CURRICULUM VITAE
`Robert J. Linhardt
`
`Year Conferred
`1975
`
`1977, 1979
`1979-1982
`
`
`
`
`
`
`
`
`
`
`
`1992
`1989
`1981
`1979
`
`Field of Study
`Chemistry
`Organic Chemistry
`Biochemical Engineering
`
`EDUCATION
`Degree
`
`
`Institution and Location
`B.S.
`
`
`
`Marquette University
`M.A., Ph.D.
`
`The Johns Hopkins University
`Postdoctoral
`Massachusetts Institute of Technology
`RESEARCH AND PROFESSIONAL EXPERIENCE
`9/03-
` Ann and John H. Broadbent Senior Constellation Chair in Biocatalysis and Metabolic Engineering, Professor of
`Chemistry, Biology, Chemical and Biological Engineering, Biomedical Engineering, Rensselaer Polytechnic Institute
`7/06-8/08 Acting Director, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
`1/15-
` Adjunct Professor, Orthopaedics, Mount Sinai School of Medicine, NY, NY
`7/05-
` Adjunct Professor of Pharmaceutical Sciences, Albany College of Pharmacy, Albany, NY
`7/99-8/03 F. Wendell Miller Distinguished Professor of Chemistry
`5/96-8/03 F. Wendell Miller Distinguished Professor of Medicinal and Natural Products Chemistry and Professor of Chemical and
`Biochemical Engineering
`5/90-8/03 Professor of Medicinal and Natural Products Chemistry
`1/92-9/92 Visiting Professor, University of California, San Diego, Cancer Center and the La Jolla Cancer Research Foundation
`7/86-4/90 Associate Professor of Medicinal and Natural Products Chemistry
`7/82-6/86 Assistant Professor of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa (UI)
`1/81-
`
`Industrial Consultant in the area of biocatalysis, biopolymers, carbohydrate chemistry and drug delivery
`6/79-6/82 Research Associate, Biochemical Engineering, Department of Applied Biological Sciences and Harvard-MIT Joint
`Program in Biomedical Research at the Whitaker College of Health Sciences and Technology, MIT
`9/75-5/79 Research/Teaching Assistant, Department of Chemistry, The Johns Hopkins University
`PROFESSIONAL SOCIETIES
`American Chemical Society, Divisions of Medicinal Chemistry, Biotechnology and Carbohydrate Chemistry; American
`Association for the Advancement of Science – AAAS Fellow; Society for Glycobiology; American Association of
`Colleges of Pharmacy; American Association of Pharmaceutical Scientists; American Society of Biochemistry and
`Molecular Biology; American Society for Matrix Biology
`NATIONAL APPOINTMENTS
`Editorial Boards: Journal of Biological Chemistry 1995-2000, 2005-2011; Journal of Carbohydrate Chemistry 1995-;
`
`Analytical Biochemistry 1991-1997, 2001-; Carbohydrate Research 1990-2002; Applied Biochemistry and Biotechnology
`1985-; Current Organic Synthesis (regional editor) 2002-; Mini Reviews in Organic Chemistry (regional editor) 2002-,
`Glycobiology 2006-, Nanotechnology, Science and Applications 2007-, Biochemistry Research International, 2007-, Open
`Glycoscience (editor-in-chief), 2007-2010, Organic Synthesis, 2008-, Cell Health and Cytoskeleton, 2008-, International
`Journal of Carbohydrate Chemistry 2008-, Molecules 2009-, Polymers 2009-; American Journal of Analytical Chemistry
`2010-; Journal of Advanced Research 2011-; IET Nanobiotechnology 2013-; Carbohydrate Polymers 2014-. 2012 Chair–
`Proteoglycans Gordon Conference; National Institutes of Health, Pharmacology Special Study Section, 1988, Bioorganic
`and Natural Products Study Section ad hoc member 2003, Thrombosis and Hemostasis Study Section ad hoc member
`2004, Synthetic and Biological Chemistry A Study Section ad hoc member 2006, and Synthetic and Biological
`Chemistry B, Biological Chemistry and Macromolecular Biophysics ad hoc member 2009. FDA Adverse Reactions
`Working Group, 2008, USP Advisory Committee on Heparin, 2008-2009, Presidents Council on Competitiveness
`(National Initiative on Innovation, Skills subcommittee member) 2004. National Academy of Sciences Interacademy
`Exchange Scientist to Russia and the Ukraine, 1987.
`ACADEMIC HONORS
`2010
` Elected to the National Academy of Inventors (NAI)
`2010
` Melville L. Wolfrom Award from the American Chemical Society, Division of Carbohydrate Chemistry for
`
`
`outstanding service to the Carbohydrate Division and to the field of carbohydrate chemistry
`2010
` Elected to the rank of American Association for the Advancement of Science (AAAS) Fellow
`2010
` USP Award for an Innovative Response to a Public Health Challenge
`2009
`
`Scientific American 10 - Recognized for contributions to American science and technology
`2006
` Gisvold Award in Medicinal and Natural Products Chemistry from the University of Minnesota
`2003
` RPI Ann and John H. Broadbent Senior Constellation Chair in Biocatalysis and Metabolic Engineering awarded
`2003
` American Chemical Society Claude S. Hudson Award in Carbohydrate Chemistry
`1999
`Volwiler Research Achievement Award sponsored by Abbott, awarded by the AACP
`1996
` University of Iowa F. Wendell Miller Distinguished Professorship Awarded
`1994
` Horace S. Isbell Award from the American Chemical Society, Division of Carbohydrate Chemistry - Given for excellence
`of contributions to carbohydrate chemistry and continuing promise of quality contributions.
