Journal of Toxicology: Cutaneous and Ocular Toxicology
`
`ISSN: 0731-3829 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/icot19
`
`Evaluation of the Toxicity of Benzalkonium
`Chloride on the Ocular Surface
`
`CAROLINE DEBBASCH, PATRICE RAT, JEAN-MICHEL WARNET, MAGDA DE
`SAINT JEAN, CHRISTOPHE BAUDOUIN & Pisella Pierre-Jean
`
`To cite this article: CAROLINE DEBBASCH, PATRICE RAT, JEAN-MICHEL WARNET, MAGDA DE
`SAINT JEAN, CHRISTOPHE BAUDOUIN & Pisella Pierre-Jean (2000) Evaluation of the Toxicity of
`Benzalkonium Chloride on the Ocular Surface, Journal of Toxicology: Cutaneous and Ocular
`Toxicology, 19:2-3, 105-115, DOI: 10.3109/15569520009051506
`
`To link to this article: http://dx.doi.org/10.3109/15569520009051506
`
`Published online: 27 Sep 2008.
`
`Submit your article to this journal
`
`Article views: 44
`
`View related articles
`
`Citing articles: 1 View citing articles
`
`Full Terms & Conditions of access and use can be found at
`http://www.tandfonline.com/action/journalInformation?journalCode=icot19
`
`Download by: [Emily Florio]
`
`Date: 14 September 2015, At: 06:37
`
`SENJU EXHIBIT 2064
`LUPIN v SENJU
`IPR2015-01105
`
`PAGE 1 OF 12
`
`
`
`ISSN: 0731-3829 (Print) (Online) Journal homepage: http://www.tandfonline.com/loi/icot19
`
`Evaluation of the Toxicity of Benzalkonium
`Chloride on the Ocular Surface
`
`CAROLINE DEBBASCH, PATRICE RAT, JEAN-MICHEL WARNET, MAGDA DE
`SAINT JEAN, CHRISTOPHE BAUDOUIN & Pisella Pierre-Jean
`
`To cite this article: CAROLINE DEBBASCH, PATRICE RAT, JEAN-MICHEL WARNET, MAGDA DE
`SAINT JEAN, CHRISTOPHE BAUDOUIN & Pisella Pierre-Jean (2000) Evaluation of the Toxicity of
`Benzalkonium Chloride on the Ocular Surface, Journal of Toxicology: Cutaneous and Ocular
`Toxicology, 19:2-3, 105-115, DOI: 10.3109/15569520009051506
`
`To link to this article: http://dx.doi.org/10.3109/15569520009051506
`
`Published online: 27 Sep 2008.
`
`Submit your article to this journal
`
`Article views: 44
`
`View related articles
`
`Citing articles: 1 View citing articles
`
`Full Terms & Conditions of access and use can be found at
`http://www.tandfonline.com/action/journalInformation?journalCode=icot19
`
`Download by: [Emily Florio]
`
`Date: 14 September 2015, At: 06:37
`
`SENJU EXHIBIT 2064
`LUPIN v SENJU
`IPR2015-01105
`
`PAGE 1 OF 12
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`Downloadedby[EmilyFlorio]at06:3714September2015
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`103
`
`DEBBASCH ET AL.
`
`<1’
`(D
`
`(0
`EG)
`>
`LU
`
`C)
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`0
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`3
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`1:1‘
`(0
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`mean FS 355
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`M1
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`FSC-Height
`
`1023
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`mean F5 310
`
`M1
`
`Events
`
`b
`
`FSC-Height
`
`1023
`
`Figure 2. Cell size evaluation by flow cytometry after treatment with BAC. a. Control. b.
`BAC 0.001%. c. BAC 0.01%. d. BAC 0.1%.
`
`PAGE 5 OF 12
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`Downloadedby[EmilyFlorio]at06:3714September2015
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`OCULAR SURFACE BAC TOXICITY
`
`109
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`‘=3’
`(.0
`
`M1
`meanFS 260
` a
`
`Events
`
`FSC-Height
`
`B4
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`1 023
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`meanFS 61
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`M1
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`Events
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`(1
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`
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`FSC-Height
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`1023
`
`Figure 2. Continued
`
`PAGE 6 OF 12
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`Downloadedby[EmilyFlorio]at06
`
`<1‘
`(.0
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`Control
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`2% njddfi 2 2
`
`10 3
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`10“
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`‘ 10
`FL2-Height
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`BAC 0.0001%
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`64
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`Events
`Events ~»‘
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`0 .
`10°
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`,
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`.
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`.
`10‘
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`%..‘aA¢..
`.
`0
`..
`
`10‘
`10’
`10‘
`FL2-Height
`
`Figure 3. Apo 2.7 evaluation by flow cytometry after treatment with BAC. a. Control. b.
`BAC 0.001%. c. BAC 0.01%. cl. BAC 0.1%.
`
`PAGE 7 OF 12
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`
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`OCULAR SURFACE BAC TOXICITY
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`11]
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`BAC 0.001%
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`V(
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`D
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` 69% mean 49.
`
`E(D
`>
`LU
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` 0’)
`
`D10
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`10‘
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`N 0
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`10’ "
`
`10‘
`
`C
`
`$
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`FL2-Height
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`BAC 0.01%
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`
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`39% mean 49 M1 a
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`Everrts
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`Figure 3. Continued
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`PAGE 8 OF 12
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`Downloadedby[EmilyFlorio]at06
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`128
`
`6’)
`EID
`>-
`UJ
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`a
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`%
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`(O
`E(D
`>-
`LIJ
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`Downloadedby[EmilyFlorio]at06:3714September2015
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`010°
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`D
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`
`1‘
`10’
`H
`
`FL2-Height
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`Figure 4. Cellular DNA quantity evaluation after treatment with BAC. a. Control. b. BAC
`0.001%. c. BAC 0.01%. d. BAC 0.1%.
`
`PAGE 9 OF 12
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`O .
`10°
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`.
`
`10‘
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`'
`
`.
`10‘
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`FL2-Height
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`i:.
`10’
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`BAC U.[]UUl%
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`Sub-6'1
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`104
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`104
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`Downloadedby[EmilyFlorio]at06:3714September2015
`
`OCULAR SURFACE BAC TOXICITY
`
`113
`
`11'
`(.0
`
`BAC 0.001%
`
`Events
`
`
`
`in‘
`10°
`U
`FL'2-Height
`
`‘
`
`10‘
`
`BAC 0.01%
`
`°1o°
`
`10‘
`
`C
`
`%
`
`Events
`
`10°
`
`0'10‘
`
`'1
`
`10
`FL2-Height
`
`10‘
`
`d
`
`Figure 4. Continued
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`PAGE 10 OF 12
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