G_L-U
`VAL
`GLN
`Leu
`VAL
`GLU
`sen
`GLV
`GLV
`u
`GLY
`GL
`GL
`sen
`Leu
`n
`
`A
`Y
`AL
`sen
`GLY
`H
`H
`
`dfu
`VAL
`GLN
`LEU
`VAL
`asp
`sen
`GLY
`GLY
`
`GLY
`
`dfu
`VAL
`G‘LN
`LEU
`leu
`GLU
`sen
`GLY
`GLY
`u
`GLY
`
`G_LU
`sfu Gfu Gfu
`VAL
`VAL
`VAL
`VAL
`GLN
`GLN
`em GLN
`Leu
`Leu
`LEU
`Leu
`Ieu
`Ieu
`VAL
`VAL
`GLU
`GLU
`GLU
`GLU
`sen
`sen
`sen
`sen
`GLY
`GLY
`GLY
`GLY
`GLY
`GLY
`GLY
`GLY
`u
`Eu
`Eu
`GLY
`GLY
`GLY
`L
`In
`L
`n
`L
`L
`en
`LEU
`G
`U
`n
`s
`A
`sen
`Y
`e
`Fl
`I
`
`GLY
`
`L
`LV
`sen
`LEU
`
`
`
`sen
`S
`
`H
`E
`
`sen
`GLV
`e
`R
`PH
`
`sen
`Y
`e
`
`G-l:U
`VAL
`GLN
`LEU
`VAL
`GLU
`sen
`GLY
`GLY
`
`GLY
`LN
`LY
`
`(3.1-._U
`VAL
`GLN
`LEU
`VAL
`GLU
`sen
`GLY
`GLY
`
`GLY
`
`sen
`LEU
`
`sen
`L
`H
`H
`
`G‘|-_-U
`VAL
`GLN
`LEU
`VAL
`GLU
`sen
`GLY
`GLY
`Leu
`GLY
`VAL
`GLN
`PRO
`GLY
`GLV
`5
`sen
`LEU
`LEU
`AHG Ans
`Leu
`LEU
`sen
`sen
`2:: El:
`ALA
`ALA
`sen
`sen
`Y
`LY
`E
`e
`F-I
`e
`_
`n
`'
`" L
`T
`R
`Tvn
`TYFI Asx
`sen
`THH
`AsN
`ALA
`TYR
`VAL MET MET MET
`Tvn
`ASN
`VAL
`
`A
`T
`ALA
`sen
`THR
`TRF
`ALA
`sen
`Ans MET MET
`PHE
`LYS
`sen
`
`.-
`TRP
`VAL
`- ‘
`
`III
`Tne
`VAL
`ARG
`
`.:.
`Tne
`VAL
`AFIG
`‘
`
`I
`TFIP
`VAL
`ARG
`
`L
`TRP
`VAL
`AFIG
`
`TRP
`VAL
`nno
`
`VAL
`VAL
`Ans Ans
`
`VAL
`VAL
`ARG Ans
`
`VAL
`ARG
`
`VAL
`Ans
`
`VAL
`Ans
`
`Leu
`Leu
`TVH
`T~Vn
`GLN As~
`L“ L“
`MET MET
`Leu
`ASN
`sen
`sen
`LEU
`LEU
`GLU Ans
`PRO ALA
`GLU
`GLU
`ASP
`ASP
`THR
`Tnn
`ALA
`ALA
`VAL
`VAL
`Tvn
`Tvn
`TYR
`TYR
`
`S1,?
`SE?
`ARG AF_IG
`VAL
`sen
`THR
`nno
`Pno
`VAL
`ALA
`sen
`ALA
`Leu
`ALA
`VAL
`sen
`ASP
`LEU
`GLY
`‘nan
`Tap
`-~
`Leu
`TYR
`_._
`Tvfi
`en
`EH5 GtY
`ALA
`SE9
`VA_L_ Wm
`LY
`TRP
`TRP
`G.
`LY
`gm
`,;;
`$1,“
`LEU
`LElJ
`VAL
`THR
`
`sen
`ALA
`Tvn
`TYR
`PHE
`
`THR
`ALA
`VAL
`ALA
`PHE
`
`
`
`nno
`TYR
`VAL
`sen
`nno
`THR
`nHe
`...
`...
`--
`PHE
`T:
`,,3J,l.$,_pEE3Q|-'lTMENT Q5 HEALTH
`;H§k‘||'liV|.|urn'n"1_\“L‘| SEuu|cEs
`WM VAL
`GLY
`GLV
`-
`-
`Tnn
`fin‘ ‘1'fiF'
`WE‘
`V
`-
`-
`GLN GLNP®lIc Health fifince
`$,fifi Mfitnoflfil lnslntutesfihfi Health
`LYS
`LEUI
`LEU
`LEU
`VAL
`VAL
`VAL
`VAL
`VAL
`QFR
`sen
`Tnn
`Tnn
`Tnn
`
`
`
`GLU
`GIL:
`GLU
`G]
`GLU
`VAL
`VAL
`VAL
`VA
`VAL
`am am GLN
`am an
`LEU
`LEU
`LEU
`LEU
`LE
`VAL
`VAL
`VAL
`VAL
`VI
`GLX
`GLU
`GLU
`GLX
`an
`sen
`sen
`thr
`sen
`se
`GLY
`GLY
`GLY
`GLY
`GI
`GLY
`GLY
`GLY
`GLY
`GI
`u
`LELJ
`Leu
`Leu
`LE
`«ELY
`GLY an GLY
`as
`VAL
`VAL
`He
`VAL
`VA
`Iys
`lys
`GLN
`GLX
`Gl
`nno
`nno
`PRO
`Pno
`ne
`GLY
`GLV
`GLY
`GLY
`ea
`GLY
`GLY
`GLY
`GLY
`an
`sen
`sen
`sen
`sen
`se
`Leu
`LEU
`LEU
`LEU
`LE
`Ans Ans
`ARG
`ARG AF
`LEU
`LEU
`LEU
`LEU
`LE
`sen
`sen
`sen
`gly
`ss
`ALA
`ALA
`ALA
`ALA
`Al
`cvs
`CYS
`CV5
`CYS
`cw
`ALA
`ALA
`ALA
`ALA
`AL
`sen
`sen
`sen
`sen
`se
`Y
`Va:
`GLY
`GLY
`Gl
`e we
`nne
`nne
`n+
`e
`THR
`THH
`as
`E
`PHE
`val
`Pr
`Bile
`sen
`nu
`Tun
`Asx
`Gl
`Tvn
`HIS
`T\
`VAL
`sen
`5
`MeT MET
`MI
`Asx
`H
`
`TYH
`
`sen
`Tnn
`VAL
`Ans
`
`_ _
`TF
`LE
`AF
`
`PF
`GI
`
`TF
`gr]
`ETr
`gt:
`ILTL
`T:
`
`A:G:
`G!
`s:
`V:L!‘|’\
`AK
`A:
`5:
`V!
`L.
`GT
`Ar
`
`E:
`ll
`SI
`
`ATAl
`G!L.
`TI
`Ll
`T‘
`
`E‘
`Ll
`A.‘
`A
`LI
`AT
`Pl
`s
`A:
`TT
`AT
`nu
`T‘
`T‘
`
`Au
`TVn
`
`EEK
`MET MET
`ASN
`ASN
`GLY AsN
`LEU
`LEL)
`GLN Ans
`ALA
`VAL
`GLU
`GLX
`VAL Asx
`sen
`THE
`ALA
`ALA
`ILE
`VAL
`TYR
`TYR
`TYR
`TYR
`
`VAL
`TYR
`
`#3.
`MET MET
`Asx
`ASN
`sen
`GLY
`LEU
`LEU
`Ane Ans
`ALA
`ALA
`GLU
`GLX
`ASP Asx
`THE
`GLY
`ALA
`GLY
`VAL
`VAL
`TYR
`Tvn
`TYR
`TVn
`
`SE? SE E?
`Ane
`sen
`L‘
`ASP Asx
`G
`LEU
`VAL
`T‘
`ALA
`u
`ALA
`TI
`ALA
`A1
`Ans
`<3
`LEU
`A:
`TA
`F1
`-
`
`
`
`--
`
`
`
`iii?
`2!?
`Ane”_A_n_s
`ASP Asx
`ALA Ans
`GLY
`LEU
`PRO GLY
`TYR
`PRO
`VAL Tnn
`sen
`ALA
`PRO cvs
`THFI
`SER
`PHE
`VAL
`- -
`——
`-
`._
`_
`...
`—
`P:E
`MET
`ASP Asp M
`7_>VVAL V& ms
`GLY
`GLY
`GLY
`TFIP
`TRP
`TRP
`GLY
`GLY
`GLY
`GLN
`GLN
`GLN
`W, W. Tm
`LEU
`nr-To
`new
`VAL
`VAL
`VNL
`sen
`THR
`THR
`
`V
`
`PHE
`
`GLY
`GLV
`LYS
`"....
`Tun
`VAL
`SFH
`
`A
`"g
`G
`T
`E
`A.
