Attorney Docket No.: NEPN-001/OIUS (313663-2001)
`
`IN THE UNITED STATES PATENT AND TRADEMARK OFFICE
`
`Applicant:
`
`Fotini Sampalis
`
`'
`
`Confirmation No.:
`
`3094
`
`Application No.:
`
`10/485,094
`
`Filed:
`
`July 13, 2004
`
`Art Unit:
`
`Examineri
`
`1614
`
`Gregg Polansky
`
`Title:
`
`'
`
`NATURAL MARINE SOURCE PHOSPHOLIPIDS COMPRISING
`
`FLAVONOIDS, POLYUNSATURATED FATTY ACIDS AND
`THEIR APPLICATIONS
`
`Mail Stop Declaration
`Commissioner for Patents
`
`P.O. Box 1450
`
`Alexandria, VA 223 1 3-1450
`
`DECLARATION OF EARL L. WHITE PH.D. UNDER 37 C.F.R.
`1.132
`
`
`
`1, Earl L. White, declare as follows:
`
`1.
`
`2.
`
`I am a U.S. citizen.
`
`I am presently President and CEO of MDX BioAnalytical Laboratory, Inc. in College
`
`Station TX, which offers, among other things, expert analytical services for identification of
`
`biosubstances.
`
`3.
`
`I have a Ph.D. in analytical chemistry and mass spectrometry from University of North
`
`Carolina at Chapel Hill. Since 1985 I have worked professionally in the area of analytical
`
`chemistry and mass spectrometry (26 years). I have served as Adjunct Professor of Chemistry,
`
`Wake Forest University, Dept. of Chemistry and have published 27 peer reviewed journal
`
`articles.
`
`I presently serve as a Peer Reviewer for J Chromatographic. Science and J.
`
`Chromatography.
`
`I am a member of the American Chemical Society (CNCS—Section Chairman
`
`'93, Chair-Elect/Program Chair '92, Secretary '91 and Treasurer '90), Mississippi Academy of
`
`Science, Society of Analytical Chemists of Pittsburgh, Applied Spectroscopy Society, Phi
`
`Lambda Upsilon Honorary Chemical Society, America Society for Mass Spectrometry, National
`
`Technical Association and Alpha Phi Alpha Fraternity, Inc., Triangle Area Mass Spectrometry
`Petition for Inter Panes Review
`Of U.S. Patent 8,278,351
`Exhibit
`
`_
`
`ENZYMOTEC - 1051
`
`000001
`
`/
`
`-1...
`
`000001
`
`

`
`Attorney Docket No.2 NEPN-001/0lUS (313663-2001)
`Declaration of Earl L. White, Ph.D.
`
`Discussion Group, National Organization of Black Chemists and Chemical Engineers
`
`(NOBChCE), Bay Area Mass Spectrometry Discussion group (BAMS) and the SF Bay Area
`
`Proteome Society. My CV is attached and labeled as Attachment A.
`
`4.
`
`I am considered an expert in the area of identification of biomolecules using analytical
`
`techiques in general, and mass spectrometry, specifically.
`
`5.
`
`MDX BioAnalytical Laboratory, Inc. was contracted by Neptune Technologies and
`
`Bioressources of Québec, Canada to analyze certain phospholipid samples (the “Beaudoin Oil
`
`Fractions”) and report which fatty acid phospholipids are present in the Beaudoin Oil Fractions.
`
`Furthermore, I was asked to opine on whether certain fatty acid phospholipids were present or
`
`absent in the Beaudoin Oil Fractions. The result of this analysis follows.
`
`6.
`
`I received three Sample Sets from Neptune Technologies and Bioressources, each of the
`
`first two Sample Sets containing three separate Fractions labeled Beaudoin Oil Fractions I, II and
`
`III, and the third Sample Set containing two separate Fractions labeled Beaudoin Oil Fractions I
`
`and III, for liquid chromotography/mass spectrometry (“LC/MS”) and liquid chromotography—
`
`mass spectrometry/ mass spectrometry (“LC—MS/MS”) analysis. LC/MS is an widely accepted
`
`separation and identification technique used in the pharmaceutical industry to characterize
`
`organic molecules.
`
`I was to analyze the eight Fractions in the three Sample Sets and identify the
`
`fatty acids attached to the phospholipids (PL) present in each of the Fractions in each Sample
`
`Set. The PLs of interest include phosphatidylcholine (PC), phosphophatidyl—ethanolamine (PE)
`
`and phosphatidylinositol (PI).
`
`METHODS
`
`7.
`
`All Fractions received were analyzed by LC/MS. Each Fraction was dissolved in
`
`chloroform and diluted to a concentration of 500 ng/uL in 20% MeOH in 0.1% formic acid (FA)
`
`and subjected to LC/MS. A 1 or 2 uL aliquot of each sample was injected onto a C18 column,
`
`separated on a Waters MicroMass CapLC and detected with a Waters MicroMass Q—TOF Micro
`
`quadrupole time—of—flight mass spectrometer with nano—electrospray ionization (ESI). The
`
`instrument was operated in the full scan or MS/MS positive ion mode for these Fractions.
`
`1 When the third run was performed for the third Sample Set there was not enough material remaining after the ethyl
`acetate Fraction for analysis.
`
`-2-
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`
`Attorney Docket No.: NEPN-001/01US (313663-2001)
`Declaration of Earl L. White, Ph.D.
