Message
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`
`Yair Blumenfeld [yairb@stanford.edu]
`12/31/2006 3:55:36 AM
`Stephen Quake [quake@stanford.edu]
`Christina Fan [chfan@stanford.edu]
`Re: DNA samples
`
`I'm not sure what gestational age the samples are from but I imagine most of
`them are between 10-24 weeks (The study compared first and second trimester
`screens).
`I don't think that they have more than one sample per patient
`though I would love to be pleasantly surprised. We should also ask them
`for an equal number of samples from patients who did not have Down syndrome
`(also from the same study) so that if the test works, we can then blind
`ourselves to the outcome and obtain sensitivity and specificity for the
`test.
`
`Quoting Stephen Quake <quake@stanford.edu>:
`
`> I think we can test proof of principle with 2mL.
`It would be nice to
`> have samples from both early and late pregnancy (not necessarily from
`> same mom) and it would be quite important to choose samples for which
`> the fetus is male (so we can do a positive control with
`> gender-specific marker).
`>
`>our first step would be to verify that we can get enrichments similar
`> to what are claimed in the literature; if we do, then the downs test
`> is a slam dunk.
`>
`> steve
`>
`>on 12/29/06, Yair Blumenfeld <yairb@stanford.edu> wrote:
`> > Hi steve,
`> >
`> > I just got word from columbia regarding the samples. They wanted us to
`> know
`>>that the samples are stored as serum and only a few cc's exist for each
`I realize that in an ideal world we would get 10 cc of serum.
`> > patient.
`Is
`>
`> > there any way we can still use the samples if we only have a "few
`> cc's"?
`> >They are not sure of the exact volume but it could be from 2-5.
`> >
`>>Yair
`> >
`> >
`>
`>
`>
`> -----------------------
`> Stephen Quake
`> Professor of Bioengineering
`> stanford University
`>
`> PLEASE REPLY TO: quake@stanford.edu
`>
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`
`STANFORD EXHIBIT 2106
`SEQUENOM v. STANFORD
`CASE IPR2013-00390
`
`

`

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