`Iowa Regents Award for Faculty Excellence - For sustained excellence across the spectrum of faculty endeavors.
`
` University of Iowa Excellence in Teaching Award
` MIT/Johnson & Johnson Postdoctoral Fellow - A competitive award given for exceptional biomedical research.
` The Johns Hopkins University/Ernst M. Marks Award - Presented for excellence in teaching and research in organic
`chemistry.
`
`MYLAN ET AL. - EXHIBIT 1003
`
`0001
`
`

`
`PUBLICATIONS
`1.
`"Mechanism of Decomposition for Diacyl Peroxides and Related Carboxy Inversion Compounds in Aqueous Acetone," R.J.
`Linhardt, B.L. Murr, Tetrahedron Letters, 12, 1007-1010, 1979.
`
`"The Mechanism of Diacyl Peroxide Decomposition," R.J. Linhardt, Ph.D. Dissertation, The Johns Hopkins University,
`1979.
`
`"Heparinase Production by Flavobacterium heparinum," P.M. Galliher, C.L. Cooney, R. Langer and R.J. Linhardt, Applied
`and Environmental Microbiology, 41, 360-365, 1981. PMID: 7235692, PMCID: PMC243699
`
`"Blood Deheparinization Using Immobilized Microbial Heparinase," R.J. Linhardt, R. Langer, C.L. Cooney, P.M. Galliher,
`M.M. Flanagan, S.M. Hoffberg, Proceedings of the Second World Congress of Chemical Engineering, Montreal, Canada,
`Vol. I, pp. 182-186, 1981.
`
`"Mechanism for Diacyl Peroxide Decomposition," R.J. Linhardt, B.L. Murr, E. Montgomery, J. Osby and J. Sherbine, The
`Journal of Organic Chemistry, 47, 2242-2251, 1982.
`
`"A System for Heparin Removal," R. Langer, R.J. Linhardt, P.M. Galliher, M.M. Flanagan, C.L. Cooney and, Klein, M.D.
`Biomaterials: Interfacial Phenomena and Applications, Advances in Chemistry Series, Chapter 31, pp. 493-509, S.L. Cooper
`and N.A. Pepas (Eds.), Washington, D.C. 1982.
`
`"Immobilized Heparinase: Production, Purification, and Application In Extracorporeal Therapy," R. Langer, R.J. Linhardt,
`C.C. Cooney, D. Tapper, M.D. Klein, Enzyme Engineering Vol. 6, pp. 433-441, I. Chibata, S. Fukui and L.B. Wingard, Jr.
`(Eds.), Plenum Press, New York, 1982.
`
`"Mode of Action of Heparin Lyase On Heparin," R.J. Linhardt, G.L. Fitzgerald, C.L. Cooney, R. Langer, Biochimica et
`Biophysica Acta, 702, 197-203, 1982.
`
`"An Enzymatic System for Removing Heparin in Extracorporeal Therapy," R. Langer, R.J. Linhardt, S. Hoffberg, A.K.
`Larsen, C.L. Cooney, D. Tapper, M. Klein, Science, 217, 261-263, 1982.
`
`"Regulation of Heparinase Synthesis in Flavobacterium heparinum," P.M. Galliher, R.J. Linhardt, L.J. Conway, R. Langer,
`C.L. Cooney, European Journal of Applied Microbiology and Biotechnology, 15, 252-257, 1982.
`
`"Differential Anticoagulant Activity of Heparin Fragments Prepared Using Microbial Heparinase," R.J. Linhardt, A. Grant,
`C.L. Cooney, R. Langer, The Journal of Biological Chemistry, 257, 7310-7313, 1982.
`
`"A Colorimetric Assay for Chemical Heparin in Plasma," M. D. Klein, R.A. Drongowski, R.J. Linhardt, R.S. Langer,
`Analytical Biochemistry, 124, 59-64, 1982.
`
`"In Vivo Activity of Microbial Heparinase," R. Langer, R.J. Linhardt, A.K. Larsen, C.L. Cooney, D. Tapper, M. Klein,
`Transactions of the American Society for Artificial Internal Organs, 28, 387-390, 1982.
`
`"Bioerodible Polyanhydrides For Controlled Drug Delivery," H. B. Rosen, J. Chang, G. E. Wnek, R.J. Linhardt, R. Langer,
`Biomaterials, 4, 131-133, 1983.
`
`"Heparinase: In Vivo Activity and Immunogenicity in Rabbits," M.D. Klein, R.A. Drongowski, R.J. Linhardt, C.L. Cooney,
`R.S. Langer, Journal of Laboratory and Clinical Medicine, 102, 828-837, 1983.
`
`"Angiogenesis Inhibition and Tumor Regression Caused by Heparin or a Heparin Fragment in the Presence of Cortisone," J.
`Folkman, R. Langer, R.J. Linhardt, C. Haudenschild, S. Taylor, Science, 221, 719-725, 1983.
`
`"Bioerodable Polyanhydrides for Controlled Drug Delivery," R.J. Linhardt, H.B. Rosen, R. Langer, Polymer Preprints, 24,
`47-48, 1983.
`
`"An Immobilized Microbial Heparinase for Blood Deheparinization," R.J. Linhardt, C.L. Cooney, D. Tapper, C.A. Zannetos,
`A.K. Larsen, R. Langer, Applied Biochemistry and Biotechnology, 9, 41-55, 1984.