`
`Continuum mass spectra were acquired over the mass range of 400-2000 amu with a scan time of
`
`1.80 s and an inter-scan delay of 0.1 s. The capillary, sample cone and extraction cone voltage
`were 3672, 30 and 2.5 V, respectively. The source temperature was held at 120°C, the cone N2 I
`
`gas flow was 50 L/h and the collision energy was held constant at 10 V. The HPLC column was
`
`a CVC Micro-Tech C18WS-150EU nano column. HPLC/MS procedure; mobile phase A (0.1%
`
`FA H2O:ACN, 95 :5%) was held at 20% B (ACN: 0.1% FA in H20, 95 :5%) for 4 min. and then
`
`programmed to 70% B at 5 min., 80% at 15 I’I1l1‘1.\tl1CIl to 100% B at 30 min. and held for 36 min.
`
`The flow rate was 7 uL/min. with a split ratio of about 6:1 yielding a flow through the column of
`
`approximately 600 nL/min. For MS/MS analysis, the Q-TOF was operated in the survey scan
`
`mode where data dependent MS/MS analysis was performed on all ions detected above a pre-
`
`determined intensity threshold. Optimum collisionenergies for compound dissociation were
`
`determined. Each Fraction was analyzed in duplicate for MS scan and at least once in the
`
`MS/MS mode.
`
`8.
`
`My full report is attached to this Declaration as Attachment B.
`
`RESULTS
`
`9.
`
`A list of fatty acid phospholipids detected in the Beaudoin Oil Fractions is given in the
`
`Tables 1 and 2 (see Attachment B) with relative percentages based on area under the curve with
`
`the caveat that all PLs do not necessarily respond the same. These values should therefore be
`
`considered a semi-quantitative representation of the Various PLs.
`
`10.
`
`Table 1 presents the results of Fractions 1, II and III for each Sample set. Table 2
`
`presents the same results grouped by Sample‘Sets 1, 2, and 3 for each Fraction therein.
`
`PLS detected in the Fractions were primarily phosphatidylcholines as confirmed by the
`1 1.
`characteristic m/z 184 ion in the MS/MS spectra. With the exception of PL with molecular
`
`weight (MW) of 732.5 Da, PLs in an amount of 3 5% were low MW PLs. Six of the PLs
`
`detected in the samples (m/z 542, 568, 666, 808, 826 and 852) have molecular weights, which,
`
`without further analysis, could be considered evidence of a PC containing DHA and EPA, or a
`
`combination of DHA and EPA, however MS/MS spectra shown in the figures below confirm
`
`that these PLS do not have attached to the sn-1 and sn-2 positions of the glycerol backbone DHA
`
`and DHA, EPA and EPA, DHA and EPA, or EPA and DHA.
`
`000003
`
`-3-
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`

`
`Attorney Docket No.: NEPN-001/01US (3 13663-2001)
`Declaration of Earl L. White, Ph.D.
`
`Figures 1 — 3 show representative total ion chromatograms for 500 ng/uL of each oil
`12.
`sample dissolved in CHCI3. Peak areas were calculated from the protonated molecular ions for
`each of the major peaks in the spectra. MS/MS spectra for a representative Beaudoin Oil
`
`Fraction sample are shown in Figures 4-9, and 11. Figure 10 is a representative MS/MS
`
`spectrum for a PC with a molecular ion at m/z 826.5. Fragment ions detected at m/z 524.3 and
`
`542.3 are indicative of 302 and 284 Da losses from the molecular ion which is indicative of the
`
`loss of a C20:5 (EPA) and C1820 fatty acids from the protonated molecular ions. However, there
`
`was no ion observed at m/z of 499 which would have been indicative of a loss of 327 which is
`
`DHA from the protonated molecular ion at m/z of 826, which is indicative that there is no DHA
`
`attached to the phospholipid.
`
`13.
`
`It is my opinion that the Beaudoin Oil Fractions received and tested by me do not contain
`
`‘ PLs which have attached to them DHA and DHA, EPA and EPA, DHA and EPA, or EPA and
`
`DHA, at the detection levels described above in paragraph 7 or represented in the spectra of
`
`Figures 1-1 1.
`
`[rest of page intentionally left blank]
`
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`

`
`Attorney Docket No; NBPN-001/OIUS (3l3663~2001)
`Declaration of Earl L. White, Ph.D. .
`
`14.
`
`I further declare that all statements made herein of my own knowledge are true, and that
`
`all statements made on information and belief are believed to be true, and further, that these
`
`statements were made with the knowledge that willful false statements and the like so made are
`punishable by fine or imprisonment, or both, under Section 1001 of Title 18 of the United States
`
`Code, and that such willful false statements may jeopardize the validity of the application or any
`
`patent issuing thereon.
`
`
`
`Earl ‘L. White, mo.
`
`Dated: May 31, 2011
`
`77227 vl/BN
`
`000005
`
`000005
`
`

`
`ATTACHMENT A
`
`UEIARL L WHETE - Currisruium Vitae
`
`]‘v’iiI)x Bie/tiiaiytieai Laberatery, Erie.
`R0. Be); 236
`
`Weiibein, TX 77881
`
`Oftiee: (979) Sir-$7368
`Ceii:
`€520‘; 9816296
`
`
`
`
`Eiiiieatieiiz
`
`University 0fN0rti1 Carolina
`at Chapel Hiii
`Chapel Hill, North Carolina
`iviajer: Chemistry (/xnaiytieai)
`Gra,dua.ted: June i987
`
`University ef Pittsburgh
`iPisttsburgh, Penrisyivzirii a,
`M aj oi‘: Ci'3einist1”y (Anaiyticaii)
`
`Degree: P118.
`
`1983-1987
`
`Spectrometry
`
`1975 ~ 1 977
`
`1975
`
`1973 ~ 1 974 (p§.’:1’E‘,-iilllfa)
`
`Degree: MS.
