CURRICULUM VITAE
`
`Dennis J. Thiele
`
`Department of Pharmacology and Cancer Biology
`Duke University Medical Center
`Research Drive, LSRC - Room C134
`Durham, North Carolina 27710
`Phone: 919-684-5776
`e-mail: dennis.thiele@duke.edu
`
`July 25, 1956, Syracuse, New York
`
`ADDRESS
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`PERSONAL DATA
`
`Born:
`
`
`Married:
`
`
`
`Maria Sippola-Thiele, Ph.D., M.B.A.
`children:
`John 22 (Senior, Ursinus College, Collegeville, PA)
`Katri 19 (Sophomore, Dickinson College, Carlisle, PA)
`
`
`EDUCATION
`
`Penfield High School, Penfield, New York, graduated 1974
`High School:
`
`State University of New York, College at Fredonia, New York,
`Undergraduate:
`1974-1978, B.S. (Biology) Laboratory of Dr. Valgene Dunham
`
`Rutgers University, Rutgers Medical School,
`
`Graduate:
`New Brunswick, New Jersey, M.S., 1981, Ph.D. 1983
`
`
`
`(Microbiology) Laboratory of Dr. Michael J. Leibowitz
`
`
`
`
`POSTDOCTORAL TRAINING
`
`Staff Fellow, Laboratory of Biochemistry (Chief, Maxine Singer)
`National Cancer Institute, Bethesda, Maryland 1984-1987
`
`ACADEMIC APPOINTMENTS
`Assistant Professor of Biological Chemistry, The University of Michigan, Medical School, July
`1987; Associate Professor, 1992; Professor, 1996-2003
`
`George Barth Geller Professor, Department of Pharmacology and Cancer Biology, Duke
`University Medical Center, August, 2003-present (Vice Chair, 2006-2012)
`
`
`
`
`
`1
`
`

`
`
`
`
`
`CONSULTING POSITIONS
`1990-1994, Apex Bioscience, Durham, North Carolina
`1994-1996, ApoLife, Inc., Scientific Advisory Board, Detroit, MI
`1999-2000, Merck and Company, West Point, PA
`2009-present, Chaperone Therapeutics, Research Triangle Park, NC
`
`EDITORIAL BOARDS
`
`The Journal of Biological Chemistry 1996-2000, 2001-2005
`Genes and Nutrition: 2006-present
`Eukaryotic Cell: 2011-present
`Virulence: 2011-present
`
`GRANT SUPPORT
`Active
`
`
`
`Sponsor:
`
`
`Period:
`
`
`Title:
`
`Principal Investigator:
`
`
`
`
`Sponsor:
`
`
`Period:
`
`
`Title:
`
`
`
`Principal Investigator:
`
`
`
`Sponsor:
`
`
`Period:
`
`
`Title:
`
`
`
`Principal Investigator:
`
`Sponsor:
`
`
`Period:
`
`
`Title:
`
`
`Principal Investigator:
`
`Pending
`
`
`
`Sponsor:
`
`
`Period:
`
`
`Title:
`
`Principal Investigator:
`
`
`National Institutes of Health (R01 GM41840-24)
`7/1/09 – 6/30/13
`Metal Homeostasis in Yeast
`Dennis J. Thiele
`
`National Institutes of Health (R01 DK074192-11)
`8/1/11-7/31/16
`Copper Homeostasis in Mammals
`Dennis J. Thiele
`National Institutes of Health (R01 NS065890-03)
`4/1/10-3/31/14
`HSF1 as a therapeutic target in neurodegenerative disease
`Dennis J. Thiele
`National Institutes of Health (R21 AI106013-01)
`4/1/13-3/31/15
`Mechanism for copper-deficiency mediated neutropenia
`Dennis J. Thiele
`
`National Institutes of Health (R01 GM41840-25) competing renewal
`7/1/13 – 6/30/18
`Metal Homeostasis in Yeast
`Dennis J. Thiele
`
`
`
`2
`
`