`
`"Metachromatic Activity of Heparin and Heparin Fragments," A.C. Grant, R.J. Linhardt, G.L. Fitzgerald, J.J. Park, R.
`Langer, Analytical Biochemistry, 137, 25-32, 1984.
`
`"Effect of Extracorporeal Enzymatic Deheparinization on Formed Blood Components," A.K. Larsen, R.J. Linhardt, D.
`Tapper, M. Klein, R. Langer, Artificial Organs, 8, 198-203, 1984.
`
`
`2.
`
`
`3.
`
`
`4.
`
`
`5.
`
`
`6.
`
`
`7.
`
`
`8.
`
`
`9.
`
`
`10.
`
`
`11.
`
`
`12.
`
`
`13.
`
`
`14.
`
`
`15.
`
`
`16.
`
`
`17.
`
`
`18.
`
`
`19.
`
`
`20.
`
`
`
`0002
`
`

`
`"Polymer Based Drug Delivery: Magnetic Modulated and Bioerodible Systems," E.R. Edelmen, R.J. Linhardt, H. Bobeck, J.
`Kost, H.B. Rosen, R. Langer, Polymers as Biomaterials, pp. 279-292, S.W. Shalaby, A.S. Hoffman, B.D. Ratner and T.A.
`Horbett (Eds.) Plenum Press, 1984.
`
`"Purification and Characterization of Heparinase from Flavobacterium heparinum," V.C. Yang, R.J. Linhardt, H. Bernstein,
`C.L. Cooney, R. Langer, The Journal of Biological Chemistry, 260, 1849-1857, 1985.
`
`"Small Heparin Fragments Regulate the Amplification Pathway of Complement," M.D. Sharath, Z.M. Merchant, Y.S. Kim,
`K.G. Rice, R.J. Linhardt, J.M. Weiler, Immunopharmacology, 9, 73-80, 1985.
`
`"Structure of Heparin-Derived Tetrasaccharides," Z.M. Merchant, Y.S. Kim, K.G. Rice, R.J. Linhardt, Biochemical Journal,
`229, 369-377, 1985. PMID: 4038274, PMCID: PMC1145069
`
`"Immuno-Affinity Purification of Heparinase," R.J. Linhardt, Z.M. Merchant, D.W. Persinger, The International Journal of
`Biochemistry, 17, 1179-1183, 1985.
`
`"High-Performance Liquid Chromatographic Separation of Heparin-Derived Oligosaccharides," K.G. Rice, Y.S. Kim, A.C.
`Grant, Z.M. Merchant, R.J. Linhardt, Analytical Biochemistry, 150, 325-331, 1985.
`
`"Evidence of Random Structural Features in the Heparin Polymer," R.J. Linhardt, Z.M. Merchant, K.G. Rice, Y.S. Kim, G.L.
`Fitzgerald, A.C. Grant, R. Langer, Biochemistry, 24, 7805-7810, 1985.
`
`"Polysaccharide Lyases," R.J. Linhardt, P.M. Galliher, C.L. Cooney, Applied Biochemistry and Biotechnology, 12, 135-176,
`1986.
`
`"Effect of Heparin, Heparin Fragments, and Corticosteriods on Cerebral Endothelial Cell Growth In Vitro and In Vivo," D.W.
`Beck, J.J. Olson, R.J. Linhardt, Journal of Neuropathology and Experimental Neurology, 45, 503-512, 1986.
`
`"Variation in Composition and Yield of Exopolysaccharides Produced by Klebsiella sp. Strain K32 and Acinetobacter
`calcoaceticus BD4," B.A. Bryan, R.J. Linhardt, L. Daniels, Applied and Environmental Microbiology, 51, 1304-1308, 1986.
`PMID: 3729401, PMCID: PMC239062
`
`"Effect of Very Low Molecular Weight Heparin-Derived Oligosaccharides on Lipoprotein Lipase Release in Rabbits," Z.M.
`Merchant, E.E. Erbe, W.P. Eddy, D. Patel, R.J. Linhardt, Atherosclerosis, 62, 151-158, 1986.
`
`"Structure and Activity of a Unique Heparin-Derived Hexasaccharide," R.J. Linhardt, K.G. Rice, Z.M. Merchant, Y.S. Kim,
`D.L. Lohse, The Journal of Biological Chemistry, 261, 14448-14454, 1986.
`
`"New Approaches for Anticoagulation in Extracorporeal Therapy," R.J. Linhardt, R. Langer, Journal of Biomaterials,
`Artificial Cells and Artificial Organs, 15, 91-100, 1987.
`
`"Fractionation of Heparin-Derived Oligosaccharides by Gradient Polyacrylamide-Gel Electrophoresis," K.G. Rice, M.K.
`Rottink, R.J. Linhardt, Biochemical Journal, 244, 515-522, 1987. PMID: 3446173, PMCID: PMC1148026
`
`"Use of Computer Simulation on the Massively Parallel Processor to Study the Structural Features of Heparin," D.M. Cohen,
`R.J. Linhardt, Proceedings of the Third International Conference on Super-computing: Supercomputing Projects,
`Applications and Artificial Intelligence, Volume 1, pp 210-213, L.P. Kartashev and S.I. Kartashev, (Eds.), International
`Supercomputing Institute, St. Petersburg, FL, 1988.
`
`"Homogeneous, Structurally Defined Heparin-Oligosaccharides With Low Anticoagulant Activity Inhibit the Generation of
`the Amplification Pathway C3 Convertase In Vitro," R.J. Linhardt, K.G. Rice, Y.S. Kim, J. D. Engelken, J. M. Weiler, The
`Journal of Biological Chemistry, 263, 13090-13096, 1988.
