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`45) ISSUED
`
`10407
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`260-497
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`(51)
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`assmnaafs
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`Ffiifii mzscmmmis
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`ggzwmbeimy lsipidra and «:m‘.1%:37.n awmprirma amuhaifimxtimm sf 1*»-
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`maxim maxi from “him ~?w~a.w—1- mam prnsn-t:e;1.n£e are mztmss had
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`firwm fine
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`at a pi’. ofi 10 to 12. Tim alkaline erztvamt;
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`cuff prazsteineza is
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`f protein of
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`AKER8771TC00068709
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`AKER8771TC0006871 0
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`10638906
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`mmmmglmw are yrmxcnfiud below.
`
`Examplm 1
`
`in an myparmtma wink a wfigaaimy m& 10 i pmwvidwd wimh
`
`a fitirfifif thwra 13 placafi
`
`1 kg wfi krill wflimh ia fillmfi with
`
`watwr and stirrwa at &,$$0 ppm fmr 0.3 hmmr“
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`Thw rwxultanfi
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`mmulsimm mf iipidg £3 fiwynratmd frmm thm kriil mafia by
`
`iiltraaiom mhrmmgh a gtuinlwfia staal gwrmmm havififi 3 m@$h $i3@
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`mi 1x1 mm.
`
`The krill mama iw tnanfifuxrwd t& the ucwawl with M
`
`atirrar intm which fiherm 13 addafl
`
`3
`
`1 05 an aquawufi xwlufiiom
`
`10
`
`mf Nafifl mi $umh a mmncfintratiwn ag hm r@&mh & pfi $5 the mixtur$
`
`Qf 10 fihd fitixrfld £0: half an hwur. when
`
`Krill
`
`fig trwmtfifl
`
`with alkal& wxtrfimaxwn Qf prwtwinx tékflfi pémmm.
`
`Tha rafiultamt
`
`axtracn wk prmfiainm ifl fieparanad firmm thw im$m:um1e £m$ifiu@ mi
`
`chitin imtagumemtw my filtratimn bhrmmgh a m&t&l $ar$$n with a
`
`maah fiixa 0;
`
`iml mm and mwntzifug@d at 2§$Gfl% rpm far $.15 hmur
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`aw Eemmvm imgurifiima.
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`Tm the puKifi&d &xt£a¢t wf prmtwins thara
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`ifi afldafl whiiw fitirring a
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`lwmola fiolmhimn Qf MGR $0 r®&wh & gfi
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`mi 4.5, prumwin bwing premipitotedfi
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`Tha prwmé§itmt& £3 lmlt
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`hm fiattim for 3 hwuxa, bharwaihar ih ia s&yar&h@& frmm thw liguifi,
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`waahma with 3
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`Iitmrw mfi watwr and firiefi
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`:yQ§hi1iG&]1ym ?me
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`pxmteiu pxmfluct mbtainafi
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`im an ammmnt m£ $0 g ifi a §&lQW@iflk
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`wdmrlwaa pwwderg having a mwistmre momtmnt $5 30% by weight
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`fififl
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`mmmpxifiing 85% by wwigha«3fpxm&ein&uu}2L my wuight mi
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`lipifia.
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`THE rexifluw Qi krill Qrmduaafi a§u&x fieparatiwn mf
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`thw mmtxact mi prateina ia pre33afl
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`to xammvm mmiatura amfi drigé
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`ta grmducm 1? g Qf ahitin intagumants,
`
`Exampla 2
`
`Thw prwaefigxhq at krill &m mavriwfi amt
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`in the $am%
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`mannar a3 in mmamplm 1, axmapk Chfit amul§ifim&timm mf
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`lépiflfi
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`ix Qomaumtafi
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`in a 001% mola aqumwug fiolmaiwn wf $mdium
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`wulmrifi& at a pH mf &.5«
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`The protein prmflumt mhtainwfi
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`in an
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`ammunt of SR g hafi a mwi$aux& amnfiemu wf
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`33% anfi womprigmg 80%
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`my wcight of yxwtaim ané 3% by wmighh m{ }ipié$: and 20 g 0f
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`AKER877|TC00068716
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