`L»
`V
`S
`Tn
`
`Genzyme Ex. 1026, pg 721
`
`‘T
`T
`
`LEU[ 99‘,
`
`sen: es)
`eLv(.99)
`GLY( 99)
`
`nno(.9sT
`GLY
`sen: am
`_LEU[9B:
`AFl(3(_95}
`
`cvs
`ALA( 95;
`sen( 97)
`GLv( 95)
`PHET 971
`
`’
`
`_
`‘-Tnn
`5AFlG( 951
`GLM95)
`_nno(ss)
`GLY
`
`LYS(.95)
`Tnn
`LEU[ 951
`
`L V
`VAL{95)
`
`I
`EAHG
`
`‘PT-Te
`IILE
`sen
`
`ARTS
`
`|Tvn
`
`I
`‘METT 95;
`>
`~
`
`5
`
`vn
`YR( 96)
`
`*5
`
`'
`
`;
`1
`
`V
`V
`
`r
`LHE
`»
`5
`E
`L
`L
`VAL
`EFR
`
`Genzyme Ex. 1026, pg 721
`
`

`
`
`
`
`
`SEQUENCES OF PROTEINS OF
`IMMUNOLOGICAL INTEREST
`
`Elvin A. Kahat, Tai Te Wu, Howard Bilofsky.
`Margaret Reid-Miller, and Harold Perry
`(1983)
`
`Page;
`
`Erzata
`
`20
`
`Position -1 of 1M5'CL (precursor heavy chains) should be Pbe.
`Pau and Paul are the same protein.
`
`10K26—12A1
`and
`The antibody specificities for 10KMU—TA1
`light chains) should by anti-p—azobenzene arsonate.
`and S175.
`SAPC178 and SAPC176 (mouse lambda light chains) should be named as
`
`(mouse
`
`kappa
`
`S178
`
`light
`lambda
`References for HOPC1, J698, H2061, S176, and H2020 (mouse
`chains)
`should be weigert, M., Cesari. I.M., Yonkovich. S.J. & Cobn, M.
`(1970) Nature, 228. 1DA5—10N7.
`
`65;
`
`651
`
`26
`27
`
`66;
`
`67;
`
`111;
`
`23
`
`168;
`168;
`
`30
`35
`
`1B5;
`
`52
`
`2A6;
`
`he
`should
`(mouse lambda light chains)
`References for H3159 and MDPCS11
`2112-2116.
`Cesari;
`I.M. & Weigert, M.
`(1973) Proc. Natl. Acad. Sci. U.S.A., 70.
`
`sequenced
`chains) were
`light
`lambda
`(mouse
`S178
`and
`J558. XS1DH.
`completely. while HOPC1,
`J69B, H2061. S176, H2020. W3159. and MOPCE11
`compositions.
`(mouse lambda
`light
`chains) were
`analyzed by
`amino
`acid sequence
`
`and MA8-13
`There is an additional reference to TEPC952
`light
`chains)
`i.e., Elliott,
`E.W., Jr.. Steiner, L.A.
`(1951) Fed. Proc., 40. 1098.
`
`lambda
`(mouse
`A Eisen, H.N.
`
`"The order of
`The statement in the notes of mouse lambda light chains,
`the
`genes has been determined as V1—J3-C3—J1-C1-V2-J2-C2-JR-CH." should
`J2-C2—JH—CH."
`be
`replaced by
`“There
`are
`two
`linkage groups:
`J3—C3-J1—C1
`and
`
`Positions 13 and 1A of CAM (human heavy chain subgroup
`Gln and Lys respectively.
`
`III)
`
`should
`
`be
`
`Position 171 of SU3'CL (light constant chain) should be Asn.
`be Ser
`Positions 1u2 and 143 or MOPC315 (light constant chain)
`should
`and Gly respectively. based on the translation from nucleotide sequences
`(flothwell. A.L.M., Paskind, M., Roth, M.,
`Imanishi-Kari. T., Rajewsky,
`K.
`& Baltimore, D.
`(1982) Nature, 298, 380-382; Hu, n.E., Govindi. N.,
`Hozumi. N. & Murialdo, H.
`(1982) Nucl. Acids Res.. 10, 3831-3843).
`Positions 258 and 263
`of MOPC173
`(heavy
`constant
`chain)
`have
`revised by the authors to Pro and Val respectively.
`The position numbering for the codons of
`light
`chain variable region
`Sh°U1d read as 95: 95A. 953. 95C, 95D. 95E. 95F. 96. and 97.
`
`been
`
`ii
`ILE
`ATC
`THR
`ACC
`PHE
`TTC
`GGC GLY
`CAA GLN
`GGG
`GLY
`ACA
`THR
`CGA
`ARG
`CTG
`LEU
`GAG
`GLU
`ATT
`ILE
`AAA
`LYS
`CGT
`ARG
`
`P.
`
`Genzyme Ex. 1026, pg 722
`
`
`
`
`
`The human kappa J-segments (Hieter, P.A., Maizel, J.V., Jr. & Leder,
`(1982) J. Biol. chem.. 257, 1516-1522) are as follows:
`J1
`J2
`J3
`J5
`TRP
`LEU
`PHE
`TGG
`TAC
`TTC
`CTC
`THR
`THE
`THE
`ACG
`ACT
`ACT
`ACT
`PHE
`PHE
`TTC
`TTT
`TIC PHE
`TTC
`GLY
`GGC
`GGC
`GGC
`GLY
`GGC GLY
`GLN
`GAA
`GAG
`CCT
`PRO
`GGA
`GLY
`GLY
`GGG
`GGG
`GGG
`GLY
`GGG
`GLY
`THR
`ACC
`ACC
`ACC
`THR
`ACC
`THR
`LYS
`AAG
`AAG
`AAA
`LYS
`AAG
`LYS
`VAL
`GT6
`CTG
`GTG
`VAL
`GTG
`VAL
`GAA GLU
`GAG
`GAT ASP
`GAG
`GLU
`ATC
`ILE
`ATC
`ATC
`ILE
`ATC
`ILE
`AAA
`LYS
`AAA
`AAA
`LYS
`AAA
`LYS
`CGT
`ARG
`CGT
`CGT
`ARG
`CGT
`ARG
`
`96
`97
`98
`99
`100
`101
`102
`103
`103
`105
`106
`107
`103
`
`TYR
`THR
`PHE
`GLY
`GLN
`GLY
`THR
`LYS
`LEU
`GLU
`ILE
`LYS
`AHG
`
`Genzyme Ex. 1026, pg 722
`
`

`
`SEQUENCES OF
`
`PROTEINS OF
`
`IMMUNOLOGICAL
`INTEREST ,
`
`Tabulation and Analysis of
`Amino Acid and Nucleic Acid Sequences of
`Precursors, V-Regions, C-Regions, J-Chain,
`
`l32—Microglobulins, Major Histocompatibility Antigens,
`Thy-1, Complement, C-Reactive Protein, Thymopoietin,
`Post-gamma Globuiin, and a2-Macroglobulin
`
`1 983
`
`Elvin A. Kabat*, Tai Te wuT , Howard Bilofsky i,
`Margaret Reid-Mi/Ieri, and Harold Perryi
`
`*Depts. of Microbiology, Human Genetics and Development, and Neurology, Cancer Centerl
`Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New
`York, NY 10032 and the National Institute of Allergy and Infectious Diseases, Bethesda, MD
`20205
`
`TDepts. of Biochemistry, Molecular Biology, and Cell Biology, and Engineering Sciences and
`Applied Mathematics, Northwestern University, Evanston, IL 60201 and the Cancer Center,
`Northwestern University Medical School, Chicago, IL 60611
`1 Bolt Beranek and Newman lnc., Cambridge, MA 02238
`
`The collection and maintenance of this data base is sponsored through Contract N01-RR-8-2118
`by the following components of the National Institutes of Health, Bethesda, MD 20205:
`Division of Research Resources
`National Cancer Institute
`National Institute of Allergy and Infectious Diseases
`National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases
`National Institute of General Medical Sciences
`
`U.S. DEPARTMENT OF HEALTH
`AND HUMAN SERVICES
`Public Health Service
`National Institutes of Health
`
`(1933)
`
`It-u.r. HEALTH SCIENCES LIBRARY
`._-.....-.1-..-.-rmv nu nnr Tu-nD1u'rA
`TMVTR
`
`Genzyme Ex. 1026, pg 723
`
`Genzyme Ex. 1026, pg 723
`
`

`
`Our listing of sequences will be kept up to date. Investigators are invited to send additional
`sequence data when accepted for publication. Send two copies of the manuscript together with
`a letter of acceptance from a journal to:
`Dr. E.A. Kabat
`National lnstitutes of Health
`Building 4, Room 337
`9000 Rockville Pike
`Bethesda, Maryland 20205
`
`If a computer tape is available, please send it to facilitate entering sequences.
`when published, three reprints should be provided.
`
`If any published sequences have been overlooked or if any errors are found, please bring them to
`our attention.
`"
`
`Genzyme Ex. 1026, pg 724
`
`Genzyme Ex. 1026, pg 724
`
`

`
`
`
`246
`
`11
`T1
`
`12
`L6
`
`13
`T2
`
`14
`L7
`
`'
`;
`:
`
`.
`if
`I
`"
`'
`,|
`.
