Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 1 of 188 PageID #: 13493
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 1 of 188 PageID #: 13493
`
`
`
`
`
`
`
`
`
`EXHIBIT 148
`EXHIBIT 148
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 1 of 188 PageID #: 13493
`
`
`
`
`
`
`
`
`
`EXHIBIT 148
`EXHIBIT 148
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 2 of 188 PageID #: 13494
`
`
`
`IN THE UNITED STATES DISTRICT COURT
`FOR THE DISTRICT OF DELAWARE
`
`
`
`
`
`
`C.A. No. 20-CV-1580 (LPS)
`
`
`
`) ) ) ) ) ) ) ) )
`
`
`
`GUARDANT HEALTH, INC.,
`
`
`Plaintiff,
`
`
`
`v.
`
`
`FOUNDATION MEDICINE, INC.,
`
`
`Defendant.
`
`
`
`DECLARATION OF JEREMY A. TIGAN IN SUPPORT OF
`FOUNDATION MEDICINE INC.’S SUR-REPLY IN OPPOSITION TO GUARDANT
`HEALTH, INC.’S MOTION FOR A PRELIMINARY INJUNCTION
`
`I, Jeremy A. Tigan, hereby declare:
`
`1.
`
`I am a member of the bar of the State of Delaware and an attorney at the law firm
`
`of Morris, Nichols, Arsht & Tunnell LLP, counsel to Defendant Foundation Medicine, Inc.
`
`(“Foundation Medicine”). I submit this declaration in support of Foundation Medicine’s Sur-
`
`Reply in Opposition to Guardant Health, Inc.’s (“Guardant’s”) Motion for a Preliminary
`
`Injunction.
`
`2.
`
`To the best of my knowledge and belief, the following appended documents,
`
`Appendix Exhibits 149-152 at A2706-A2887, are true and correct copies of the documents
`
`described in Foundation Medicine’s Appendix in Support of its Sur-Reply in Opposition to
`
`Guardant’s Motion for a Preliminary Injunction.
`
`I declare under penalty of perjury that the foregoing is true and correct.
`
`
`
`Dated: May 4, 2021
`
`
`
`
`
`
`
`
`
`
`
`
`
`
`/s/ Jeremy A. Tigan
`Jeremy A. Tigan (#5239)
`
`
`
`A2705
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 3 of 188 PageID #: 13495
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 3 of 188 PageID #: 13495
`
`
`
`
`
`
`
`
`
`EXHIBIT 149
`EXHIBIT 149
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 4 of 188 PageID #: 13496
`
`In the Matter Of:
`GUARDANT HEALTH vs
`
`FOUNDATION MEDICINE
`
`STACEY GABRIEL, PH.D.
`March 11, 2021
`
`A2706
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 5 of 188 PageID #: 13497
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`1
`
`· · · ·IN THE UNITED STATES DISTRICT COURT
`
`· · · · · FOR THE DISTRICT OF DELAWARE
`
`· · · · · · · · · NO.· ·20-1580
`
`
`
`_________________________________________________
`
`· · · _
`
`
`
`ARDANT HEALTH, INC.,GU
`
`· v
`
`FO
`
`NDATION MEDICINE, INC.,
`
`·
`
`· · · Defendant.
`
`
`
`__________________________________________________
`
`· · · · · · · ·VIDEO DEPOSITION OF
`
`· · · · · · · STACEY GABRIEL, PH.D.
`
`· · · · · · · ·Conducted Remotely
`
`· · · · · · Thursday, March 11, 2021
`
`· · · · · · · · · 9:03 a.m. EST
`
`
`
`_________________________________________________
`
`· · · · · _
`
`
`
`ported by:· Elisabeth A. Lorenz:· RMR, CRRRe
`
`
`
`b No. 2021-783110Jo
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 1
`
`· · · Plaintiff,
`
`. U
`
`s
`
`A2707
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 6 of 188 PageID #: 13498
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`2
`
`· · · · · · ·VIDEO DEPOSITION of STACEY GABRIEL,
`
`PH.D., conducted remotely via Zoom:
`
`· · · · · · ·Pursuant to notice, before Elisabeth A.