`
`PATENTS:
`“Novel Yeast and Mammalian Regulators of Cell Proliferation”
`Patent Number: 6,383,753 Issue Date: May 7, 2002
`Dennis J. Thiele and Phillip C.C. Liu
`
`“Compositions and methods relating to Heat Shock Transcription Factor Activating Compounds
`and targets thereof”
`Dennis J. Thiele and Daniel W. Neef (Pending)
`
`CERTIFICATION AND LICENSURE
`None
`
`MILITARY SERVICE
`None
`
`HONORS AND AWARDS
`1974-1978
`- New York State Regents Tuition Scholarship
`- Frank G. Brooks Award (Tri-Beta) for Excellence in Undergrad. Research 1978
`- Theobald Smith Award, New Jersey Ch. Amer. Soc. for Microbiology)
`1983
`- Damon Runyon - Walter Winchell Cancer Fund Postdoctoral Fellow
`1984-1986
`- Burroughs Wellcome Toxicology Scholar Award
`1994-1999
`- University of Michigan Faculty Recognition Award
`1997
`- University of Michigan Distinguished Faculty Achievement Award
`2001
`- Vice Chair, “Stress-Inducible Gene Expression” Gordon Conference
`2003
`- University of Medicine and Dentistry of New Jersey Graduate School
`
` of Biomedical Sciences Distinguished Alumni Award
`2005
`- Chair, Stress Proteins in Growth, Develop, Disease Gordon Conf.
`2005
`- Rutgers University Graduate School Distinguished Alumni Award
`2006
`- George Barth Geller Professor of Pharmacology and Cancer Biology
`2006-present
`-Inaugural Arturo Leone Lecture, Intl. Meeting on Copper Homeostasis
`2006
`- Elected Fellow, American Association for the Advancement of Science
`2006
`- Elected Fellow, American Academy of Microbiology
`2007
`- Co-Organizer with Mary Lou Guerinot, FASEB Summer Research
` Conference on Trace Element Micronutrients, Snowmass, Colorado 2008
`- Keynote Lecture, Cell Biology of Metals Gordon Conference
`2009
`- Keynote Lecture, Stress Proteins Gordon Conference
`2009
`- Keynote Lecture, FASEB Trace Elements Summer Conference
`2010
`- David Danks Award Lecture, International Copper Meeting
`2010
`- Outstanding Alumni Award, SUNY College at Fredonia, N.Y.
`2012
`- Keynote Lecture, Human Disorders of Copper Metabolism Meeting
`2012
`- Vice Chair, Cell Biology of Metals Gordon Conference
`2013
`- Chair-Elect, Section on Biological Sciences, American Assn. Adv. Science 2013
`- Co-Organizer, International BioMetals Meeting
`2014
`- Co-Organizer, 9th International Copper Meeting
`2014
`
`
`
`3
`
`

`
`2015
`
`- Chair, Cell Biology of Metals Gordon Conference
`
`MEMBERSHIPS AND OFFICES IN PROFESSIONAL SOCIETIES
`
`
`Genetics Society of America
`American Society for Microbiology
`American Association for the Advancement of Science
`American Society for Biochemistry and Molecular Biology
`
`
`TEACHING ACTIVITIES
`
`
`University of Michigan Medical School (1987-2003)
` Biological Chemistry 522, Spring 1988 (Biochemistry for Dental Students)
`- Coordinator, Genetics Training Grant Student Seminar, Winter, 1990
`- Course Director, Biological Chemistry 416/516, Fall1988-1991(Undergrad. Laboratory
`course)
`- Course Director and Lecturer, Biological Chemistry 571, Fall, 1992-1998
`
`(Nucleic Acid Structure and Dynamics)
`- Biological Chemistry 572, Fall, 1992-2003
`
`(Biochemistry of Gene Expression)
`- Biological Chemistry 578, Fall, 1992-1999
`
`(Biochemistry Research Techniques)
`- Biological Chemistry 700, 1996-present
`
`(Critical Analysis of the Literature)
`- Course Director, Biological Chemistry 550, Fall 1999-2003
`
`(Protein Structure and Function)
`- Course Director, Biological Chemistry 682, Spring, 2000, 2001
`
`(Biochemistry of Gene Expression)
`- Engineering 100: (Intro to Engineering), Fall, 2000
`- Biology 401, Current Topics in Biology, Spring 2001
`
`Duke University
` Co-course Director with Xiao-Fan Wang, Pharmacology/Molecular Cancer Biology 280, 2003-
`present
`- Course Director and Lecturer: Cell Biology of Metals, 2008-present
`- Course Director and Lecturer: Protein misfolding and disease, 2011-present
`
`
`
`COMMITTEE AND ADMINISTRATIVE SERVICES
`
`University of Michigan Medical Center (selected, over 16 years)
`
`
`- Department of Biological Chemistry Chairʼs Advisory Committee, 1988-1992, 1995-2003
`
` -
`
` -
`
`
`
`4
`
`

`
`- Faculty reviewer, Michigan Minority Merit Fellowship Competition, 1989-1995
`- Faculty Search Committee, Department of Otolaryngology, 1990-1991
`
`
`- Steering Committee, Univ. of Michigan Cellular Biotechnology Training Program, 1991-2003
`- Faculty Search Committee, Dept. of Biological Chemistry,1992-1995
`- Faculty Search Committee, Department of Biology, University of Michigan, 1992, 1993, 2001.
`- Member, Scientific Advisory Board, University of Michigan-Warner-Lambert/Parke-Davis
`Postdoctoral Training Program, 1992-1996
`- Graduate Student Recruitment and Admissions Committee, Department of Biological
`Chemistry, University of Michigan Medical School: 1992, 1993 (Co-chair), 1994 -1998 (Chair)
`- Undergraduate Summer Research Program Committee, Dept of Biological Chem.,1993, 1994
`- Organizer, Dept. Biological Chem. Research Retreat, University of Michigan, 1992-1995
`- Organizer, Department of Biological Chemistry Seminar Program, 1993-1996
`- Member, University of Michigan Medical School Biomedical Research Council: Deanʼs
`Advisory Committee, 1996-1999
`- Founding Organizer with Ronald Taussig, Dept of Biological Chemistry Postdoctoral Seminar
`Program, 1997-2000
`- Organizer, the University of Michigan Life Sciences Lecture Series, 1999-2001
`- Chair, Univ. Michigan Medical School Biol. Chemistry Faculty Search Committee, 2000-2001
`- Co-chair with Carol Fierke, University of Michigan Joint Life Sciences Search Committee,
`2000-2001
`- Member, University of Michigan Medical School Department of Biological Chemistry Chair
`Search Committee, 2002
`- Member, University of Michigan Medical School Medical Scientist Training Program
`Operating Committee, 2001-2003
`- Member, Interdepartmental Committee on Recruitment and Selection of Biological Sciences
`Scholars, University of Michigan, 2001-2003
`- Curriculum Advisor for graduate students in the Department of Biological Chemistry,
`University of Michigan, 2000-2003
`
`Member:
`Chemistry-Biology Interface Training Program
`Genetics Training Program
`Cellular Biotechnology Training Program
`Toxicology Training Program
`Cell and Molecular Biology Training Program
`University of Michigan Comprehensive Cancer Center
`University of Michigan Diabetes Center
`
`Duke University (selected, 2003-present)
` Member, University Program in Genetics and Genomics
`- Member, Cell and Molecular Biology Program
`- Member, Interdepartmental Toxicology Program
`
` -
`
`
`
`5
`
`

