EXHIBIT 1054
`
`Article entitled “Smart Camera” in the Microscopy and
`
`Analysis publication dated July 1993
`
`TRW Automotive U.S. LLC: EXHIBIT 1054
`PETITION FOR INTER PARTES REVIEW
`OF U.S. PATENT NUMBER 8,599,001
`IPR2015-00436
`
`

`
`MICROSCOPY & ANALYSIS
`ISSN 09581952
`PUBLISHER
`Jean R. Gordon
`TECHNICAL EDITOR
`Ian M. Watt
`ART EDITOR
`Tony Lees
`LAYOUT ARTIST
`Barbara Parker
`CIRCULATION MANAGER
`Lynda Stowell
`CIRCULATION ASSISTANT
`Astrid Baillie
`PRODUCTION MANAGER
`Agnes Murphy
`MARKETING CONSULTANT
`Peter D. Gordon
`ADVISORY PANEL
`J. E. Beesley, Wellcome Research Labs,
`Kent
`R. A. S. Blackie, J. S. Pathology, London
`B. Bracegirdle, Science Museum, London
`S. Bradbury, Pembroke College, Oxford
`Gillian Bullock, Ciba-Geigy Limited,
`Switzerland
`C. Cook, Consultant, Westbury
`D. J. Dyson, British Steel plc, Rotherham
`P. J. Goodhew, University of Liverpool
`P. W. Hawkes, CNRS, Toulouse, France
`C. J. Humphreys, University of Cambridge
`N. J. Long, University of Oxford
`M. H. Lorette, University of Birmingham
`G. Love, University of Bath
`Katherine Mclaren, University of
`Edinburgh
`T. Mulvey, University of Aston
`M. V. Nermut, Nat. Inst. Biol. Standards
`& Control, I.ondon
`A. W. Robards, University of York
`J. A. Swift, Unilever Res Lab, Merseyside
`J. Venables, University of Sussex &
`Arizona State University
`PUBLISHED BY:
`Rolston Gordon Communications
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`
`
`COVER STORY
`The cover shows a 3-D confocal reconstruction of a human
`placenta syncytiotrophoblast. Grown five days in culture, the
`cell was stained with a fluoresoeinated antibody to trophoblast
`membrane’-* (green) and counterstained with propidium iodide
`to label the multiple nuclei (red).
`The confocal fluorescence slices were collected using a
`Meridian Instruments INSIGHT PLUS Laser Scanning
`Confocal Microscope System with a 100x oil
`immersion
`objective (NA 1.4), an argon ion laser, the INSIGHT PLUS
`Multi-Color Argon Laser Filter Wheel with 605 longpass and
`530/30 bandpass filters, and the Meridian Cooled CCD. The
`3-D reconstruction was generated from the slices using the
`premium INSIGHT-IQ Computer System from Meridian
`Instruments. (Courtesy ofDon Thompson, Dr. In C. Kim, and
`Dr. Gloria Sarto, Department of Obstetrics and Gynecology,
`University of New Mexico, Albuquerque, New Mexico.)
`The INSIGHT PLUS (shown above) is a modular system that
`offers through-the-oculars confocal viewing at 120 scans per
`second. It is available on upright or inverted fluorescence
`microscopes from several manufacturers, including Olympus
`Corp., Nikon Inc., and Carl Zeiss Inc. The INSIGHT PLUS
`system can be used either in confocal mode (fluorescence or
`reflectance) or in nonconfocal mode (transmission), and is able
`to acquire and store as many as 30 confocal images per second.
`INSIGHT PLUS system options include: air-cooled argon
`laser systems; an air-cooled krypton/argon laser system; filter
`wheels; reflectance optics; premium computer hardware and
`image acquisition and data analysis software; a cooled CCD;
`35mm camera and automatic photomicrographic system; Hi8
`video deck; video printers; a micro-injector; and an incubator.
`Meridian Instruments Europe, Inc., Industriepark-West 75,
`B-9100 St. Niklaas, Belgium; Tel: (32) 3-7801760.
`* In C Kim, J.Hial Chem (1989), 264: 9780-9784
`
`CIRCLE READER ENQUIRY NO. 1
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`FREE SUBSCRIPTIONS
`The date in the top right hand corner of your address
`label is your expiry date. Ifthis date is July 1993
`or earlier you need to complete and return a new
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`back of this issue) to continue your free subscription.
