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`5847354
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`12526296
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`International Application Number:
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`PCT/US08/01602
`
`Confirmation Number:
`
`5197
`
`Title of Invention:
`
`NRF2 SCREENING ASSAYS AND RELATED METHODS AND COMPOSITIONS
`
`First Named Inventor/Applicant Name:
`
`Matvey E LUKASHEV
`
`Customer Number:
`
`2285 2
`
`Filer:
`
`Richard V. Burgujian/ROSIE CREMEANS
`
`Filer Authorized By:
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`Richard V. Burg ujian
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`08201.0077
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`07-AUG-2009
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`Biogen Exhibit 2382
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`Coalition v. Biogen
`IPR2015-01993
`
`Page 1 of 46
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`Biogen Exhibit 2382
`Coalition v. Biogen
`IPR2015-01993
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`

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`SPEC—DRAW.pdf
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`NPL Documents
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`This Acknowledgement Receipt evidences receipt on the noted date by the USPTO of the indicated documents,
`characterized by the applicant, and including page counts, where applicable. It serves as evidence of receipt similar to a
`Post Card, as described in MPEP 503.
`
`New Applications Under 35 U.S.C. 111
`lfa new application is being filed and the application includes the necessary components for a filing date (see 37 CFR
`1.53(b)-(d) and MPEP 506), a Filing Receipt (37 CFR 1.54) will be issued in due course and the date shown on this
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`.3
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`(I2) IN'l‘I£RNA'I'IONAL AI’I’LICA'I'ION PUBLISHED UNDER THE I’A'l'EN'l‘ CO()I’l£I{ATION 'l‘RI£A'l'Y
`
`(I’C'I')
`
`(19) World Intellectual Property Organization
`International Bureau
`
`(43) International Publication Date
`14 August 2008 (14.08.2008)
`
`international Patent Classification:
`GWN 33/50 (2006.01)
`
`International Application Number:
`PCTfUS2003/00 l 602
`
`International Filing Date: 7 February 2008 (07.02.2008)
`
`Filing Language:
`
`Publication Language:
`Priority Data:
`00/888,921
`
`English
`
`English
`
`8 February 2()07 (08.02.2007)
`
`US
`
`(10) International Publication Number
`WO 2008/097596 A2
`
`(81) Designated States (unlexr otherwire intlimted. for every
`kind ufnational protection available): AE, AG, AL, AM,
`A0, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA,
`CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC. EE,
`EG, ES, FI, GB, GD. GE, GH, GM, GT, HN, HR, HU, ID.
`IL. IN, IS, JP, KE, KG, KM, KN, KP, KR. KZ, LA, LC,
`LK, LR, LS, LT, LU, LY, MA, MD, ME. MG. MK, MN,
`MW, MX. MY. MZ, NA, NG, NI, NO, NZ, OM. PG. PH,
`PL, PT, RO, RS, RU. SC, SD, Sli, SG, SK, SL. SM, SV,
`SY, TI, TM. TN, TR, TT, TZ, UA. UG, US, UZ, VC, VN,
`ZA, ZM, ZW.
`
`Appllcant (_/or all designated States except US): BIOGEN
`[DEC MA INC. [USIUS]; 14 Cambridge Center. Cam—
`bridge, MA 0214'.’ (US).
`
`Inventor; and
`LUKASHEV,
`(for US only):
`Inventor/Applicant
`Matvey, E. [US/US]; 3 Louis Road, Tewlcsbury, MA
`01876 (US).
`
`Agent: GARRE’l'T, Arthur, S.; Finnegan, Henderson,
`Fambuw, Garrett & Dunner L.L.P., 901 New York Avenue,
`NW, Washington, DC 0001-44I3 (US).
`
`Designated States (unler: otherwire intlicaled. for every
`kind of regional protection available): ARIPO (BW, GI I,
`GM, KE, IS, MW, M7,, NA, SD, SL, SZ. 17.. UG, ’/.M.
`ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ. TM).