`
`Jeieksen State University
`;iaei<s0ri, Mississippi
`iviajer: Ciiernistry (Organie~Ari-aiytieai)
`Gradua.ted: August i975
`University sf Mississippi Medical
`Center, Jaei<s<_in, Mississisppis
`
`Degree: B&
`
`3967-1971
`
`Unrlergrarluate Seheei:
`Jackson State Ceiiege
`;iaei<s0ri, Mississippi
`iviajer: Chernistry
`G1‘a,dua.ted: December ii 971
`
`Prefessienaii Experieiiee:
`
`
`
`
`
`
`
`ii/G8—preserit President :3; CEG
`Established MD); in 2008 and ineerporated in 2009, inanage business component and
`directs tf3Ciii”,‘tiCa,i furietiens. Maiiages bu: get, prepares quotes, proposals, pinteeeis and
`reports for services peifermeci.
`
`
`
`Vice President, Anaiytieel & Fiimmaeeutieai Sciences
`G7/G7~ 11/GS
`Manage Advance Anaiytieai and Quality Ceritrei ,t’uneti<_ins at the €er'pera,te
`senior managemerit with the staff estimates and budgetary" i‘equiremerits.
`
`ievei. Assist
`
`000006
`
`000006
`
`

`
`(CV Coritkl)
`
`l?.ARll lg. ‘Wlll'"l”l?.
`
`
`{)9/02- 04,/l-,/2‘;ll{l7 Vice l’resldent,, Advanced Analytical Sciences & lfirector, Quality
`Control
`
`to
`lunctiorls at the corporate level
`lvlanage Arlvance Analytical and Quality Control
`include QC operations in Owerisboro, KY and Gerniantown, Ml). Assist senior
`management with the staff estimates and budgetary requirements,
`esearch and
`development focuses.
`
`l“}lreetor, Advance Analytical Resources & Quality Control
`ll)/99439/’(l2
`Established and directed the Advanced Analytical Resources Division including Assay
`Development Group. Directed Quality Control Group for production of pharmaceutical
`products under eGlvlP guidelines (proteins expressed in ]\7icotz‘a:zna bcztzzizazmrzna plants)
`including Phase l Clinical
`‘trials of at non—l*lodgl<in’s lyrnplioma vaccine. Releztsc
`vaccines to Quality Assurance for patients use and maintained stability studies. Directed
`proteomics lunctions Within the Advance Analytical Resources Division.
`
`Large Scale Blolofi 7 Cor oration lormerl Blosource Technolo ties lne. . Vacavllle CA
`
`l,€l,"98~lG/99
`
`Sr. Scientist
`
`Developed Advance Analytical Resources Group which included stalling with
`approximately l0 scientist and purchasing of mass spectrometric equipment to include
`GC/'l\/lS, l‘vlALDl—TOF, LC/Q—TOF i‘vlS/lvlS and LCQwl\/lS/'l\/l-S.
`
`R3. Revnolds Toliaeco Corn an ’ W/lllsttin-§§3l£?EFl NC
`
`
`
`
`
`Sr. Staff l~t&l) Clieniist, M ass Spectrornetry Group Leader
`l2/'88~(l9./98
`Technicatl responsibilities include; analytical methods
`development and analyses,
`operation and maintenance of gas clnoinatograplt/mass spectrometer/data systems, report
`writing and technical puhlicatiort/presentations.
`Supervisory and managerial responsibilities included; leatlership of mass specti‘onietr_v
`group, project management, budget proposal, capital equipment purchase, supervision of
`personnel, personnel evaluation and directed postdoctoral fellow research, etc.
`
`l2/85—l 2/88
`
`Rtlltll t.Ilietrilst
`
`Responsibilities include; analytical methods development and analyses, operation and
`maintenance of gas cln‘oinatograpl1/mass spectrometer/data systems, report writing and
`technical publication/presentations.
`
`lslatlonal lnstltutc ofllinvlrornnental llealtli Sciences NEEHS‘ Research "lrlaii
`
`Clieniist/Graduate Student Q-/tppointtnent
`(39/’S3—l2,"85
`Graduate Student, Teaching Assistant, Research Assistant, Chemist on "P Appointment"
`with NlEHS performing ion—inolecule reaction studies using triple quadrupole mass
`spectrometry (M S/MS}.
`
`le Perl»; NC
`
`
` ............................... ..
`
`......................... ................ .
`l98 l—l983
`Associate Scientist, Head, Analytical Services L-nit,
`Performed duties consistent with those of a department head to include budget and
`project managernent, proposal writing, equipment purchasing, writing reports, and
`2
`
`000007
`
`000007
`
`

`
`lEARii lg. Vi/liili”l?.
`(CV Coiitkl)
`clironiatograph/'rnass
`operation
`and maintenance of gas
`supervision,
`personnel
`spectronieter/'d.alta system, pl1?c11"l11§i(.‘.0l{iI1€§l.lf.?S researcll, scientific writing presentations and
`publications.
`
`19774 981
`
`Senior Research Associate
`
`Performed toxicological research on environmental chemicals in small animal studies.
`Also, published the first peer—reyiewed manuscript for CHT.
`
`‘Westin iioiise R&l1= Pittsiitirvii PA
`
`
`
`
`
`Silrnnier— l 977 Analytical Clienii:-it
`Sumrner eniploynient during gratlnate school.
`
`University of Pittsliiirgii. Pi‘iZiSlitll‘gii: PA.
`()9/75—l 2/77
`Teaciiiiig/Research Assistant and Gratlaate Student
`'l'ai.ight uritlergraduate cliernistry laboratory and performed r‘esearch.
