Case 1:14-cv-02758-PAC Document 61-1 Filed 05/08/15 Page 1 of 34
`
`Kowa Company, Ltd. et al. v. Aurobindo Pharma Limited et al.,
`Civil Action No. 14-CV-2497 (PAC) (and related cases)
`
`
`Exhibit 1 to Declaration of David H.
`Sherman, Ph.D., dated May 8, 2015,
`in support of Defendants’ Joint
`Opening Claim Construction Brief
`
`
`
`
`
`

`

`Case 1:14-cv-02758-PAC Document 61-1 Filed 05/08/15 Page 2 of 34
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`Curriculum Vitae
`
`
`DAVID HOWARD SHERMAN
`Address
`
`University of Michigan
`Life Sciences Institute
`210 Washtenaw Avenue
`Ann Arbor, Michigan 48109-2216
`734-615-9907 (FAX: 734-615-3641)
`Email: davidhs@umich.edu
`
`Personal Data
`
`Date of Birth: June 12, 1957 (Minneapolis, Minnesota)
`Marital Status: Married, 30 August 1981 to Carey Welles Wexler, three children (Maja, Jonathan,
`Hannah)
`Citizenship: USA
`
`Education
`
`B.A. in Chemistry (with Honors) from the University of California, Santa Cruz, CA: 1975 - 1978
`Research Advisor: Professor Phil Crews
`
`Ph.D. in Organic Chemistry from Columbia University, New York City, NY; 1978 - 1981
`Research Advisor: Professor Gilbert Stork
`
`Professional Appointments
`
`1981 - 1982
`1982 - 1984
`1984 - 1987
`1987 - 1990
`1990 - 1995
`
`
`1995 – 2000
`
`
`1996 – 1998
`
`
`1997
`1998 – 2001
`1999 – 2007
`2000 – 2003
`2000 – 2003
`
`Postdoctoral, Yale University, New Haven, CT (R.E. Handschumacher)
`Postdoctoral, Massachusetts Institute of Technology, Cambridge, MA (H.N. Eisen)
`Research Scientist, Biogen Research Corp., Cambridge, MA
`Research Scientist, John Innes Institute, Norwich, U.K.
`Assistant Professor, University of Minnesota, Department of Microbiology and
`BioTechnology Institute
`Associate Professor, University of Minnesota, Department of Microbiology and
`BioTechnology Institute
`Director, University of Minnesota, Center for Microbial Physiology and Metabolic
`Engineering
`Senior Director, ChromaXome Corporation, San Diego, CA
`(sabbatical)
`Director, Microbiology, Immunology and Cancer Biology Graduate Program
`Founder and Chief Technical Consultant, Acera Biosciences, Inc.
`Director, UMN-NIGMS Biotechnology Training Program
`Professor, University of Minnesota, Department of Microbiology and BioTechnology
`Institute
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`1
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`Case 1:14-cv-02758-PAC Document 61-1 Filed 05/08/15 Page 3 of 34
`
`2003 – 2007
`2004 – 2014
`
`
`
`J. G. Searle Professor, University of Michigan, Department of Medicinal Chemistry
`Director, Center for Chemical Genomics, Life Sciences Institute, University of
`Michigan
`Professor, University of Michigan, Department of Chemistry
`2003 – present
`Professor, University of Michigan, Department of Microbiology & Immunology
`2003 – present
`2007 – present Hans W. Vahlteich Professor, University of Michigan, Department of Medicinal
`Chemistry
`2011 – present Associate Dean for Research and Graduate Education (College of Pharmacy)
`
`Honors and Awards
`
`
`1978
`
`1978
`
`1981
`1982 - 1984
`1984 - 1986
`1990 - 1992
`1992 - 1995
`2003 - 2007
`2005-
`
`American Chemical Society Award for Excellence in Undergraduate Research
`University of California, Santa Cruz, Honors in the Major (Chemistry)
`Pegram Award for Excellence in Graduate Research, Columbia University
`National Institutes of Health Postdoctoral Fellowship
`Myron A. Bantrell Postdoctoral Research Fellowship in Molecular Biology
`Eli Lilly Life Sciences Award
`Procter & Gamble University Exploratory Research Program
`John Gideon Searle Jr. Professorship, University of Michigan
`Co-chair, University of Miami Oceans and Human Health Center External Advisory
`Committee
`Hans W. Vahlteich Professorship, University of Michigan
`Elected Fellow, American Association for the Advancement of Science
`A. C. Cope Scholar Award, American Chemical Society
`Charles Thom Award, Society for Industrial Microbiology
`American Society for Microbiology Distinguished Lecturer (2015-2017)
`
`2007-
`2008
`
`2009
`
`2009
`
`2015
`
`
`Research Experience and Interests
`Undergraduate Research (U.C. Santa Cruz): A novel GC/MS method was developed for the
`identification and characterization of polysaccharide natural products from marine alga.
`
`Graduate Research (Columbia University): A general method was developed for construction of trans-
`hydrindanone ring systems, and the total synthesis of 11-keto steroids using an intramolecular Diels-
`Alder approach.
