid support comprises more than one surface.
`
`Claim 3177. (New) The system of claim 3172 or 3173, wherein said non-porous
`
`solid support comprises reactive sites or binding sites thereon, wherein said nucleic
`
`acid is fixed or immobilized to one of said reactive sites or binding sites.
`
`Claim 3178. (New) The system of claim 3177, wherein said reactive sites or binding
`
`sites comprise one or more amines, hydroxyls or epoxides.
`
`Claim 3179. (New) The system of claim 3172 or 3173, wherein said non-porous
`
`solid support has been treated with a surface treatment agent, a blocking agent, or
`
`both.
`
`Claim 3180. (New) The system of claim 3179, wherein said surface treatment
`
`agent comprises an amine providing compound, an epoxy glue or solution, an acid
`
`solution or ammonium acetate.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 8
`
`
`
`5tavrianopoulos et al..5. Pat. Appl. 5er. No. 08/486,071=iled June 7, 1995)
`
`Page 9 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3181. (New) The system of claim 3179, wherein said blocking agent
`
`comprises Denhardt's solution.
`
`Claim 3182. (New) The system of claim 3172 or 3173, wherein said direct or
`
`indirect fixation or immobilization to said non-porous solid support is covalent.
`
`Claim 3183. (New) The system of claim 3172 or 3173, wherein said direct or
`
`indirect fixation or immobilization to said non-porous solid support is non-covalent.
`
`Claim 3184. (New) The system of claim 3172, wherein said single-stranded nucleic
`
`acid is indirectly fixed or immobilized to said non-porous solid support by sandwich
`
`hybridization or by hybridization to a complementary nucleic acid strand.
`
`Claim 3185. (New) The system of claim 3173, wherein said one strand of said
`
`double-stranded nucleic acid is indirectly fixed or immobilized to said non-porous solid
`
`support by sandwich hybridization or by hybridization to a complementary nucleic
`
`acid strand.
`
`Claim 3186. (New) The system of claim 3172, wherein said nucleic acid comprises
`
`DNA or RNA.
`
`Claim 3187. (New) The system of claim 3173, wherein said nucleic acid comprises
`
`DNA, RNA, or both.
`
`Claim 3188. (New) The system of claim 3172, wherein said nucleic acid comprises
`
`a nucleic acid sequence complementary to a nucleic acid sequence of interest sought
`
`to be identified, quantified or sequenced.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 9
`
`
`
`8tav;ianopoulos et al..8. Pat. Appl. 8er. No. 08/486,071iled Ju~e 7, 1995)
`
`Page 10 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3189. (New) The system of claim 3173, wherein one strand of said double(cid:173)
`
`stranded nucleic acid comprises a nucleic acid sequence of interest sought to be
`
`identified, quantified or sequenced.
`
`Claim 3190. (New) The system of claim 3172, wherein said single-stranded nucleic
`
`acid is unlabeled.
`
`Claim 3191. (New) The system of claim 3172 or 3173, wherein said non-porous
`
`solid support is transparent or translucent.
`
`Claim 3192. (New) The system of claim 3173, wherein said non-radioactive
`
`chemical label is quantifiable in or from a fluid or solution, said quantity being
`
`proportional to the amount or quantity of said label or labels.
`
`Claim 3193. (New) The system of claim 3173, wherein said non-porous solid
`
`support is transparent or translucent, and said non-radioactive chemical label is
`
`quantifiable in or from a fluid or solution or in or through said non-porous solid
`
`support, said quantity being proportional to the amount or quantity of said label or
`
`labels.
`
`Claim 3194. (New) The system of claim 3173, wherein said non-radioactive
`
`chemical label comprises a chromagen or a chromagenic compound, a colored dye
`
`compound, a fluorogen or a fluorescent compound, a chemiluminescent compound, a
`
`chelating compound, an enzyme or an enzymatic compound, a coenzyme, biotin,
`
`iminobiotin, a hapten or a ligand.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 10
`
`
`
`Stavrianopoulos et al., • . Pat. Appl. Ser. No. 08/486'07~iled June 7, 1995)
`
`Page 11 [Amendment Under 37 C.F.R. §1. 116 -- June 30, 2004]
`
`Claim 3195. (New) The system of claim 3173, wherein a non-radioactive signal
`
`from said non-radioactive chemical label is quantifiable or detectable by photometric
`
`techniques, spectrophotometric techniques, colorimetric techniques, fluorometric
`
`techniques or chemiluminescent techniques.
