Curriculum vitae
`
`Louis J. DeFilippi, Ph.D.
`President,
`Louis DeFilippi LLC
`Founded July 29, 1999
`
`Independent Consulting since October 1995
`
`208 Edgewood Lane
`Palatine, Illinois
`60067
`
`Landline:
`Cell:
`
`(847)925-8524
`(847) 668-6212
`
`E-mail: defilip 1 @flash.net or Loui sDeF@Expertbiochemi st. corn
`Web: http://expertbiochemist.com/
`
`TECHNICAL ACCOMPLISHMENTS & EXPERIENCE
`Expert Consultin~
`
`EXECUTIVE SUMMARY
`
`Senior consultant, domestic and international, in the following maj or areas:
`1. Classical chemistry and biochemistry, especially the separation, purification, characterization
`and analysis of small molecules, enzymes and proteins and related processes. Adept at assessing and
`evaluating current processes and procedures!
`2. Chemical and biochemical processes, their development, evaluation, optimization, including
`biocatalysis (enzymatic and microbial) and applied biotechnology
`3. Environmental science, especially bioremediation
`4. Microbiology & fermentation science
`5. Regulatory compliance (OSHA, EPA, Groundwater, USDA, Homeland Security, etc.)
`6. Expert witness & consultant; patent infringement, claim construction, trade secrets,
`
`LABORATORY AND APPLIED BIOCHEMISTRY & CHEMISTRY: PROCESSES~ ANALYSIS
`AND SEPARATIONS SCIENCE:
`
`Broad experience and understanding of physical, chemical and biochemical processes and related analytical
`methods (assay development) and: spectroscopy, electrophoretic and chromatographic separations, protein
`modification, ligand binding, enzyme assay and kinetics, thermodynamic calculations etc.
`¯ Analytical chemistry, bioassays and separations science (especially chromatography, liquid-liquid extractions,
`countercurrent separations; electrophoresis)
`Biochemistry and biophysics combined with physical chemistry, especially through the use of UV/Vis
`(Ultraviolet / Visible) Spectrophotometry
`Separations technology for purification and analysis of numerous small molecules, proteins, peptides,
`carbohydrates, and natural products used in commercial applications
`
`¯
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`¯
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`Akermin, Inc.
`Exhibit 1013
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`Small molecule binding studies, ligands, inhibitors especially involving enzymes
`Adsorbents, ion exchange and activated carbon
`Separations science experience includes extensive application of:
`o:o Chromatographic separations
`~ DEAE cellulose
`~ CM cellulose
`~ DEAE Sephadex
`~ CM Sephadex
`~ Hydrophobic interaction chromatography (HIC)
`~ Sepharose
`~ Size exclusion
`o:o Electrophoresis
`~ PAGE
`~ SDS
`~ Cellulose acetate
`¯ :. Membrane separations
`~ Reverse Osmosis
`) Ultrafiltration
`) Microfiltration
`°.*° Liquid/liquid, Liquid/solid separations
`°.*° Precipitation using...
`) Chaotropic agents (altering solubility, salting out, precipitations)
`Salts
`Organic solvents
`
`PROCESS EVALUATION
`
`Adept at evaluating current processes at customer facilities in the Chemical, Biochemical,
`Microbiological and Food industries and suggested improvements to increase efficiency and reduce
`costs.
`
`CHEMISTRY and BIOTECHNOLOGY
`
`Expert in applied industrial chemistry, enzymology, biocatalysis, biotransformations & bioprocessing,
`especially low-waste "green" systems and thermostable enzymes; emphasis on practical aspects of research,
`development, scale-up, pilot plant and commercialization. Adept at working with both laboratory personnel
`& development engineers; hands on when needed.
`¯ Applied enz~mology: Marriage of organic chemistry and enzymology.., use of enzymes to make
`products in high yield with little waste (see examples below).
`Industrial enzyme projects include mostly "green" and thermostable industrial processes; Expert in
`continuous, heterogeneous biologically catalyzed processes (immobilized enzymes and immobilized
`whole cells).