`
`- Chair, University Program in Genetics and Genomics Student Admissions, 2004-2008
`- Chair, Dept. Pharmacology and Cancer Biology Faculty Awards Committee 2004-present
`- Co-chair with Danny Lew, University Genetics and Genomics Seminar Program
`- Chair, Pharmacology and Cancer Biology Faculty Search Committee, 2007
`- Chair, Duke Institute for Genome Sciences and Policy 5 year review committee, 2008
`- Chair, Duke University Biochemistry Chair Search Committee, 2008-2010
`- Member, Duke Univ. School of Medicine Appointments/Promotions Cmt. 2005-2012
`- Member, Duke Univ. School of Medicine Conflict of Interest Cmt. 2010-present
`- Chair, Dept. Pharm and Cancer Biology Faculty Search Committee, 2010-2011
`- Member, Dept. Pharm and Cancer Biology Faculty Search Committee, 2011-2012
`- Chair, Dept. Pharm/Cancer Biol. Research Track Faculty Appts/Prom. Cmt. 2011- present
`
`
`National and International
` Reviewer, National Institutes of Health, National Center for Research Resources:
`Development of Non-Mammalian Models. 1992
`- Reviewer, National Institutes of Health, Special Study Section on the Possible Role of
`Metallothionein in Cancer. 1993
`
`- Reviewer, National Institutes of Health Postdoctoral Fellowship Applications, , BIOL-3 Study
`Section. 1994
`
`- Member, Molecular Biology Study Section (CDF-1, now MGB), National Institutes of Health,
`2002-present
`
`- International Oversight Committee, Trace Elements in Man and Animals, 2002-2009
`
`- Co-organizer with Drs. Anne Summers, Barry Rosen, Tom Curran and Max Costa, Keystone
`Symposium on Molecular and Cellular Biology : "Metal and Oxygen Regulation of Gene
`Expression", 1995.
` External Scientific Advisory Committee, University of Rochester National Institutes of
`Environmental Health Toxicology Center. 1996
` Scientific Advisory Board, Meeting on: "Copper Transport and its Disorders: Molecular and
`Cellular Aspects" Sestri Levante, Italy, May 21-25, 1997
` Organizing Committee, Second International Meeting on Metals and Genetics, Toronto,
`Canada, June 2-6, 1998
` Member, Scientific Committee, Fourth International Metallothionein Meeting, Kansas City,
`Mo, September 17-20, 1997
`
` -
`
`
`
` -
`
` -
`
` -
`
` -
`
`
`
`
`
`6
`
`

`
`- Co-organizer, Congress on Oxidative and Osmotic Stress, Netherlands, August, 1999
` Organizer, Seconding International Meeting on Copper Homeostasis and its Disorders:
`Molecular and Cellular Aspects, Ravello, Italy, September, 1999
` Organizer, Third International Meeting on Copper Homeostasis and its Disorders, Molecular
`and Cellular Aspects, Ischia, Italy, October 4-8, 2002
` Co-organizer, Midwest Metals Meeting, St. Louis, MO, May 16-18, 2003
` Vice Chair, “Stress-Inducible Gene Expression” Gordon Conference, Oxford, England, July,
`2003
` Organizing Committee, XXI International Conference on Yeast Genetics and Molecular
`Biology, Goteborg, Sweden, July, 2003
`
` -
`
` -
`
` -
`
` -
`
` -
`
` -
`
` Organizer, 4th International Meeting on Copper Homeostasis and Its Disorders: Molecular
`and Cellular Aspects, Ischia Porto, Italy, October 23-28, 2004
`
`- Chair, Gordon Research Conference on Stress Proteins in Growth, Development and
`Disease, Salve Regina University, July 2005
`
`- Co-Organizer, 5th International Copper Meeting: “Copper and Related Metals in Biology”,
`Alghero, Sardinia, Italy, October 14-18, 2006
`
`- Co-Organizer with Mary Lou Guerinot, FASEB Summer Research Conference on Trace
`Element Micronutrients, Snowmass, Colorado, 2008
`
`- Member, Wilsonʼs Disease Association Medical Advisory Committee, 2007-present
`
`- Ad hoc Member, NIH Macromolecular Structure and Function A Study Section, 2007
`
`- Reviewer, NIH NICHD Program in Molecular Medicine, December, 2008
`
`- Reviewer, Dept. Biochemistry, University of Nebraska, Lincoln, NE, 2011
`
`- Ad Hoc Member, Board of Scientific Counselors, NIEHS, Research Triangle Park, 2011
`
`
`
`
`
`
`
`7
`
`