`
`Postdoctoral Research (Yale University): Studies of the immunosuppressive drug cyclosporin A were
`performed to determine its effects on thymocyte populations in mice.
`
`Postdoctoral Research (MIT): Studies were conducted on non-H-2 class I molecules of the mouse major
`histocompatibility complex. Our work showed that specific subpopulations of T cells expressed
`structurally diverse molecules encoded in the Qa-2 locus.
`
`Biogen Research Corporation (Cambridge, MA): A molecular genetic study was conducted on insulin-
`specific, class II MHC restricted T cell receptors in mice. Further biochemical and molecular genetic
`studies were conducted on Qa-2 encoded MHC molecules.
`
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`Case 1:14-cv-02758-PAC Document 61-1 Filed 05/08/15 Page 4 of 34
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`John Innes Institute (Norwich, U.K.): A molecular genetic analysis of type II polyketide synthase
`systems was conducted to explore the identity and mechanism of construction of complex secondary
`metabolites in Streptomyces.
`
`University of Michigan (Ann Arbor, MI): Research interests include synthetic chemistry, bioorganic and
`biochemical studies of natural product biosynthetic pathways from marine and terrestrial microbes.
`Metabolic engineering and microbial genomic and proteomic technologies are being developed and
`utilized for analysis and production of novel biologically active molecules and liquid fuels.
`
`Professional Memberships
`
`1978 - Present
`1982 - Present
`1987 - Present
`2000 – Present
`
`Publications
`
`1.
`
`American Chemical Society
`American Association for the Advancement of Science
`American Society for Microbiology
`Society for Industrial Microbiology
`
`Stork, Gilbert and David H. Sherman. 1982. "Efficient de novo construction of the indanpropionic
`acid precursor of 11-keto steroids. An improved internal Diels-Alder sequence," J. Amer. Chem.
`Soc. 104:3758-3759.
`
`
`2. Kranz, David M., David H. Sherman, Michael V. Sitkovsky, Mark S. Pasternack, and Herman N.
`Eisen. 1984. "Immunoprecipitation of cell surface structures of cloned cytotoxic T lymphocytes by
`clone-specific antisera," Proc. Natl. Acad. Sci. USA 81:573-577.
`
`
`3.
`
`
`4.
`
`Sherman, David H. 1984. "Increasing sensitivity of luminescent enzyme immunoassay," Trends in
`Biotechnology 2:1-2.
`
`Sherman, David H., David M. Kranz, and Herman N. Eisen. 1984. "Expression of structurally
`diverse Qa-2 encoded molecules on the surface of cloned cytotoxic T lymphocytes," Journal of
`Experimental Medicine 160:1421-1430.
`
`
`5. Devlin, James J., Georg Widera, Andrew L. Mellor, Karen Fahrner, David H. Sherman, Elisabeth
`H. Weiss, and Richard A. Flavell. 1985. "Evolution and expression of the transplantation antigen
`gene family," Federation Proceedings 44:2736-2740.
`
`
`6.
`
`
`7.
`
`
`8.
`
`
`
`Sherman, David H., David M. Kranz, and Herman N. Eisen. 1985. "Qa-2 encoded molecules
`expressed on the surface of cloned cytotoxic T lymphocytes are structurally diverse," The Cell
`Biology of the MHC, H. Vogel and B. Pernis (eds.), Academic Press, Inc., New York.
`
`Flavell, Richard A., Hamish Allen, Linda C. Burkly, David H. Sherman, Gerald L. Waneck, and
`Georg Widera. 1986. “Molecular biology of the H-2 complex," Science 223:437-443.
`
`Sherman, David H., Paula S. Hochman, Robert Dick, Richard Tizard, K.L. Ramachandran,
`Richard A. Flavell, and Brigitte T. Huber. 1987. "A molecular analysis of antigen recognition by
`insulin specific T cell hybridomas from B6 wild type and bm12 mutant mice," Molecular and
`Cellular Biology 7:1865-1872.
`
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`Case 1:14-cv-02758-PAC Document 61-1 Filed 05/08/15 Page 5 of 34
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`9. Waneck, Gerald L., David H. Sherman, Susan Calvin, Hamish Allen, and Richard A. Flavell.
`1987. "Tissue-specific expression of a transfected Qa region gene (Q7b) encoding the Qa-2
`alloantigen," Journal of Experimental Medicine 165:1358-1370.
`
`
`10. Sherman, David H., Gerald L. Waneck, and Richard A. Flavell. 1988. "Qa-2 antigen encoded by
`Q7b transfected R1.1 cells is biochemically indistinguishable from Qa-2 expressed on the surface
`of C57B1/10 mouse spleen cells," Journal of Immunology 140:138-142.
`
`
`11. Waneck, Gerald L., David H. Sherman, Paul W. Kincade, Martin G. Low, and Richard A. Flavell.
`1988. "Molecular mapping of sites in Qa-2 required for attachment of the phosphatidylinositol
`membrane anchor," Proc. Natl. Acad. Sci. USA 85:577-581.