`
`"Microheterogeneity of Plasma Glycoproteins Heparin Cofactor II and Antithrombin III and Their Carbohydrate Analysis,"
`Y.S. Kim, K.B. Lee and R.J. Linhardt, Thrombosis Research, 51, 97-104, 1988.
`
`"Mapping and Quantification of the Major Oligosaccharide Components of Heparin," R.J. Linhardt, K.G. Rice, Y.S. Kim,
`D.L. Lohse, H.M. Wang, D. Loganathan," Biochemical Journal, 254, 781-787, 1988. PMID: 3196292, PMCID:
`PMC1135151
`
`"Microbially Produced Rhamnolipid as a Source of Rhamnose," R.J. Linhardt, R. Bakhit, L. Daniels, F. Mayerl, and W.
`Pickenhagen, Biotechnology and Bioengineering, 33, 365-368, 1989.
`
`21.
`
`
`22.
`
`
`23.
`
`
`24.
`
`
`25.
`
`
`26.
`
`
`27.
`
`
`28.
`
`
`29.
`
`
`30.
`
`
`31.
`
`
`32.
`
`
`33.
`
`
`34.
`
`
`35.
`
`
`36.
`
`
`37.
`
`
`38.
`
`
`39.
`
`
`
`0003
`
`

`
`"Study of Structurally Defined Oligosaccharide Substrates of Heparin and Heparan Monosulfate Lyases," K.G. Rice and R.J.
`Linhardt, Carbohydrate Research, 190, 219-233, 1989.
`
`"A Dose Escalation Study of Org 10172 (Low Molecular Weight Heparinoid) in Stroke," J. Biller, E.W. Massey, J.R. Marler,
`H.P. Adams, J.N. Davis, A. Bruno, R.A. Henriksen, R.J. Linhardt, L.B. Goldstein, M. Alberts, C.T. Kisker, G.J. Toffol, C.S.
`Greenberg, K.J. Banwart, C. Bertels, D.W. Beck, M. Walker, H.N. Maganani, Neurology, 39, 262-265, 1989.
`
`"Resistance of Heparinase-Derived Heparin Fragments to Biotransformation," A.K. Larsen, K.G. Rice, RJ. Linhardt, G.
`Wogan, R. Langer, The Journal of Biological Chemistry, 264, 1570-1577, 1989.
`
`"Biodegradable Polymers For Controlled Release of Drugs," R.J. Linhardt, in Controlled Release of Drugs: Polymers and
`Aggregate Systems, Verlag Chemie Critical Review Series, M. Rosoff (Ed.), Chapter 2, pp 53-95, 1989.
`
`"Nonrandom Structural Features in the Heparin Polymer," R.J. Linhardt, D.M. Cohen, K.G. Rice, Biochemistry, 28, 2888-
`2894, 1989.
`
`"Structural Features of Heparin and Their Effect on Heparin Cofactor II Mediated Inhibition of Thrombin," Y.S. Kim and
`R.J. Linhardt, Thrombosis Research, 53, 55-71, 1989.
`
`"Quantitative Analysis of the Monomer Products Formed on the Hydrolysis of Poly(Esters) and Poly(Anhydrides)," R.J.
`Linhardt, D.R. Flanagan, E. Schmitt, H.T. Wang, Polymer Preprints, 30, 464-465, 1989.
`
`"Comparison of the Activity of Polyanions and Polycations on the Classical and Alternative Pathways of Complement," J.M.
`Weiler, R.J. Linhardt, Immunopharmacology, 17, 65-72, 1989.
`
`"Sequence Analysis of Highly Sulfated Heparin-Derived Oligosaccharides Using Fast Atom Bombardment Mass
`Spectrometry," L.M. Mallis, H.M. Wang, D. Loganathan, R.J. Linhardt, Analytical Chemistry, 61, 1453-1458, 1989.
`
`"Analysis of Glycosaminoglycan-Derived Oligosaccharides Using Reversed Phase Ion-Pairing and Ion-Exchange
`Chromatography With Suppressed Conductivity Detection," R.J. Linhardt, K.N. Gu, D. Loganathan, S.R. Carter, Analytical
`Biochemistry, 181, 288-296, 1989.
`
`"Structure of Amiprilose-Hydrochloride, A Novel Anti-Inflammatory Agent," R.J. Linhardt, N.C. Baenziger, B. Ronsen,
`Journal of Pharmaceutical Science, 79, 158-162, 1990.
`
`"Heparin, Heparinoids and Heparin Oligosaccharides: Structure and Biological Activities," R.J. Linhardt and D. Loganathan,
`in Biomimetic Polymers, G. Gebelein (ed.), Plenum Press, New York, pp. 135-173, 1990.
`
`"Carbohydrate Analysis of Glycoproteins," K.B. Lee, D. Loganathan, Z.M. Merchant, R.J. Linhardt, Applied Biochemistry
`and Biotechnology, 23, 53-80, 1990.
`
`"Isolation and Recovery of Acidic Oligosaccharides from Polyacrylamide Gels by Semi-Dry Electrotransfer," A. Al-Hakim,
`R.J. Linhardt, Electrophoresis, 11, 23-28, 1990.
`
`"Structural Variation in the Antithrombin III Binding Site Region and Its Occurrence in Heparin from Different Sources," D.
`Loganathan, H.M. Wang, L.M. Mallis, R.J. Linhardt, Biochemistry, 29, 4362-4368, 1990.