`
`
`
`
`
`
`Ph.D. Students:
`
`-Pengbo Zhou (Professor. of Pathology, Cornell Medical School, New York, NY)
`-Katherine Tamai (Investigator, Dept. Anatomy/ Devel Biology, Univ. College London,)
`-Mark Szczypka (Vice Pres., Research and Development, Solohill Engineering, Ann Arbor, MI)
`-Keith Koch (Scientist, Cytoskeleton Inc, Westminster, Colorado)
`-Xiaodong Liu (Private Medical Practice)
`-Nicholas Santoro (Scientist, Great Lakes Environmental Program, Mich. State Univ.)
`-Hao Zhou (Data Analyst, New York)
`-Erin Rees (Research Asst. Professor, Univ. of Michigan)
`-Michele Turksi (Scientist, CollabRx, Palo Alto, California)
`-Sandra Vergara (Postdoctoral Fellow with Craig Mello, Univ. Mass.)
`-L. Kent Wood (Postdoctoral Fellow, Pioneer Hi-Bred, Iowa)
`-Alex Jaeger (current)
`-Brandon Logeman (current)
`
`
`
`
`Postdoctoral Fellows:
`
`-Dr. Geraldine Butler (Assoc. Professor, Dept. of Biochemistry, Univ. College, Dublin, Ireland)
`-Dr. Philippe Silar (Professor, Department of Genetics, University of Paris, Orsay, France)
`-Dr. Simon A.B. Knight (Asst Res. Professor, Microbiology/Immunol. Drexel School of Medicine)
`-Dr. Zhiwu Zhu (Professor, Dept. of Chemistry, Zhengzhong University, China)
`-Dr. Simon Labbe (Professor, Dept. Biochemistry, Univ. Sherbrooke Med. School, Quebec, CA)
`-Dr. Maria Marjorette O. Peña (Assoc. Professor of Biology, Univ. South Carolina, Columbia, SC)
`-Dr. Kevin Morano (Assoc. Professor, Dept. Microbiology, Univ. Texas Medical Ctr, Houston,TX)
`-Dr. Phillip Liu (Senior Scientist, Incyte Genomics, Wilmington, Delaware)
`-Dr. Jaekwon Lee (Associate Professor, Dept. of Biochemistry, Univ. of Nebraska, Lincoln)
`-Dr. Ji-Sook Hahn (Assoc. Professor, Dept. Chem/Biol. Eng., Seoul National Univ. South Korea)
`-Dr. Sang-Gun Ahn (Associate Professor, Dept. of Pathology, Chosun University, South Korea)
`-Dr. Sergi Puig (Asst. Professor, Dept de Biotec, Instituto de Agroquímica y Valencia, Spain)
`-Dr. Byung Eun Kim (Asst. Professor, Animal Physiology, Univ. Maryland-College Park)
`-Dr. Tracy Nevitt Goncalves (Asst. Professor, Dept. Biochemistry, Univ. Missouri Med. School)
`
`-Dr. Yasuhiro Nose-current
`-Dr. Daniel Neef-current
`-Dr. Chen Ding-current
`-Dr. Richard Festa-current
`-Dr. Helena Ohrvik-current
`-Dr. Maria Mar Martinez Pastor-current
`-Rocio Gomez Pastor-current
`
`
`
`8
`
`

`
`
`BIBLIOGRAPHY
`
`Completed Publications in Scientific Journals
`
`Peer Reviewed
`
` 1. Thiele, D.J., Wang, R.W. and Leibowitz, M.J. (1982) Separation and Sequence of the 3'-
`Termini of M Double-Stranded RNA from Killer Yeast. Nucleic Acids Research 10: 1661-
`1678
`
` 2. Thiele, D.J. and Leibowitz, M.J. (1982) Structural and Functional Analysis of Separated
`Strands of Killer Double-Stranded RNA of Yeast. Nucleic Acids Research 10: 6903-691
`
` 3. Thiele, D.J., Hannig, E.M. and Leibowitz, M.J. (1984) Multiple L Double- Stranded RNA
`Species of Saccharomyces cerevisiae: Evidence of Separate Encapsidation. Mol. Cell.
`Biol. 4: 92-100
`
` 4. Hannig, E.M., Thiele, D.J. and Leibowitz, M.J. (1984) Saccharomyces cerevisiae Killer
`Virus Transcripts Contain Template-Coded Polyadenylate Tracts. Mol. Cell. Biol. 4: 101-
`109
`
` 5. Thiele, D.J., Hannig, E.M. and Leibowitz, M.J. (1984) Genome Structure and Expression of
`a Defective Interfering Mutant of the Killer Virus of Yeast. Virology 137: 20-31
`
` 6. Hamer, D.H., Thiele, D.J. and Lemontt, J.F. (1985) Function and Autoregulation of Yeast
`Copperthionein. Science 228: 685-690
`
` 7. Thiele, D.J., Walling, M.J. and Hamer, D.H. (1986) Mammalian Metallothioneins are
`Functional in Yeast. Science 231: 854-856
`
` 8. Thiele, D.J. and Hamer, D.H. (1986) Tandemly Duplicated Upstream Control Sequences
`Mediate Copper-Induced Transcription of the Yeast Copper- Metallothionein Gene. Mol.
`Cell. Biol. 6: 1158-1163
`
` 9. Thiele, D.J. ACE1 Regulates Expression of the Saccharomyces cerevisiae
`Metallothionein Gene. (1988) Mol. Cell. Biol. 8: 2745-2752
`
`10. Huibregtse, J.M., Engelke, D.R. and Thiele, D.J. (1989) Copper-induced Binding of
`Cellular Factors to Yeast Metallothionein Upstream Activation Sequences. Proc. Natl.
`Acad. Sci. USA 86: 65-69
`
`11. Szczypka, M.S. and Thiele, D.J. (1989) A Cysteine-rich Nuclear Protein Activates Yeast
`Metallothionein Gene Transcription. Mol. Cell. Biol. 9: 421-429.
`
`
`
`9
`
`