`
`
`12. Sherman, D.H., F. Malpartida, M.J. Bibb, H.M. Kieser, S.E. Hallam, J.A. Robinson, S. Bergh, M.
`Uhlen, T.J. 1988. "Cloning and analysis of genes for the biosynthesis of polyketide antibiotics in
`Streptomyces species," in Durand, G., Bobichon, L. and Florent, J. (eds.) Proceedings of the 8th
`International Biotechnology Symposium, Paris. Societe Francaise de Microbiology, Vol. 1, pp.
`123-137.
`
`
`13. Sherman, David H., Francisco Malpartida, Maureen J. Bibb, Helen M. Kieser, Mervyn J. Bibb, and
`David A. Hopwood. 1989. "Structure and deduced function of the granaticin-producing polyketide
`synthase gene cluster from Streptomyces violaceoruber Tu22," EMBO Journal 8:2717-2725.
`
`
`14. Hopwood, David A. and David H. Sherman. 1990. "Molecular genetics of polyketides and its
`comparison to fatty acid biosynthesis," Annual Review of Genetics 24:37-66.
`
`
`15. Hopwood, David A., David H. Sherman, Chaitan Khosla, Maureen J. Bibb, Thomas J. Simpson,
`Miguel A. Fernandez, Eduardo Martinez and Francisco Malpartida. 1990. "Hybrid pathways for
`the production of secondary metabolites," in Proceedings of the Sixth International Symposium on
`the Genetics of Industrial Microorganisms (GIM 90), Strassbourg, France .
`
`
`16. Sherman, David H., M.J. Bibb, T.J. Simpson, D. Johnson, F. Malpartida, M. Fernandez-Moreno,
`E. Martinez, C.R. Hutchinson and D.A. Hopwood. 1991. "Molecular genetic analysis reveals a
`putative bifunctional polyketide cyclase/dehydrase gene from Streptomyces coelicolor and
`Streptomyces violaceoruber, and a cyclase/O-methyltransferase from Streptomyces glaucesens,"
`Tetrahedron, 47:6029-6043.
`
`
`17. Arrowsmith, T.J., F. Malpartida, D.H. Sherman, D.A. Hopwood, A. Birch, J.A. Robinson. 1992.
`"Characterization of actI-homologous DNA encoding polyketide synthase genes from the
`monensin producer Streptomyces cinnamonensis." Mol. Gen. Genet. 234:254-264.
`
`
`18. Sherman, D.H., Eung-Soo Kim, M.J. Bibb, and D.A. Hopwood, 1992. "Functional replacement of
`polyketide synthase genes in Streptomyces coelicolor by heterologous genes from a different
`polyketide pathway," J. Bacteriol. 174:6184-6190.
`
`
`19. Malmberg, Li-Hong, Sherman, D.H., Hu, Wei-Shou, 1993. "Analysis of rate-limiting reactions in
`cephalosporin biosynthesis," Annals of the New York Academy of Sciences 665:16-26.
`
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`Case 1:14-cv-02758-PAC Document 61-1 Filed 05/08/15 Page 6 of 34
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`20. Khosla, C., McDaniel, R., Ebert-Khosla, S., Torres, R., Sherman, D.H., Bibb, M.J. and Hopwood,
`D.A., 1993. "Genetic construction and functional analysis of hybrid polyketide synthases
`containing heterologous acyl carrier protein." J. Bacteriol. 175:2197-2204.
`
`
`21. Malmberg, L.-H., Hu, W.-S. and D.H. Sherman, 1993. "Precursor flux control through targeted
`chromosomal insertion of the lysine ε-aminotransferase gene in cephamycin C biosynthesis." J.
`Bacteriol. 175:6916-6924.
`
`
`22. Hopwood, D.A., C. Khosla, D.H. Sherman, M.J. Bibb, S. Ebert-Khosla, E.-S. Kim, R. McDaniel,
`W.P. Revill, R. Torres, and T.-W. Yu. 1994. "Toward an understanding of the programming of
`aromatic polyketide synthases: a genetics-driven approach," in R.H. Baltz, G.D. Hegeman, and
`P.L. Skatrud (Eds.), Industrial Microorganisms: Basic and Applied Molecular Genetics, American
`Society for Microbiology, Washington, D.C.
`
`
`23. Kim, E.-S., D.A. Hopwood and D.H. Sherman, 1994. "Analysis of type II polyketide ß-ketoacyl
`synthase specificity in Streptomyces coelicolor A3(2) by trans complementation of actinorhodin
`synthase mutants." J. Bacteriol. 176:1801-1804.
`
`
`24. Kim, E.-S., Bibb, M.J., Butler, M.J., Hopwood, D.A., and D.H. Sherman. 1994. "Sequences of the
`oxytetracycline polyketide synthase-encoding otc genes from Streptomyces rimosus." Gene
`141:141-142 (1994)
`
`
`25. Bibb, M.J., D.H. Sherman, S. Omura and D.A. Hopwood. 1994. "Cloning, sequencing and
`deduced functions of a cluster of Streptomyces genes probably encoding for biosynthesis of the
`polyketide antibiotic frenolicin." Gene 142:31-39.