`European (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI,
`FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV. MC, MT, NL,
`NO, PL, PT, RO, SE, SI, SK, TR), OAPI (BF, BJ, CF, CG,
`CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
`Published:
`
`without inlemational retm-h report and to be republished
`upon receipt of that report
`
`(51)
`
`421)
`
`(Z2)
`
`(Z5)
`
`(26)
`
`(30)
`
`(71)
`
`llIlllllllllllllllllllllllllllllllllllllllllllllll
`
`N < \
`
`:>
`ax
`In
`I\
`as
`cK
`oo
`
`oo 6
`
`_____._____._______________.._.__j________.._____
`(54) Title: NRF2 SCREENING ASSAYS AND RELATED METHODS AND COMPOSITIONS
`
`' (57) Abstract:
`Provided are certain methods of screening, identifying, and evaluating neuroprutcctivc compounds useful for treat-
`ment of neurological diseases, such as, e.g.. multiple sclerosis (MS). The compounds described upregulate the cellular cytoprotective
`' g such compounds in therapy for neurological disease, par—
`ticularly, for slowing or reducing demyelination, axonal loss. or ne
`uronal and oligodendrocyte death.
`
`3
`
`Page 5 of 46
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`

`

`VVO 2008/097596
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`PCT/US2008/001602
`
` 9
`
`Nrf2 SCREENING ASSAYS
`
`AND RELATED METHODS AND COMPOSITIONS
`
`[0001]
`
`Provided are certain compounds for treating neurological diseases,
`
`including demyelinating neurological diseases, such as, e.g.. multiple sclerosis.
`
`[0002]
`
`Multiple sclerosis (MS) is an autoimmune disease with the autoimmune
`
`activity directed against central nervous system (CNS) antigens. The disease is
`
`characterized by inflammation in parts of the CNS. leading to the loss of the myelin
`sheathing around neuronal axons (demyelination), loss of axons, and the eventual
`
`death of neurons, oligodenrocytes and glial cells.
`
`[0003] An estimated 2,500,000 people in the world suffer from MS.
`
`it is one of
`
`the most common diseases of the CNS in young adults. MS is a chronic, progressing,
`disabling disease. which generally strikes its victims some time after adolescence, with
`diagnosis generally made between 20 and 40 years of age, although onset may occur
`earlier. The disease is not directly hereditary, although genetic susceptibility plays a
`part in its development. Relapsing-remitting MS presents in the form of recurrent
`
`attacks of focal or multifocal neurologic dysfunction. Attacks may occur, remit, and
`
`recur, seemingly randomly over many years. Remission is often incomplete and as
`one attack follows another, a stepwise downward progression ensues with increasing
`pennanent neurological deficit.
`
`[0004] Although various immunotherapeutic drugs can provide relief in
`
`patients with MS. none is capable of reversing disease progression, and some can
`
`cause serious adverse effects. Most current therapies for MS are aimed at the
`
`reduction of inflammation and suppression or modulation of the immune system. As of
`2006. the available treatments for MS reduce inflammation and the number of new
`
`episodes but not all have an effect on disease progression. A number of clinical trials
`have shown that the suppression of inflammation in chronic MS rarely significantly
`limits the accumulation of disability through sustained disease progression, suggesting
`that neuronal damage and inflammation are independent pathologies. Promoting CNS
`remyelination as a repair mechanism and othenivise preventing axonal loss and
`
`Page 6 of 46
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`

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`WO 2008/097596
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`PCT/US2008/001602
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`neuronal death are some of the important goals for the treatment of MS. For a
`comprehensive review of MS and its current therapies, see. e.g.. McAlpine's Multiple
`Sclerosis, by Alastair Compston et al., 4th edition. Churchill Livingstone Elsevier.
`2006.
`
`[0005]
`
`"Phase 2 enzymes" serve as a protection mechanism in mammalian
`
`cells against oxygen/nitrogen species (ROS/RNS), electrophiles and xenobiotics.