`
`
`13.
`
`
`12. Evans, C.F., Engelke, D.R. and Thiele, D.J. (1990) ACE1 Transcription Factor Produced
`in Escherichia coli Binds Multiple Regions within Yeast Metallothionein Upstream
`Activation Sequences. Mol. Cell. Biol. 10: 426-429.
`Fujita, V.S., Thiele, D.J. and Coon, M.J. (1990) Expression of Alcohol - Inducible Rabbit
`Liver Cytochrome P-450 3a (P-450IIE1) in Saccharomyces cerevisiae with the Copper-
`Inducible CUP1 Promoter. DNA and Cell Biology 9: 111-118.
`
`14. Butler, G. and Thiele, D.J. (1991) ACE2: An Activator of Yeast Metallothionein
`expression which is Homologous to SWI5 Mol. Cell. Biol. 11: 476-485.
`
`15. Silar, P., Butler, G. and Thiele, D.J. (1991) Heat Shock Transcription Factor Activates
`Transcription of the Yeast Metallothionein Gene Mol. Cell. Biol. 11: 1232-1238.
`
`16.
`
`Zhou, P. and Thiele, D.J. (1991) Isolation of a Metal-Activated Transcription Factor
`Gene from Candida glabrata by complementation in Saccharomyces cerevisiae. Proc.
`Natl. Acad. Sci., USA 88: 6112-6116.
`
`17. Macheroux, P., Massey, V., Thiele, D.J., and Volokita, M. (1991) Expression of Spinach
`Glycolate oxidase in Saccharomyces cerevisiae: Purification and Characterization.
`Biochemistry 30: 4612-4619.
`
`
`18. Gralla, E.B., Thiele, D.J., Silar, P. and Valentine, J.S. (1991) ACE1, a copper-
`dependent transcription factor, activates expression of the yeast copper, zinc-
`superoxide dismutase gene. Proc. Natl. Acad. Sci. USA 88: 8558-8562.
`
`19. Silar, P. and Thiele, D.J. (1991) New Vectors for Direct Cloning in the Yeast
`Saccharomyces cerevisiae. Gene 104: 99-102.
`
`20. Saito, K., Thiele, D.J., Davio, M., Lockridge, O. and Massey, V. (1991) The Cloning and
`Expression of a Gene Encoding Old Yellow Enzyme from Saccharomyces
`carlsbergensis. J.Biol.Chem. 266: 20720-20724.
`
`21. Dohrman, P., Butler, G., Tamai, K., Dorland, S., Greene, J.R., Thiele, D.J. and Stillman,
`D.J. (1992) Parallel pathways of gene regulation: homologous regulators SWI5 and
`ACE2 differentially control transcription of HO and chitinase. Genes and Development.
`6: 93-104
`Thiele, D.J. (1992) Metal-Regulated Transcription Mechanisms in Eukaryotic
`Organisms. Nucleic Acids Research 20:1183-1191
`
`
`22.
`
`
`
`
`10
`
`