`
`
`26. August, P.A., Flickinger, M.C. and D.H. Sherman. 1994. "Cloning and analysis of a locus (mcr)
`involved in mitomycin C resistance from Streptomyces lavendulae." J. Bacteriol. 176:4448-4454.
`
`
`27. Malmberg, L.H., A. Khetan, D.H. Sherman and W.-S. Hu. 1994. “Metabolic engineering of
`in Streptomyces clavuligerus.”
` In Advances
`cephalosporin biosynthesis
`in Bioprocess
`Engineering. E. Galindo & O.T. Ramirez, Eds.: 413-416. Kluwer Pub.
`
`
`28. Kim, E.-S., Cramer, K., Shreve, A., and D.H. Sherman. 1995. "Heterologous expression of an
`engineered biosynthetic pathway: functional dissection of type II polyketide synthase components
`in Streptomyces species.” J. Bacteriol. 177:1202-1207.
`
`
`29. Crosby, J., Sherman, D.H., Bibb, M.J., Revill, W.P., Hopwood, D.A., and T.J. Simpson, 1995.
`"Polyketide synthase acyl carrier proteins from Streptomyces: Expression in Escherichia coli,
`purification and partial characterization." Biochimica et Biophysica Acta 1251:32-41.
`
`
`30. Malmberg, L.-H., Hu, W.-S., and D.H. Sherman. 1995. “Effects of enhanced lysine ε-
`aminotransferase activity on cephamycin C biosynthesis in Streptomyces clavuligerus.” Applied
`Microbiol. Biotechnol 44: 198-205.
`
`
`31. Khetan, A., L.-H. Malmberg, D.H. Sherman and W.-S. Hu. 1995. “Metabolic engineering of
`cephalosporin biosynthesis in Streptomyces clavuligerus.” Annals of the New York Academy of
`Sciences 782:17-24.
`
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`32. August, P.R., J.A. Rahn, M.C. Flickinger, and D.H. Sherman, 1996. “Inducible expression of the
`mitomycin C resistance gene product (MCRA) from Streptomyces lavendulae.” Gene 175:261 -
`267.
`
`
`33. Williams, M.D., A.M. Fieno, R.A. Grant and D.H. Sherman. 1996. “Expression and analysis of a
`bacterial poly(hydroxyalkanoate) synthase in insect cells using a baculovirus system.” Prot. Exp.
`Purif. 7:203-211.
`
`
`34. Williams, M.D., J. Rahn, and D.H. Sherman. 1996. “Production of a polyhydroxyalkanoate
`biopolymer in insect cells with a modified eucaryotic fatty acid synthase.” Appl. Environ.
`Microbiol. 62:2540-2546.
`
`
`35. Sheldon, P.J., D.A. Johnson, P.R. August, H.-w. Liu and D.H. Sherman. 1997. “Characterization
`of a mitomycin resistance determinant (mrd) from the producing organism. Streptomyces
`lavendulae.” J. Bact. 179:1796 - 1804.
`
`
`36. Johnson, D., P.R. August, C. Shackleton, H.-w. Liu and D. H. Sherman. 1997. “Microbial
`resistance to mitomycins involves a redox relay mechanism.” J. Amer. Chem. Soc. 119:2576 -
`2577.
`
`
`37. Sheldon, P.J., D. A. Johnson, P.R. August, H.-w. Liu and D. H. Sherman. 1997. “Cellular self-
`protection against the antitumor antibiotic mitomycin C in Streptomyces lavendulae.” Industrial
`Microorganisms: Basic and Applied Molecular Genetics (R. Baltz, G. Hegemann and P. Skatrud),
`Vol. 34, pp. 123 - 130.
`
`
`38. Van Pilsum, J.F., D.H. Sherman, T.V. Line, A. Bedekar and L. Ayala. 1997. “Sequence
`comparison and functional analysis of amidinotransferases from eukaryotes and prokaryotes.” In
`De Deyn, P.P., Marescau, B., Qureshi, I.A., and Mori, A. (eds.) Guanidino Compounds in Biology
`and Medicine II, London: John Libbey and Company LTD; 111 - 120.
`
`
`39. Bedeker, B., R.M. Zink, D. H. Sherman, T.V. Line, and J. Van Pilsum. 1998. “The comparative
`amino acid sequences, substrate specificities and gene or cDNA nucleotide sequences of some
`procaryotic and eucaryotic amidinotransferases: implications for evolution.” Comp. Biochem.
`Physiol. 119:677-690.
`
`
`40. Zhao, L., D. H. Sherman, and H.-w. Liu. 1998. “Biosynthesis of desosamine: molecular evidence
`suggesting ß-glucosylation as a self-resistance mechanism in methymycin/neomethymycin
`producing strain, Streptomyces venezuelae.” J. Amer. Chem. Soc. 120:9374-9375.
`
`
`41. Zhao, L., D. H. Sherman, and H.-w. Liu. 1998. “Biosynthesis of desosamine: Construction of a
`new methymycin/neomethymycin analog by deletion of a desosamine biosynthetic gene.” J. Amer.