`These enzymes are not normally expressed at their maximal levels and. their
`expression can be induced by a variety of natural and synthetic agents. Nuclear factor
`E2-related factor 2 (Nrf2) is a transcription factor responsible for the induction of a
`variety of important antioxidant and detoxification enzymes that coordinate a protective
`cellular response to metabolic and toxic stress.
`
`[0006] ROS/RNS are most damaging in the brain and neuronal tissue. where
`they attack post-mitotic (i.e., non-dividing) cells such as glial cells. oligodendocytes.
`and neurons. which are particularly sensitive to free radicals. This process leads to
`neuronal damage. Oxidative stress has been implicated in the pathogenesis of a
`variety of neurodegenerative diseases, including ALS. Alzheimers disease (AD). and
`Parkinson's disease (PD). For review, see, e.g.. van Muiswinkel et al., Curr. Drug
`Targets CNS--Neurol. Disord.. 2005, 4:267-281. An anti-oxidative enzyme under
`control of Nrf2. NQO1 (NAD(P)H dehydrogenase. quinone (1), was recently reported
`to be substantially upregulated in the brain tissues of AD and PD subjects (Muiswinkel
`et al., Neurobiol. Aging, 2004, 25: 1253). Similarly, increased expression of NQO1
`was reported in the ALS subjects’ spinal cord (Muiswinkel et al., Curr. Drug
`Targets-CNS. Neurol. Disord.. 2005, 4267-281) and in active and chronic lesions in
`the brains of patients suffering from MS (van Horssen et al.. Free Radical Biol. & Med.,
`2006. 41 311-311). These observations indicate that the Nrf2 pathway may be
`activated in neurodegenerative and neuroinflammatory diseases as an endogenous
`protective mechanism.
`indeed. most recently. it has been reported that induced
`activation of Nrf2-dependent genes by certain cyclopenanone-based compounds
`(NEPP) counters the toxic effects of metabolic inhibition and ROS/RNS production in
`
`Page 7 of 46
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`WO 2008/097596
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`PCT/US2008/I10 1602
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`the brain and protects neurons from death in vitro and in vivo (see Satoh et al., PNAS,
`2006, 103(3):768-773).
`
`[0007] Additionally, many publications have reported neuroprotective effects of
`
`compounds in natural plant-derived compounds (“phytochemicals"), including
`
`a-tocopherol (vitamin E), lycopene (tomatoes), resveratrol (red grapes), sulforaphane
`(broccoli), EGCG (green tea). etc. For review, see Mattson and Cheng, Trends in
`
`Neurosci., 2006. 29(11):632-639. Originally, the action of these compounds was
`
`attributed to their anti-oxidant properties. However. while most anti-oxidants are
`
`effective only at high concentrations, at least some of these compounds appear to
`
`exert neuroprotective effects at much lower doses. Emerging evidence suggests that
`
`these compounds may exert their neuroprotective effects by activating cellular
`
`stress-response pathways, including the Nrf2 pathway, resulting in the upregulation of
`
`neuroprotective genes. However, the exact mechanism of action of these compounds
`remains poorly understood.
`
`[0008] To date. more than 10 different chemical classes of inducers of Nrf2
`
`pathway have been identified including isothiocyanates and their thiol addition
`
`products. dithiocarbamates. as well as 1,2-dithiole-3-thiones, trivalent arsenic
`
`derivatives (e.g., phenyl arsenoxide), heavy metals, certain conjugated cyclic and
`
`acyclic polyenes (including porphyrins, chlorophyllins, and chlorophyll), and vicinal
`dimercaptans. These inducers have few structural similarities. They are mostly
`electrophiles, and all can react chemically with thiol groups by alkylation, oxidation, or
`
`reduction, suggesting that the intracellular sensor for inducers is likely to contain very
`highly reactive (cysteine) thiols. The inducers can modify thiol groups by a variety of
`mechanisms including: alkylation (Michael addition acceptors, isothiocyanates,
`
`quinones); oxidation (e.g., peroxides and hydroperoxides); and direct reaction with
`
`thiol/disulfide linkages (e.g., vicinal dithiols such as 1 .2-dimercaptopropanol, lipoic
`
`acid). These diverse response mechanisms provide plasticity for cellular responses to
`
`a variety of electrophilic and oxidant stressors.