`
`___
`AAT
`GTG
`GTT
`AGT
`TAT
`GTT
`To
`TGG
`TAT
`CAA
`GAG
`AAA
`CCA
`GAG
`GAA
`TCT
`CCT
`AAA
`
`
`
`
`
`ATT
`AGT
`AGG
`TGG
`TTG
`Gcc
`TGG
`TAT
`CAG
`CA5
`AAA
`CCA
`GGG
`AAA
`GCC
`CCT
`AAG
`
`ATT
`GGT
`AGT
`AGO
`TTA
`AAC
`TGG
`CTT
`CAG
`CAG
`GAA
`CCA
`GAT
`GGA
`ACT
`ATT
`AAA
`
`
`
`
`
`__,.
`TTC
`GGA
`GGG
`GGG
`ACC
`AAG
`CTG
`GAA
`ATA
`———
`AAA
`CGG
`
`— —
`— —
`- -
`— -
`— —
`— ~
`— —
`— _
`— —
`— —
`~**
`___
`
`TTG
`GGT
`
`ACC
`AAG
`CTG
`GAA
`ATC
`———
`AAA
`CGT
`
`TTc
`GGA
`
`ACC
`AAG
`CTG
`GAA
`ATA
`———
`AAA
`___
`
`___
`
`TTc
`GGc
`GGG
`TcG
`ACA
`AAG‘
`TTG
`GAA
`ATA
`___
`AAA
`CGT
`
`___
`
`___
`
`___
`
`___
`
`
`
`___
`TTG
`GGT
`GGA
`GGA
`ACC
`AAA
`CTG
`ACT
`GTC
`CTA
`GGT
`___
`
`___
`TTc
`GGT
`GGT
`GGA
`ACC
`AAA
`GTG
`AcT
`GTC
`CTA
`GGC
`___
`
`___
`
`___
`
`___
`
`___
`
`Genzyme Ex. 1026, pg 725
`
`CODONS OF VARIABLE REGION LIGHT CHAINS
`21
`20
`19
`13
`‘I7
`16
`15
`10
`9
`8
`7
`G
`1
`2
`3
`4
`5
`$43 MOPC PLAI
`ueaoa
`|G13
`IG99
`PKAFPA
`HK101 HK102 MOPC MOPC MOPC MDPC K2A MPI3 MOFO s1o7
`315
`-13
`LAMBDA LAMBDA LAMBDA
`(11)24
`41
`21
`1e7
`2'?‘
`11
`1735
`El
`___
`___
`___
`___
`___
`GGA
`—__
`——_
`_——
`_——
`_—— _——
`.._~
`___ ___ ___ ___ ___
`GCT GCT
`GCT
`GCT
`GCT
`ATT
`ATC
`ATT
`ATT
`ATC ATC ATT ATC AAT ATC ATC ATC ATC
`GAG GAG
`cAG
`GAG
`GAG
`GAG
`GAG
`AAC
`GAT
`GAG GAG GAG GAc GAA GAG GAG GAG GAc
`GTT GTT
`GTT
`GTT
`GTT
`CA6
`GTA
`GTG
`CAG CAG GTG CAG GTG AAG AAG TTG TTG GTG
`GTG GTG
`GTG
`GTG
`GTG
`ATG
`ATG
`ATA
`ATG ATG CTG ATG CTC ATG ATG CTG CTG
`ATG
`ACT
`ACT
`ACT
`ACT
`ACT
`ACC
`ACC
`ACC
`ACT ACT ACC ACC
`ACO ACC ACC ACT ACT
`ACO
`cAG CA6
`cAG
`cAG
`GAG
`cm; on GAG
`cAG GAG cAA cAG GAG CAG GAG GAG me me
`GAA GAA
`GAA
`GAA
`GAA
`TcT
`TcT
`GAT
`TCT TCT TcT TcT TCT TcT TCT TcT TcT
`TCT
`TCT TCT TcT
`TcT
`TcT
`TOT
`CCA
`ccc
`GAA
`ccA ccA ccG CCA GGA ccA ccA GOA ccA GAG
`GCA GCA GGA
`GGA
`GCA
`GCA
`TCC
`AAA
`CTC
`GCC GCC GCT
`TCC GCA TCT TCT GCC GCC
`AAA
`_——
`———
`-.— ———
`———
`———
`TCC
`TCC
`TCC
`TCC
`TCT
`TCC ATA TCC TCC ATC ATC
`TTC
`CTC
`CTC
`CTC
`CTC CTC CTC
`TTA
`ATG
`AAT
`CTA
`TTG TTA ATG ATG ATG CTG CTG
`ATG
`ACC
`ACC
`ACC
`ADC ACC ACC
`TCT
`TCC
`CCT
`TCT
`GOT
`TCT GCT TAT TAT TCT TCT
`TCC
`AcA
`AGA
`AcA
`AGA ACA AcA
`GCC
`ATG
`GTG
`GGA
`GTG Gcc Gcc GcA GGA GTG GTG
`AGA
`TCA
`TCA
`TCA
`TCA TCA TCA
`TCT
`TCA
`ACT
`TCT
`TCT TCT
`TCT TCT TCT AGT AGT
`TCA
`ccT
`ccT
`ccT
`ccT CCT
`ccT
`GTG
`GTA
`TcT
`GTG
`CTA GTG CTG GTA GTA ccA ccA GTA
`GGT
`GGT
`GGT
`GGT GGT GGT
`GGA
`GGA
`GGA
`GGT
`GGG GGA GGG GGA GGA GGA GGA
`GGA
`GAA
`GGA
`GAA
`GAA GGA GAA
`GAA
`GAG
`GAA
`GAA
`cAG GAA cAG GAG GAG GAA GAA
`GAO
`AGA
`AGA
`AGA
`AGA AGA AGA
`AGA
`AGG
`TAC
`AcT
`AGG AGA
`AAG AGA AGA AGA AGA
`AGG
`GTC
`GTG
`GTG
`GTG GTG GTG
`GTG
`GTC
`GTT
`GTC
`Gcc GTC GTG GTO GTG GTG GTC
`GTC
`ACA
`ATA
`AGA
`ACA ATA AGA
`AGT Acc
`Tcc
`ACC
`Acc
`ACT ACT AGT AGT
`AGc
`cm
`on
`CTC
`cTc CTC cTc
`cTc
`TTG
`ATC
`ATc
`ATA
`ATc ATG TTc TTc
`ATc
`AcT
`AGT
`ACT
`AcT ACT ACT
`AGT
`TcG
`AGA
`Tcc
`TcT ACT Tcc TCC Acc
`TGT
`TGT
`TGT
`TGT
`TGT
`TGT
`TGT
`TGC
`TGT
`TGc
`TGC TGc TGc TGc
`TGc
`cGc
`GGC
`CGC
`[266
`cm:
`cGc
`CGG
`GGA
`AGA
`AAG AAG AGG AGG
`AAG
`TcA
`TcA
`TcA
`TcA TGA TCA
`GGA
`Gcc
`GOG GGG Gcc Gcc Gcc
`AGT
`AGT
`ACT
`AGT
`AGT
`AGT
`AGT
`AGT AGT AGT AGT
`AGT
`———
`---
`---
`---
`---
`__-
`A T
`--- --- ——— ___
`ACT
`ACT
`ACT
`ACT ACT
`ACT
`GeA
`CAG CAG CAG CAG
`___
`___
`___
`___
`___
`___
`GTT
`___ ___ ___ ___
`___
`___
`___
`___
`___
`_-_
`GAT
`GGG
`GGG
`GGG
`GGG GGG
`GCT
`GcT
`GGT
`GcT GCT
`GTT
`GTT
`GTT
`GTT GTT
`___
`ACA
`ACA
`ACA
`ACA AGA
`GAC GAG AGO AGC
`TAT
`AcT
`ACT
`ACT
`AcT ACT
`ATT ATT ATT ATT
`Gec
`AGT
`AGT
`GGT
`AGT
`AGT
`AAT AAT GGC GGC
`AAT
`AAG
`AAC
`AAC
`AAc AAC
`AGG AGc ACA AGA
`AGT
`TAT
`TAT
`TAT
`TAT
`TAT
`ACT
`TAT TAT AGC AGC
`TTT
`Gcc
`Gcc
`GCC
`Gcc Gcc
`GTG
`TTA TTA ATA ATA
`ATG
`AAC
`AAC
`AAG
`AAC AM)
`Acc AGc cAc CAO Gcc
`me
`TGG
`TGG
`TGG
`TGG TGG
`TGG TGG TGG TGG
`TGG
`TGG
`GTC
`GTT
`GTG
`GTG ATC
`TTc TTC TAT TAT
`TAT
`TAT
`TAT TAO
`CAA
`CAA
`CAA
`CAA CAA
`CAG CAG CAG CAG
`CAG
`CAG CAG CAG
`GAA
`GAA
`GAA
`GAA
`CAG CAG CAA CAA
`CAG
`CAG CAG CAG
`AAA
`AAA
`AAA
`AAA
`AAA AAA AGA AGA
`AAA
`AAA AAA AAA
`CCA
`CCA
`CCA
`CCA
`CCA CCA ACA ACA
`CCA
`C-AG CAG CCA
`GAT
`GAT
`GAT
`GAT
`GGG GGG AAT AAT
`GGG
`GGA GGA GGA
`cm
`cAT
`CAT
`CAT
`AAG AAA GGT GGT
