`Sent: Thursday, April17, 2008 2:50PM
`To: Christina Fan
`Subject: Re: 454 sequencing
`That sounds fine. Good luck with the library prep.
`
`Clement
`
`Thu, Apr 17, 2008 at 1:57PM, Christina Fan <chfan@stanford.edu> wrote:
`Hi Clement,
`I obtained a library prep kit from Alex and I will prepare the libraries next week (7 total). I will let you know when I have
`them ready, but let's plan to have a run on April30. Is that ok?
`Thanks for your help!
`Christina
`
`Clement Chu wrote:
`
`: Hi Christina,
`
`: Most days are ok as I am fairly flexible. What day would you like to
`, perform the run? You can either perform cluster generation one day and
`: begin sequencing the next, or you can do both on the same day (it makes for
`: a long day).
`
`Clement
`
`' -----Original Message-----
`: From: Christina Fan [mailto:chfan@stanford.edu] Sent: Friday, April11, 2008 5:41PM
`, To: Clement Chu
`Subject: Re: 454 sequencing
`
`• Hi Clement,
`· I emailed you a few days ago about setting up the dates for solexa sequencing.
`· We would want to schedule for a run around May 1, and another one the week following.
`Joe said he has reagents in stock for us.
`: Just want to see what the dates are good for you.
`· Thanks so much!
`· Christina
`
`· Clement Chu wrote:
`
`Hi Steve and Christina,
`
`Sounds great. I can definitely get you guys on around that time frame.
`
`•In
`
`terms of reagent availability here, I would ask Joe. We don't have any
`stocked in the CAT but I know Joe and Jonathan have been buying multiple
`kits at once and are probably the best people to ask. The lead time on
`reagents direct from Illumina seems to be about 2 weeks or so.
`
`Best,
`Clement
`
`STANFORD EXHIBIT 2124
`SEQUENOM v. STANFORD
`CASE IPR2013-00390
`
`
`
`-----Original Message-----
`From: srquakeo'li(gmail.com [mailto:srquake@gmail.com] On Behalf Of Stephen
`Quake
`Sent: Friday, April 04, 2008 5:20PM
`To: Clement Chu
`Cc: Christina H. Fan
`Subject: Re: 454 sequencing
`
`ok, we have our act together now and are interested in making a full
`run on may 1, plus or minus a few days. how is the sign up, reagenet
`availablility etc looking?
`
`best,
`
`steve
`
`On Tue, Jan 29, 2008 at 1:38PM, Clement Chu <Clement.Chu@ucsf.edu> wrote:
`
`Hi Steve,
`
`Good to hear from you. Joe certainly did mention your interest. We
`
`don't
`
`or
`
`stock any reagents in the facility itself but if you need reagents on a
`quick timetable, Joe might be able to help you there. We are still
`restricting the user base so we could probably work you in within a week
`
`so of when you have a library prepared. Let me know if you need any
`more
`info or if you have a timetable in mind.
`
`Best,
`Clement
`
`-----Original Message-----
`From: srquake@gmail.com [mailto:srquake@gmail.com] On Behalf Of
`Stephen
`Quake
`Sent: Tuesday, January 29, 2008 9:49AM
`To: Clement. Chu@ucsf. edu
`Cc: Christina Fan
`Subject: Fwd: 454 sequencing
`
`Clement
`
`Joe Derisi might have told you \Ve are interested in running a few
`samples on your Solexa. How long is the queue to get something on the
`machine and do you guys stock the sequencing reagents?
`
`best,
`
`
`
`Steve
`
`---------- Fonvarded message ---------(cid:173)
`From: Joe DeRisi <joe@derisilab.ucsf.edu>
`Date: Jan 9, 2008 9:31PM
`Subject: Re: 454 sequencing
`To: Stephen Quake <quake@stanford.edu>
`
`Hi Steve,
`Great. The Solexa guy here is Clement Chu. His email is
`Clement.Chu@ucsf.edu. I will let him know that you or someone from
`your lab will contact him.
`
`Our machine is currently configured for 8 lanes/flowcell, with 330
`tiles per lane. For a good library, you should get 20-30k clusters per
`tile, which, in an ideal world, would give you about 9 million reads
`per lane, each 36nt. In reality, folks are getting 2-4 million, and
`the 8th lane is usually reserved for a control. It is clear the read
`length can be extended, if you are willing to tolerate some additional
`errors.
`Depending on your application, we can advise on library construction
`strategies.
`
`thanks for dropping by the other day- it was a big help.
`
`-Joe
`
`On Jan 9, 2008 3:06PM, Stephen Quake <quake@stanford.edu> wrote:
`
`JOe,
`
`the person in my lab to contact about a 454 run is rick
`white. each
`full run is 400,000 reads of~ 250 bp each. the
`configuration is two
`large chambers, which are independently loaded (ie can
`be different
`samples). there are other configurations (up to 16
`independent
`samples) and it is possible to do runs of shorter reads
`(1 OObp ), which
`are slightly cheaper.
`
`best
`
`steve
`
`Stephen Quake
`Professor of Bioengineering
`Stanford University
`
`PLEASE REPLY TO: quake@stanford.edu
`
`
`
`Stephen Quake
`Professor of Bioengineering
`Stanford University
`
`PLEASE REPLY TO: quake@stanford.edu
`
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