`
`[0009] Provided are methods that comprise at least one of the following
`methods:
`
`Page 8 of 46
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`

`

`W0 21108/097596
`
`,3
`
`PCTIUS2008/001602
`
`1) methods of screening for at least one new candidate compound for
`
`treating a neurological disease;
`
`2) methods of evaluating neuroprotective properties of at least one dmg
`
`candidate for treating a neurological disease;
`
`3) methods of comparing (e.g., for bioequivalence) at least two
`
`pharmaceutical compositions which comprise fumaric acid derivatives;
`
`4) methods of treating a neurological disease by administering to the subject
`
`in need thereof at least one compound that is partially structurally similar to
`DMF or MMF; and
`
`methods of treating a neurological disease by a combination therapy that
`
`comprises administration of at least one first compound that upregulates
`
`the Nrf2 pathway and at least one second compound that does not
`
`upregulate the Nrf2 pathway.
`
`[0010]
`
`In some embodiments, the neurological disease is a neurodegenerative
`
`disease such as, for example, ALS, Parkinson's disease, Alzheimer's disease, and
`
`Huntington's disease.
`
`In some embodiments the neurological disease is MS or
`
`another demyelinating neurological disease.
`
`[0011]
`
`In some embodiments, the methods 1-3 further comprise:
`
`a) contacting a cell with the test compound. and
`
`b) determining whether the Nrf2 pathway is upregulated in the cell.
`
`In some embodiments, the methods may further comprise:
`
`c) detennining whether the test compound slows or prevents demyelination.
`
`axonal loss, and/or neuronal death, and/or
`
`d) selecting the test compound as a candidate for treating neurodegeneration
`
`in a neurological disease if 1) the Nrf2 pathway is upregulated and 2)
`
`demyelination. axonal loss. and/or neuronal death are/is prevented or
`slowed.
`
`[0012]
`
`In some embodiments. the methods 1-3 comprise contacting a cell with
`
`at least one test compound and determining whether the Nrf2 pathway is upregulated
`in the cell.
`In such methods. an upregulation of the Nrf2 pathway above a threshold
`
`Page 9 of 46
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`W0 2008/097596
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`PCT/US2008/001602
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`(e.g., by at least 30% over a control) indicates that the at least one compound has at
`
`least one biological property beneficial in treating a neurological disease (e.g.,
`
`neuroprotective properties).
`
`In some embodiments, the upregulation of the Nrf2
`
`pathway is assessed (in vivo and/or in vitro) by at least one of the following:
`
`i) expression levels of endogenously produced and/or exogenously
`introduced Nrf2;
`
`ii) subcellular localization and/or nuclear translocation of Nrf2;
`
`iii) expression levels and/or activity of one or more genes under control of
`
`Nrf2 (e.g., endogenous NQO1) or an Nrf2-regulated reporter gene in an
`
`artificial reporter constmct;
`
`levels of Nrf2 binding to the Nrf2-binding DNA element ARE;
`
`stability of Nrf2/Keap1 complexes; and
`
`modification (e.g., alkylation) levels of Keap1 and/or at least one other
`
`Nrf2/Keap1-associated proteins.
`
`[0013]
`
`In some embodiments of methods 1-3, the compounds that are being
`
`screened. evaluated. or compared comprise at least one member of at least one of the
`
`following classes of compounds: mild alkylating agents, Michael addition acceptors,
`
`and compounds that are metabolized upon administration to Michael addition
`
`in some embodiments, the Michael addition acceptor has the structure of
`acceptors.
`Formula I, ll, ill. or IV set forth below.
`
`[0014]
`
`in some embodiments method 1 comprises:
`
`a) contacting a cell with a plurality of test compounds.