`
`Claim 3196. (New) The system of claim 3172, comprising more than one single(cid:173)
`
`stranded nucleic acid.
`
`Claim 3197. (New) The system of claim 3173, comprising more than one double(cid:173)
`
`stranded nucleic acid.
`
`Claim 3198. (New) An array comprising various single-stranded nucleic acids
`
`directly or indirectly fixed or immobilized in hybridizable form to a non-porous solid
`
`support, wherein when said nucleic acids are indirectly fixed or immobilized to said
`
`non-porous solid support, said indirect fixation or immobilization is not to a cell fixed
`
`in situ to said non-porous solid support.
`
`Claim 3199. (New) An array comprising various double-stranded nucleic acids
`
`directly or indirectly fixed or immobilized to a non-porous solid support, wherein at
`
`least one nucleic acid strand of said various double-stranded nucleic acids comprises
`
`at least one non-radioactive chemical label which is quantifiable or detectable, and
`
`wherein when said nucleic acids are indirectly fixed or immobilized to said non-porous
`
`solid support, said indirect fixation or immobilization is not to a cell fixed in situ to
`
`said non-porous solid support.
`
`Claim 3200. (New) The array of claim 3198 or 3199, wherein said non-porous solid
`
`support comprises glass or plastic.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 11
`
`
`
`Stavrianopoulos et al., • . Pat. Appl. Ser. No. 08/486,071i1ed June 7, 1995)
`
`Page 12 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3201. (New) The array of claim 3198 or 3199, wherein said non-porous solid
`
`support comprises a plate, a well or wells, a microtiter well or microtiter wells,
`
`depressions, tubes, cuvettes, beads, or a set of said plates, wells, depressions, tubes,
`
`cuvettes or beads.
`
`Claim 3202. (New) The array of claim 3198 or 3199, wherein said non-porous solid
`
`support comprises more than one surface.
`
`Claim 3203. (New) The array of claim 3198 or 3199, comprising reactive sites or
`
`binding sites thereon, wherein said nucleic acids are fixed or immobilized to said
`
`reactive sites or binding sites.
`
`Claim 3204. (New) The array of claim 3203, wherein said reactive sites or binding
`
`sites comprise one or more amines, hydroxyls or epoxides.
`
`Claim 3205. (New) The array of claim 31 98 or 31 99, wherein said non-porous solid
`
`support has been treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3206. (New) The array of claim 3205, wherein said surface treatment agent
`
`comprises an amine providing compound, an epoxy glue or solution, an acid solution
`
`or ammonium acetate.
`
`Claim 3207. (New) The array of claim 3205, wherein said blocking agent comprises
`
`Denhardt's solution.
`
`Claim 3208. (New) The array of claim 3198 or 3199, wherein said direct or indirect
`
`fixation or immobilization to said non-porous solid support is covalent
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 12
`
`
`
`Stavrianopoulos et al._. Pat. Appl. Ser. No. 08/486,07!iled June 7, 1995)
`
`Page 13 [Amendment Under 37 C.F.R. §1 .116 -- June 30, 2004]
`
`Claim 3209. (New) The array of claim 3198 or 3199, wherein said direct or indirect
`
`fixation or immobilization to said non-porous solid support is non-covAlent.
`
`Claim 3210. (New) The array of claim 3198, wherein said single-stranded nucleic
`
`acids or sequences are indirectly fixed or immobilized to said non-porous solid support
`
`by sandwich hybridization or by hybridization to complementary nucleic acid strands.
`
`Claim 3211. (New) The array of claim 3199, wherein one strand of said double(cid:173)
`
`stranded nucleic acids or sequences is indirectly fixed or immobilized to said non(cid:173)
`
`porous solid support by sandwich hybridization or by hybridization to complementary
`
`nucleic acid strands.
`
`Claim 3212. (New) The array of claim 3198, wherein said nucleic acids comprise
`
`DNA or RNA.
`
`Claim 3213. (New) The array of claim 3199, wherein said nucleic acids comprise
`
`DNA, RNA, or both.
`
`Claim 3214. (New) The array of claim 3198, wherein said nucleic acids comprise
`
`nucleic acid sequences complementary to nucleic acid sequences of interest sought
`
`to be identified, quantified or sequenced.
`
`Claim 3215. (New) The array of claim 3199, wherein one strand of said double(cid:173)
`
`stranded nucleic acids comprises a nucleic acid sequence of interest sought to be
`
`identified, quantified or sequenced.