`
`"Examination of the Substrate Specificity of Heparin and Heparan Sulfate Lyases," R.J. Linhardt, J.E. Turnbull, H.M. Wang,
`D. Loganathan, J.T. Gallagher, Biochemistry, 29, 2611-2617, 1990.
`
`"Modification and Potential Uses of Heparin and Other Acidic Polysaccharides," R.J. Linhardt, Proceedings of the ACS
`Division of Polymeric Materials: Science and Engineering, 62, 488-491, 1990.
`
`"Biodegradable Poly(Esters) and the Delivery of Bioactive Agents," R.J. Linhardt, D.R. Flanagan, E. Schmitt, H.T. Wang,
`Polymer Preprints, 31, 249-250, 1990.
`
`"Oligosaccharide Mapping of Low Molecular Weight Heparins: Structure and Activity Differences," R.J. Linhardt, D.
`Loganathan, A. Al-Hakim, H.M. Wang, J.M. Walenga, D. Hoppensteadt, J. Fareed, Journal of Medicinal Chemistry, 33,
`1639-1645, 1990.
`
`"Randomness in the Heparin Polymer: Computer Simulations of Alternative Action Patterns of Heparin Lyase," D.M.
`Cohen, R.J. Linhardt, Biopolymers, 30, 733-741, 1990.
`
`40.
`
`
`41.
`
`
`42.
`
`
`43.
`
`
`44.
`
`
`45.
`
`
`46.
`
`
`47.
`
`
`48.
`
`
`49.
`
`
`50.
`
`
`51.
`
`
`52.
`
`
`53.
`
`
`54.
`
`
`55.
`
`
`56.
`
`
`57.
`
`
`58.
`
`
`59.
`
`
`
`0004
`
`

`
`"Degradation of Poly(Ester) Microspheres," H.T. Wang, H. Palmer, R.J. Linhardt, D.R. Flanagan, E. Schmitt, Biomaterials,
`11, 679-685, 1990.
`
`"Low-Molecular-Weight Heparins and Heparinoids and their Use in Acute or Progressing Ischemic Stroke," D.L. Gordon, R.
`Linhardt, H.P. Adams, Clinical Neuropharmacology, 13, 522-543, 1990.
`
`"Structure and Metabolism of Glycosaminoglycans," R.J. Linhardt, D. Loganathan A. Al-Hakim, S. A. Ampofo, in New
`Trends in Haemostasis: Coagulation Proteins, Endothelium, and Tissue Factors, Springer Verlag Publishers, Berlin, J.
`Harenberg, D.L. Heene, G. Stehle, G. Schletter, (Eds.), pp. 12-26, 1990.
`
`"Biodegradable Poly(Esters) for the Controlled Delivery of Vaccines," R.J. Linhardt, H.T. Wang, D.R. Flanagan, E. Schmitt,
`H. Palmer, Proceedings of the ACS Division of Polymeric Materials: Science and Engineering, 63, 39-42, 1990.
`
`"Immobilization of C3b with Retention of Functional Activity," R.E. Edens, R.J. Linhardt, J.M. Weiler, Journal of
`Immunological Methods, 133, 67-70, 1990.
`
`"Electrophoresis and Detection of Nanogram Quantities of Exogenous and Endogenous Glycosaminoglycans in Biological
`Fluids," A. Al-Hakim, R.J. Linhardt, Applied and Theoretical Electrophoresis, 1, 305-312, 1991.
`
`"Biocatalysts for the Synthesis and Modification of Biopolymers," R.J. Linhardt and A. Al-Hakim in Biocatalysts for
`Industry, Topics in Applied Chemistry, Plenum Publishing Company, New York, J.S. Dordick (Ed.), Chapter 5, pp. 83-112,
`1991.
`
`"Molecular Profile and Mapping of Dermatan Sulfates from Different Origins," R.J. Linhardt, A. Al-Hakim, S.Y. Liu, Y.S.
`Kim, J. Fareed, Seminars in Thrombosis and Hemostasis, 17, 15-22, 1991.
`
`"A New Method for Sequencing Linear Oligosaccharides on Gels Using Charged, Fluorescent Conjugates," K.B. Lee, A. Al-
`Hakim, D. Loganathan, R.J. Linhardt, Carbohydrate Research, 214, 155-168, 1991.
`
`"Acidic Polysaccharides, Their Modification and Potential Uses," R.J. Linhardt, A. Al-Hakim, J. Liu, in Biotechnology and
`Polymers, Plenum Press Publishing Company, New York, C.G. Gebelein (Ed.) 1991, pp. 115-165.
`
`"Influence of Formulation Methods on the In Vitro Controlled Release of Protein from Poly(Ester) Microspheres," H.T.
`Wang, E. Schmitt, D.R. Flanagan, R.J. Linhardt, Journal of Controlled Release, 17, 23-32, 1991.
`
`"Rhamnose from the Products of Microbial Fermentation," R.J. Linhardt, SIM Industrial Microbiology News, 41, 113-118,
`1991.
`
`"Heparin: An Important Drug Enters its Seventh Decade," R.J. Linhardt, Chemistry & Industry, 2, 45-50, 1991.
`
`"Antithrombin III Regulates Complement Activity In Vitro," J.M. Weiler, R.J. Linhardt, Journal of Immunology, 146, 3889-
`3894, 1991.