`
`b) determining whether the Nrf2 pathway is upregulated in the cell. and
`
`c) selecting from the plurality of compounds at least one compound that
`
`upregulates the Nrf2 pathway,
`
`wherein an upregulation of the Nrf2 pathway by the selected at least one compound
`
`indicates that the selected at least one compound may be useful for treating a
`
`neurological disease. The plurality of compounds may be represented, e.g.. by a
`
`combinatorial chemical library, and the method may be performed. e.g.. by
`
`high-throughput screening.
`
`Page 10 of 46
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`[0015]
`
`in some embodiments method 2 comprises:
`
`a) contacting a cell with the at least one drug or dmg candidate. and
`
`b) determining whether the Nrf2 pathway is upregulated in the cell,
`
`wherein an upregulation of the Nrf2 pathway by the at least one drug or drug
`candidate indicates that the at least one drug or drug candidate is useful for
`
`neuroprotection in treating a human having a neurological disease.
`
`[0016]
`
`In some embodiments method 3 comprises:
`
`a) contacting a cell with a first composition comprising at least one test
`compound. and
`
`b) comparing the level of Nrf2 pathway upregulation in the cell by the at least
`
`one test compound to the corresponding level of the Nrf2 pathway
`upregulation in a control cell treated with a second composition comprising
`at least one of DMF and MMF.
`
`In some embodiments of method 3, the test compound is fumaric acid,
`[0017]
`a salt thereof, or a fumaric acid derivative.
`In some embodiments, the first
`
`composition comprises DMF. MMF. or both.
`
`In some embodiments, the dose and/or
`
`the formulation of the first composition differs from the dose and/or the formulation of
`
`the second composition.
`
`[0018]
`In some embodiments, method 3 further comprises:
`c) comparing at least one phannacokinetic parameter (e.g., serum-half-life) of
`the first and the second compositions.
`
`In some embodiments method 4 comprises administering to the
`[0019]
`mammal a therapeutically effective amount of at least one neuroprotective compound
`having Fonnula I, ll, ill, or IV. e.g., a fumaric acid derivative (e.g., DMF or MMF).
`[0020]
`In some embodiments method 4 provides a method of slowing or
`preventing neurodegeneration in a patient in need thereof. by administering the
`compound in an amount and for a period of time sufficient to slow or prevent
`demyelination. axonal loss, and/or neuronal death. e.g., by at least 30% relative to a
`control.
`
`[0021]
`
`In some embodiments method 5 comprises:
`
`Page 11 of 46
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`WO 2008/097596
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`PCTlUS2008/00 1602
`
`a) administering to the mammal a therapeutically effective amount of at least
`
`one first compound that upregulates the Nrf2 pathway. and
`
`b) administering a therapeutically effective amount of at least one second
`
`compound that does not upregulate the Nrf2 pathway.
`
`In some embodiments of method 5, the at least one first compound’,
`[0022]
`used in step (a), is a compound of Formula I, ll, Ill. or IV. e.g.. a fumaric acid derivative
`
`(e.g., DMF or MMF); and the at least one second compound, which is used in step (b).
`
`is an immunosuppressive or an immunomodulatory compound that does not
`
`upregulate the Nrf2 pathway (e.g.. by more than 30% over a control).
`
`[0023]
`
`In some embodiments method 5 comprises administering to the
`
`mammal a therapeutically effective amount of a compound of Fonnula I, ll. lll, or IV.
`
`[0024]
`
`in some embodiments of methods 1-5, the at least onecompound being
`
`screened, identified. evaluated, or used for treating a neurological disorder is not
`
`fumaric acid or its salt, or a fumaric acid derivative (e.g.. DMF or MMF).
`
`[0025] Other features and embodiments of the invention will be apparent from
`
`the following description and the claims.
`
`BRIEF DESCRIPTION OF THE FIGURES
`
`[0026] Figure 1 demonstrates that DMF and MMF are activators of Nrf2 at
`
`concentrations within clinical exposure range (cells in culture).
`
`[0027] Figure 2 shows results of RNAi experiments.