`
`23.
`
`Zhou, P., Szczypka, M.S., Sosinowski, T. and Thiele, D.J. (1992) Expression of a Yeast
`Metallothionein Gene Family is Activated By A Single MetalloRegulatory Transcription
`Factor. Mol. Cell. Biol. 12:3766-3775.
`
`24. Stott, K., Saito, K., Thiele, D. J. and Massey, V. (1993) Old Yellow Enzyme: The
`Discovery of Multiple Isozymes and a Family of Related Proteins. J. Biol. Chem. 268:
`6097-6106.
`
`Zhou, P. and Thiele, D.J. (1993) Copper and Gene Regulation in Yeast. BioFactors 4:
`25.
`105-115.
`
`26. Tamai, K. T., Gralla, E. B., Ellerby, L. M., Valentine, J. S. and Thiele, D. J. (1993) Yeast
`and Mammalian Metallothioneins Functionally Substitute for Yeast Copper-Zinc
`Superoxide Dismutase. Proc. Natl. Acad. Sci. USA, 90: 8013-8017.
`
`
`27. Zhou, P. and Thiele, D.J. (1993) Rapid Transcriptional Autoregulation of a Yeast
`
`Metalloregulatory Transcription Factor is Essential for High Level Copper
`Detoxification. Genes and Development 7: 1824-1835.
`
`
`28. Zhou, P., Szczypka, M. S., Young, R. and Thiele, D. J. (1994) A System for
`Gene Cloning and Manipulation in the Yeast Candida glabrata. Gene 142: 135-140.
`
`
`29. Tamai, K. T., Liu, X., Silar, P., Sosinowski, T. and Thiele, D. J.(1994) Heat Shock
`Transcription Factor Activates Yeast Metallothionein Gene Expression in Response to
`Heat and Glucose Starvation Via Distinct Signalling Pathways. Molecular and Cellular
`Biology 14: 8155-8165.
`
`30. Szczypka, M.S., Wemmie, J., Moye-Rowley, W.S. and Thiele, D.J. (1994) A Yeast
`Protein with Homology to CFTR and Multi-Drug Resistance Associated Protein (MRP1)
`Mediates Metal Resistance. J. Biol. Chem. 269: 22853-22857.
`
`31. Wemmie, J.A., Szczypka, M.S., Thiele, D.J. and Moye-Rowley, W.S. (1994) The Yeast
`AP-1 Protein Transcriptionally Activates an ATP Binding Cassette Transporter-Encoding
`Gene YCF1 J. Biol. Chem. 269: 32592-32597.
`
`32. Knight, S.A.B., Tamai, K. T., Kosman, D. J. and Thiele, D.J. (1994) Identification and
`Analysis of a Saccharomyces cerevisiae Copper Homeostasis Gene Encoding a
`Homeodomain Protein. Molecular and Cellular Biology 14: 7792-7804.
`
`33. Li, Z., Szczypka, M.S., Lu, Y., Thiele, D.J. and Rea, P.A. (1996) The Yeast Cadmium
`Factor Protein (YCF1) is a Vacuolar Glutathione-S-Conjugate Pump. J. Biol. Chem. 271:
`6509-6517.
`
`
`
`
`
`11
`
`

`
`34. Koch, K.A. and Thiele, D.J. (1996) Autoregulation by a Candida glabrata Copper
`Metalloregulatory Transcription Factor Requires Critical Minor Groove Contacts.
`Molecular and Cellular Biology 16: 724-734.
`
`35. Liu, X. and Thiele, D.J. (1996) Oxidative Stress Induces Heat Shock Factor
`Phosphorylation and HSF-Dependent Activation of Yeast Metallothionein Gene
`Transcription. Genes and Development 10: 592-603.
`
`36. Knight, S.A.B., Labbé, S., Kwon, L.F., Kosman, D.J. and Thiele, D.J. (1996) A
`Widespread Transposable Element Masks Expression of a Yeast Copper Transport
`Gene. Genes and Development 10: 1917-1929.
`
`
`37. Zhu, Z., and D.J. Thiele. (1996) A Specialized Nucleosome Modulates the Kinetics of
`Transcription Factor Binding to a Candida glabrata Metal Responsive Promoter in vivo.
`Cell 87: 459-470.
`
`
`38. Catallo, W. J., Henk, W., Younger, L., Mills, O., Thiele, D.J. and Meyers, S.P. (1996)
`Trace Metal Uptake by Pichia spartinae, an Endosymbiotic Yeast in the Salt Marsh Cord
`Grass Spartina alterniflora. Chemistry and Ecology 13:10-15 .
`
`39. Li, Z.-S., Lu, U.-P., Zhen, R.-G., Szczypka, M.S., Thiele, D. J. and Rea, P.A. (1997)A
`New Pathway for Vacuolar Cadmium Sequestration in Saccharomyces cerevisiae:
`YCF1-catalyzed transport of bis (glutathionato) cadmium. Proc. Natl. Acad. Sci., USA
`94: 42-47.
`
`40. Szczypka, M.S., Zhu, Z., Silar, P. and Thiele, D.J. (1997) Alterations in Iron and Copper
`Homeostasis in Yeast Cells Defective in Vacuolar Assembly and Acidification Yeast 13:
`1423-1435.
`
`41. Labbé, S., Zhu, Z. and Thiele, D. J. (1997) Copper-Specific Transcriptional Repression
`of Yeast Genes Encoding Critical Components in the Copper Transport Pathway.
`Journal of Biological Chemistry 272: 15951-15958.
`
`42. Koch, K.A., Peña, M. M. O. and Thiele, D.J. Copper Binding Motifs in Catalysis,
`Transport, Detoxification and Signalling. (1997) Chemistry and Biology 4: 549-560.
`
`Liu, X.-D., Liu, P. C. C., Santoro, N. and Thiele, D. J. (1997) Conservation of a Stress
`43.
`Response: Function of Human Heat Shock Transcription Factors in Yeast. EMBO J. 16:
`6466-6477.
`Zhu, Z., Labbé, S., Peña, M.M.O. and Thiele, D.J. (1998) Copper Differentially
`Regulates the Activity and Degradation of Yeast Mac1 Transcription Factor. Journal of
`Biological Chemistry 273: 1277-1280.
`
`
`44.
`
`
`
`
`
`12
`
`