`
`MOON ROCKS UNDER THE
`MICROSCOPE
`Michael W. Davidson
`
`Page 5
`
`POTENTIAL SKIN SUBSTITUTES:
`A CLSM STUDY
`M. Hanthamrongwit, R. Wilkinson,
`M. H. Grant
`
`Page 9
`
`APPLICATION OF MONTE CARLO
`SIMULATIONS IN SEM
`Eli Napchan
`
`Page 11
`APPLYING ERGONOMICS TO
`IMPROVE MICROSCOPY WORK
`Helen Haines and Lynn McAtamney
`
`Page 15
`
`BIOLOGICAL SCANNING
`TRANSMISSION ELECTRON
`MICROSCOPY
`S. Brian Andrews and Richard D. Leapman
`
`Page 19
`
`MICROWAVES AND
`TISSUE PROCESSING
`David Hopwood
`
`Page 25
`
`3-D RECONSTRUCTION AND
`LATERAL VIEWS OF BIOLOGICAL
`SPECIMENS
`Alberto Diaspro
`
`Page 29
`
`QUANTITATIVE ULTRASTRUCTURAL
`IMMUNOCYTOCHEMISTRY
`Sverker Enestrtim
`
`Page 33
`
`CRYSTALLOGRAPHIC IMAGE
`PROCESSING ON A PC
`Sven Hovmiiller
`
`Page 37
`
`COMPANY NEWS
`
`Page 39
`
`EVENTS DIARY
`
`Pages 43 and 45
`
`BOOK REVIEW
`Page 47
`
`HIGHLIGHTS OF CURRENT
`LITERATURE
`
`Pages 48 and 50
`
`PRODUCT NEWS
`
`Page 53
`
`1054-001
`
`MICROSCOPY AND ANALYSIS, JULY 1993 3
`
`1054-001
`
`

`
`Recently introduced in the 1993 Vector
`Laboratories catalogue, DAB enhancing
`solution is a new reagent useful for toning
`DAB stained sections.
`Designed as a simple, one-step addition
`to the staining sequence, enhancement
`takes from 5-20 seconds and generally
`tones the DAB reaction product
`to a
`darker, reddish-brown colour. This treat-
`ment also prevents any decolourisation
`during dehydration and permanent
`mounting.
`Offered as a 30ml package, the DAB
`enhancing solution can tone approxi-
`mately 300-600 slides depending on the
`volume employed per slide. It
`is not
`designed for use with other peroxidase
`substrates, such as AEC or Vector VIP
`(Purple), except that it does somewhat
`enhance the new Vector SG (blue-grey)
`precipitable substrate for horseradish
`peroxidase.
`a new
`For more information or
`catalogue, contact: Vector Laboratories
`Ltd., 16 Wulfric Square, Bretton, Peter-
`borough PE3 8RF, UK. Tel: 0733 265530.
`Reader Enquiry No. 109
`
` Please use your address
`label carrier for the fastest
`
`way to respond to items
`mentioned in this issue
`
`FOR IMAGE ARCHIVING
`Aequitas IDA Image Database and Image Archive Management software uses an Entry
`Level Vision System, ELViS for short.
`This displays live video on the VGA screen of a PC. It accepts composite video signals,
`converts them to a digital format which is processed then displayed.
`The process can be stopped at any time, freezing an image to allow software to access
`it and if required compress it, add graphics and save it with associated text and data.
`ELViS H is compatible with 1024x728 pixel VGA systems and features broadcast
`standard colour encoding (4:2:2).
`Details from Dynamic Data Links Ltd, PO Box 31, Elsworth, Cambridge CB3 8LG.
`UK. Tel: 095 47 309/0954 789703.
`Reader Enquiry No. 110
`Slow scanning CCD TV systems permit Transmission Electron Microscopes to be used
`without the need for a dark room. A new Hitachi Technical Data Sheet (number 65)
`features the use of SSCCD TV on the H-9000NAR TEM. Copies are available free from
`Hitachi Scientific Instruments, Hogwood Industrial Estate, Finchampstead, Wokingham,
`
`Berkshire, RG11 4QQ, UK. Tel: 0734 328632.
`Reader Enquiry No. 158
`
`
`
`the first miniature imaging
`imputer,
`computer that can analyse what it sees.
`This tiny ‘smart’ camera, measuring only
`107X53><24mm, can be programmed to
`perform a wide range of functions, from
`industrial inspection tasks to multi-media
`uses. The arrival of the imputer (imaging
`computer) means that vision product
`development has become accessible to all
`product developers,
`expensive, highly specialised and
`available to only a few companies.
`Programming is very straightforward
`using the Windows-3.1 compatible IDS
`software developed by VVL for
`purpose. These two factors make it
`attractive and profitable for companies to
`develop and market programmed
`imputers as smart vision modules.
`The imputer is not tied to any particular
`vision application, but is a completely
`generic development platform for vision
`products. The unlimited range of
`applications includes inspection, process
`control, smart surveillance,
`monitoring and control, numberplate
`recognition, multi-media, character
`recognition,‘ biometrics, metrology, fluid-
`flow dynamics, automotive, navigation
`aids, robotics and vision toys. Details from
`VLSI Vision Ltd, Aviation House, 31
`Pinkhill, Edinburgh EH12 8BD. Tel: 031
`539 7111. Reader Enquiry No. 111
`
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`K. E. DEVELOPMENTS LIMITED
`The Mount, Toft, Cambridge. CB3 7RL.
`Yellphona 022: 263532 Flculmlla 022: zszsaa
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`Data Sheet with micrographs directly
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`imported from Printerface Plus.
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`
`The Mount, Toft, Cambridge, CB3 7RL, England.
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`filnnr N0. 29
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`1054-002
`
`MICROSCOPY AND ANALYSIS, JULY 1993 59
`
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