`
`Mississi
`
`re Crime Laliorator’/llniyersity of Mississi :2 Medical Center. .lael<;son li/15$
`
`instrumentation Specialist/Section Cliief, Toxicology’
`12/7:‘-i~8/'75
`Same as below with 2t.(‘lClltl0l,l£tl,
`l,'f3S}30TlSll’)l,lliy of rnass spectrornete (lVlS) purchase and
`Supervision of M S operator‘.
`
`{ll/73—l2/'74 Toxicoiogist i/Election Chief, Toxieiilogy Section
`As Section Clliel’, Toxicology Section, coorclinated personnel, analysis of suspectecl drug
`overtlosage sarnqilles, operation and rnaintenance of
`elironiatographs/cliron’iatographic
`data
`system,
`eVali.iation,
`specification writing,
`and
`purchasing
`of
`scientific
`instrumentation and perforrnetl hlootl alcohol analyses.
`
`G7/’72«—0l/73 Toxieologist l
`Perl,”ormecl analysis of suspected cling overiiosage biological sarnplesl operation and
`maintenance of
`chroniatograplis.
`
`Arniy Medical Hospital, llort tlaelésting South Carolina
`G2/72«—06./'72 Lalioratory Technician
`
`lliiiversity of Mississippi Meiiieal Center, Jaitkson, MS
`€36/'7l)~(l2./7
`Laboratory Teeliiiician
`
`Appointments:
`Atliurict Professor of Cliemistry, Wake Forest Unilversity, Dept. of Chernlistry
`Liaison, R.lR_—Corpoi'ation Associate, American Cliernical Society
`Co—AdVisor, graduate student program with North Carolina A851‘ State University
`Visiting Professor, National Urbain League Black Executives Exchange Program (BEEP)
`Adjunct Visiting Professor, SciWorl:<s of VVi,t’lSi01l~SEii6lTl
`
`Additional :i3:Xp€l'it3l'tCi3SZ
`Proficient in the o aeration and maintenance of these Analytical instruments:
`3
`
`000008
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`

`
`EARL L. Vi/Hl'"l”E
`{CV Coiitkl)
`Gas chroniatograph/mass spectronieter/tlata system, ultra—Violet Visi'ble—, infrarecl—, atomic
`abscrption/'eniission
`spectropliotometers,
`pH meter,
`spectrotluorometers,
`high
`perlcr'ii'iai1ce liquid Cill”0lT1Ell.OgI‘2t.pll,S, i3MlT ainailyzer systein, liquicl S€.‘.lt’llilll,2i.l,i0Il counters,
`electrospray/Al’Ci—MS, capillary electroplioi‘esis»MS aiitl l\/iS/"IVES.
`
`Gttiers:
`
`Peer Reviewer:
`
`lourrial — J. Chromcztograpizic. Scz"enr:e=
`Journal — J. Cizr()maiogmpi*zy
`Site Visitor/Gi‘ant proposal reviei rer---NSF Grant l’roposal—lncarnate Worcl College (1994)
`
`Military:
`
`Lieutenant Colonel — Retired ifApril 30, 2009 W,/34.66 years of service)
`
`United States Arrny National Guard, December l 97 l — l 977
`ll2lh MP Battalion, lei ill), lacksonc, MS ~ Clerk Typist — 4 Years
`
`ll)’/"lb Field Artillery, HHC, llittsbnrgli, l’A, Battery Aitlnian ~ 2 years
`Discharge: Honorable
`
`United States Ariny Reserve, August l9Sil to September i988
`80:3”; Nil’ Company (CS), Nuclear‘ Biological
`Clieiiiical (l\lBC) l\‘=CO — November i984
`Direct Coniniission as Cheniical Officer, Noveniher l 984
`
`Tours ofDii ty (three weeks, each)
`Suhic Bay, Philippines (l987)
`Suhic Bay, Philippiiies (3988)
`United States Arniy Reserve, September i988 to present
`Direct Connnission, Chemical Corp, i984 (ZLT)
`Direct Comrrriissiron as Merlical, Service Oliticer, Septernber i988, Current Rarnlt (lls/lager‘)
`l-"lreyioiuslyz llVlA,
`llteteiise intelligence Agency (DEA), Washington, D.C., Al*‘l‘vllC, Ft.
`Detrick, Fredrick, MD, William Beaumont Army Medical Center, Ft. Bliss, TX
`Sr. Observer/Trainer, 9lSl Division, lsl Btlfi, C-SS—Tasl{ Force, Camp Parks, Dublin, CA
`CU.l’l'€31'lllyZ
`LTC, IVES; Enviroriniental, Science Ollicer/liistriictor — 2ND Medical Brigade./'
`
`WMD l-"liograin
`
`Membership:
`Ainerican Cheinical Society (CNCS~Section t'_3hair'ii'ian ‘93, Cliair~ Elect/’Prograrn t'_3lia.ir '92,
`Secretary ’9l and Treasurer '90), Mississippi Acadeniy of Science, Society of Analytical
`Chemists of Pittsburgli, Applied Spectroscopy Society, Phi Lambda Upsilon Honorary
`Cheniical Society, Aineriea Society for Mass Spectrometry, National Technical Association
`ancl Alpha Phi Alpha Fraternity, lnc., Triangle Area Mass Spectrometry Discussion Group,
`National {)rgani;:ation of Black Chemists and Chemical Engineers (l\l{)BChCE), Bay Area
`Mass Spectronietry Discussion group (BAMS) and the SF Bay Area l’roteonie Society.
`
`Civic Activities:
`
`Visiting Pi'ofessoi', Natioiial Uihaii l..eag1.ie’s Black Execiitiyes Exchange Prograin (fiElElEil’),
`(l986—l 9979).