`cAA
`AAA AAA cAG
`TTA
`TTA
`TTA
`TTA
`TCT TCT TCT TCT
`TCT
`TCT
`TCT CCA
`TTC
`TTC
`TTC
`TTC
`CCT CCT CCA CCA
`CCT
`CCT CCT CCC
`ACT
`ACT
`ACT
`ACT
`AAG AAG AGG AGG
`AAA
`GAG CAG AAA
`GGT
`GGT
`GGT
`GGT
`Acc Acc GTT cTT
`GTA
`cm CTC CTC
`cTA
`CTA
`GTA on
`cTG cTG cTc cTc
`GTG
`cm cTG cTc
`ATA
`ATA
`ATA
`ATA
`cTc ATc ATA ATA
`ATT
`GTG GTc ATC
`GGT
`GGT
`GGT
`GGT
`TAT TAT AAG AAG
`TAT
`TAT
`TAT TAT
`GGT
`GGT
`GGT
`GGT
`CGT CGT AAT TAT
`TCG
`AAT AAT CGT
`Acc
`Acc
`Acc
`Acc
`GGA GGA GcT GCT
`GGA
`GGA GOA GOA
`AAC
`AGO
`AAC
`AAC
`AAC AAC TCT TGT
`TCC
`AAA AAA TCC
`AAC
`AAC
`AAC
`AAC
`AGA AGA GAG GAG
`TAG
`ACC ACC AAC
`cGA
`GGA
`CGA
`cm
`TTG TTG TCC TcT
`cGG
`TTA TTA CTA
`GcT
`GOT
`GcT
`GGT
`GTA GTA ATC ATC
`TAO
`GGA GGA GAA
`ccA
`GGA
`.ccA
`CCA
`GAT GAT TcT TcT
`ACT
`GAT GAT TCT
`GGT
`GGT
`GGT
`GGT
`GGT GGT GGG GGT TcT GGG GGG GGG GGG
`GGA
`GTT
`GTT
`GTT
`GTT
`GTG GTG ATC GTc GGA GTc GTO ATc ATc
`GTG
`ccT
`ccT
`ccT
`ccT
`cm ccA ccT ccA GTC ccA ccA ccT ccT ccc
`Gcc
`GTG
`GCC
`Gcc
`TcA TcA Gcc
`AAA
`cc». TCA TcA Tcc Tcc
`GAT
`AGA
`AGA
`AGA
`AGA
`AGG AGG AGG AGG
`GCT AGG AGG AGG AGG
`cGc
`TTc
`TTC
`TTc
`TTc
`TTC TTC TTC TTC cec TTc TTc TTT TTT
`TTc
`TCA
`TCA
`TCA
`TCA
`AGT AGT AGT
`AGT
`TTC AGT AGT AGT AGT
`AC
`GGC
`Get:
`(366
`GGc
`(360 G613 GGC GGC
`AGT GGC GGG GGC GGC
`GGC
`Tcc
`Tcc
`Tcc
`Tcc
`AGT AGT AGT
`AGT GGc AGT AGT AGT AGT
`AGT
`GTG
`GTG
`cTG
`GTG
`GGA GGA GGG AGG
`AGT GGA GGA GGA GGA
`GGA
`A17
`A71’
`ATT
`ATT
`TcA TcA TcT 'rcT GGG TcT TCT TCA TCA
`TCT
`GGA
`GGA
`GGA
`GGA
`GGA GGA AGG GGG TcT GGG GGG GGG GGG
`GGG
`GAC
`GAO
`GAG
`GAG
`AcA AGA AcA TcA GGG CAA cAA AGA AGA
`ACG
`AAG
`AAG
`AAG
`AAG
`cAA cAA
`GAT Acc GAT GAT GAT GAT
`GAT
`GcT
`GcT
`GcT
`GGT
`TAT
`TAT
`TAT TcT TTT TAT TTT TTT
`TTc
`Gcc
`Gcc
`Gcc
`GCC
`TCT TcT
`TcT TAc TcT TcT AcT AGT
`ACT
`CTC
`CTC
`CTC
`CTC
`CTC CTC CTC CTC
`TCT CTC C-TC COT CTT
`TTC
`ATC
`ATC
`ATC
`ATC
`AGA ATC ATC ATC ATC
`ATC
`Acc
`Acc
`Acc
`Acc
`GT0 Acc Acc AGC AGE
`ACC
`GGA
`GOA
`GcA
`AGA
`AGc cTG cTG GTG GTG
`GTG
`383
`383
`883
`£83
`138 A83 233 23? A3? A5?
`CAG
`CAG
`cAG
`GAG
`GTG GAG GAG GAG GAG
`CAG
`ACT
`ACT
`ACT
`ACT
`GAG TAT TAT TCT TCT
`GCT
`GAG
`GAG
`GAG
`GAG
`GcT GAA GAA GAA GAA
`GAA
`GAT
`GAT
`GAT
`GAT
`GAA GAT GAT GAT GAT
`GAc
`GAG
`GAT
`GAG
`GAG
`GAT ATG ATG ATT ATT
`CTA
`GGA
`GGA
`GOA
`GGA
`GAT GGA GGA GGA GGA
`GGA
`T
`TA
`‘A334
`TIT H‘
`"5
`H?
`#1?
`TAT
`TAC
`TTc
`TTc
`TTc
`TTC
`TAC
`TGT
`TGT
`TGT
`TGT
`TGT
`TGC
`GAG
`GOT
`GCT
`GcT
`GcT
`GAG
`CAA
`GTA
`cTA
`CTA
`CTA
`CAG
`CAT
`TGG
`TGG
`TGG
`AGT
`AGT
`mo
`TGG
`TAT
`TAC
`GGT
`ACT
`AAC
`TAC
`ccT
`GAT
`CCA
`CCC
`TGG
`___
`Ace
`GTG
`,__
`~—~
`
`—_»
`ATC
`GAC
`CA6
`ATS
`ACC
`CAG
`TcT
`CCA
`TCC
`TCA
`CTG
`TCT
`GCA
`TGT
`GTA
`GGA
`GAG
`AGA
`GTG
`Acc
`ATC
`ACT
`TGT
`cGG
`GCG
`AGG
`
`
`
`ATT
`AGO
`AGc
`TGG
`TTA
`Gcc
`TGG
`TAT
`CAG
`CAG
`AAA
`CCA
`GAG
`AAA
`GCC
`CCT
`AAG
`
`o
`2
`1
`3
`4
`5
`a
`7
`3
`9
`10
`T1
`12
`13
`1-1
`:5
`15
`17
`13
`15
`2n
`21
`a2
`23
`24
`25
`ZS
`§;A
`27B
`270
`270
`27E
`27F
`23
`29
`so
`31
`32
`as
`34
`as
`as
`37
`35
`as
`40
`41
`42
`43
`44
`45
`45
`47
`At:
`as
`50
`51
`52
`53
`54
`55
`56
`57
`55
`55
`so
`s1
`62
`53
`64
`as
`as
`67
`as
`65
`70
`71
`72
`73
`7;
`73
`3?
`79
`80
`31
`a2
`H3
`54
`33
`a7
`as
`as
`so
`91
`gg
`94
`95
`95
`97
`97A
`97B97C
`911)97E
`97F
`98
`99
`13(1)
`102
`103
`104
`105
`‘KW
`ICBA
`‘I07
`108
`
`F
`R
`'
`
`C
`D
`H
`1
`
`F
`n
`2
`
`C
`D
`R
`2
`
`F
`H
`3
`
`3
`R
`3
`
`F
`R
`4
`
`
`
`
`
`Genzyme Ex. 1026, pg 725
`
`

`
`247
`
`SOURCE: CODONS OF VARIABLE REGION LIGHT CHAINS
`1) HD1101: HUMAN FDETAL LIVER
`2) HK102: HUMAN FOETAL LIVER
`3) MOPC41: MOUSE ADULT
`4) l0Pc21: MOUSE MRNA FROM MOFO21
`5) MOPC1l7: MOUSE ADULT
`E) Il0Pc149(I(2): MOUSE EMBRYO & PLASMOCYTOMA
`7) I(2A: MOUSE EMBRYO
`B) IPC11: MOUSE PLASMACYTDMA
`9) HOFCTISBZ MOUSE FLASMACVTOMA
`10) 51075: MOUSE EMBRYO
`11) T1: MOUSE ADULT
`12) L6: MOUSE ADULT LIVER
`15) T2: MOUSE ADULT, ABNORMAL J OMITTED
`14) L7: MOUSE ADULT LIVER
`15) FKAPPA(11)2I: MOUSE MRNA FHCM MPCI1
`15)
`IGQBLAHBDA: MOUSE EMBRVO
`17) IG13LAMIDA: MOUSE EMBRYO
`1B)
`IGSOSLAHBDA: MOUSE ADULT
`19) S43: MOUSE ADULT
`20) MOPG315: MOUSE ADULT
`21) PLAI-13: MOUSE ADULT
`REFERENCE: CODONS DF VARIABLE REGION LIGHT CHAINS
`1) HK101: BENTLEY,D.L. A HABB|TTS,T.H. (1980) NATURE.2B8.73D-733.