`
`"Determination of the pKa of Glucuronic Acid and the Carboxy Groups of Heparin by 13C-Nuclear-Magnetic Resonance
`Spectroscopy," H.M. Wang, D. Loganathan, R.J. Linhardt, Biochemical Journal, 278, 689-695, 1991. PMID: 1898357,
`PMCID: PMC1151401
`
`"Structural Features of Dermatan Sulfates and their Relationship to Anticoagulant and Antithrombotic Activities," R.J.
`Linhardt, A. Al-Hakim, J. Liu, D. Hoppensteadt, G. Mascellani, P. Bianchini, J. Fareed Biochemical Pharmacology, 42,
`1609-1619, 1991.
`
`"Capillary Electrophoresis for the Analysis of Chondroitin Sulfate- and Dermatan Sulfate-Derived Disaccharides," A. Al-
`Hakim, R.J. Linhardt, Analytical Biochemistry, 195, 68-73, 1991.
`
`"Capillary Zone Electrophoresis for the Quantitation of Oligosaccharides Formed Through the Action of Chitinase", K.B.
`Lee, Y.S. Kim, R.J. Linhardt, Electrophoresis, 12, 636-640, 1991.
`
`"Disaccharide Compositional Analysis of Heparin and Heparan Sulfate Using Capillary Zone Electrophoresis," S.A. Ampofo,
`H.M. Wang, R.J. Linhardt, Analytical Biochemistry, 199, 249-255, 1991.
`
`"Detection of Chitinase Activity Using a Fluorescence-Labeled Substrate on Polyacrylamide Gel," Y.S. Kim, K.B. Lee, R.J.
`Linhardt, Korean Biochemical Journal, 24, 466-471, 1991.
`
`60.
`
`
`61.
`
`
`62.
`
`
`63.
`
`
`64.
`
`
`65.
`
`
`66.
`
`
`67.
`
`
`68
`
`
`69.
`
`
`70.
`
`
`71.
`
`
`72.
`
`73.
`
`
`74.
`
`
`75.
`
`
`76.
`
`
`77.
`
`
`78.
`
`
`79.
`
`
`
`0005
`
`

`
`80.
`
`
`81.
`
`
`82.
`
`
`83.
`
`
`84.
`
`
`85.
`
`
`86.
`
`
`87.
`
`
`88.
`
`
`89.
`
`
`90.
`
`
`91.
`
`
`92.
`
`
`93.
`
`
`94.
`
`
`95.
`
`
`96.
`
`
`97.
`
`
`98.
`
`
`
`"Controlled Release of Protein and Vaccines from Poly(Ester) Microspheres In Vitro," H.T. Wang, H. Palmer, R.J. Linhardt,
`D.R. Flanagan, E. Schmitt, in Cosmetic and Pharmaceutical Applications of Polymers, Plenum Press Publishing Company,
`New York, C.G. Gebelein, T.C. Cheng, V.C. Yang (Eds.), pp 239-253, 1991.
`
`"Controlled Release of Protein and Vaccines from Poly(Ester) Microspheres In Vitro," H.T. Wang, H. Palmer, R.J. Linhardt,
`D.R. Flanagan, E. Schmitt, Proceedings of the ACS Symposium on Polymers Cosmetics and Pharmaceutical Applications,
`239-253, 1991.
`
`"Chemical and Enzymatic Methods for the Depolymerization and Modification of Heparin," R.J. Linhardt, in Carbohydrates-
`Synthetic Methods and Applications in Medicinal Chemistry, Kodansha/VCH Publishers, Tokyo/Weinheim, H. Ogura, A.
`Hasegawa, T. Suami (Eds.), Chap. 20, pp 387-403, 1992.
`
`"Analysis of Glycosaminoglycan-Derived Oligosaccharides Using Fast-Atom-Bombardment Mass-Spectrometry," R.J.
`Linhardt, H.M. Wang, D. Loganathan, D. Lamb, L.M. Mallis, Carbohydrate Research, 225, 137-145, 1992.
`
`"Gradient Polyacrylamide Gel Electrophoresis for Determination of the Molecular Weights of Heparin Preparations and
`Low-Molecular-Weight Heparin Derivatives," R.E. Edens, A. Al-Hakim, J.M. Weiler, D.G. Rethwisch, J. Fareed, R.J.
`Linhardt, Journal of Pharmaceutical Sciences, 81, 823-827, 1992.
`
`"Isolation and Characterization of Human Heparin," R.J. Linhardt, S.A. Ampofo, J. Fareed, D. Hoppensteadt, J. B. Mulliken,
`J. Folkman, Biochemistry, 31, 12441-12445, 1992.
`
`"Search for the Heparin Antithrombin III-Binding Site Precursor," R.J. Linhardt, H.M. Wang, D. Loganathan, J.H. Bae, The
`Journal of Biological Chemistry, 267, 2380-2387, 1992.
`
`"New Methodologies in Heparin Structure Analysis and the Generation of LMW Heparins," R.J. Linhardt, H.M. Wang, S.A.
`Ampofo, in Heparin and Related Polysaccharides, Plenum Press, New York, D. Lane and U. Lindahl (Eds.), pp. 37-47,
`1992.
`
`"An Electrophoresis-Based Assay For Glycosyltransferase Activity," K.B. Lee, U.R. Desai, M.M. Palcic, O. Hindsgaul, R.J.
`Linhardt, Analytical Biochemistry, 205, 108-114, 1992.
`
`"Lectin Affinity Electrophoresis for the Separation of Fluorescently Labeled Sugar Derivatives," K.B. Lee, Y.S. Kim, R.J.
`Linhardt, Analytical Biochemistry, 203, 206-210, 1992.