`
`[0028] Figure 3 shows evidence of Nrf2 activation by DMF and MMF In vivo.
`
`[0029] Figure 4 shows evidence of Nrf2 activation by DMF and MMF In vivo.
`[0030] Fumaric acid esters, such as DMF. have been proposed for treatment
`
`of MS (see, e.g.. Schimrigk et al., Eur. J. Neurol.. 2006, 13(6):604-10; Drugs R&D.
`2005, 6(4):229-30).
`
`[0031] Provided are, among other things, means for identifying compounds
`
`with a new therapeutic modality useful in at least one of multiple neurological
`
`indications and. optionally, complementary to other drugs for the treatment of a
`
`neurological disease. including a number of currently used immunomodulators.
`
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`[0032] DMF is a member of a large group of anti-oxidant molecules known for
`
`their cytoprotective and anti-inflammatory properties. These molecules also share the
`
`property of the Nrf2 pathway activation. Thus, the finding that DMF activates the Nrf2
`
`pathway in conjunction with the neuroprotective effects of DMF further offers a
`
`rationale for identification of structurally and/or mechanistically related molecules that
`
`would be expected to be therapeutically effective for the treatment of neurological
`
`disorders, such as, e.g., MS.
`
`[0033] Certain terms are defined in this section; additional definitions are
`
`provided throughout the description.
`
`[0034] The terms "activation" and "upregulation," when used in reference to
`
`the Nrf2 pathway, are used interchangeably herein.
`
`[0035] The terms "disease" and "disorder" are used interchangeably herein.
`
`[0036] The term "a drug for treating a neurological disease" refers to a
`
`compound that has a therapeutic benefit in a specified neurological disease as shown
`
`in at least one animal model of a neurological disease or in human clinical trials for the
`
`treatment of a neurological disease.
`
`[0037] The tenn “neuroprotection” and its cognates refer to prevention or a
`
`slowing in neuronal degeneration, including, for example, demyelination andlor axonal
`
`loss, and/or, neuronal and/or oligodendrocyte death. Neuroprotection may occur
`
`through several mechanisms, e.g., through reducing inflammation, providing
`
`neurotrophic factors, scavenging free radicals, etc. As used herein, a compound is
`
`considered neuroprotective if it (1) upregulates the Nrf2 pathway above a certain
`
`threshold and (2) provides neuroprotection, regardless of possible other mechanisms
`of action.
`
`[0038] The terms “treatment," “therapeutic method," "therapeutic benefits," and
`
`the like refer to therapeutic as well as prophylactic/preventative measures. Thus,
`
`those in need of treatment may include individuals already having a specified disease
`
`and those who are at risk for acquiring that disease.
`
`[0039] The terms “therapeutically effective dose" and “therapeutically effective
`
`amount" refer to that amount of a compound which results in at least one of prevention
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`or delay of onset or amelioration of symptoms of a neurological disorder in a subject or
`
`an attainment of a desired biological outcome, such as reduced neurodegeneration
`
`(e.g., demyelination, axonal loss. and neuronal death) or reduced inflammation of the
`cells of the CNS.
`
`[0040]
`
`In one aspect, provided are methods of evaluating neuroprotective
`
`properties of test compounds, including the following methods:
`
`1) methods of screening for new candidate compounds that may be
`
`useful for treating a neurological disease;
`
`2) methods of evaluating neuroprotective properties of drugs and
`
`candidates that are used or proposed for treating a neurological
`disease;
`
`3) methods of comparing (e.g., for bioequivalence) two or more
`
`phannaceutical compositions which contain fumaric acid
`
`derivatives;
`
`[0041]
`
`In some embodiments. methods 1-3 may comprise:
`
`a) contacting a cell with the test compound.
`
`b) determining whether the Nrf2 pathway is upregulated in the cell,
`
`and, in some embodiments. additionally performing the following step(s):
`
`c) detennining whether the test compound slows or prevents demyelination,
`
`axonal loss, and/or neuronal death. and/or
`
`d) selecting the test compound as a candidate for treating neurodegeneration
`
`in a neurological disease if 1) the Nrf2 pathway is upregulated and 2)
`
`demyelination, axonal loss, and/or neuronal death are/is prevented or
`slowed.