`Chem. Soc. 120:10256-10257.
`
`
`42. Xue, Y., L. Zhao, H.-w. Liu and D. H. Sherman. 1998. “A gene cluster for macrolide antibiotic
`biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity.” Proc. Natl. Acad.
`Sci. USA 95:12111-12116.
`
`
`43. Xue, Y., D. Wilson, L. Zhao, H.-w. Liu, and D. H. Sherman. 1998. Hydroxylation of
`macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in
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`Streptomyces venezuelae. Chem. & Biol. 5:661-667.
`
`
`44. Zhao, L., N.L.S. Que, Y. Xue, D. H. Sherman, and H.-w. Liu. 1998. “Mechanistic studies of
`desosamine biosynthesis: C-4 deoxygenation precedes C-3 transamination.” J. Amer. Chem. Soc.
`120:12159-12160.
`
`
`45. Borisova, S., L. Zhao, D.H. Sherman and H.-w. Liu. 1999. Biosynthesis of desosamine:
`Construction of a new macrolide carrying a genetically designed sugar moiety. Organic Lett.
`1:133-136.
`
`
`46. Zhao, L., J. Ahlert, Y. Xue, Jon S. Thorson, David H. Sherman, and Hung-wen Liu. 1999.
`Engineering A Methymycin/Pikromycin-Calicheamicin Hybrid: Construction of Two New
`Macrolides Carrying a Designed Sugar Moiety. J. Amer. Chem. Soc. 121:9881-9882.
`
`47. Mao, Y., M. Varoglu and D.H. Sherman. 1999. “Molecular characterization of two genes (mitAB)
`required for biosynthesis of the antitumor antibiotic mitomycin C.” J. Bacteriol. 181:2199-2208.
`
`
`48. Sheldon, P.R., Y. Mao, Min He and D.H. Sherman. 1999. “Mitomycin resistance in Streptomyces
`lavendulae includes a novel drug-binding protein-dependent export system.” J. Bacteriol.
`181:2507-2512.
`
`
`49. Mao, Y., M. Varoglu and D. H. Sherman. 1999. Molecular characterization and analysis of the
`biosynthetic gene cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae
`NRRL 2564. Chem. & Biol. 6:251-263.
`
`
`50. Khetan, A., L.-H. Malmberg, Y. S. Kyung, D.H. Sherman and W.-S. Hu. 1999. “Precursor and
`cofactor as a check valve for cephamycin biosynthesis in Streptomyces clavuligerus.”
`Biotechnology Progress 15:1020-1027.
`
`
`51. Han, Lei, A. Khetan, W.-S. Hu, and D.H. Sherman. 1999. “Time-lapse microscopy reveals
`temporal and spatial expression of lysine-ε-aminotransferase gene in Streptomyces clavuligerus.”
`Mol. Microbiol. 34:878-886.
`
`
`52. Belcourt, M.F., P.G. Penketh, W.F. Hodnick, D.A. Johnson, D.H. Sherman, S. Rockwell and A.C.
`Sartorelli. 1999. “Mitomycin resistance in mammalian cells expressing the bacterial mitomycin C
`resistance protein MCRA.” Proc. Natl. Acad. Sci. USA 96:10489-10494.
`
`
`53. Xue, Y., and D.H. Sherman. 2000. Alternative modular polyketide synthase expression controls
`macrolactone structure. Nature 403:571-575.
`
`
`54. Xue, Y., D. Wilson, and D.H. Sherman. 2000. “Genetic architecture of the polyketide synthases for
`methymycin and pikromycin series macrolides.” Gene 245:203-211.
`
`
`55. He, M., M. Varoglu and D.H. Sherman. 2000. “Functional analysis of conserved amino acid
`residues in the actinorhodin β-ketoacyl-ACP-synthase.” J. Bacteriol. 182:2619-2623.
`
`
`56. Chen, S. Y. Xue, D.H. Sherman, and K.A. Reynolds. 2000. “Mechanisms of molecular recognition
`in the pikromycin polyketide synthase.” Chemistry & Biology 7:907-918.
`
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`57. Khetan, A., W.-S. Hu, and D.H. Sherman. 2000. “Temporal and spatial distribution of lysine-ε-
`aminotransferase in Streptomyces clavuligerus.” Microbiology 146:1869 – 1880.
`
`
`58. Kyung, Yung Sun, W.-S. Hu and D.H. Sherman. 2001. Analysis of temporal and spatial expression
`of the CcaR regulatory element in the cephamycin C biosynthetic pathway using green fluorescent
`protein. Mol. Microbiol. 40: 530-541.
`
`
`59. Kyung, Y. S., D. H. Sherman and W.-S. Hu. 2001. Simultaneous analysis of spatio-temporal gene
`expression for cephamycin biosynthesis in Streptomyces clavuligerus. Biotechnol. Prog. 17: 1000-
`1007.