`
`SciWorl<:s of Winston~Saleni ature Science Center), Adjunct Professor, (1993-1998).
`
`000009
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`000009
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`

`
`{CV Coritkl)
`Co-coorclinator, "l‘utoria.1 Prograrn, Unitect 1\/letr‘opolitan Missionary Baptist Church, (1992-
`i998).
`
`EARL 1;. ‘WHl‘"l"E
`
`lvterliatiori Services of Forsyth County, Meinber
`Boa.rtl~of-Directors,
`President — (1994), Presitleiit and Chairman of the Boarrl (19954 998).
`Advisory Board Council, Forsyth Center for the Deaf and Hard of Hearing (1993-1998)
`Paniicipant/Denionstrator, Local clenientary, iniddle, high schools and colleges.
`
`—
`
`(1 993), Vice
`
`Certificates and Awa.ro‘.s:
`
`interpretation of lrifrarctl Spectra, Mississippi Section of The American Chemical Society,
`ACS Filrn Short Course, January 22,1971.
`
`Retention Data in Gas Clironiatography, Wasliington University School of Continuing
`Erlucation, October lo, 1973.
`
`CNTA Meritorious Service Award, 1982.
`E1ectecl Who’s—Who in the South and Southwest in 1983.
`
`Recipient of the [NSF Fellovvsliip, 1984~8:”>
`
`Gas Chromatography Troubleshooting course, Hewlett—Pachard, Atlanta, GA, September 27,
`1987.
`
`introduction to CALS Course, R.;l. Reynolds Toibaeco Company, Winston—Salein, NC,
`February 14, 1986.
`
`Aquarius 'Tl‘i‘aining Course, 1-iew1ett~1’ac1<ai'cl, New Orleans, LA, Septernltrer ll, 1987.
`
`RJR Mahageinent Developnieiit Prograin Course, RJ. Reynolds Tobacco Coinpany,
`Winston~Salein, NC, lune 27, 1988.
`
`VG Instruments, 70s and 7OSE Basic Operation, Savannah, GA, October, il98S.
`
`Vi} lnstrurnents, Quantitative Trace Analysis, Savanna.h,
`
`(SA, November, l988.
`
`Beclrinan 1’ea1<—1’ro for l3>;perienced Users, V\"i1’1StO11-38.16111, NC, November, 1988.
`
`Who's—Who in Science Ett’1(lEI1gi116f3-1” in 1992.
`
`National Urban Leagues Black Executives Excliarige Program (BEEP) 5 Year Service
`Award, 1995.
`
`RJR Project Leader 'Tl‘i‘aining Prograrn, 1’eln‘uai‘y, 1992.
`
`Central North Carolina Section, ACS Presitlent"'s Avvarct, 1994.
`
`G. Robert Di1\/laico R&1) Team Award for Results Through Diversity Facilitatiori, 1995.
`
`Central North Carolina Section, ACS, Outstanding Service Avvarct, 1995.
`
`Metliation Services of Foi‘syth County, Past~Chair Awairl, l998
`
`Winston—Sa1ern Staite University/RJR Partners Prograni Mcntor—of—the—yea.r Award, 1998.
`
`I I
`
`IIO
`
`U I
`
`
`
`IIOIIIOIOI
`
`lnstitutes, ‘Workshops anti Conventions Attended:
`I
`
`Mississippi Section of The Anicrican Chemical Society, 1971 ACS Filrh Short Course,
`llattiesburg, MS.
`
`Toxicology Worksliop, Houston TX, 1972
`
`Pittsburgli Coriferenee, Clevelaritl, Oil, Atlantic City, NJ, 1973, l977, 1979, 1988.
`
`Gas Chromatography Short Course, Washington University School of Continuing Education,
`St. Louis, MO, 1973.
`
`1-lewlett~1’acl<ard Gas Chroinatography' Tro1.thleshooting course, Atlanta, (SA, 1974.
`
`Liquid Chromatography Worlrsliop, Waters Associates, l‘vitilfoi"rl, MA, 1975.
`
`0000010
`
`5
`
`0000010
`
`

`
`{CV Cont’d)
`
`EARL L. W'H1'"1"1?.
`
`Southern Association o1’17orensic Scientist Meeting, Albnrn, AL, 1975
`
`Mississippi AcaC1ernyot‘Science Convention, ,1aclrson, MS, 1975
`
`Mass Spectral lnterpretation Course (Fred 1\/lclaaffertyfi, Raleiglr, NC, 1978.
`
`North Carolina Academy of Science, Greensboro, NC, 1979.
`
`Arnerican Society for Mass Speetronietry, Minneapolis, MN, Honolulu, H1, San Antonio,
`TX, San Diego, CA, Cincinnati, 011, Denver, CC’, San Francisco, CA, Miarni Beach, 17L,
`'1'ucson, AZ, Washington, DC, Atlanta, GA; 1981, 1982, 19S4, 1985, 1986, 1987, 1988,
`1989, 19911, 1992, 1995.
`
`National Teclinical Association National Convention, 1<.issiinniee, 171..., Cleveland, 011, 1983,
`1984.
`
`l111,1‘0(.ll!C'l,1OI1 to CALS Course, R1. Reynolds Tobacco Coinpany, Vi’7111S'lC111—S21.1i?1T1,NC, 1986,
`
`1<,epnei‘—'Tl‘i‘egoe Rational
`Winston—Salern, NC, 1986.
`
`Pr'oeessiiig
`
`course, R1 Reynolds Tobacco Company,
`
`1'levv1ett—Pac1<ard ,Aquarius Training Course, New Orleans, LA, 1987,
`
`VG instruments 70S Basic Operation Training Course, Savannah, GA, 1988.