`
`45. Peña, M. M. O., Koch, K. A. and Thiele, D. J. (1998) Dynamic Regulation of the
`Copper Transport and Detoxification Genes in Saccharomyces cerevisiae.
`
`Molecular and Cellular Biology 18: 2514-2523.
`
`
`46. Santoro, N., Johansson, N. and Thiele, D. J. (1998) Heat Shock Element Architecture
`Is an Important Determinant in the Temperature and Trans- Activation Domain
`Requirements for Heat Shock Transcription Factor. Molecular and Cellular Biology 18:
`6340-6352.
`
`47. Morano, K.A., Santoro, N., Koch, K.A. and Thiele, D.J. (1999) A Trans-Activation
`Domain in Yeast Heat Shock Transcription Factor is Essential for Cell Cycle
`Progression During Stress. Molecular and Cellular Biology 19: 402-411.
`Labbé, S. and Thiele, D. J. (1999) Copper Ion Inducible and Repressible Promoter
`Systems in Yeast. Methods in Enzymology 306: 145-153.
`
`
`48.
`
`
`49. Koch, K.A. and Thiele, D.J. (1999) Characterization of a Homopolymeric (dA-dT)
`Element that Confers Nucleosomal Access to Yeast and Mammalian Transcription
`Factors Journal of Biological Chemistry 274: 23752-23760.
`Liu, P.C.C. and Thiele, D.J. (1999) A Linker Domain Modulates Human Heat Shock
`Transcription Factor Trimerization in Yeast and Human Cells. Journal of Biological
`Chemistry 274: 17219-17225.
`Tanabe, M., Sasai, N., Nagata, K., Liu, X.-D., Liu, P.C.C., Thiele, D.J. and Nakai, A.
`(1999) The Human HSF4 gene generates both an activator and a repressor of the
`constitutive expression of heat shock genes by alternative splicing. Journal of Biological
`Chemistry 274: 27845-27856.
`
`52.
`Liu, X.-D., Morano, K.A. and Thiele, D. J. The yeast Hsp110 Family Member, Sse1, is
`an Hsp90 Co-chaperone. (1999) Journal of Biological Chemistry 274: 26654-26660.
`
`53. Morano, K.A. and Thiele, D.J. The Sch9 protein kinase regulates Hsp90 chaperone
`complex signal transduction activity in vivo. (1999) EMBO Journal 18: 5953-5962.
`
`54. Labbé, S., Peña, M.M.O., Fernandes, A. R. and Thiele, D.J. (1999) A Copper Sensing
`Transcription Factor Regulates Iron Uptake Genes in Schizosaccharomyces pombe.
`Journal of Biological Chemistry 274: 36252-36260.
`
`55.
`Labbé, S. and Thiele, D. J. (1999) Pipes and Wiring: The Regulation of Copper Uptake
`and Distribution in Yeast. Trends in Microbiology 7: 500-505.
`
`56. Shinyashiki, M., Chiang, K.T., Switzer, C.H., Gralla, E. B., Valentine, J.S., Thiele, D.J.
`and Fukuto, J.M. (2000) The Interaction of Nitric Oxide (NO) with the Yeast
`
`
`50.
`
`
`51.
`
`
`
`13
`
`

`
`
`59.
`
`
`62.
`
`
`63.
`
`Transcription Factor Ace1: A Model System for NO-Protein Thiol Interactions with
`Implications to Metal Metabolism. Proc. Natl. Acad. Sci., USA 97: 2491-2496.
`
`
`57. Chiang, K.T., Shinyashiki, M., Switzer, C.H., Gralla, E. B., Valentine, J.S., Thiele, D.J.
`and Fukuto, J.M. (2000) The Effects of Nitric Oxide (NO) on the Copper-Responsive
`Transcription Factor Ace1 in Saccharomyces cerevisiae: Cytotoxic and Cytoprotective
`Actions of NO. Arch. Biochem. Biophys. 377: 296-303.
`
`58. Peña, M.M.O., Puig, S. and Thiele, D.J. (2000) Functional Analysis of the
`Saccharomyces cerevisiae high affinity copper ion transporter Ctr3 Journal of Biological
`Chemistry 275: 33244-33251.
`Lee, J., Prohaska, J. R., Dagenais, S.L., Glover, T. and Thiele, D. J. (2000) Isolation of
`a Murine Copper Transporter Gene, Tissue Specific Expression and Functional
`Complementation of a Yeast Copper Transport Mutant. Gene 254: 87-96.
`
`60. Koch, K.A., Allard, S., Santoro, N., Cote, J. and Thiele, D. J. (2001) The Candida
`glabrata Amt1 copper-sensing transcription factor requires Swi/Snf and Gcn5 at a
`critical step in copper detoxification. Molecular Microbiology 40: 1-12.
`
`61. Ahn, S-G., Liu, P.C.C., Klyachko, K., Morimoto, R. and Thiele, D.J. (2001) The Loop
`Domain of Heat Shock Transcription Factor 1 Dictates DNA Binding Specificity and
`Responses to Heat Stress. Genes and Development 15: 2134-2145.
`Lee, J., Prohaska, J.R. and Thiele, D.J. (2001) Essential Role for Mammalian Copper
`Transporter Ctr1 in Copper Homeostasis and Embryonic Development. Proc. Natl.
`Acad. Sci., USA 98: 6842-6847.
`Zhou, H. and Thiele, D.J. (2001) Identification of a Novel High Affinity Copper Transport
`Complex in the Fission Yeast Schizosaccharomyes pombe. Journal of Biological
`Chemistry 276: 20529-20535.
`Liu, P.C.C. and Thiele, D.J. (2001) Novel stress responsive genes EMG1 and NOP14
`encode conserved interacting proteins required for 40S ribosome biogenesis. Molecular
`Biology of the Cell 12: 3644-3657.
`
`65.
`Lee, J., Peña, M.M.O., Nose, Y. and Thiele, D. J. (2002) Biochemical Characterization
`of the Human Copper Transporter Ctr1. Journal of Biological Chemistry 277: 4380-4387.
`
`66. Riggio, M., Lee, J., Scudiero, R., Parisi, E. , Thiele, D.J. and Filosa, S. (2002) High
`affinity copper transport protein in the lizzard Podarcis sicula. Molecular cloning,
`functional characterization and expression in somatic tissues, follicular oocytes and
`eggs. Biochem. Biophys. Acta 1576: 127-135.
`
`
`64.
`
`
`
`
`
`14
`
`