`
`
`60. He, M., P. Sheldon and D. H. Sherman. 2001. “Characterization of a novel quinone reductase
`activity for the mitomycin C binding protein (MRD): functional switching from a drug
`activating enzyme to a drug-binding protein. Proc. Natl. Acad. Sci. USA 98:926–931.
`
`
`61. Varoglu, M., Y. Mao and D. H. Sherman. 2001. Mapping the mitomycin biosynthetic pathway
`by functional analysis of the MitM aziridine N-methyltransferase. J. Amer. Chem. Soc.
`123:6712-6713.
`
`
`62. Penketh, P. G., W. F. Hodnick, M. F. Belcourt, K. Shyam, D. H. Sherman, and A. C. Sartorelli.
`2001. Inhibition of DNA Cross-linking by Mitomycin C by Peroxidase Mediated Oxidation of
`Mitomycin C Hydroquinone. J. Biol. Chem. 276:34445-34452.
`
`
`63. Xue, Y. and D. H. Sherman. 2001. Biosynthesis and combinatorial biosynthesis of pikromycin-
`related macrolides in Streptomyces venezuelae. Metabolic Engineering 3: 15-26.
`
`
`64. Chen, S., J. B. Roberts, D. H. Sherman and K. A. Reynolds. 2001. The S. venezuelae pikAV gene
`contains a transcription unit essential for expression of enzymes involved in glycosylation of
`narbonolide and 10-deoxymethynolide. Gene 263: 255-263.
`
`
`65. Wilson, D.J., Y. Xue, K. A. Reynolds and D. H. Sherman. 2001. Characterization and analysis of
`the PikD regulatory factor in the pikromycin biosynthetic pathway of Streptomyces venezuelae. J.
`Bacteriol. 183:3468-3475.
`
`
`66. Sang-Jung Kim, Han-Young Kang, and David H. Sherman. 2001. Synthesis of triketide delta-
`lactones. Synthesis 12:1790-1793.
`
`
`67. Zhang, Q. and D. H. Sherman. 2001. Isolation and structure determination of novamethymycin, a
`new bioactive metabolite of the methymycin biosynthetic pathway in Streptomyces venezuelae. J.
`Nat. Prod. 64:1447-1450.
`
`
`68. Baumann, R. P., W. F. Hodnick, H. A. Seow, M. F. Belcourt, S. Rockwell, D. H. Sherman and A.
`C. Sartorelli. 2001. Reversal of mitomycin C resistance by overexpression of bioreductive
`enzymes in Chinese hamster ovary cells. Cancer Research 61:7770-7776.
`
`
`69. Baumann, R.P., D. H. Sherman and A. C. Sartorelli. 2002. A novel selection marker for
`mammalian cell transfection. BioTechniques 32:1030-1036.
`
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`
`70. Yoon, Y.J., Beck, B. J., Kim, B.S., Kang, H.-Y., Reynolds, K. A. and David H. Sherman. 2002.
`Generation of multiple bioactive macrolides by hybrid polyketide synthases in Streptomyces
`venezuelae. Chem. Biol. 9:203-214.
`
`
`71. Beck, B. J., Yoon, Y.J., Reynolds, K.A. and David H. Sherman. 2002. The hidden steps of domain
`skipping: mechanistic studies of macrolactone ring size determination in the pikromycin modular
`polyketide synthase. Chem. Biol. 9:575-583.
`
`
`72. Kim, B.S., T. A. Cropp, G. Florova, Y. Lindsay, D. H. Sherman, and K. A. Reynolds. 2002. An
`unexpected interaction between the modular polyketide synthases, erythromycin DEBS1 and
`pikromycin PikAIV, leads to efficient triketide lactone synthesis. Biochemistry 41:10827-10833.
`
`
`73. Cropp, T.A., B.S. Kim, B.J. Beck, Y.J. Yoon, D.H. Sherman and K.A. Reynolds. 2002. Recent
`developments in the production of novel polyketides by combinatorial biosynthesis. In
`Biotechnology and Genetic Engineering Reviews Vol. 19, pp. 159-172. Intercept Ltd. Andover,
`UK
`
`
`74. Chang, Z., P. Flatt, W. H. Gerwick, V.-A. Nguyen, C. L. Willis and D. H. Sherman. 2002. The
`barbamide biosynthetic gene cluster: a novel marine cyanobacterial system of mixed PKS-NRPS
`origin involving an unusual trichloroleucyl starter unit. Gene 296:235-247.
`
`
`75. Kim, B. S., T. A. Cropp, B.J. Beck, D.H. Sherman and K.A. Reynolds. 2002. Biochemical
`evidence for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin. J.
`Biol. Chem. 277:48028-48034.
`
`
`76. Martin TW, Dauter Z, Devedjiev Y, Sheffield P, Jelen F, He M, Sherman DH, Otlewski J,
`Derewenda ZS, Derewenda U. 2002. Molecular basis of mitomycin C resistance in Streptomyces:
`structure and function of the MRD protein. Structure (Camb). 2002 10(7):933-42.
`
`
`77. Sherman, D.H. 2002. New enzymes for “warheads”. Nature Biotechnol. 20: 984-985.