`
`¯
`
`Preparation and application of immobilized whole cell hydantoinase suite of enzymes for industrial
`biotransformation process for production of enantiomerically-pure amino acids
`Pilot-scale immobilization of pig liver esterase for production of anti-AIDS drug and subsequent project
`management for scale-up
`Employing a statistical DOE approach for optimizing coupling of a peptide with a microbial polysaccharide for
`commercial vaccine production (Tetanus Toxoid); optimization of conditions for efficient coupling of enzyme
`to chemically-modified polymer surface
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`Akermin, Inc.
`Exhibit 1013
`Page 2
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`o Optimization of ethanol precipitation of plasma proteins for industrial plasma fractionation
`o Acetone precipitation and purification of proteins
`~ Use of salts for commercial enzyme/protein purification
`~ Development and testing of novel hydrophobic enzyme support for industrial lipase, esterase application in
`production of pharmaceuticals
`~ Fermentation, E. coli producer strain, optimization of cell yield
`Immobilization and application of genetically modified Pseudomonas sp lipase for enantioselective hydrolysis
`o
`of pharmaceutical intermediates
`Research and commercial development of novel, incompressible, high-throughput immobilized
`enzymes (food industry).
`Invention, application, and development of new method for enzyme stabilization
`~
`Invention of novel magnetic support for enzyme immobilization
`~
`~ Commercial application of thermodynamics of activation vs. deactivation (Arrhenius plot) to
`increase industrial enzyme process efficiency by 20-30%
`Other industrial bioprocessing, enzyme transformation and biochemical proj ects include:
`Purification, characterization and application of numerous enzymes, especially with metal or
`metalloid cofactors, including:
`~ Carbonic anhydrase (zinc; for CO2 sequestration)
`~ Superoxide dismutase (copper / zinc; superoxide destruction)
`~ Horseradish peroxidase (iron heme; enzyme stabilization; generation of oxidants)
`~ Catalase (iron heme; peroxide catalysis)
`~ Hemoglobin (iron heme; oxygen transfer)
`~ Glutathione peroxidase (selenium)
`o Carbohydrate conversions (see food section)
`~ Chemical modification of hydrocolloids for metal binding application (food and environmental
`applications)
`~ Hydrocolloids for enzyme immobilization (food application)
`~ Lipases for making synthetic cocoa butter
`~ Lipases (esp. Cal B, thermostable) for mono- di- and triglyceride production and
`transesterification; value added products (especially involving femlic acid, soy/soya lipids/oil, fish
`oil)
`Lactase/protease combination employed for whey treatment
`Yeast alcohol oxidase for methanol oxidation
`Glucose oxidase for production of D-glucono-6-1actone
`Glutaraldehyde as sterilant for food applications
`Biochemical fuel cell
`Microbiology, especially fermentative production of enzymes
`
`Medical and Pharmaceutical:
`o Use of applied enzymology for the production of pharmaceutical intermediates;
`o Patent holder, peptide-based anti-microbial pharmaceuticals employed in wound dressing
`applications.
`o Small molecule protection against reperfusion injury (ischemic stroke and heart attack) and
`anti-inflammatory effects of riboflavin (vitamin b2)
`o Small molecules and metals to enhance would healing rates
`
`ENVIRONMENTAL BIOTECHNOLOGY, INDUSTRIAL EFFLUENT PRETREATMENT,
`BIOREMEDIATION AND EMISSIONS TREATMENT
`
`Nationally-recognized consultant in biological and chemical treatment of air pollutants, hazardous waste,
`hazardous chemicals, water pollution, drinking/potable water
`
`Akermin, Inc.
`Exhibit 1013
`Page 3
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`Inventor of Biological Air Treater (BAT) for low-cost transfer of gases / vaporous organics (air
`pollutants) from air phase to water phase and subsequent biocatalytic transformations
`(patented/commercial)
`Microbial ecology approach to waste treatment and bioremediation
`Extensive field experience.., pilot plant direction, data analysis, scale-up estimation, commercialization;
`patent holder, biologically-based reactor development and commercialization (especially fixed-film
`reactors)
`Developed structured adsorbent used as microbial support in bioremediation of industrial effluent,
`groundwater and vaporous (air) emissions (patented/commercial)
`Developed mixed media employed as plugging-free microbial support in bioreactor packing
`(patented/commercial)
`Developed in situ-ex situ bioremediation method for detoxification of metals, especially hexavalent
`chromium, Cr(VI), by combining inorganic chemistry and microbial ecology (sulfate reducing bacterial
`consortia); patented
`Chemically modified natural polymers for use as heav~ metal adsorbers.
