`
`St. Eeam “K1111 Gil gsraductiexa accmding E3
`the Beazuisiaa patent,” Natebmk mga,
`Negmme Tec11n0E<3gies & Bimegaurces
`(ZSG9)
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`1
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`1
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`AKBM 1104
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`AKBM 1104
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`‘.4‘>‘3'¢?S€
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`;'§eICS£.Q-i3f.§£ £8 iU€§~?3.£.S-S£3'<.."3?Cfi5
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`Report R 3:. £3 activities it1'Nevember{)9
`Project: New marine Ettknttrtfi-S‘ & r3e.‘}ze:' p_r'oa"tec:rs
`Made by: Pierre St-jean
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`Kriii {Bil preductitm aeeerding to the 36331630311 patent
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`Methttdttiegy
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`Extraetitm aceeraziitag to the Beatadeitt patent
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`Step 1
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`3 kg ofktiii split into ti X 5003;
`Add 6 volumes at‘ acetone
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`Grind for 28 mitt.
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`Extract for 12!} min. without agitation
`Iiiiiter
`Wash seiiti with 3 voiitmes of aeetette
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`Combine the twfl fiittates
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`Evap0ra§'.e the aceimte
`Separate the fat from the water by deeatttatieti after addititm of 1 volume of acetone
`Reezmr-ea‘ Oi}.
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`E‘vap0rat.e under vac L! 1! m
`Pitt aside
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`Step 2
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`Extract frorn svaiiti with 2 volumes of ethane! 30 min at 4"‘(I
`Fiiter
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`Combine with the fiitrate to the f"tt'..~;t oi}. extraction
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`Evaperate under vacuum
`Heat at I25‘?
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`Resttits
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`The yield ofeii protiuetion. was more than fififig, The oi? is reddish brown anti it is very otiet‘ou.s.
`Extraction of kriii in the fitztt extraction gave a stable €1'I'EI_!i:-_ii01'2
`that we hati to ‘break by adding
`acetone to teciuee the voiume of water associated with the fat.
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`2
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`Ragpofi d’activité'§R&D novmlzbrc-2 09
`Projct : N014’ veiie I3.*0ma.s'3e marzmz &. aux:-'83 prodmrs
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`Prmiusctian cfiauifie tie ieriii scion ie brevei; de Beaudnin
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`Nléihadalugie
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`Extractiois 3230:: breve‘: Beaudain
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`Etape 1
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`3 kg 01-: kriii K31 RE fractiormés en 6 X 5€}£3g
`Ajmaicr 6 voiumt-:3 <.i’a<:é1'o.nc
`B;'0yc1' pendant 20 min.
`Extraire pendant 125 min. sans agitation
`Fiitrer
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`Lava‘ Faquatéinc was 2 Voiuancs d'”acét0uc
`Combincr lcs deux fiiiira.ts
`Evaporer 1°a.cét0ne
`Eééparer ie gras tie i’eaa.1.par 1.'1éC3£1tflti0£‘! aprés ajout de 1 voiume d’acét0n-3
`Récupérrcr i"nu'E}c
`Evaporcr sous vidc
`Rése;'ve;'
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`éltape. 2
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`Extraim 1’aq_uatéine a.x.»‘ee;: 2 volumes iféthanai 30 min £1 4°C
`ii~"ii'rrer
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`jcindrc lc fiitrat is. Fhuilc cit: la prcmiérc extraction
`Evapm'c1' sous Vida
`{Ih.a:.:ft'er 5‘: 125°C
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`Résuitats
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`Ifhuiie: est do coulsur mtan-rouge ct‘
`de plus dc 360g.
`L-C i'i.3i1dCm{2‘:’1’t dc produciiura efhuiltz a
`ciic c;~;tt1'és odoraaatc. L’ext1'acticm du KI} RE. a donné en prcmiérc cxtractian unit éanuisioal stabie
`(.31,-Son a (if: iariser par ajaut d’a.cét0ne pour réd uire Ee vralum-3 d'"e-an associé an gras.
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`05 dé(:(’!?4.-|_5:‘d 2099
`3
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