`
`Claim 3216. (New) The array of claim 3198, wherein said single-stranded nucleic
`
`acids are unlabeled.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 13
`
`
`
`Stavrianopoulos et al._. Pat. Appl. Ser. No. 08/486,o7!iled June 7, 1995}
`
`Page 14 [Amendment Under 37 C.F.R. §1. 116 -- June 30, 2004]
`
`Claim 3217. (New) The array of claim 3198 or 3199, wherein said non-porous solid
`
`support is transparent or translucent.
`
`Claim 3218. (New) The array of claim 3199, wherein said non-radioactive chemical
`
`label is quantifiable in or from a fluid or solution, said quantity being proportional to
`
`the amount or quantity of said label or labels.
`
`Claim 3219. (New) The array of claim 3199, wherein said non-porous solid support
`
`is transparent or translucent, and said non-radioactive chemical label is quantifiable in
`
`or from a fluid or solution or in or through said non-porous solid support, said quantity
`
`being proportional to the amount or quantity of said label or labels.
`
`Claim 3220. (New) The array of claim 3199, wherein said non-radioactive chemical
`
`label comprises a chromagen or a chromagenic compound, a colored dye compound,
`
`a fluorogen or a fluorescent compound, a chemiluminescent compound, a chelating
`
`compound, an enzyme or an enzymatic compound, a coenzyme, biotin, iminobiotin, a
`
`hapten or a ligand.
`
`Claim 3221. (New) The array of claim 3199, wherein a non-radioactive signal from
`
`said non-radioactive chemical label is quantifiable or detectable by photometric
`
`techniques, spectrophotometric techniques, colorimetric techniques, fluorometric
`
`techniques or chemiluminescent techniques.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 14
`
`
`
`Stavrianopoulos et al..s. Pat. Appl. Ser. No. 08/486,07!iled June 7, 1995)
`
`Page 15 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3222. (New) An array comprising various single-stranded nucleic acids
`l
`directly or indirectly fixed or immobilized in hybridizable form to a nor.-porous solid
`
`support having wells or depressions, wherein when said nucleic acids are indirectly
`
`fixed or immobilized to said wells or depressions of said non-porous solid support,
`
`said indirect fixation or immobilization is not to a cell fixed in situ to said wells or
`
`depressions of said non-porous solid support.
`
`Claim 3223. (New) An array comprising various double-stranded nucleic acids
`
`directly or indirectly fixed or immobilized to a non-porous solid support having wells or
`
`depressions, wherein at least one nucleic acid strand of said various double-stranded
`
`nucleic acids comprises at least one non-radioactive chemical label which is
`
`quantifiable or detectable, and wherein when said nucleic acids are indirectly fixed or
`
`immobilized to said wells or depressions of said non-porous solid support, said indirect
`
`fixation or immobilization is not to a cell fixed in situ to said wells or depressions of
`
`said non-porous solid support.
`
`Claim 3224. (New) The array of claim 3222 or 3223, wherein said non-porous solid
`
`support comprises glass or plastic.
`
`Claim 3225. (New) The array of claim 3222 or 3223, wherein said wells or
`
`depressions comprise a plate of wells or depressions, or a microtiter plate of wells or
`
`depressions.
`
`Claim 3226. (New) The array of claim 3222 or 3223, comprising reactive sites or
`
`binding sites thereon, wherein said nucleic acids are fixed or immobilized to said
`
`reactive sites or binding sites.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 15
`
`
`
`Stavrianopoulos et al._. Pat. Appl. Ser. No. 08/486,o7!iled June 7, 1995)
`
`Page 16 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3227. (New) The array of claim 3226, wherein said reactive sites or binding
`
`sites comprise one or more amines, hydroxyls or epoxides.
`
`Claim 3228. (New) The array of claim 3222 or 3223, wherein said non-porous solid
`
`support has been treated with a surface treatment agent, a blocking agent, or both.
`
`Claim 3229. (New) The array of claim 3228, wherein said surface treatment agent
`
`comprises an amine providing compound, an epoxy glue or solution, an acid solution
`
`or ammonium acetate.
`
`Claim 3230. (New) The array of claim 3228, wherein said blocking agent comprises
`
`Denhardt's solution.
`
`Claim 3231. (New) The array of claim 3222 or 3223, wherein said direct or indirect
`
`fixation or immobilization to said non-porous solid support is covalent.
`
`Claim 3232. (New) The array of claim 3222 or 3223, wherein said direct or indirect
`
`fixation or immobilization to said non-porous solid support is non-covalent.