`
`Vi} lnstrurnerits Qua.ntitative Analysis Course, Savanriali, GA, 1988.
`
`international Worlrsliop on Hybrid Mass Spectrometry, Lake Louise, Canada, 1988.
`
`Arnerican Cliernical Society National Meeting, Washington,, DC, New Yor'1<, NY, Denver,
`CO, Chicago, 11,, San Diego, CA, San Francisco, CA, Oriaiirlo, FL, Las Vegas, NV, Dallas,
`TX, San Francisco, CA; 1990, 1991, 1992, 1993, 1994, 1995, 1996, 1997, 1998 and 1999.
`
`Several Statistics Courses (1989, 911) and several others.
`
`United States Army Reserve Annual Traiiiing, Medical Service Corp, Ofticei"s Basic and
`Advance Courses, Ft Sam Houston, TX, 1989 and 2003.
`
`United States Army Reserve Annual Training, Medical Service Corp, Willi-ani Beaumont
`Arrny lviedical Center (Chief, Chemistry Lab Training), E1 Paso, TX, 1990, 1991, 1992, 1993
`and 1994,
`
`United States Army Reserve Annual '1‘raining, lviedical Service Corp, Armed Forces Medical
`intelligence Center (Analyst, NBC), Ft. Detrick, MD) 1995 & 1996.
`
`United States Army Reserve Annual Training, Medical Service Corp, Defense lntelligence
`Agency (fD1A} (Sr. Analyst, NBC), Boiling A1378, Washington, DC, 1997, 1998, 1999 &
`20110.
`
`innovation Week Training, R.l R.1'1R Departnient, Wi,nstori~Salern, , (1993).
`
`Creative Leadership Training, Center for Creative Leadership, Greensboro, NC, (1993).
`
`United States ,Army Reserve Annual Training, Medical Service Corp, Armed Forces 1‘v1edical
`intelligence Center (Senior Analyst '1‘i'aining}, 1’t. Detrick, MD, 1995 and 1996.
`
`United States Ariny Reserve Annual Training, Defeiise lntelligence Agency, D1A (Senior
`Analyst Tr'airiing}, Wasliingtoii, DC. 1997.
`
`"1‘o'l7acco Cliernist Research Conference, Greensboro, NC, Winston—Sa,1ein, NC, (19911, 1997).
`
`I I
`
`OCI
`
`I Q
`
`IOIO
`
`I
`
`0000011
`
`0000011
`
`

`
`{CV Cotitki)
`Piitilicatinns:
`
`EARL L. ‘WH l’"l"E
`
`l.
`
`5»:
`
`DJ
`
`1..., Development of Gas Chroinatograpliic Techniques for the Analysis of
`iwhite,
`Toxaphene, Chlorofoini and Hexa.cliloi'oethane". l\/LS. Thesis, (£975).
`IRCS
`Hume, A. S., Douglas, B. H. and White, E. L, Placental transfer of ethyl alcohol.
`.71/Zea’. Sci: Drug ll/feiczb. anti Taxical. Pimrim:zc0l., P!zysioi.,* Repmd, Biostretic asmd Gym, 4:
`203 (l 976).
`M. and Plaultey, F. W. Flame
`‘White,
`L, Zi6ll‘(lSl:\"i, C. A., Port'.z'ei'. R.l‘°~/'l., lleitliinar,
`emission studies of S€‘FV€l”Etl
`nietal chlorides with vapor phase sampling.
`/final. Cizim. /iota,
`ltll: 89-97, (l 978’).
`White, E. L, Bus, 3. S. and l'i€t3i{,
`
`l-E.
`
`ii'A.
`
`Siniultaneous tleterinination of n~llexane,
`
`tissues by gas
`in biological
`antl 2,5—lie>;aneclione
`2—l1exanone
`speettotnetry. Biomed. M52155 Specz‘r0i:n., ti:
`l69-l72, (E979).
`Bus, J. 8., ‘White,
`L, Tyl, R. W. and Barrow, C. S.
`l?’ei"inatal toxicity and nietabolisni of
`n—liexane in Flscllfil‘-34'-l rats after inlialation exposiiie during gestation.
`Toxicoi.
`/lppi.
`P.i’/zai"mac0f., 51: 295-302. (l979).
`
`clironiatograpliy—niass
`
`Gillies, P. J., Norton, R. lvl, ‘White, E. L. and Bus, 1. S. Studies on the inhibition of
`
`sterologeiisis in ll6X3.CEt1‘l3Gl'1—lti(.lllC§3Cl. distal axoiiopatliy in scia‘tic nerve of the rat.
`/tppi. _P!zamza(:0i., 54: 2l7~22'2, (l 980).
`lrons, R. D9,, Gross,
`A. and ‘White, E. L. At’lliit'1€-.2 Eviderice for an euterogastric cycle in
`the rat. Food C0.s':inez. Toxi(:oi., 1%: 393~39'7, (l98tl‘).
`Bus, 3. S., White,
`1..., Gillies, P. J. and Barrow, C.
`
`Tissue distribution of t'1—l16Xat'1€-,
`
`T(}.it”i£'z?:l.
`
`nietliyl n—butyE l<etone and 2,5~liexanedione in rats after single or repeated inhalation
`exposure to n—lieXane. Dmg M'el‘a1'). Disp0.<:., $3‘: 3S6~38'7, (l9Sl).
`l-leck, l-E. d‘A., ‘White, E. It... and Sclirnitz, M. C. Determination olfornialcleliyde in biological
`tissue by
`eln'oi'natogiapl1y~inass spectionietry. Birzined.
`1‘/2’ic2.s'.s‘ Specz‘r0m.,
`$3‘: 347-353,
`(1982).
