Please complete this Form [in type (English) and send with five additional photocopies by January 31, 1983!:
`Form 11, III, and W should be mailed with this Form 1.
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`Please indicate your preference of workshop number.
`lst choice No.
`105
`2nd choice No.
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`3rd choice No.
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`Expression of cloned immunoglobulin genes in heterologous cells
`
`m
`Author(s): Please underline speaker's name
`Hawley and M.J. Shulman
`N. Hozumi, A. Ochi,
`_—__—+_______________________,""——
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`—-—v———-—-—-—-——-—‘——_—,_—____u
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`The Ontario Cancer Institute and Department of Medical
`Affiliation OfAuthO'fs)
`Biophysics, University of Toronto, Toronto, M4X 1K9 Canada
`
`Abstract: (single space)
`fi——“_‘——_‘—“_—‘H—"————r—vr——fi
`Transfer of cloned genes iuLo tissue culture cells has been shown tc-
`be a useful strategy for the study of the mammalian gene expression.
`To this
`end a mouse immunoglobulin k light chain gene specific for the hapten 2,4,6
`trinitrophenyl
`(TNP) has been inserted into a transducing vector (pSVZ-neo)
`I carrying the bacterial phosphotransferase gene (neo) which confers resistance
`to the antibiotic G418.
`The recombinant plasmids were thethransferred into a
`mutant hybridoma cell line (igk—lA) which was known to be missing the TNP
`specific k light chain gene by the method of protoplast fusion (W. Schaffner,
`Proc. Natl. Acad. Sci. USA, 77, p 2167, 1980). The G418 resistant transform-
`ants were assayed for secreti—on of functional antibody by restoration of (anti-
`TNP plaqueformation. Our system demonstrated that a cloned k light chaiin
`capable of restoring specific antibody production to the transformed cells.
`(Ochi gal. Nature (in press)). The results of the transfer of light chain
`{I‘VI heavy chefs genes into sever-".1. other cell Jines, inrlriirg T cel‘s, pre—B
`cells, and fibroblasts, will be discussed. The work was supported by grants
`from the Medical Research Council of Canada,
`the National Cancer Institute of
`suede and the
`llstate Foundation.
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`
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`Others(
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`Please type the names of all Authors in full for Author Index. (Check the box to indicate the Speaker)
`F3111in name
`First name Middle initial
`Family name
`First name
`Robert
`/
`G .
`Nobumichi
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`Cl
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`/
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`Middle initial
`/
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`Correspondence: Name and Mailing address
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`Dr. N. Hozumi, Ontario Cancer Institute. 500 Sherbourne St., Toronto, Ont. MAX 1K9
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`made—m
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`Mylan v. Genentech
`IPR2016-00710
`Merck Ex. 1140, Pg. 1
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