`On-Site international experience (China [PRC], Taiwan [ROC], UK)
`Designed and implemented numerous waste treatment and remediation strategies for diverse industrial
`effluents, impacted groundwaters, drinking water, soils, and air emissions
`Supervised replacement ofPCB (polychlorinated Biphenyl) -Transformer and replaced with non-toxic,
`biodegradable electrolyte-containing transformer
`
`FOOD AND NUTRITIONAL SUPPLEMENTS
`
`¯ Carbohydrate transforming enzymes, esp. glucoamylase, alpha amylase, glucose isomerase
`¯ Fish oil and Omega-3 fatty acids
`
`FOOD SAFETY AND FDA
`
`Food storage and handling
`Odor reduction and sanitation using chlorinated chemicals
`HACCP plans (Hazard Analysis and Critical Control Point) and process flow charts, especially for
`ready to eat foods
`SSOP (Sanitation Standard Operating Procedures); Regulatory requirements under the federal meat
`inspection act and the poultry products inspection act, especially 9cfr PART 416--SANITATION)
`
`REGULATORY, OSHA & EPA
`
`Multiple private consulting proj ects to achieve compliance with Federal & State regulations, including
`~ OSHA 1910.119, Process Safety Management (PSM)
`~ EPA 112r, Risk Management Planning (RMP);
`~ SARA Title III Emergency Planning and Community Right to Know Act (EPCRA);
`~ Storm Water Pollution Prevention Plans (SWPPP).
`Drinking water standards
`
`TECHNICAL MARKETING, SALES, MANAGERIAL & BUSINESS
`ACCOMPLISHMENTS & EXPERIENCE
`
`Akermin, Inc.
`Exhibit 1013
`Page 4
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`¯
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`¯ Experienced in all phases of commercialization business, from research to development to marketing and
`sales;
`Successfully commercialized laboratory scale reactor (biological waste treatment) through rapid
`development phase into commercial product; business profitable at 13 months; ten-fold increase in sales.
`¯ Account manager, proposal generation, expert in managing numerous $100K to $1000K projects from
`conception to commercialization.
`¯ Budgetary estimation.
`¯
`Technical sales presentations directly to customers; responsible for visiting outside customers to
`determine their actual needs and then partnering/bridging with internal experts to achieve technical and
`commercial goals.
`¯
`Technical marketing and sales, environmental area.
`¯ Excellent communication skills.
`¯
`Professional recruiting (fourteen years).
`¯ Long-term experience in TQ approach to solving cross-disciplinary problems with teams of 2 to 10
`individuals, on and off site.
`
`EXPERT WITNESS and PATENT INFRINGEMENT
`
`Experienced (defendant and plaintiff)
`Insurance investigations
`o
`o Expert witness
`~ Deposition
`~ Evidentiary hearing
`~ Preparation for trial
`~ Patent infringement, claim construction, trade secrets
`o Litigation-related experience supplied upon request
`
`EDUCATION
`
`Postdoctoral Fellow under Prof. Quentin H. Gibson, Cornell University, 7/76 - 7/78.
`Postdoctoral Fellow under Prof. Donald E. Hultquist, The University of Michigan, 1/76 - 6/76.
`Ph.D., Biological Chemistry, The University of Michigan, 9/71 - 1/76.
`BA, Chemistry (Honors), Queens College, The City University of New York 5/71.
`
`SELECT TRAINING/DEVELOPMENT/PRESENTATIONS
`
`HAZWOPPER Emergency response Refresher Training (40 hour initial training, 8 hour Haz. Waste
`Operations Management continuing yearly), 1991 to Current (1995).
`Emergency Notification Release Reporting Training, EPCRA 304; CERCLA 103 (EPA Region 5),
`1999
`Risk Management Program Assistance (IEMA), 1999
`Basic Ammonia Refrigeration, 1998
`Statistical Design of Experiments (DOE), 1996
`Scorecard Training, 1996
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`Akermin, Inc.