`2) HK102: BENTLEY, L A HABBl1'l'S,T.H. (1980) NATURE,28B.730-733.
`S) HDPC41:
`SE|DMAN..LG..MAX,E.E.
`Eu LEDEFLP. (1979) NATURE,IEl.'I,37CI—375.
`4) IIOPCZI:
`FONG.K.LEl,A. E SALSEFLW. (1979) BIOCHEM.BIOFHVS.FIES.COMMUN..9U.832-B41: HAMLYN.P.H.,GA|T,M.J.
`FlES.,Q.4435-4494.
`5)
`lAOPc15T:
`JOHCI,R..WE|5SMAN,1.L.EAFILY.F..COLE,J. a. HOOD.L. (1950) FHOC.NAT.ACAD.SCI.USA.77.1106-1110.
`8)
`lAOPC149(I(2): NISHIOKA.Y. E LEDERP. (1930) J. BIOL.CHEM..255,3S91-3694.
`7) KZA: NISHIOKA,Y. & LEDEFLF‘. (1980) J.BIOL.CHEM:,255,3591-5594.
`B) MPC11:
`SE|DMAN.J.G. E LEDER,P. (1980) NATURE,266,779-753.
`9)
`llOPC1T3B: MAX.E.E.,SEIDMAN.J.G.,MlLLER,H. 8. LEDEH,P. (1980) CELL.21.799-799.
`
`1 D) 51073: KWAN,
`MAX.E.E..SElDMAN,J.G.,LEDEFLP. A SCHARFF.M.D. (1981) CELL,2fi,57-66.
`11) T1: ALTENBUFIGER,W.,STEINMETZ,M_ E ZAGHAU,H.G. (1950) NATUHE,2fl7,&33r8ll7_.
`PECH,M.,HOCHTL .SCHNELLH. 8- ZACI-IAU.H.G.
`(1981) NATUFIE.29‘|.66B-670.
`12) L6:
`13] T2: ALTENBUHGER,W STEINMEI'Z.M. 8. ZACHAU.H.G. (1980) NATURE.2B7,60G-607.
`14) L1:
`PECH,M.,HOCHTL,
`..SCHNELL.H.
`En ZACHAU.H.G. (1981) NATURE.291,66G-670.
`15) PKAPFA(11)2l:
`FIABBITTS,T.H.,HAMLYN,P.H. L MA1'I’HYSSEN5.G. (1950) CAN.J.E|OCHEM.,58,176-187.
`16) IGIBLAIIDA: BERNARD,
`0ZUMI.N. & TONEGAWA.S. (1975) CELL,15.1IS3-1144.
`17) IG13LAMIDA: TONEGAWA.S..MAXAM,A.M.,T|ZAHD.R..EERNARD,O. L G|LBERT.W. (1975) PROC.NAT.ACAD.SCl.USA,75.1485-1439.
`13) IBIISLAHIIIA:
`EEFINAFID,D‘,HDZUMI,N. A TONEGAWA3. (1978) CELL,15,1133-1144.
`19) S43: BOTHWELLA.LM.,PASKlND,M..RETH.M.,IMANISHI-KARl,T..RA.JEWSKY,K. A BALTlMDRE,D. (I982) NATURE,29E,380-382.
`20) IAOPC31§: BOTHWELL.A.LM..PASKlND,M.,RETH.M.,IMANISHI-KAFI|.T..RAJEWSKY.K. & BALTlMOHE.D. (1962) NATURE.298.3B0-382.
`21) PLAI-13: EOTHWELL.A.L.M.,PASK|ND.M.SCHWARTZ.R.C..SONENSHEIN.G.E..GEF|'ER,M.L & BALTIMDRED. (1981) NATURE,290.656T.
`
`I} MlLS‘[ElN,C. (1991) NUCLACIDS
`
`
`
`,=__....4_,1....
`
`Genzyme Ex. 1026, pg 726
`
`Genzyme Ex. 1026, pg 726
`
`

`
`24$
`
`5
`s21
`-HL
`
`5
`H11
`
`CODONS OF VARIABLE REGION HEAVY CHAINS
`1
`2
`3
`4
`LAMBDA LAMBDA LAMBDA HG3
`vr-125
`v1-152
`VH32
`-..
`GAG
`GTG
`GAG
`GTG
`TTG
`GAG
`TCT
`GGG
`GGA
`GGG
`TTG
`GTA
`GAG
`GGT
`GGG
`GGG
`TGG
`CTG
`AGA
`GTG
`TGG
`TGT
`GGA
`GGG
`TGT
`GGA
`TTC
`AGG
`TTT
`AGG
`AGG
`TAT
`GGG
`ATG
`AGG
`———
`—-—
`TGG
`GTG
`GGG
`GAG
`GGT
`GGA
`GGG
`AAG
`GGG
`GTG
`GAG
`TGG
`GTG
`TGA
`GGT
`ATT
`AGT
`GGT
`__
`---
`AGT
`GGT
`GGT
`AGG
`AGA
`TAG
`TAG
`GGA
`GAG
`TCC
`GTG
`AAG
`GGG
`GGG
`TTG
`AGG
`ATG
`TGA
`
`O
`1
`2
`a
`4
`5
`s
`7
`s
`9
`1o
`11
`12
`13
`14
`15
`1s
`17
`113
`19
`2o
`21
`22
`2:;
`24
`25
`29
`27
`2a
`29
`an
`31
`32
`33
`34
`35
`35A
`353
`aa
`37
`ea
`39
`4o
`41
`42
`43
`44
`45
`4s
`47
`4s
`49
`so
`51
`52
`52A
`523
`52c
`sa
`54
`55
`53
`57
`5:
`59
`so
`e1
`e2
`sa
`54
`s5
`as
`s7
`as
`s9
`711
`71
`72
`73
`74
`75
`76
`77
`78
`79
`so
`111
`B2
`a2A
`525
`a2G
`G3
`a4
`as
`as
`a7
`as
`a9
`9o
`91
`92
`93
`94
`95
`9s
`—-—
`97
`___
`es
`___
`99
`___
`11:0
`___
`1ooA
`___
`1003
`___
`1ooG
`___
`100D
`_ -
`1ODE
`..
`100F
`1ooG W.
`1001-1 N,
`1001
`___
`1oou
`___
`1oo1<
`___
`101
`102
`103
`104
`105
`106
`107
`109
`109
`110
`111
`112
`
`TAT
`GTT
`GAA
`ATG
`AAG
`AGG
`GTG
`AGA
`GGG
`GAG
`GAG
`ATG
`GGT
`GTG
`TAT
`TAG
`TGT
`
`
`
`
`
`———
`—-—
`___
`
`F
`R
`1
`
`C
`3
`1
`
`E
`2
`
`3
`R
`2
`
`,,
`R
`3
`
`C
`D
`H
`3
`
`R
`4
`
`12
`11
`10
`9
`3
`7
`MOPC
`MCPC MOPC HPG MOPC
`s
`141
`soa
`157
`75
`141
`107
`ADULT EMBRYO
`___
`_-_
`-__
`___
`___
`___
`.1._
`GAG
`GAG
`GAG GAG GAA GAG GAG
`GAG
`GTG GTG GTG GTG GTG
`GTG
`GTA
`GTA
`GAG GAG ATG AAG AAG
`AAG
`GAG
`GAG
`GTG GTG GTG GTG GTG
`GTG
`GTG
`GTG
`GTG GTG GTG GTG GTG
`GTG
`AAG
`AAG
`GAG GAG GAG GAA GAA
`GAA GAG GAG
`GAG
`TGT TGG TGT TGT
`TGT
`TGT
`TGA
`TGA
`TGT
`GGG GGG GGG GGA GGA
`GGA GGA
`GGA
`GGA
`GGT GGA GGA GGA GGA
`GGA GGA
`GGT
`GGT
`GAG GGG GGG GGG
`GGG
`GGG GGG
`GGG
`GGG
`GTG TTA TTA TTG TTG
`TTG‘ TTG GTG
`GTG
`AAG GTT GTG GTA GTA
`GTA GTG
`GTG
`GTG
`AAG GAG AAG GAG GAG
`GAG GAA
`GGG
`GGG
`GGT GGT GGT GGT GGT
`GGT GGT
`GGG
`GGG
`GGG GGG GGA GGG GGG
`GGG GGA
`TGA
`TGA
`GGG GGG GGG GGT GGT
`GGT GGA
`GAG
`GAG
`TGA TGG TGG TGT
`TGT
`TGT
`TGG
`AGG
`AGG
`GTG GTG GTG GTG GTG
`GTG ATG GTG
`GTG
`AAG AGA AAA AGA AGA
`AGA AAA
`TGG
`TGG
`GTT GTG GTG GTG GTG
`GTG GTG
`ATG
`ATG
`TGG TGG TGG TGG TGG
`TGG TGG AGA
`AGA
`TGG TGT TGT TGT
`TGT
`TGT
`TGT
`TGG
`TGG
`AAG GGA GGA GGA GGA
`GGA GTT
`AGG
`AGG
`GGA GGG GGG ACT
`AGT
`ACT GGG GTC
`GTG
`TGT TGT TGT TGT
`TGT
`TGT TGT
`TGA
`TGA
`GGA GGA GGA GGG GGG
`GGG GGA
`GGA
`GGG
`TAG TTG TTC TTG TTG
`TTG TTG TTG
`TTG
`AGG AGG AGT AGG
`AGG
`AGG AGT