`
`Claim 3233. (New) The array of claim 3222, wherein said single-stranded nucleic
`
`acids are indirectly fixed or immobilized to said non-porous solid support by sandwich
`
`hybridization or by hybridization to complementary nucleic acid strands.
`
`Claim 3234. (New) The array of claim 3223, wherein one strand of said double(cid:173)
`
`stranded nucleic acids is indirectly fixed or immobilized to said non-porous solid
`
`support by sandwich hybridization or by hybridization to complementary nucleic acid
`
`strands.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 16
`
`
`
`~
`. '
`Stavrianopoulos et al.'s. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`Page 17 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3235. (New) The array of claim 3222, wherein said nucleic acids comprise
`
`DNA or RNA.
`
`Claim 3236. (New) The array of claim 3223, wherein said nucleic acids comprise
`
`DNA, RNA, or both.
`
`Claim 3237. (New) The array of claim 3222, wherein said nucleic acids comprise
`
`nucleic acid sequences complementary to nucleic acid sequences of interest sought
`
`to be identified, quantified or sequenced.
`
`Claim 3238. (New) The array of claim 3223, wherein one strand of said double(cid:173)
`
`stranded nucleic acids comprises a nucleic acid sequence of interest sought to be
`
`identified, quantified or sequenced.
`
`Claim 3239. (New) The array of claim 3223, wherein said nucleic acid sequence of
`
`interest sought to be identified, quantified or sequenced comprises a gene sequence
`
`or pathogen sequence.
`
`Claim 3240. (New) The array of claim 3222, wherein said single-stranded nucleic
`
`acids are unlabeled.
`
`Claim 3241. (New) The array of claim 3222 or 3223, wherein said non-porous solid
`
`support is transparent or translucent.
`
`Claim 3242. (New) The array of claim 3223, wherein said non-radioactive chemical
`
`label is quantifiable in or from a fluid or solution, said quantity being proportional to
`
`the amount or quantity of said label or labels.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 17
`
`
`
`Stav,ianopoulos et al., • . Pat. Appl. Se,. No. o8/486,o7c1led June 7, 1995)
`
`Page 18 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3243. (New) The array of claim 3223, wherein said non-porous solid support
`
`is transparent or translucent, and said non-radioactive chemical label is quantifiable in
`
`or from a fluid or solution or in or through said non-porous solid support, said quantity
`
`being proportional to the amount or quantity of said label or labels.
`
`Claim 3244. (New) The array of claim 3223, wherein said non-radioactive chemical
`
`label comprises a chromagen or a chromagenic compound, a colored dye compound,
`
`a fluorogen or a fluorescent compound, a chemiluminescent compound, a chelating
`
`compound, an enzyme or an enzymatic compound, a coenzyme, biotin, iminobiotin, a
`
`hapten or a ligand.
`
`Claim 3245. (New) The array of claim 3223, wherein a non-radioactive signal from
`
`said non-radioactive chemical label is quantifiable or detectable by photometric
`
`techniques, spectrophotometric techniques, colorimetric techniques, fluorometric
`
`techniques or chemiluminescent techniques.
`
`Claim 3246. (New) A non-porous glass or plastic solid support comprising at least
`
`one single-stranded nucleic acid directly or indirectly fixed or immobilized thereto in
`
`hybridizable form, wherein when said single-stranded nucleic acid is indirectly fixed or
`
`immobilized to said non-porous glass or plastic solid support, said ind:rect fixation or
`
`immobilization is not to a cell fixed in situ to said non-porous glass or plastic solid
`
`support.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 18
`
`
`
`,
`Stavrianopoulos et al.fs. Pat. Appl. Ser. No. 08/486,070 (Filed June 7, 1995)
`
`•
`
`Page 19 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3247. (New) A non-porous glass or plastic solid support comprising at least
`
`one double-stranded nucleic acid directly or indirectly fixed or immobilized thereto,
`
`wherein at least one nucleic acid strand of said double-stranded nucleic acid
`
`comprises at least one non-radioactive chemical label which is quantifiable or
`
`detectable, and wherein when said double-stranded nucleic acid is indirectly fixed or
`
`immobilized to said non-porous glass or plastic solid support, said indirect fixation or
`
`immobilization is not to a cell fixed in situ to said non-porous glass or plastic solid
`
`support.
`
`Claim 3248. (New) The non-porous glass or plastic solid support of claim 3246 or
`
`3247, wherein said non-porous glass or plastic solid support comprises a plate, a well
`
`or wells, a microtiter well or microtiter wells, depressions, tubes, cuvettes, beads, or
`
`a set of said plates, wells, depressions, tubes, cuvettes or beads.