`
`67.
`
`Ishida, S., Lee, J., Thiele, D. J. and Herskowitz, I. (2002) Resistance to the anticancer
`drug cisplatin due to defects in the copper transporter Ctr1 in yeast and mammals. Proc.
`Natl. Acad. Sci. USA 99: 14298-14302.
`
`68. Puig, S. and Thiele, D.J. (2002) Molecular mechanisms of copper uptake and
`distribution. Current Opinion in Chemical Biology 6: 171-180.
`
`69. Hahn, J.-S. and Thiele, D.J. (2002) Regulation of the Saccharomyces cerevisiae Slt2
`kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. J. Biol.
`Chem. 277: 21278-21284.
`
`70. Puig, S., Lee, J., Lau, M. and Thiele, D.J. (2002) Biochemical and genetic analyses of
`yeast and human high affinity copper transporters suggest a conserved mechanism for
`copper uptake J. Biol. Chem. 277: 26021-26030.
`
`
`71.
`
`Lee, J., Petris, M. and Thiele, D.J. (2002) Characterization of Mouse Embryonic Cells
`Deficient in the Ctr1 High Affinity Copper Transporter: Identification of a Ctr1-
`independent Copper Transport System. J. Biol. Chem. 277: 40253-40259.
`
`72. Puig, S., Rees, E. M. and Thiele, D.J. (2002). The ABCDs of Periplasmic Copper
`Trafficking. Structure 10: 1292-1295.
`
`73. Petris, M.J., Smith, K., Lee, J. and Thiele, D.J. (2003) Copper stimulated endocytosis
`and degradation of the human copper transporter hCtr1. J. Biol. Chem. 278: 9639-9646.
`
`74. Ahn, S.-G. and Thiele, D. J. (2003) Direct redox regulation of mammalian Heat Shock
`Factor 1 is essential for Hsp gene activation and cellular protection from stress. Genes
`and Development 17: 516-528.
`
`75. Sancenon, V., Puig, S., Mira, H., Thiele, D.J. and Peñarrubia, L. (2003) Identification of
`a copper transporter family in Arabidopsis thaliana. Plant Molecular Biology 51: 577-
`587.
`
`76. Hahn, J.-S., Hu, Z., Iyer, V. and Thiele, D.J. (2004) Genome wide analysis of the
`biology of stress responses through heat shock transcription factor. Molecular and
`Cellular Biology 24: 5249-5256.
`Zhou, H., Cadigan, K. and Thiele, D.J. (2003) A Copper Regulated Transporter is
`Required for Copper Acquisition, Pigmentation and Development in Drosophila
`melanogaster. J. Biol. Chem. 278: 48210-48218.
`
`78. Puig, S. and Thiele, D.J. (2004) CTI6 is an Rpd3-Sin3 histone deacetylase associated
`protein required for growth under iron-limiting conditions in Saccharomyces cerevisiae.
`J. Biol. Chem. 279: 30298-30306.
`
`
`77.
`
`
`
`15
`
`

`
`
`79. Guo, Y., Smith, K., Lee, J., Thiele, D. J. and Petris, M.J. (2004) Identification of
`methionine-rich clusters that regulate copper-stimulated endocytosis of the human Ctr1
`copper transporter. J. Biol. Chem. 279: 17428-17433.
`
`80. Hahn, J.S and Thiele, D.J. (2004) Activation of the Saccharomyces cerevisiae Heat
`Shock Transcription Factor Under Glucose Starvation Conditions by Snf1 Protein
`Kinase. J. Biol. Chem. 279: 5169-5176.
`
`81. Sancenon, V., Puig, S., Mateu, I., Ynsa, M.D., Ager, F.J., Thiele, D.J. and Peñarrubia, L.
`(2004) Arabidopsis copper transporter COPT1 regulates root elongati