`United States Patent
`(10) Number:
`US 6,331,415 C1
`Cabilly et al.
`(45) Certificate Issued:
`May 19, 2009
`
`US00633 l4l5Cl
`
`(54) METHODS OF PRODUCING
`llV[lV[UNOGl.OBUl,lNS, VECTORS AND
`TRANSFORMED HOST CELLS FOR USE
`
`(75)
`
`lnventorsz Shmuel Cabilly, Monrovia, CA (US);
`.
`Herbert-L-. Heyneker, Burlingarne, CA
`(US); William E. Holmes, Pacilica, CA
`(US); Arthur D. Riggs, T1aVerne, CA
`(US); Ronald B. VVetzel, San Francisco,
`CA (US)
`
`(73) Assignees: Genentech, Inc., South San Francisco,
`CA (Us), City of Hope, Duarte, CA
`(US)
`.
`.
`Reexamination Request:
`No. 90/007,542, May 13, 2005
`No. 90/007,859, Dec. 23, 2005
`Reexamination Certificate for:
`Patent NO.Z
`6,331,415
`Issued;
`Dec. 18, 2001
`Appl. No_;
`07/205,419
`
`4,792,447 A
`4,319,567 A
`4,965,196 A
`:=3:1a;:::
`2
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`5,179,017 A
`5,225,538 A
`5,336,603 A
`5,420,020 A
`5,428,130 A
`5,455,165 A
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`5,500,362 A
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`5,583,013 A
`5,585,089 A
`.
`‘§’2‘1’Z’?§‘§ 2
`5:648:37 A
`5,686,072 A
`57211103 A
`5,736,137 A
`5,807,715 A
`5,840,545 A
`,
`,._
`
`12/1988 Uhr et al.
`3/:1939 Cab_i11Y et 31‘
`10//1990 Levinson et 31.
`;1,1::2:1;a::;:1
`gape“ 6 a1’
`1
`allas
`1/1993 Axel et al.
`7/1993 C/apcn 6 31.
`8/1994 Capon e al.
`5/1995 Riggs
`6/1995 Capone al.
`10/1995 Capon e :11.
`
`3/1996 Robinson Ct 211.
`/
`FOVV Cy
`Eapolll e a1~
`12/1996 Itakura
`12/1996 Queene al.
`,
`.,
`311339 €}i11‘2?‘;".
`7,1997 Cm,
`.
`11/1997 U11‘
`t
`2/1993 R01,1::,n 91 31,
`4/1998 Anderson et al.
`9/1998 Morrison et al.
`ll/1998 Moorc
`1
`air e a .
`
`Certificate of Correction issued Jun. 25, 2002.
`Related U.S. Application Data
`_
`_
`_
`5
`_
`_
`(63) Continuation of application No. 06/483,457, filed on Apr. 8,
`1983’ “OW P‘1‘~N°« 4-»315s>‘57~
`Int_ CL
`C12]V15/13
`CNN 15/0”
`CIZN 15/63
`
`(2006.01)
`(2006-01)
`(2006.01)
`
`(51)
`
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`
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`zxilfifimn at al.
`2
`al.
`E1-£::r et
`2
`3
`9/2000 Robinson et al.
`6,120,767 A
`3/2001 Robinson et a1.
`6,204,023 B1
`12/2001 Cabilly etal.
`6,331,415 B1
`91/.3002 Aiiel et a1.
`6,453,275 B1
`41_003 Winter
`6,548,640 131
`FOREIGN PATENT DOCUMENTS
`0 036 776
`3/1981
`
`............... .. 435/69.6; 435/252.1; 435/252.3;
`(52) U.S. C1.
`435/252.33; 435/254.11; 435/254.2; 435/254.21;
`435/69.7; 435/70.21; 435/71.2; 435/71.1;
`435/70.1; 435/320.1; 435/455; 435/483;
`435/485; 435/471; 435/69.1
`h ...................... ..N
`585 F'ld fCl..'fi
`t’
`S
`)
`SQ: ap‘;)hCa*t':O:1l ficl: ‘ff:C::1l;ctC Search historv
`one
`"
`.
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`
`(Continued)
`~
`~
`~
`,
`~
`Przmaijt Examiner Padniashri Ponnaluri
`(57)
`ABSTRACT
`
`The invention relates to processes for producing, an ii11iiiuiio-
`globulin or an irnrnunologically functional iminurioglobulin
`fragment containing at lcast thc variable domains of thc
`irnmuno lobulin hea
`and light chains The rocesses can
`V
`g
`,h. _h
`d _
`‘b [1151 h V
`V
`1 d
`95° “He 9’ “M? V“ °“ “ 1° Pr.” '4‘? °
`6. ,°‘‘V.’ 4“
`l1glit chains or lragments thereot in a single cell. lhe inven-
`tion also relates to the vectors used to produce the immuno-
`globulin or fragment, and to cells transformed with the vec-
`tors.
`
`Sanofi/Regeneron Ex. 1026, pg 810
`
`Mylan Ex. 1026, pg 810
`
`
`
`US 6,331,415 C1
`Page 2
`
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`EP
`W0
`W0
`WO
`W0
`W0
`W0
`W0
`WO
`W0
`W0
`WO
`
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`10/1992
`93/07899
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`10/1993
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