`
`Method 1
`
`[0042]
`
`In some embodiments the methods of screening for a candidate
`
`compound for treating a neurological disease comprise:
`
`a) contacting a cell with a plurality of test compounds,
`
`b) determining whether the Nrf2 pathway is upregulated in the cell, and
`
`c) selecting from the plurality of compounds at least one compound that
`
`upregulates the Nrf2 pathway,
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`wherein an upregulation of the Nrf2 pathway by the selected at least one compound
`
`indicates that the selected at least one compound may be useful for treating a
`
`neurological disease. For example, the plurality of compounds may be represented by
`
`a combinatorial chemical library, and the screening method may be performed by a
`
`high—throughput screening as described in, e.g.. High-Throughput Screening in Drug
`
`Discovery (Methods and Principles in Medicinal Chemistry), by Jorg Hiiser (ed.), John
`
`Wiley & Sons (2006).
`
`[0043] Combinatorial libraries of compounds are also described in, e.g..
`
`Solid-Supported Combinatorial and Parallel Synthesis of Small-Molecular~Weight
`
`Compound Libraries (Tetrahedron Organic Chemistry) Ian Salusbury (ed.), Elsevier
`
`(1998); Combinatorial Libraries: Synthesis, Screening and Application Potential
`
`(Library Binding), by Riccardo Cortese (ed.). Walter de Gruyter (1995). The libraries of
`
`compounds may be. for example. quinone libraries and other libraries as described in
`
`Mittoo, Comb. Chem. & High Throughput Screen, 2006, 9:421-423.
`
`[0044]
`
`In some embodiments, the at least one compound or plurality of
`
`compounds being screened and/or selected comprises at least one compound
`
`selected from at least one of the following groups of compounds: mild alkylating
`
`agents, Michael addition acceptors or compounds that are metabolized to Michael
`
`addition acceptors, including compounds of Formulas I, II, III, or IV.
`
`[0045]
`
`in some of the embodiments, the at least one compound is selected
`
`from fumaric acid, its salts, and fumaric acid derivatives.
`
`Method 2
`
`[0046] Also provided are methods of evaluating neuroprotective properties of
`
`at least one dmg or drug candidate for treating at least one neurological disease.
`
`Such methods comprise:
`
`a) contacting a cell with the at least one drug or drug candidate, and
`
`b) determining whether the Nrf2 pathway is upregulated in the cell,
`
`wherein the upregulation of the Nrf2 pathway by the at least one drug or drug
`
`candidate indicates that the at least one drug or drug candidate is neuroprotective in
`
`treating a human having a neurological disease.
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`[0047]
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`in some embodiments, the upregulation of the Nrf2 pathway by the at
`
`least one drug or drug candidate indicates that the at least one drug or drug candidate
`
`has at least one activity selected from slowing demyelination, slowing the loss of
`
`axons, and slowing the rate of neuronal death.
`
`[0048]
`
`in some embodiments. the method of evaluating at least one drug or
`
`drug candidate comprises an additional step:
`
`c) evaluating demyelination, loss of axons, andlor neuronal death.
`
`[0049]
`
`In some embodiments. steps a) and c) are perfonned in vivo in at least
`
`one model of a neurological disease, e.g.. as described below.