`TGA
`TGA
`TTG TTG TTG TTG TTG
`TTG TTG TTA
`TTA
`AAG AGT AGT AGT
`AGT
`AGT AGT
`AGG
`AGG
`AGG AGG AGG GAT GAT
`GAT AAG
`GGG
`GGG
`TAG TAG TAT TTG TTG
`TTG TAG TAT
`TAT
`TAT TGG GGG TAG TAG
`TAG TGG GGT
`GGT
`ATG ATG ATG ATG ATG
`ATG ATG GTG
`GTA
`GAG GAG TGT GAG GAG
`GAG AAG
`AAG
`AAG
`___ ___ _
`___
`___
`___
`___
`___
`___
`___ '-
`—-—
`—-— —-—
`—-—
`-.—
`TGG TGG TGG
`TGG
`TGG TGG TGG
`TGG
`
`GTG GTG GTT GTG GTG
`GTG GTG GTT
`GTT
`GGA GGG GGG GGG GGG
`GGG GGG
`GGG
`GGG
`GAG GAG
`GAG GAA
`GAG GAG
`GAG
`GAG
`GGG GGT
`GGT CCT
`AGT TGT
`GGT
`GGT
`GGT GGA
`GGA GGA
`GGA GGA
`GGA
`GGA
`GGA GGG
`GGG GGG
`GGG GAG
`GGA
`GGA
`GAA AAG
`AAG AAG
`AAG AAG
`AAG
`AAG
`GGG GGG
`AGA AGA
`AGA GGG GGT
`GGT
`GTT GTG
`GTG GTG
`GTG CTT GTG
`GTG
`GAG GTG
`GAG GAG
`GAG GAG GAG
`GAG
`TGG TGG
`TGG TGG
`TGG TGG TGG
`TGG
`ATG GTG
`ATT ATT
`ATT GTT
`GTG
`GTG
`GGA TGA
`GGT GGT
`GGT GGT
`GGA
`GGA
`ATA GGT
`GGA GGA
`GGA GAA AGG
`ATG
`ATG ATT
`AGT AGT
`AGT ATT
`ATA
`ATA
`AAG AAT
`AGA AGA
`AGA AGA
`TGG
`TGG
`GGT AGT
`AAG AAG
`AGT TTG
`___ ___
`AAA AAG
`AAA AAA
`
`___
`___
`_-. __,
`GGT GGT
`GGT
`___
`GGT
`GGT
`AGT GAT
`AAT AAT
`GAT
`TGT
`AAT
`GAT
`GGT GGG
`GAT AAA
`GAT GGT
`GGA
`GGA
`GGT AGT
`TAT
`TAT
`TAT
`TAT
`AGG AGG
`AGA AGA
`AGA GGA AGG
`AGG
`AGA AGA
`AGA AGA
`AGA AGA
`AGA
`AGA
`AGG AGG
`GAG GAA
`GAA GAT
`GAG
`GAG
`TAG TAG
`TAG TAG
`TAG TAT
`TAT
`TAT
`GGA GGG
`AGT AGT
`AGT GGG AAT
`AAT
`GAG GAG
`GGA GGA
`GGA GAG
`TGA
`TGA
`AAG TGG
`TGT
`TGT
`TGT
`TGT
`AGT
`GGT
`TTG GTG
`GTG GTG
`GTG GTG GTG
`GTG
`GAG AAG
`AAG AAG
`AAG AAA
`AAA
`AAA
`GGG GGG
`GGT GGT
`GGT GGG TGG
`TGG
`AGA GGA
`GGG GGG
`GGG AGG
`AGA
`AGA
`GTG TTC
`TTC TTG
`TTG TTG
`GTG
`GTG
`AGG AGG
`ATG ATG
`ATG AGG
`AGG
`AGG
`ATG ATG
`GTC GTC
`GTG ATG
`ATG
`ATG
`AGG TGG
`TGG TGG
`TGG TGA AGG
`AGG
`AGG AGA
`AGA AGA
`AGA AGA
`AAG
`AAG
`GAG GAG
`GAG GAG
`GAG GAT
`GAG
`GAG
`AGG AAG
`AGT AGT
`AGA GAT
`AAG
`AAG
`TGG GGG
`TCO TGG
`TGG TGG
`TGG
`TGG
`AGG AAG
`GAA GAA
`CAA AAA
`AAG
`AAG
`AGG AAG
`AGG AGG
`AGT AGT
`AGG
`AGG
`AGA AGG
`ATG ATG
`GTG AGT
`GAA
`GAA
`GTG GTG
`GTG GTG
`GTG GGT GTT
`GTT
`TAG TAT
`TAG TAG
`TAG TAG TTC
`TTG
`ATG GTG
`GTT CTT
`GTT GTG TTA
`TTA
`GAG GAA AAA
`AAA
`ATG ATG ATG
`ATG
`AAT AAG
`AAG
`AAG
`GGG AAG
`AGT
`AGT
`GTG TTA GTG
`AGA
`GAA
`GGT
`AGT
`GAG
`GAT
`GAG
`GAG
`AGT
`AGA
`GGG
`GGG
`AGT
`AGG
`TAT
`TAG
`TAG
`TAG
`TGT
`TGT
`AGA
`GGG
`AGA
`AGG
`GAT
`GTG
`GGG
`TGA
`GAG
`ATT
`TAG
`TAT
`GGT
`TAG
`AAT
`TAG
`AGG _——
`GGT
`-__
`,,,
`GGT
`TAG G_-
`AGG
`TTT
`___
`GAG
`GGG
`.,-
`AAA
`TAG ___
`TAG
`TTG ___
`TTG
`___
`___
`AGT
`___
`TTG
`___
`___
`___
`_,_
`___
`GAG
`GAG
`GAT
`GTG TAG TAG
`TGG TGG TGG
`GGG GGG GGT
`GGA GAA GAA
`GGG GGG
`GGA
`AGG AGG
`AGG
`AGG AGT
`TGA
`GTG GTG GTG
`AGG AGA
`AGT
`GTG GTG GTG
`TGG TGG TGG
`TCA TCA TCA
`
`
`TCA TCA TCA TCA
`
`
`
`___
`
`
`___
`
`—-—
`
`19
`23
`
`20
`a-
`
`21
`102
`
`22
`IE
`17
`16
`15
`14
`I3
`PCH
`.1 MP0 31-3 S43 MOPC 1115
`105
`55a
`11
`21
`-1
`-—— _—— ——— ———
`.--
`._r
`___ ___
`GAG GAG GAG GAT GAG GAG GAG GAT GAG
`GTG GTG GTG GTG GTG GTC GTG GTG GTG
`GAG GAA GAA GAG GAA GAA GAA GAA GAG
`GTG GTG GTA GTG GTG GTG GTG GTG GTG
`GAG GAG GAG GTG GAG GAG GAG GAG GAG
`GAG GAG GAG GAG GAG GAG GAG GAG GAA
`TGT GGT GGT TGT GGT GGT CCT GGT TGT
`GGA GGG GGG GGG GGG GGG GGG GGG GGA
`GGT GGT GGT GGA GGT AGT GGT GGT GGT
`GAG GAG GAG GGG GAG GAA GAG GAG GAG
`GTG GTT TTT
`TTA GTT GTG GTG GTG GTG
`GTA GTG GTG GTG GTG GTG GTG GTG GTG
`AGA AAG AAG GAG AAG AAG AGG AAG AAG
`GGT CCT GGT GGT GGT GGT GGT GGT GGT
`GGG GGG GGG GGA GGG GGG GGG GGG GGG
`AGT GGT GGT GGG GGT GGT TCT GGT GGT
`TGA TGA TGA TGG TGA TCA TGA TGA TGA
`GTG GTG GTG GGG GTG GTG GTG GTG GTG
`AAG AAG AAG AAA AAG AAG AAG AAG AAG
`ATG CTG GTG GTG GTG GTG GTG GTG ATA
`TGG TGG TGG Tcc
`TGG TGG TGG TGG TGG
`TGG TGG TGG TGT
`TGG TGG TGG TGG TGG
`AAG AAG AAG GGA AAG AAG AAG AAG AAG
`GGT GCT GCT GGG GGT GGT GGT GGT GGT
`GGT TGT TGT
`TGT
`TGT TGT TGT TGT TGT
`GGA GGG GGG GGA GGG GGG GGG GGG GGG
`TAG TAG TAG TTG TAG TAG TAG TAG TAT
`AGG AGG AGG AGT
`AGG AGG AGG AGG AGG
`TTC TTG TTG TTG TTC TTC TTG TTC TTC
`AGT AGG AGG AGT
`AGG AGG ACC Acc AGA
`AAG AGG AGG AGG AGG AGG AGG AGG AGG
`TAG TAG TAG TTT
`TAG TAG TAG TAG TAG
`TGG TGG TTA GGA TGG TGG TGG TGG TAT
`ATA ATG ATG ATG ATG ATG ATG ATG ATA
`GGT GAG GAG GAG GAG GAG GAT GAG GAG
`___ ___ --_
`._V 2” WA ___ -.- ___
`——— ——— WA —-A A-- —-— —-— —-— —-—
`TGG TGG TGG TGG TGG TGG TGG TGG TGG
`GTA GTG GTG GTT GTG GTG GTG GTG GTG
`AAG AAG AAG GGT AAG AAG AAG AAG AAG
`GAG GAG GAG GAG GAG GAG GAG GAG GAG
`AGG AGG AGG GGT AGG AGG AGG AGG AGG
`GGT GGT GGT GGA GGT GGT GGT GGT GGT