`Exhibit 1013
`Page 5
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`Convenor, Pollution Prevention Biotechnology, Summer Biotechnology Institute, Northwestern
`University Center for Biotechnology, 1995
`Sales Training Workshop (Institute for Business and Interpersonal Strategies) 1995
`Customer Linked Commercialization & Customer Partnering, 1994
`
`SELECT PRESENTATIONS
`
`"Treatment of Wastewater for Re-Use Applications Using an Immobilized Cell Bioreactor System", 2013
`Industrial Water Reuse Specialty Conference, Long Beach, CA, 9-10 December 2013
`
`"Physical chemistry and separations principles for [bio-catalyzed (enzymatic) process] yield
`improvement", 9th International Symposium on Biocatalysis and Biotransformation, Berne, Switzerland,
`5-9 July 2009,
`
`"How efficient is my enzymatic process? A little help choosing the right standard curve using error
`analysis", Gordon Conference, July 2008, Rhode Island
`
`"Thoughts on International Trends (China) and Emerging Water Issues" Invited speaker, Environmental
`Practice Specialty, American Society of Safety Engineers ASSE Headquarters, 1800 Oakton, Des Plaines,
`IL, USA. April 13, 2007
`
`"Chemical modification, physical chemistry, biotransformation and separations science principals
`combine for the improvement of immobilized enzyme biocatalytic productivity", University of Iowa 14th
`Annual Center for Biocatalysis and Bioprocessing Conference on Pharmaceutical and Industrial
`Biotechnology, October 2004, Iowa City, Iowa
`
`"Use of chemical modification, and application of physical chemistry and separations science principals,
`for the improvement of immobilized enzyme biocatalytic productivity", Gordon Conference, July 2004,
`Vermont
`
`EMPLOYMENT HISTORY
`
`Industrial
`
`¯
`Independent Consultant, President and Member, Louis DeFilippi, LLC, 1999 to present
`¯
`Independent Consultant, Sole proprietorship, 1995 to 1996 and 1997 to 1999
`¯ Project Leader, AlliedSignal Aerospace Sector, Extended Consultation Assignment (Space Station /
`Mars Lander / Lunar Base), Life Support Systems, ’96 to ’97
`¯ Manager Biochemical Technology (Technical Marketing and Sales), AlliedSignal Environmental
`Systems and Services, ’94-’95
`¯ Research Scientist, AlliedSignal Research & Technology, ’85-’94
`¯ Research Specialist, Biochemistry, Signal-UOP Research Center, ’82-’85
`¯ Senior Research Biochemist, Biotechnology Department, UOP Research Center, ’78-’82
`
`Academic
`
`Akermin, Inc.
`Exhibit 1013
`Page 6
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`Adjunct Professor (Evening Faculty Member), Columbia College, Dept. of Science, ’84-’ 85.
`Postdoctoral and Teaching Fellow (General biochemistry), Cornell University, Department of
`Biochemistry, Molecular and Cell Biology, ’77-’78.
`Teaching Assistant (General biochemistry, Medical Technology & Nursing) The University of
`Michigan, ’71-’ 75.
`
`HONORS
`
`Special (Team)Recognition Award, AlliedSignal (Honeywell), April ’95
`Special (Individual) Recognition Award, AlliedSignal (Honeywell), May ’92
`Numerous inventor awards
`Rackham Dissertation Grant, The University of Michigan, ’74-’75
`Graduated (BA, Queens College) with honors, ’71
`Elected to Beta Delta Chi, Chemistry_ Honor Socie _ty, Queens College, CUNY, ’71
`New York State Regents Scholarship, ’67-’71
`
`SOCIETY MEMBERSHIPS
`
`American Chemical Society
`Water Environment Federation
`
`PATENTS AND PUBLICATIONS
`
`Authored approximately 22 publications, 3 book chapters, and is an inventor on approximately 15 issued
`patents, in biotechnology, chemical, food, pharmaceutical industries, and air and wastewater biological
`treatment areas; co-Editor, John Wiley & Sons, "Biological Treatment of Hazardous Wastes" 1998.
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`Akermin, Inc.
`Exhibit 1013
`Page 7
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