`
`78. Podust, L.M., Kim, Y., Arase, M., Neely, B.A., Beck, B.B., Bach, H., Sherman, D.H., Lamb, D.C.,
`Kelly, S.C., and Waterman, M.R. 2003. The 1.92A structure of the Streptomyces coelicolor A3(2)
`CYP154C1: A new monooxygenase that functionalizes macrolide ring systems. J. Biol. Chem.
`278: 12214-12221.
`
`
`79. Beck, B.J., Aldrich, C.C., Fecik, R.A., Reynolds, K.A. and D. H. Sherman. 2003. Iterative chain
`elongation by a pikromycin monomodular polyketide synthase. J. Amer. Chem. Soc. 125:4682-
`4683.
`
`
`80. Beck, B.J., Aldrich, C.C., Fecik, R.A., Reynolds, K.A. and D. H. Sherman. 2003. Substrate
`recognition and channeling activities of mono-modules from the pikromycin polyketide synthase.
`J. Amer. Chem. Soc. 125:12551-12557.
`
`
`81. Podust L. M., Bach, H., Kim, Y., Lamb, D. C, Arase, M., Sherman, D.H., Kelly, S.L., and M. R.
`Waterman. 2004. Comparison of the 1.85 A structure of CYP154A1 from Streptomyces coelicolor
`A3(2) with the closely related CYP154C1 and CYPs from antibiotic biosynthetic pathways.
`Protein Sci. 2004. 13:255-268.
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`82. Salomon, C. E., N. A. Magarvey and D. H. Sherman. 2004. Merging the potential of microbial
`genetics with biological and chemical diversity: An even brighter future for marine natural product
`drug discovery. Natural Product Reports 21:105-121.
`
`
`83. Kim, B.-S., Sherman, D. H. and K. A. Reynolds. 2004. An efficient method for creation and
`functional analysis of libraries of hybrid type I polyketide synthases. Prot. Eng. Des. Sel. 17:277-
`284.
`
`
`84. Srinivasan, A., Bach, H., Sherman, D. H. and J. S. Dordick. 2004. Bacterial P450-catalyzed
`polyketide hydroxylation on a microfluidic platform. Biotechnol. Bioeng. 88(4):528-535.
`
`
`85. Chang, Z., Sitachitta, N., Rossi, J.V., Roberts, M.A., Flatt, P.M., Jia, J., Sherman, D.H., W. H.
`Gerwick. 2004. Biosynthetic pathway and gene cluster analysis of curacin A, an anti-tubulin
`natural product from the tropical marine cyanobacterium Lyngbya majuscula. J. Nat. Prod.
`67:1356-1367.
`
`
`86. Magarvey, N.A., Keller J.M., Bernan, V., Dworkin, M., and David H. Sherman. 2004. Isolation
`and characterization of novel marine-derived actinomycete taxa rich in secondary metabolites.
`Appl. Env. Microbiol. 70(12):7520-7529.
`
`
`87. Hildebrand, M., Waggoner, L.E., Liu, H., Sudek, S., Allen, S., Anderson, C., Sherman, D.H., and
`M. Haygood. 2004. bryA: an unusual modular polyketide synthase gene from the uncultivated
`bacterial symbiont of the marine bryozoan Bugula neritina. Chem. Biol. 11(11):1543-1552.
`
`
`88. Jeong, J.-C., Srinivasan, A., Grüschow, S., Bach, H., Sherman, D.H., and J.S. Dordick. 2005.
`Exploiting the reaction flexibility of a type III polyketide synthase through in vitro pathway
`manipulation. J. Amer. Chem. Soc. 127(1):64-65.
`
`
`89. Nishizawa, T., Aldrich, C.C. and D. H. Sherman. 2005. Molecular analysis of the rebeccamycin L-
`amino acid oxidase from Lechevalieria aerocolonigenes ATCC 39243. J. Bacteriol. 187:2084-
`2092.
`
`
`90. Fortman J.L., Sherman D. H. 2005. Utilizing the power of microbial genetics to bridge the gap
`between the promise and the application of marine natural products. ChemBioChem. 6:960-978.
`
`
`91. Aldrich, C. C., Beck, B. J., Fecik, R.A. and D. H. Sherman. 2005. Biochemical investigation of
`pikromycin biosynthesis employing native penta- and hexaketide chain elongation intermediates.
`J. Amer. Chem. Soc. 127: 8441-8452.
`
`
`92. Aldrich, C. C., Venkatraman L., Sherman, D. H. and R. A. Fecik. 2005. Chemoenzymatic
`synthesis of the polyketide macrolactone 10-deoxymethynolide. J. Amer. Chem. Soc. 127:8910-
`8911.
`
`
`93. Venkatraman L., Aldrich, C. C., Sherman, D. H. and R. A. Fecik. 2005. Formal total synthesis of
`the polyketide macrolactone narbonolide. J. Org. Chem. 70:7267-7272. [Designated one of
`20 Most-Accessed Articles during July-September 2005]
`
`
`94. Lee, S. K., Basnet, D. B., Hong, J. S. J., Jung, W. S., Choi, C. Y., Lee, H. C., Sohng, J. K., Ryu, K.