`An
`L, Clieplen, J. M., Sherrill, J. M. and Haniin, JR, T.
`‘White,
`Raynoi‘, T.
`evaluation of a. water purification systein for use in animal facilities. La:zl70rat0.ry Animals,
`18: 4561, (i984).
`
`10.
`
`ll.
`
`l2.
`
`Cheplen, J. M., Barrow, C. S. and ‘White, E. L, Deteiniination of di1’l301‘11t) liee elilorine in
`the presence of aniinonia by capillary gas ehminatography. Anal. Chem., ‘S6:
`il94—ll96,
`(l.984‘).
`lt/l., Reaction of aninionia with
`llass, J. R. and l3ui*sey, M.
`.l~t'_3.,
`‘White, E. E... Tabet,
`accelerated benzoyl ions using triple qi.iadri.ipole niass specti‘oineti"y. Org.
`.M'us.~: Spectrmn.
`22, l32—l39 (1987).
`ions:
`.White, E. E... "Reaction of annnonia with gas-phase plienyl benzo-ate and benzoyl
`lon—niolecule reaction study using triple quadrupole niass Sp€Cll”0l‘I1€’t1‘:y’".
`Pli.D. tlisseitation,
`G987).
`
`l4.
`
`7
`
`(Bordon, M., B0t'g6I'Clil'1g_. M. F., Chung, H. l..., Coletrian, W. M., lll, Elder, J. F., Giles, J.
`A., lvloore, D.
`Rix, C.
`Ulirig. l\/l.
`anti ‘White,
`1.. Analysis of ilue—curecl tobacco
`essential oil by l'1§/pl,1f31'1?ll(;‘(l analytical teeliniques. J. Chro.inc:z.f.. Sci., 25., (1988).
`.VVl‘ti‘l.'63,
`l... and l3ui‘sey,
`l\/l.
`l\/l.,
`Distinguisl'iiiig 1')0Sil.lG1'1al
`isomers of liexachloriuated
`biplienyls by ion—inoleeule reactions in a triple~o,uacli'upoEe instrument. Biomed. Erzvirrm.
`1‘/2’a.<:s Specfrmn.
`l8, 4l 3-415, (l 989).
`
`0000012
`
`0000012
`
`

`
`EAR1. 1;. ‘WH1‘"1"1?.
`
`{CV Coiitki)
`16.
`.1. S. Effects of niethyi etny1 ketone on hepatic
`.111, 13., ‘White, 131. 1.. and 1?»ns,
`RO1‘}61‘1S01’1,
`niixed-tiinctioh oxidase activity and on in Vl11»’0 niet-a13o1isni of :4-hexane.
`.1’é2n()i)i(3lic:'a,. 1.9,
`721-729, (19897).
`. ‘W1i1te,
`14., Uhrig, M. S., Johnson, '1". 1., Gordon, B. M., Hicks, R. 1)., Borgerding, M. 13.,
`Coienian, 111, W. M. and Eider, .1r.,
`.1. F. Quantitative d.eterinina.tion of seieeted eoinponnds
`in a K.en1ue1<y 1R4F eiga.rette smoke by inu1ti<1in’iensiona.1 gas e1irornatograp1iy and se1eete<1
`ion menitoring-mass spectrometry,
`.1. Clzronzaragzi‘. S<:'i., 28, 393-399, ( 199(1).
`C1111, 1., Bodnar, .1. A., Bownian, R. N. and ‘White,
`1.. Deterniination ofviricristiiie and
`
`17
`
`18.
`
`19.
`
`vin131astine in Caihz;zrc2nl‘1zus rosezm pi-ants by high perfoiinance 1iquiC1 chroinatography/’
`e1eetrospray ionization mass specti‘on'1et1”y.
`.1’. Lia}. Ci*zmmaz‘agr. <1’; Rel. ‘fieeiznoi. 211$) 1 159-
`117-1 (1997).
`Cnu, 1., Botinar, .1. A., Bowman, R. N, 21111.11“/"1'l1t¢B, E. L. Quantification ofvineristine and
`vinb1astine in C'z:zt}u;:zraz'zzi2us roseus p1ants by eapi11aryzona.1 e1eetrop11oresis. J. of
`Ciiromafogr. /1. 755 281-288 (1996).
`Che, 1-H. and 1/’1«’1i1§fe, E. 1..., On-1ine Focusing CE-MS DC1.f3.1‘1111I1E.11011 of v‘inb1astine and
`vincristine, 1n preparation, (1998).
`.C1iu, 1-11., Munzo, .1. A. and ‘vV1i1te, E. 1...,
`
`A ca:pi11ary e1ee1:rop1ioi'esis,/niass speetroinetry
`niethod for the deterrnination ot‘to13aceo specific nitrosarnihes with eieetrospray ionization
`detection, 1n preparation, (1998).
`. VV1§11€., E. L and Brooks, C. 0., Deveiopment of an on-eo1u1nn cryogenic trapping method
`for the c1et.ei'niination of vinyi enioride in iiresh mainstreain vapor phase eigaretite smoke, 1n
`pi'eparation , ( 1998).
`.. 1-1aniey, K., Nguyen, 1..., Khan, 13., Pogne, (1., Vojdaiii, 13., Panda, 8., Pinot, 13., Oriedo, ‘v’.,
`1{asoc1'1ova, 1..., Siibraniaiiian, S., 1‘v1i11er, 13. and ‘White, 131. 1... Deveiopinent of a piant vira1-
`vector—1‘.>aseC1 gene
`expression assay for
`the
`screening of yeast cytochrome P450
`inonooxygenases. z1.ssaya'm?iDrug‘development Tecr’2rz0i0g‘ies. 1(1-2) 147-160 (20037).