`GGA GGA GGA GAG GGA GGA GGA GGG GGA
`GAT GGA GGA AAG GGA GAA GAA GAA GAG
`GGG GGG GGG GGG GGG GGG GGG GGG GGG
`GTT GTT GTT GTG CTT GTT GTT GTT GTT
`GAG GAG GAG GAG GAG GAG GAA GAG GAG
`TGG TGG TGG TGG TGG TGG TGG TGG TGG
`ATT ATT ATT GTG ATT ATT ATT ATT ATT
`GGA GGA GGA GGA GGA GGA GGT GGA GGA
`GAT AGG AGG TAG AGG AAT AAG AGG TAT
`ATT ATT ATT ATT ATT ATT ATT ATT ATT
`TAG GAT GAT AGT GAT AAT TAG GAT TAT
`GGT GGT GGT AGT GGT GGT GGT GGT GGT
`___ ___ ___
`___
`-.- ___ __- ___ ___
`___ ___ ___
`___
`___ ___ ___ ___ ___
`GGA AAT AAT
`GGG AAT GGG TGT TGT AGA
`GGT AGT AGT AGT AGT AAT GAT GAT GAT
`GGG GGT GGT AGT GGT GGT AGT AGT GGT
`TTT GGT GGT AGG GGT GGT GAA GAT AGT
`AGT AGT AGT GTG AGT AGT AGT AGT AGT
`AAG AAG AGG
`GAG AAG AAG GAG AAG AAT
`TAG TAG TAG TAT
`TAG TAG TAG TAG TAG
`AAT AAT AAT GGA AAT AAT AAT AAT AAT
`GAG GAG GAG GAG GAG GAG GAA GAA GAG
`AAT AAG GAG AGA AAG AAG AAG AAG AAG
`TTG TTG TTG GTG TTC TTG TTG TTG TTG
`AAG AAG AGG AAG AAG AAG AAG AAG AAG
`GGG AGG AGG GGG AGG AGG GAG GGG GGG
`AAG AAG AAG GGA AAG AAG AAG AAG AAG
`GGG GGG GGG TTG GGG GTG GGG GGG GGG
`AGA AGA AGA AGG
`AGA AGA AGA AGA AGA
`GTG GTG GTG ATG GTG GTG TTG TTG GTG
`AGT AGT AGT
`TGA AGT AGT AGT AGT AGT
`GGA GTA ATA AGA GTA GTA GTA GTA GGA
`GAG GAG GAG GAG GAG GAG GAG GAG GAG
`AGA AAA AAA AAT
`AGA AAA AAA AAA AGA
`TCC GGG GGG GGG
`TGG TGG TGG TGG TGG
`TGG TGG TGG AAG TGG TGG TGG TGG TGG
`AGG AGG AGG AAG
`AGG AGG AGG AGG AGG
`AGA AGA AGA AGG AGA AGA AGA AGA AGA
`GGG GGG GGG GTG GGG GGG GGG GGG GGG
`TAG TAG TAG TAG TTG TAG TAG TAG TAG TAG
`ATG ATG ATG ATG GTG ATG AGG ATG ATG ATG
`GAG GAG GAG GAG GAA GAG GAG GAG GAG GAG
`GTG GTG GTG GTG ATG GTG GTG GTG GTG GTG
`AAG AGG AGG AGG
`AGG
`AGG AGG AGG AGG AGG
`AGG AGG AGG AGG AGT
`
`_
`_
`_ _
`_
`_
`- _
`. _
`_ _
`_ _
`_ _
`_
`-
`___
`
`___
`
`___ _--
`___
`__,
`GAT GAG GAG GAG
`GTG TGG TAG TAG
`TGG TGG TGG TGG
`GGG GGG GGG GGG
`GGA GAA GAA GAA
`GGG GGG GGG GGG
`AGG AGG AGG AGG
`AGG AGT AGT AGT
`GTG GTG GTG GTG
`AGG AGA AGA AGA
`GTG GTG GTG GTG
`TGG TGG TGG TGG
`
`J
`
`Genzyme Ex. 1026, pg 727
`
`Genzyme Ex. 1026, pg 727
`
`

`
`249
`
`35
`BCL1
`
`31
`V
`S207
`
`S2
`V3
`
`39
`PMU3
`
`
`GAG
`TCA
`GTG
`AAG
`GAG
`TCC
`GAG
`GGA
`GGT
`CTC
`TTC
`AAG
`CCA
`ATG
`GAT
`ADC
`CTG
`ACA
`CTC
`ACC
`TGG
`ACA
`GTG
`TCT
`GGA
`TTC
`T00
`CTC
`AGT
`TCT
`TAT
`GGG
`GTG
`AAC
`——-
`
`
`
`———
`
`--— ——— --— --— ..... _.-
`
`_-
`
`-—_
`
`CODONS OF VARIABLE REGION HEAVV CHAINS (oonfd)
`37
`36
`34
`33
`Z3
`24
`25
`25
`27
`2B
`29
`'30
`SSGT VH441
`SP
`MC
`PCH
`PCH
`V1
`V11
`V13
`186-2
`145
`V1 0
`CHH»
`T15
`101
`108A 1085
`—
`—
`v v —
`7
`—
`-2 —
`—
`V
`_
`- —
`—— v
`D
`
`
`GAG
`GAG CAG GAG GAG GAG
`GAG GAA GAG GAG GAG GAG GAG GAG
`1
`
`GTG
`GT0 GTC GTG GTG GTG GTC GTC GTG GTG GTG GTG GTG GTT GTT
`2
`AAG
`CAG GAG AAG AAG AAG CAA CAA AAG AAG AAG GAG AAG CAG CAG
`3
`CTT
`CTT GTG CTG GTG CTG GTG GTG GTG GTG CTG CTG CTG GTG CTT
`4
`CTC
`CAG GAG GTG GTG ATG CAG CAG GTG GTG GTG AAG GTG CAG CAG
`5
`GAG
`CAG GAG GAA GAG GAG GAG GAG GAA GAA GAG CAG GAA GAG GAG
`6
`TCT
`TCA TCA TCT TCT TCT CCT CCT TCT TCT TCT TCA TCT TCT
`TCT
`7
`GGA
`GGA GGA GGA GGA GGA GGG GGG GGA GGA GGA GGA GGA GGG GGA
`E
`GGT
`CCT CCT GGA GGA GGA GCT GCT GGA GGA GGA CCT GGA CCT GCT
`9
`GGC
`GAG GAG GGC GGC GGG GAG GAG GGC GGG GGC GGG GGG GAG GAG
`10
`GTG
`CTG GTG TTG TTG TTG CTT CTT TTG TTG TTG GTG TTG GTG GTG
`11
`GTG
`GTG GTG GTA GTA GTA GTG GTG GTA GTA GTA GTG GTA GTG GTG
`12
`GAG
`AAA AAG CAG DAG CAG AAG AAG GAG CAG GAG CAB GAG AGG AGG
`13
`CCT
`CCT CCT CCT CCT CCT CCT CCT CCT CCT CCT GCC CCT CCT GCT
`14
`GGA
`GGG GGG GGG GGG GGG GGG GGG GGG GGG GGG TCA GGG GGG GGG
`15
`GGA
`GCC GGT GGT GGT GCT GGT GCT GGT GGT GGT CAG GGT GTC TCC
`16
`TCA TCA TCT TCT TCT TCA TCA TCT TCT TCT AGO ‘TCT TC-A TCA TCC
`17
`GTG GTG CTG GTG CTG GTG GTG GTG CTG GTG CTG CTG GTG GTG
`CTG
`18
`AAG AAG AGA AGA AGA AAG AAG AGA AGA AGA TCC AGA AAG AAG
`AAA
`19
`ATA ATA CTC CTC CTC CTG CTG CTC CTC CTC ATC CTC ATT ATG
`CTC
`20
`TCC ACC TCC TCC TCC TCC TCC TCC TCC TCC ACC TCC TCC TCC
`TCC
`21
`TGC TGC TGT TGT TGT TGC AGO TGT TGT TGT TGC TGT TGC TGG
`TGT
`22
`AAG AAG GCA GCA GAA AAG AAG GCA GCA GCA ACA GCA AAG AAG
`GCA
`23
`GCT GGT ACT ACT GGT GOT GOT ACT ACT TCT GTC ACT GGT GGT
`GCC
`24
`TCT TCT TCT TCT TCT TCT TCT TCT TCT TCT TCT TCT TCC TCT
`TCA
`25
`GGA GAT GGG GGG GGA GGG GGG GGG GGG GGG GGT GGG GGC GGA
`GGA
`26
`TAC TAC TTC TTC TTC TAC TAC TTC TTC TTC TTC TTC TAC TAT
`TTC
`27
`ACA TCA ACC ACC ACC ACC ACC ACC ACC ACC TCA ACC ACA ACA
`GAT
`28
`TTC TTC TTC TTG TTC TTC TTC TTC TTC TTG TTA TTC TTC TTC
`TTT
`29
