`From:
`Sent:
`Subject:
`
`'Stephen Quake'[quake@stanford.edu]; chfan[chfan@stanford.edu]
`Clement Chu
`Sat 4/5/2008 12:33:01 AM
`RE: 454 sequencing
`
`Hi Steve and Christina,
`
`Sounds great. I can definitely get you guys on around that time frame. In
`terms of reagent availability here, I would ask Joe. We don't have any
`stocked in the CAT but I know Joe and Jonathan have been buying multiple
`kits at once and are probably the best people to ask. The lead time on
`reagents direct from Ilium ina seems to be about 2 weeks or so.
`
`Best,
`Clement
`
`-----Original Message-----
`From: srquake@gmail.com [mailto:srquake@gmail.com] On Behalf Of Stephen
`Quake
`Sent: Friday, April 04, 2008 5:20 PM
`To: Clement Chu
`Cc: Christina H. Fan
`Subject: Re: 454 sequencing
`
`ok, we have our act together now and are interested in making a full
`run on may 1, plus or minus a few days. how is the sign up, reagenet
`availablility etc looking?
`
`best,
`
`steve
`
`On Tue, Jan 29, 2008 at 1 :38 PM, Clement Chu <Ciement.Chu@ucsf.edu> wrote:
`>Hi Steve,
`>
`>Good to hear from you. Joe certainly did mention your interest. We don't
`> stock any reagents in the facility itself but if you need reagents on a
`> quick timetable, Joe might be able to help you there. We are still
`> restricting the user base so we could probably work you in within a week
`or
`> so of when you have a library prepared. Let me know if you need any more
`> info or if you have a timetable in mind.
`>
`>Best,
`>Clement
`>
`>
`> -----Original Message-----
`> From: srquake@gmail.com [mailto:srquake@gmail.com] On Behalf Of Stephen
`>Quake
`> Sent: Tuesday, January 29, 2008 9:49AM
`>To: Clement.Chu@ucsf.edu
`> Cc: Christina Fan
`> Subject: Fwd: 454 sequencing
`>
`>Clement
`
`STANFORD EXHIBIT 2123
`SEQUENOM v. STANFORD
`CASE IPR2013-00390
`
`
`
`>>
`
` Joe Derisi might have told you we are interested in running a few
`> samples on your Solexa. How long is the queue to get something on the
`> machine and do you guys stock the sequencing reagents?
`
` best,
`
` Steve
`
`>>
`
`>>
`
`>>>
`
` ---------- Forwarded message ----------
`> From: Joe DeRisi <joe@derisilab.ucsf.edu>
`> Date: Jan 9, 2008 9:31 PM
`> Subject: Re: 454 sequencing
`> To: Stephen Quake <quake@stanford.edu>
`
`>>>
`
`>>
`
`>>
`
`>>>
`
` Hi Steve,
`> Great. The Solexa guy here is Clement Chu. His email is
`> Clement.Chu@ucsf.edu. I will let him know that you or someone from
`> your lab will contact him.
`
`>>
`
` Our machine is currently configured for 8 lanes/flowcell, with 330
`> tiles per lane. For a good library, you should get 20-30k clusters per
`> tile, which, in an ideal world, would give you about 9 million reads
`> per lane, each 36nt. In reality, folks are getting 2-4 million, and
`> the 8th lane is usually reserved for a control. It is clear the read
`> length can be extended, if you are willing to tolerate some additional
`> errors.
`> Depending on your application, we can advise on library construction
`> strategies.
`
` thanks for dropping by the other day - it was a big help.
`
` -joe
`
` On Jan 9, 2008 3:06 PM, Stephen Quake <quake@stanford.edu> wrote:
`> > joe,
`> >
`> > the person in my lab to contact about a 454 run is rick white. each
`> > full run is 400,000 reads of ~ 250 bp each. the configuration is two
`> > large chambers, which are independently loaded (ie can be different
`> > samples). there are other configurations (up to 16 independent
`> > samples) and it is possible to do runs of shorter reads (100bp), which
`> > are slightly cheaper.
`> >
`> > best
`> >
`> > steve
`> >
`> > --
`> > -----------------------
`> > Stephen Quake
`> > Professor of Bioengineering
`> > Stanford University
`
`
`
`> >
`> > PLEASE REPLY TO: quake@stanford.edu
`> >
`
`>>>>
`
` --
`> -----------------------
`> Stephen Quake
`> Professor of Bioengineering
`> Stanford University
`
` PLEASE REPLY TO: quake@stanford.edu
`
`>>
`
`>>
`
` No virus found in this incoming message.
`> Checked by AVG Free Edition.
`> Version: 7.5.516 / Virus Database: 269.19.15/1249 - Release Date:
`1/29/2008
`> 9:51 AM
`
`>>>
`
` No virus found in this outgoing message.
`> Checked by AVG Free Edition.
`> Version: 7.5.516 / Virus Database: 269.19.15/1249 - Release Date:
`1/29/2008
`> 9:51 AM
`
`>>>>
`
`--
`-----------------------
`Stephen Quake
`Professor of Bioengineering
`Stanford University
`
`PLEASE REPLY TO: quake@stanford.edu
`
`No virus found in this incoming message.
`Checked by AVG.
`Version: 7.5.519 / Virus Database: 269.22.5/1359 - Release Date: 4/4/2008
`8:23 AM
`
`
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