`
`Claim 3249. (New) The non-porous glass or plastic solid support of claim 3246 or
`
`3247, wherein said non-porous solid support comprises more than one surface.
`
`Claim 3250. (New) The non-porous glass or plastic solid support of claim 3246 or
`
`3247, comprising reactive sites or binding sites thereon, wherein said nucleic acid is
`
`fixed or immobilized to one of said reactive sites or binding sites.
`
`Claim 3251. (New) The non-porous glass or plastic solid support of claim 3250,
`
`wherein said reactive sites or binding sites comprise one or more amines, hydroxyls or
`
`epoxides.
`
`Claim 3252. (New) The non-porous glass or plastic solid support of ,claim 3246 or
`
`3247, wherein said non-porous glass or plastic solid support has been treated with a
`
`surface treatment agent, a blocking agent, or both.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 19
`
`
`
`Stavrianopoulos et al . • s. Pat. Appl. Ser. No. 08/486,o7!iled June 7, 1995)
`
`Page 20 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3253. (New) The non-porous glass or plastic solid support of claim 3252,
`
`wherein said surface treatment agent comprises an amine providing compound, an
`
`epoxy glue or solution, an acid solution or ammonium acetate.
`
`Claim 3254. (New) The non-porous glass or plastic solid support of claim 3252,
`
`wherein said blocking agent comprises Denhardt's solution.
`
`Claim 3255. (New) The non-porous glass or plastic solid support of claim 3246 or
`
`3247, wherein said direct or indirect fixation or immobilization to said non-porous
`
`solid support is covalent
`
`Claim 3256. (New) The non-porous glass or plastic solid support of claim 3246 or
`
`3247, wherein said direct or indirect fixation or immobilization to said non-porous
`
`solid support is non-covalent.
`
`Claim 3257. (New) The non-porous glass or plastic solid support of claim 3246,
`
`wherein said single-stranded nucleic acid is indirectly fixed or immobilized to said non(cid:173)
`
`porous glass or plastic solid support by sandwich hybridization or by hybridization to a
`
`complementary nucleic acid strand.
`
`Claim 3258. (New) The non-porous glass or plastic solid support of claim 3247,
`
`wherein one strand of said double-stranded nucleic acid is indirectly fixed or
`
`immobilized to said non-porous glass or plastic solid support by sandwich
`
`hybridization or by hybridization to a complementary nucleic acid strand.
`
`Claim 3259. (New) The non-porous glass or plastic solid support of claim 3246,
`
`wherein said nucleic acid or sequence comprises DNA or RNA.
`
`Enz-7(P)(C3)
`
`Exhibit 2020 Page 20
`
`
`
`Stavrianopoulos et al.'s. Pat. Appl. Ser. No. 08/486,o7!iled June 7, 1995)
`
`Page 21 [Amendment Under 37 C.F.R. §1.116 -- June 30, 2004]
`
`Claim 3260. (New) The non-porous glass or plastic solid support of claim 3247,
`
`wherein said nucleic acid or sequence comprises DNA, RNA, or both.
`
`Claim 3261. (New) The non-porous glass or plastic solid support of claim 3246,
`
`wherein said nucleic acid comprises a nucleic acid sequence complementary to a
`
`nucleic acid sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3262. (New) The non-porous glass or plastic solid support of claim 3247,
`
`wherein one strand of said double-stranded nucleic acid comprises a nucleic acid
`
`sequence of interest sought to be identified, quantified or sequenced.
`
`Claim 3263. (New) The non-porous glass or plastic solid support of claim 3246,
`
`wherein said single-stranded nucleic acid is unlabeled.
`
`Claim 3264. (New) The non-porous glass or plastic solid support of claim 3246 or
`
`3247, wherein said non-porous glass or plastic solid support is transparent or
`
`translucent.
`
`Claim 3265. (New) The non-porous glass or plastic solid support of claim 3247,
`
`wherein said non-radioactive chemical label is quantifiable in or from a fluid or
`
`solution, said quantity being proportional to the amount or quantity of said label or
`
`labels.
`
`Enz-7(PHC3)
`
`Exhibit 2020 Page 21
`
`
`
`Stavrianopoulos et al..s. Pat. Appl. Ser. No. 08/486,o7!iled June 7, 1995)
`
`Page 22 [Amendment Under 37 C.F