`ACT ACT AGT ACT ACT ACC ACC AGT AGT ACT ACT AGT ACT
`ACA
`AGT
`30
`GAC GGC GAT GAT GAT AGC AGC GAT GAT GAT ACC GAT GAT AGC
`AGA
`31
`TAC TAO TTC TAC TAC TAG TAG TTC TTC TAG TAT TTC TAT
`TAC
`TAC
`32
`AAC ATC TAC TAC TAC TGG TGG TAC TAC TAC GGT TAC GCT GGT
`TGG
`33
`ATG ATG ATG ATG ATG ATG ATG ATG ATG ATG GTA ATG ATG ATA
`ATG
`34
`CAC AAC GAG AGC AGC CAC CAC GAG GAG AAC AAC GAG CAO AAC
`AGT
`35
`———
`———
`——— ——— ———
`———
`——— ——— ——— ——— ——— ———
`——A
`~——
`———
`35A
`--— --— --— --— -.-
`--— --— -Au
`--— -—-
`--— --— -——
`~———
`-—-
`353
`TGG TGG TGG TGG TGG TGG TGG TGG TGG TGG TGG TGG TGG TGG
`TGG
`36
`GTG GTG GTC GTC GTC GTG GTG GTC GTC GTG GTT GTC GTG GTG
`GTC
`37
`AAG AAG CGG CGG CGG AAG AAG CGG CGG GAG CGG CGC AAG AAA
`CGG
`38
`CAG CAG CAG CAG CAG GAG CAG CAG GAG CGG CAG CAG GAG GAG
`CAG
`39
`AGC AGC CDT CCT CCT AGG AGG CCT CCT CCT TGT CCT AGT
`AGG
`GGT
`40
`CAT CAT GCA CCA CCG CCT CCT CCA GCA CCA CCA CCA CAT CCT
`CCA
`41
`
`GGA GGA GGG GGA AGG GGA GGA GGG GGG GGG GGA GGG GCA GGAAAG AAG AAG AAG AAG CGA CGA AAG AAG AAG AAG AAG AAG CAG
`
`GGGAAA
`
`4243
`AGC AGC AGA GCA GCA GGC GGG AGA AGA GCA GGT AGA AGT
`GGC
`GGG
`44
`CTT CTT CTG CTT CCT CTT CTT GTG GTG CTT GTG CTG CTA CTG CTA
`45
`GAG GAA GAG GAG GAG GAG GAG GAG GAG GAG GAG GAG GAG GAA
`GAA
`-16
`TGG TGG TGG TGG TGG TGG TGG TGG TGG TAG TGG TGG TGG TGG
`TGG
`47
`ATT ATT ATT TTG TTG ATT ATT ATT ATT TTG CTG ATT ATT ATT
`ATT
`48
`GGA GGA GCT GGT GCT GGA GGA GGT GGT GCT GGA GCT GGA GGA
`GGA
`49
`TAT GAA GCA TTT TTG AGG AGG GCA GCA TTG GTG GGA GTT
`TAT
`GAA
`50
`ATT ATT AGT ATT ATT ATT ATT AGT AGT ATT ATA AGT ATT ATT
`ATT
`51
`TAT AAT AGA AGA AGA GAT GAT AGA AGA AGA TGG AGA AGT AAT
`AAT
`52
`52A C-CT CCT AAC AAC AAC CCT CCT AAC AAG AAC AGT AAC ACT
`CCT
`CCA
`52B _.-
`___ AAA AAA AAA ___
`___ AAA AAA AAA GGT AAA .--
`‘V-
`-—7
`52C
`77— _—7 GCT GGT GCT -—7 —77 GCT GCT GCT GGA GGT 777
`777
`772
`53
`TAC TAC AAT AAT AAT AAT AAT AAT AAT AAT AGO AAT TAC GGA
`GAT
`54
`AAT AAT GAT GGT GGC AGT AGT GAT GAT GGT ACG GAT AAT AAT
`AGC
`55
`GGT GGT TAT TAC TAT GGT GGT TAT TAT TAC GAG TAT GGT GGT
`AGT
`55
`GGT GGT ACA ACA ACA GGT GGT ACA ACA ATA TAT ACA AAT
`TAT
`ACG
`57
`ACT ACT ACA ACA ACA ACT ACT ACA ACA ACA AAT ACA ACG AAT
`ATA
`58
`GGG AGC GAG GAG GAG AAG AAG GAG GAG GAG GCA GAG AGO AAG
`AAC
`59
`TAC TAC TAC TAC TAT TAC TAC TAG TAC TAC GGT TAC TAC TAC
`TAT
`S0
`AAC AAC AGT AGT AGT AAT AAT AGT AGT AGT TTC AGT AAC AAT
`ACG
`E1
`GAG GAG GCA GCA GCA GAG GAG GCA GCA GGA ATA GCA GAG GAG
`CCA
`E2
`AAG AAG TCT TCT TCT AAG AAG TCT TCT TCT TCC TCT AAG AAG
`TCT
`63
`TTC TTC GTG GTG GT!T TTC TTC GTG GTG ATG AGA GTG TTT TTC
`CTA
`64
`AAG AAG AAG AAG AAG AAG AAG AAG AAG AAG CTG AAG AAG AAG
`AAG
`65
`AGC GGG GGT GGT GGT AGC AGO GGT GGT GGT AAC GGT GGG GGG
`GAT
`E6
`AAG AAG CGG GGG CGG AAG AAG CGG CGG CGG ATC CGG AAG AAG
`AAA
`67
`GCC GCC TTC TTC TTC GCC GCC TTC TTC TTC ACC TTC GCC ACC
`TTC
`68
`ACA ACA ATC ACC ACC ACA ACA ATC ATC ACC AAG ATC ACA ACA
`ATC
`69
`TTG TTG GTC ATC ATC GTG CTG GTC GTC ATC GAC GTC ATG CTG
`ATC
`70
`ACT ACT TCC TCC TCC ACT ACT TCC TCC TCC AAT TCC ACT ACT
`TCC
`71
`GTA GTA AGA AGA AGA GTA GTA AGA AGA AGA TCC AGA GTA GTA
`AGA
`72
`GAC GAG GAG GAT GAT GAC GAG GAC GAG GAT AAG GAG GAG GAC
`GAC
`73
`AAT ACA ACT AAT AAT AAA AAA ACT ACT AAT AGC ACT AAA AAA
`AAC
`74
`TCC TCC TCC TCC TCT GCC CGG TCC TCC TCC CAA TCC TCC TCC
`GCC
`75
`TCC TCC CAA CAA GAA TCC TCC CAA CAA CAA GTT CAA TCC TCC
`AAA
`76
`AGC AGC AGO AGG AAC AGO AGG AGO AGO AGO TTC AGC AGC AGC
`AAT
`77
`ACA ACA ATC ATC ATC ACA ACA ATC ATC ATC TTT ATC ACA ACA
`ACG
`75
`GCC GGG CTC CTC CTC GCC GCC CTC CTC CTC AAA GTG GTC GCC
`GTG
`79
`TAC TAC TAC TAT TAT TAG TAC TAC TAC TAT ATG TAC CAT
`TAC
`TAC
`30
`ATG ATG CTT CTT CTT ATG ATG CTT CTT CTT AAC CTT ATG ATG
`GTG
`81
`GAG GAG GAG CAA CAA GAG GAG CAG GAG CAA AGT CAG GAA CAG
`CAA
`B2
`CTC CTC ATG ATG ATG CTC CTC ATG ATG ATG GTG ATG CTT CTC
`ATG
`52A AGC CAC AAT AAC AAC AGC AGC AAT AAT AAC CAA AAT GCC AGA
`AGO
`B2B AGC AGC GCC ACC ACC AGC AGC GCC GCC ACA TCT GCC AGA AGC
`AAA
`82C CTG GTG GTG CTC-i GTG GTG CTG CTG CTG CTG AAT GTG TTG CTG
`GTG
`33
`AGA ACA AGA AGA AGA ACA ACA AGA AGA AGO GAC AGA ACA ACA
`AGA
`84
`TCT TCT GCT GGT GOT TCT TCT GCT GCT
`#
`ACA GCT TCT
`TGT
`TC-T
`85
`GAS GAG GAG GAG GAG GAG GAG GAG GAG GAG GCC GAG GAG GAG
`GAG
`66
`GAC GAG GAG GAG GCC GAG GAG GAG GAG GAO ATA GAG GAT GAC
`GAG
`87
`TOT TCT ACT AGT AGT TCT TCT ACT ACT AGT TAT ACT TCT
`TCT
`ACA
`36
`GCA TTG GCC GCC GCC GGG GGG GCC GCC GCC TAC GCC GCC GCA
`GCC
`89
`GTC GTC ATT ACT ACT GTC GTC ATT ATT ACT TGT ATT AAT GTC
`CTT
`90
`TAT TAT TAT