`
`"Biodegradable Polymers for the Controlled Delivery of Vaccines," E. Schmitt, D.R. Flanagan, R.J. Linhardt, Proceedings of
`the ACS Division of Polymeric Materials: Science and Engineering, 66, 493-494, 1992.
`
`"Fundamental Studies on the Hydration, Erosion and Release Properties of Biodegradable Poly(esters)," E. Schmitt, D.R.
`Flanagan, R.J. Linhardt, Proceedings of the ACS Division of Polymeric Materials: Science and Engineering, 66, 1992.
`
`"Heparin and Modified Heparin Inhibit Complement Activation In Vivo," J.M. Weiler, R.E. Edens, R.J. Linhardt, D.P.
`Kapelanski, Journal of Immunology, 148, 3210-3215, 1992.
`
`"Negative Ion Fast-Atom Bombardment Tandem Mass Spectrometry to Determine Sulfate and Linkage Position in
`Glycosaminoglycan-Derived Disaccharides," D.J. Lamb, H.M. Wang, L.M. Mallis, R.J. Linhardt, Journal of the American
`Society for Mass Spectrometry, 3, 797-803, 1992.
`
`"Purification and Characterization of Heparin Lyases from Flavobacterium heparinum," D.L. Lohse, R.J. Linhardt, Journal
`of Biological Chemistry, 267, 24347-24355, 1992.
`
`"Biocatalytic Synthesis of Sugar-Containing Poly(acrylate)-Based Hydrogels," B.D. Martin, S.A. Ampofo, R.J. Linhardt, J.S.
`Dordick, Macromolecules, 25, 7081-7085, 1992.
`
`"Physical Stabilization of Acidic Fibroblast Growth Factor by Polyanions," D.B. Volkin, P.K. Tsai, J.M. Dabora, J.O. Gress,
`C.J. Burke, R.J. Linhardt, C.R. Middaugh, Archives in Biochemistry and Biophysics, 300, 30-41, 1993.
`
`"Degradation and Release Properties of Pellets Fabricated From Three Commercial Poly (D,L-Lactide-co-Glycolide)
`Biodegradable Polymers," E.A. Schmitt, D.R. Flanagan, R.J. Linhardt, Journal of Pharmaceutical Sciences, 82, 326-329,
`1993.
`
`"Nature of the Interaction of Heparin with Acidic Fibroblast Growth Factor," H. Mach, D.B. Volkin, C.J. Burke, C.R.
`Middaugh, R.J. Linhardt, J. Fromm, D. Loganathan, L. Mattsson, Biochemistry, 32, 5480-5489, 1993.
`
`0006
`
`

`
`99.
`
`
`100.
`
`
`101.
`
`
`102.
`
`
`103.
`
`
`104.
`
`
`105.
`
`
`106.
`
`
`107.
`
`
`108.
`
`
`109.
`
`
`110.
`
`
`111.
`
`
`112.
`
`
`113.
`
`
`114.
`
`
`115.
`
`
`116.
`
`
`117.
`
`
`118.
`
`
`119.
`
`
`
`"Structure Elucidation of a Novel Acidic Tetrasaccharide and Hexasaccharide Derived From a Chemically Modified
`Heparin," U.R. Desai, H.M. Wang, T.R. Kelly, R.J. Linhardt, Carbohydrate Research, 241, 249-259, 1993.
`
`"Heparin Is Not Just an Anticoagulant Anymore: Six and One-Half Decades of Studies on the Ability of Heparin to Regulate
`Complement Activation," R.E. Edens, R. J. Linhardt, Weiler, J.M., Complement Profiles, 1, 96-120, 1993.
`
`"Monoclonal Antibodies Prepared Against Heparin Lyase I and their Reactivity Towards Heparin Lyases I, II and III," K.
`Gu., R.E. Edens, K.A. Jandik, R.J. Linhardt, International Journal of Biochemistry, 25, 331-336, 1993.
`
`"Comparison of the Activity of Two Chondroitin AC Lyases on Dermatan Sulfate," K. Gu, J. Liu, A. Pervin, R.J. Linhardt,
`Carbohydrate Research, 244, 369-377, 1993.
`
`"Mapping and Sequencing of Oligosaccharides by Electrophoresis" R.J. Linhardt, J. Liu, X.-J. Han, Trends in Glycoscience
`and Glycotechnology, 5, 181-192, 1993.
`
`"Heparin Oligosaccharides Bind L- and P-Selectin and Inhibit Acute Inflammation," R.M. Nelson, O. Cecconi, W.G.
`Roberts, A. Aruffo, R.J. Linhardt, M.P. Bevilacqua, Blood, 82, 3253-3258, 1993.
`
`"Oligosaccharide Composition of Heparin and Low-Molecular-Weight Heparins by Capillary Electrophoresis," U.R. Desai,
`H.M. Wang, S.A. Ampofo, R.J. Linhardt, Analytical Biochemistry, 213, 120-127, 1993.
`
`"Specificity Studies on the Heparin Lyases from Flavobacterium heparinum," U.R. Desai, H.M. Wang, R.J. Linhardt,
`Biochemistry, 32, 8140-8145, 1993.
`
`"Substrate Specificity of the Heparin Lyases from Flavobacterium heparinum," U.R. Desai, H.M. Wang, R.J. Linhardt,
`Archives of Biochemistry and Biophysics, 306, 461-468, 1993.
`
`"Capillary Electrophoresis to Measure Sulfoesterase Activity on Chondroitin Sulfate and Heparin Derived Disaccharides" A.