`
`[0050]
`
`In other embodiments. particularly those in which the neurological
`
`disease is multiple sclerosis or another demyelinating disease. the evaluated at least
`
`one dmg or drug candidate for a neurological disease is chosen from the following:
`
`FTY720 (2-(4-octylphenethyl)-2-aminopropane-1.3-diol; Novartis); anti-lL12 antibody
`
`(e.g., ABT-874: Abbott Laboratories); GSK683699 (GSKlTanabe); Neurovax (Immune
`
`Response Corp.; Darlington. Curr. Opin. Mol. Ther., 2005, 7(6):598-603); anti-CCR2
`
`antibody (e.g., MLN 1202; Millennium); interferon B-1a (e.g., Avonex®; Biogen ldec);
`
`anti-a4-integrin antibody (e.g., Tysabri®; Biogen ldec/Elan); anti-CD20 antibody (e.g.,
`
`Rituxan® (Biogen |declGenentech); TV 5010 (Teva); NBI-788 (Neurocrine); MBPB298
`
`(BioMS (see Warren et al.. Eur. J. Neurol., 2006, 13(8):887-95); Mylinax (Oral
`
`Cladribine; 2-chlorodeoxyadenosine; Serono/IVAX); Teriflunomide
`
`((Z)-2-cyano-N-(4-(trifluoromethyl)phenyl)-3-hydroxybut-2-enamide; Sanofi-Aventis);
`
`Temsirolimus (Wyeth); Laquinimod
`
`(5-chloro-N-ethyl-1 .2-dihydro—4-hydroxy-1-methyl-2-oxo-N-phenylquinoline-3-carboxa
`
`mide; Active Biotech/Teva); and interferon tau (Tauferon; Pepgen).
`
`[0051]
`
`In some embodiments, the at least one drug or drug candidate being
`
`evaluated is at least one compound selected from at least one class selected from a
`
`mild alkylating agent, a Michael addition acceptor, and a compound that is
`
`metabolized to a Michael addition acceptor, including compounds of Formulas I, ll. Ill.
`or IV.
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`In some of the embodiments, the compound is fumaric acid, its salt. or '
`[0052]
`a fumaric acid derivative.
`
`Method 3
`
`[0053] Also provided are methods of comparing (e.g., for bioequivalence) at
`
`least two pharmaceutical compositions. Such methods comprise:
`
`a) contacting a cell with at least one first composition comprising a test
`compound, and
`
`b) comparing the level of the Nrf2 pathway upregulation in the cell by the test
`
`compound to the corresponding level of the Nrf2 pathway upregulation in a
`
`cell treated with at least one second composition (“comparator
`
`composition") comprising DMF, MMF, or both.
`
`[0054]
`
`in some embodiments, substantially dissimilar levels of upregulation by
`
`the at least one first and at least one second compositions indicate that the
`
`compositions are not bioequivalent.
`
`[0055]
`
`In some embodiments, the test compound is fumaric acid, its salt
`
`thereof, a fumaric acid derivative, or mixtures thereof.
`
`in some embodiments, the first
`
`composition comprises at least one of DMF. MMF, and both DMF and MMF.
`
`ln some
`
`embodiments, the dose and/or the formulation of the at least one first composition
`
`differs from the dose and/or the formulation of the at least one second composition.
`
`The at least one first composition may be a controlled release composition such as.
`
`e.g., compositions described in WO 2006/037342.
`
`[0056]
`
`In some embodiments, the method further comprises and additional
`
`step:
`
`c) comparing at least one pharmacokinetic parameter of the at least one first
`
`and the at least one second compositions.
`
`[0057] Pharmacokinetic parameters and methods for evaluating the same are
`
`well known and are described in, e.g., Phannacokinetics, Second Edition (Drugs and
`
`the Phannaceutical Sciences) by Milo Gibaldi et al. (eds.), lnforma Healthcare (1982).
`
`Examples of such pharmacokinetic parameters that can be evaluated include semm
`
`half-life, clearance, and volume distribution.
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`[0058]
`
`in some embodiments, substantially dissimilar pharrnacokinetic
`
`parameter(s) of the a least one first and at least one second compositions indicate that
`
`the compositions are not bioequivalent.
`
`[0059]
`
`In some embodiments, the test compound being evaluated is a mild
`
`alkylating agent, and more specifically. a Michael addition acceptor, or a compound
`
`that is metabolized to a Michael addition acceptor.
`
`[0060]
`
`In some of the embodiments. the test compound is fumaric acid or its
`
`salt. or a fumaric acid