`Ba, 11., Cameron, '1‘. L, Gargei‘, S. 1., Pogne, G. 1)., Hamm, 1;. A., ‘White,
`Haniey, K. M.
`and Gra1:>ows1<:i, G. A., Woinian disease/e11o1estery1 ester storage disease: efficacy o1"p1ani.-
`produced hnnian iysosoniai acid iipase in mice. J. Lipid Res. «19, 1646-1657 (2008).
`1\) ‘J:
`., 1\I1€.‘.C01'I“1‘11C1§, A. A., Rei1dy,S., R.ein1, S. 1., Cameron, 1). 1., CZ61‘W’11'11x“S,K1, D. K, Vojdani,
`1~1an1ey, K. 1‘~/1., Garger, S. .1., ‘White,
`1..., Novak, 1,, Barrretii, 1,, 1»1o1tz, R. 15., Tuse', D. and
`1..etvy, R. 1’1aiit-priaducetl idiotype vaccines for the treatnient of i'1on-11or1g1<in’s 1ymp1'1on'1a:
`Safetyt and ininninogenicity in a phase 1 eiinieai st1.idy.
`’N/15 1115-(29) 1131 31--111136 (213118).
`Zoi.i1<:hri, 1)., Rawe, 1., Singh, 1V1}, Kub1in, C., Dawson, K., 1-iarldoh, W. 17., ‘White, E. 11.,
`Haniey, K. M., Tuse, 1)., Maiyj, W. and Papas, A. Discovery of Putative Saiivary Biomar1<ers
`for S_i'ogi"en’s Syiidronie Using High Resointion Mass Spectrometry and Bioinforrnaties,
`Subniitted, (20119).
`. Pogiie, (1.15, Voj dani, F, 1’a1n'1ei', 1{.1Ei., 1-iiatt, 131., 1-1unie, S., Piieips, 1., Long, 1..oi'i, Bohorova,
`N, Kim, Do, 1’aii1y,
`1*»/1., Ve1asco,
`.11., Wnaiey, K., Zeitiin, 1..., Garger, S.
`.11., White,
`141.,
`iiaydon, 11. and B1”fl1C11€1', 3., 1“i‘ot1nction of 1’1iai‘inacentiea1-grade reeonibinaiit aprotinin and
`a nionoeionai antibody product using piant-isased transient expression systems.
`Picmt
`Biozeci/.'.n0:.’0gyJournal. 8, 1-17, (2010).
`
`26.
`
`0000013
`
`0000013
`
`

`
`{CV Coritkl)
`Abstracts anti./or l‘*“resentatinns:
`
`EARL L. Vi/H i’"i"E
`
`l.
`
`is
`
`Lu.)
`
`L, Participating Lecturer, Pre-suninrer lnstitute on lnstrurnental Methods,
`‘Whites
`Jackson State College, Jaclison, MS (May l975).
`Planlsey, F. W. and ‘Vt/liite, E. L. A niieroeoinputer controlled atoniie einission systein.
`l?,asten'i Anal. .Ԥyii'ipos.. ( l 977).
`Bus. .l. S., ‘White,
`14..
`l%leel<, ll. d’A. and Gibson. 3. i3., The distribution and n'ieta‘oolisni
`
`ofn~liexarie in pr'egriant Fiscller~344 rats. Te:mz'r)iogj.r, l7:42A, (l 978).
`Bus. J. 8., Vt7i’lli€:,
`L. and Barrow. C.
`Disposition of n-l_iexa.ne in rats alter single and
`repeated inlia.lation exposure. Toxicoi. Aflopf. Phc:zrzlna«:r).i.., 48: Al67, (l 979).
`White: E. l... and Bus. 3. 8.,
`clirorna.tog;'apliic-rnass spectral analysis n~liexane, nietliyl
`ethyl lretone and their metabolites. Nortli tiiarolina, Acaderny of Science, t3reensboro. Ntii,
`(E979).
`Gillies.
`
`l’.
`
`.l., Norton, R.
`
`l\/i., ‘White,
`
`L. and Bus, 3. 8., Studies on the inhiliition of
`
`Sterolgenisis in llC){E,C§il‘l3011~l11<;l.llCCtl distal axonopatliy in sciatic nerves of the rat. Soc. of
`Toxicol. (lillitl).
`
`Detern’iiriation of n—liexarie, rnetliyl etliyl lretone, and their
`‘White, E. E... and Bus, 3.
`metabolites in sciatic nerves of rats by gas elironiatograpny»rnass spectrometry. S.At§Ii’~SSl’
`l’ittsliurgl1 Conference, U980).
`
`Baker, T. 8., ‘Wliite,
`L. and Rickert D. E., IDiffei‘ences in the nietabolisni of n—lrexane in
`
`Fiscl1ei'—3r-M rats and C57Bl nnce. ASPET, (1980).
`W’liite: E. L. and Riclrert, D. E, Metaloolisin and disposition of inhaled Zmbutanone in rats
`deterniined by gas chrornatograpliy~niass spectron’ietry. 29%‘ Annual Conference on Mass
`Spectrometry and Allied Topics. {ASl‘vlE§), 541, (l 98li).
`Heck, E-l. d'A., ‘White:
`iii. EA: and Selirnitz, M. C., Determination of foi‘inaldenyde in
`biological tissue by gas cln‘onrato_grapliy—ina.ss spectrometry.
`30th Annual Conference on
`Mass Spectroinetry and Allied Topics. (ASMS), 32, (1982).
`‘Willie:
`L. Heck, H. (FA. and Clieplen. J. l\