`Pervin, K. Gu, R.J. Linhardt, Applied and Theoretical Electrophoresis, 3, 297-303, 1993.
`
`"Heparin and Derivatized Heparin Inhibit Zymosan and Cobra Venom Factor Activation of Complement in Serum," R.E.
`Edens, R.J. Linhardt, C. S. Bell, J.M. Weiler, Immunopharmacology, 27, 145-153, 1994.
`
`"Biodegradable Polymers for the Controlled Delivery of Vaccines," E. Schmitt, D.R. Flanagan, R.J. Linhardt, in
`Biotechnology and Bioactive Polymers, Plenum Publishing Company, New York, C. Gebelein and C. Carraher (Eds.), pp
`249-258, 1994.
`
`"Analysis of Glycosaminoglycans with Polysaccharide Lyases," R.J. Linhardt, in Current Protocols in Molecular Biology,
`Analysis of Glycoconjugates, A. Varki (ed.), V 2, 17.13.17-17.13.32, Wiley Interscience, Boston, 1994.
`
`"Molecular Weight of Low Molecular Weight Heparins by 13C Nuclear Magnetic Resonance Spectroscopy," U.R. Desai, R.J.
`Linhardt, Carbohydrate Research, 255, 193-212, 1994.
`
`"Low Molecular Weight Dermatan Sulfate as an Antithrombotic Agent: Structure-Activity Relationship Studies" R.J.
`Linhardt, U.R. Desai, J. Liu, A. Pervin, D. Hoppensteadt, J. Fareed, Biochemical Pharmacology, 47, 1241-1252, 1994.
`
`"Interaction of Heparin with Synthetic Antithrombin III Peptide Analogues," J. Bae, U.R. Desai, A. Pervin, E.E.O. Caldwell,
`J.M. Weiler, R.J. Linhardt, Biochemical Journal, 301, 121-129, 1994. PMID: 8037658, PMCID: PMC1137151
`
`"Capillary Electrophoresis of Oligosaccharides" R.J. Linhardt, in Methods in Enzymology, Guide to Techniques in
`Glycobiology, W.J. Lennarz, G.W. Hart (Eds.), Volume 230, Chapter 16, pp 265-280, Academic Press, San Diego, 1994.
`
`"Importance of Distinct Water Environments in the Hydrolysis of Poly(D,L-lactide-co-glycolide)," E.A. Schmitt, D.R.
`Flanagan, R.J. Linhardt, Macromolecules, 27, 743-748, 1994.
`
`"Action Pattern of Polysaccharide Lyases on Glycosaminoglycans" K.A. Jandik, K. Gu, R.J. Linhardt, Glycobiology, 4, 289-
`296, 1994.
`
`"Accelerated Degradation of Poly(ε-caprolactone) by Organic Amines," W.-J. Lin, D.R. Flanagan, R.J. Linhardt,
`Pharmaceutical Research, 11, 1030-1034, 1994.
`
`"New Approaches for the Preparation of Hydrophobic Heparin Derivatives," J. Liu, A. Pervin, C.M. Gallo, U.R. Desai, C.L.
`Van Gorp, R.J. Linhardt, Journal of Pharmaceutical Science, 83, 1034-1039, 1994.
`
`0007
`
`

`
`"Chemical and Biochemical Catalysis to make Swellable Polymers," J.S. Dordick, R.J. Linhardt, D.G. Rethwisch Chemtech,
`24, 33-39, 1994.
`
`"Separation of Glycosaminoglycan-Derived Oligosaccharides by Capillary Electrophoresis Using Reverse Polarity," A.
`Pervin, A. Al-Hakim, R.J. Linhardt, Analytical Biochemistry, 221, 182-188, 1994.
`
`"Directional Immobilization of Heparin onto Beaded Supports," V. D. Nadkarni, A. Pervin, R.J. Linhardt, Analytical
`Biochemistry, 222, 59-67, 1994.
`
`"Human Milk Glycosaminoglycans Inhibit HIV Glycoprotein gp120 Binding to Its Host Cell CD4 Receptor " D.S. Newburg,
`R.J. Linhardt, S.A. Ampofo, R.H. Yolken, Journal of Nutrition, 125, 419-424, 1995.
`
`"IGFBP-3 and IGFBP-5 Association with Endothelial Cells: Role of C-terminal Heparin Binding Domain," B.A. Booth, M.
`Boes, D.L. Andress, B.L. Dake, M.C. Kiefer, C. Maack, R.J. Linhardt, K. Bar, E.E.O. Caldwell, J. Weiler, R. S. Bar,
`Growth Regulation, 5, 1-17, 1995.
`
`"Preparation and Structural Characterization of Large Heparin-Derived Oligosaccharides," A. Pervin, C. Gallo, K. A. Jandik,
`X.-J. Han, R.J. Linhardt, Glycobiology, 5, 83-95, 1995.
`
`"Analysis of Fluorescently Labeled Sugars by Reversed-Phase Ion-Pairing High-Performance Liquid Chromatography," Y.S.
`Kim, J. Liu, X.J. Han, A. Pervin, R.J. Linhardt, Journal of Chromatographic Science, 33, 162-167 1995.
`
`"Capillary Electrophoresis for Monitoring Chemical Reactions: Sulfation and Synthetic Manipulation of Sulfated
`Carbohydrates," R.J. Kerns, I.R. Vlahov, R.J. Linhardt, Carbohydrate Research, 267, 143-152, 1995.
`
`"Lectin Affinity Electrophoresis," R.J. Linhardt,