Gfinter Kahl
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`Genomics, Transcriptomics, Proteomics
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`Second Edition
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`The Dictionary of
`Gene Technology
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`Weinheim - New Turk - Chiehester - Brisbane - Singapore ' Terente
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`@WILEY-VCH
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`Mylan v. Genentech
`IPR2016-00710
`Merck Ex. 1109, Pg. 1
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`Cover Illustration:
`The Title Page shows a genomic fingerprint produeed by —v selective amplification of microsa-
`tellttc polymorphic loci (SAMPL), a high-density —1 expression array and —- fluorescence in situ
`hybridization images of plant chromosomes on a background of cross-references to the entry _.
`polymerase chain reaction (PCB). These photos were kindly provided by Drs. Bruno Hiittel and
`Christina Staginnus (Plant Molecular Biology, Biocentre of Frankfurt University} and Gene
`Scan Europe AG, Freiburg, Germany.
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`
`
` Professor Dr. Giinter Kahl
`Plant Molecular Biology
`Johann Wolfgang Goethe University
`Biocentre
`
`Marie-Curie-Strafle 9
`
`III—60439 Frankfurt am Main
`Gert'nany
`Phone: +49(069)—?932—926?
`Fan:
`+49{069}—?932—9263
`e-mail: kahl@em.uni—l‘rankfurt.de
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`
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`‘This book was carefully produced. Nevertheless, author and publisher do not warrant the
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`information contained therein to be free of errors. Readers are advised to keep in mind that
`statements, data, illustrations, procedural details or other items may inadvertently be inaccurate.
`
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`Library of Congress Card No. applied for
`
`A catalogue record for this book is available from the British Library
`
`Die Deutsche Bibliothek Cataloguing-in-Publication Data:
`A catalogue record for this publication is available from Die Deutsche Bibliothek
`ISBN 3—52?—30100—3
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`Wiley-VCH Verlag GmbH, 13—69469 Weinheim (Federal Republic of Germany}, 2001
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`Printed on acid-free paper.
`
`All rights reserved (including these oftranslation into other languages). No part ofthis book may
`be reproduced in any form # by photoprinting, microfilm or any other means — nor traosmrtted or
`translated into a machine language without written permission from the publishers. Registewd
`names, trade-marks, etc. used in this book, even when not specifically marked as such, are not to
`be considered unprotected by law.
`
`Composition: pagina GmbH, Tflbingen
`Printing: Strauss fosctdruck, Morlenbach
`Bookbinding: J. Schaffer, Griinstadt
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`L
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`Printed in the Federal Republic of Germany
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`Merck Ex. 1109, Pg. 2
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`Multiplex fluorescent in silu hybridization 511
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`u —
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`-
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`A supramolecular complex consisting of two or more identical or non identical s b
`]
`.-
`mers). For esamp e, a protein molecule. made up of two or more individual poly
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`Mlflflnyel'l
`units {mi-"mi?
`Peptide chains.
`Multiple allelisrn: The occurrence ofmore than two alleles ofa genomic .3. Imus in a pflpulatifln
`Mulfiph arbitrary rinlplienn profiling (MAAP): See —1 arbitrarily amplified DNA.
`Multiple cloning site (MCS): Synonym for —i polylinker.
`Mulfiplefiuorescence—based PIPE-88C!” (MF—PCR-SSCP}: A sensitive mutation detection tech-
`nique that combines conventional —i polymerase chain reaction with two {or more} primers
`labeled with different
`-—i fluoroehromes and —i single-strand conformation polymorphism
`analysis. In short. the target sequence is first amplified using forward and reverse primers labeled
`with two different dyes. respectively (e. g. FAM [blue] and JOE [green]} at their fi'nends. Mpli-
`fied products are then heat-denatured, mixed with an internal DNA size marker labeled with a
`third dye (e. g. ROX [red]), and run in temperature-controlled non-denaturing —.i polyaeryla-
`NA sequencer. Mutations are detected as positional shifts of two-
`rnide gels in an automated D
`coloured peaks in the electropherogram. The technique allows to diagnose single base enchanges
`and —l- loss of heterozygosity, and works without radioactivity.
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`Multiple gene DNA shuffling: See -—:i DNA shuffling.
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`Multiple hit: See —-:i superimposed substitution.
`ariant of the -:r PCR in situ hybridization. using —:i primers
`Multiple overlapping primer PCR: A v te large amplification products. that do not diffuse away
`with overlapping sequences to genera
`from their original location in cells or tissue specimens.
`Figure see page .5f2.
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`Wflple fififlE Northern blot {MTN blot}: A readyuto-hybridiaesues of an organism. separated by
`resis and blotted onto a nylon membrane. Equal amounts of
`denaturing —} aga re se gel electrophfl
`ssioo of genes.
`RNA per lane allow to detect tissue-specific exp”
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`_
`Hmultipleit DAF]:
`t least two or multiple primer o
`h polymorphisms.
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`_
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`I
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`{fiulfillles DNA amplificationfingerprin
`""1 fingerpfiflting technique that uses a
`Emmi-E —1 ainplifleation fragment lengt
`ue for the simultaneous detection
`Multiplesfloor-assent in situ hybridization (or-FISH}: A techniq
`mes in a metaphase spre- d by different colorieation.
`re first labeled with distinct combinations of
`.
`'1 5'10“, chromosome-specific DNA libraries a
`Elfluorochromfl Than thedifferent, specifically labeledehromosomal
`librariesarehybri-
`ontospreadsofmetaphasechromosomes(orcell nuclei). and theindivldual fluorochromeo
`Ema“ bl" Epifluoresoonoo microscopy (using all filters to excite all the fluorochromes in the
`impk} mupled to a cooled chargedcoupled device (CED) camera-
`“Incl: afluw l0 unequivocally assign a SP
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`illElia
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`Merck Ex. 1109, Pg. 3
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