`G., Kim, D. J., Ahn, J. S., Kim, B. S., Oh, H. C., Sherman, D. H., Yoon Y. J. 2005. Structural
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`diversification of macrolactones by substrate-flexible cytochrome P450 monooxygenases. Adv.
`Synth. Catal. 347:1369-1378.
`
`
`95. Sherman, D. H. 2005. The lego-ization of polyketide biosynthesis. Nature Biotechnol. 23:1083-
`1084.
`
`96. Beck, Z.Q., Aldrich, C.C., Magarvey, N.A, Georg, G.I. and D.H. Sherman. 2005.
`Chemoenzymatic synthesis of cryptophycin/arenastatin natural products. Biochemistry 44:13457-
`13466. [Designated “Hot” article for Biochemistry, November 2005]
`
`
`97. Mehra S., Lian W., Jayapal K.P., Charaniya S.P., Sherman D.H., Hu W.S. 2006. A framework to
`analyze multiple time series data: A case study with Streptomyces coelicolor. J Ind Microbiol
`Biotechnol 33(2): 159-172.
`
`
`98. Beck, Z.Q. and Sherman, D.H. 2006. Development of an internally quenched fluorogenic
`substrate for analysis of thioesterases. Anal. Biochem. 349(2):309-311.
`
`
`99. Nishizawa, T., Grüschow, S., Jayamaha, D.E., Nishizawa-Harada, C. and Sherman, D.H. 2006.
`Enzymatic assembly of the bis-indole core of rebeccamycin. J. Amer. Chem. Soc. 128(3):724-725.
`
`
`100. Jung WS, Lee SK, Hong JS, Park SR, Jeong SJ, Han AR, Sohng JK, Kim BG, Choi CY, Sherman
`DH, Yoon YJ. 2006. Heterologous expression of tylosin polyketide synthase and production of a
`hybrid bioactive macrolide in Streptomyces venezuelae. Appl. Microbiol. Biotechnol. 72(4):763-
`769.
`
`
`101. Lee SK, Park JW, Kim JW, Jung WS, Park SR, Choi CY, Kim ES, Kim BS, Ahn JS, Sherman
`DH, Yoon YJ. 2006. Neopikromycin and novapikromycin from the pikromycin biosynthetic
`pathway of Streptomyces venezuelae. J. Nat. Prod. 69(5):847-849.
`
`
`102. Flatt PM, O'Connell SJ, McPhail KL, Zeller G, Willis CL, Sherman DH, Gerwick WH. 2006.
`Characterization of the initial enzymatic steps of barbamide biosynthesis. J. Nat. Prod. 69:938-
`944.
`
`
`103. Gu L., Jia J., Liu H., Håkansson K., Gerwick W. H., and Sherman, D. H. 2006. Metabolic
`coupling of dehydration and decarboxylation in the curacin A pathway: functional identification
`of a mechanistically diverse enzyme pair. J. Am. Chem. Soc. 128:9014-9015.
`
`
`104. Sherman D. H., Li S., Yermalitskaya L.V., Kim Y., Smith J.A., Waterman M.R., and Podust L.M.
`2006. The structural basis for substrate anchoring, active site selectivity, and product formation
`by P450 PikC from Streptomyces venezuelae. J. Biol. Chem. 281:26289-26297.
`
`
`105. Ku B., Cha J., Srinivasan A., Kwon S.J., Jeong J.C., Sherman D.H., and Dordick J.S. 2006. Chip-
`based polyketide biosynthesis and functionalization. Biotechnol. Prog. 22:1102-1107.
`
`
`106. Giraldes J.W., Akey D.L., Kittendorf J.D., Sherman D.H., Smith J.L., and Fecik R.A. 2006.
`Structural and mechanistic insights of polyketide macrolactonization from polyketide-based
`affinity labels. Nature Chemical Biology. 2:531-536.
`
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`107. Akey D.L., Kittendorf J.D., Giraldes J.W., Fecik R.A., Sherman D.H., and Smith J.L. 2006.
`Structural basis for macrolactonization by the pikromycin thioesterase. Nature Chemical Biology.
`2:537-542.
`
`
`108. Sherman D.H. and Smith J.L. 2006. Clearing the skies over modular polyketide synthases. ACS
`Chem. Biol. 1:505-509.
`
`
`109. Kittendorf J.D. and Sherman D.H. 2006. Developing tools for engineering hybrid polyketide
`synthetic pathways. Curr. Opin. Biotechnol. 17:597-605.
`
`
`110. Venkatraman L., Salomon C.E., Sherman D.H., and Fecik R.A. 2006. Total synthesis of
`narbonolide and biotransformation to pikromycin. J. Org. Chem 71:9853-9856.
`
`
`111. Magarvey N.A., Beck Z.Q., Golakoti T., Ding Y., Huber U., Hemscheidt T.K., Abelson D.,
`Moore R.E., Sherman D.H. 2006. Biosynthetic characterization