`From:
`Sent:
`Subject:
`
`Hei-Mun Christina Fan[chfan@stanford.edu]
`Kael Fischer
`Mon 9/8/2008 6:21 :40 AM
`Re: quick question about solexa analysis
`
`I don't remember in that particular case, but the normal procedure (in
`our hands) is to calculate the phasing based on the phiX lane. Do you
`have the pipline run logs? That would be one place to look.
`
`Kael Fischer, Ph.D
`DeRisi Lab - Univ. Of California San Francisco
`415-514-4320
`
`On Sun, Sep 7, 2008 at 11 :04 PM, Hei-Mun Christina Fan
`<chfan@stanford.edu> wrote:
`>
`>Hi Kael,
`> Just a quick question about the analysis you helped us with back in May.
`> Were the phasing/prephasing values for each lane automatically calculated or were the values
`of the phiX control lane used for all the sample lanes?
`> We heard that tweaking these parameters would help reduce the GC bias of the sequence
`data, which we have been observing for 3 runs ... so we are curious to know what the settings
`were ...
`>Thanks for your help!!
`>Christina
`>
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`STANFORD EXHIBIT 2128
`SEQUENOM v. STANFORD
`CASE IPR2013-00390
`
`