`
`Lorenz, Registered Merit Reporter and Certified
`
`Realtime Reporter.
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 2
`
`A2708
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 7 of 188 PageID #: 13499
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`3
`
`·1
`
`·2
`
`·3
`
`·4
`
`· · · · · · · · A P P E A R A N C E S
`
`· · · · · · · (All appearing remotely)
`
`ON BEHALF OF PLAINTIFF:
`
`·5
`· ·
`·6
`· ·
`·7
`· ·
`·8
`· ·
`·9
`
`· · ·JUSTIN CONSTANT
`· · ·DEREK WALTER
`· · ·Weil, Gotshal & Manges
`· · ·700 Louisiana
`· · ·Suite 1700
`· · ·Houston, Texas 77002-2784
`· · ·713.546.5217
`· · ·justin.constant@weil.com
`· · ·derek.walter@weil.com
`
`ON BEHALF OF DEFENDANT:
`
`· · ·ERIC MARANDETT
`· · ·STEPHANIE SCHONEWALD
`· · ·DANIEL BRUTTI
`· · ·SOPHIE WANG
`· · ·Choate, Hall & Stewart
`· · ·Two International Place
`· · ·Boston, Massachusetts 02110
`· · ·617.248.4769
`· · ·emarandett@choate.com
`· · ·sschonewald@choate.com
`· · ·dbrutti@choate.com
`· · ·swang@choate.com
`
`ALSO PRESENT:
`
`· ·Matt Downing, Videographer
`
`10
`
`11
`· ·
`12
`· ·
`13
`· ·
`14
`· ·
`15
`· ·
`16
`· ·
`17
`· ·
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 3
`
`A2709
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 8 of 188 PageID #: 13500
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`14
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`BY MR. CONSTANT:
`
`Q· · · ·And going to the previous reports and
`
`declarations that you have submitted, is there
`
`anything that you would change about those reports
`
`or declarations?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Not that I can think of
`
`right now.
`
`BY MR. CONSTANT:
`
`Q· · · ·Now, in your declaration in a few places,
`
`you refer to a standard library preparation, cfDNA.
`
`And I can point you to a few of the spots.
`
`· · · · But could you describe for me, what is the
`
`standard library preparation of cfDNA in 2013?
`
`A· · · ·Could you point me to one of the spots,
`
`please?
`
`Q· · · ·Sure.· If you look at -- it's Footnote 51 on
`
`Page 51.
`
`A· · · ·Okay.· I'm there.
`
`Q· · · ·So what is standard library preparation of
`
`cfDNA?
`
`A· · · ·So a standard library preparation would
`
`involve manipulations of the DNA in order to prepare
`
`it for DNA sequencing.
`
`Q· · · ·And in that context, what did you mean by
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 14
`
`YVer1f
`
`A2710
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 9 of 188 PageID #: 13501
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`15
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`standard?
`
`A· · · ·I think, as is pointed to in the documents,
`
`there were standard library prep -- protocols
`
`available, for example, from Illumina at the time
`
`and others -- and other vendors as well.
`
`Q· · · ·Is there a particular -- and just as an
`
`example, what would be the standard molar excess
`
`used for adaptors in standard library preparation of
`
`cfDNA?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Well, I think there's a
`
`range of possible molar -- the molar concentration
`
`of adaptors, and I discuss some of those well-known
`
`ranges here in the declaration.
`
`BY MR. CONSTANT:
`
`Q· · · ·And what would those ranges be?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Well, we can go to --
`
`I'll find the part here in which we discussed it.
`
`I'm sorry, I'm trying to -- so there are protocols
`
`which we've -- which we can look at here which have
`
`40-fold molar excess, 100-fold molar excess and so
`
`on, so there's a range.· But there's clearly a range
`
`of molar excess given.· There's -- we've -- there's
`
`also a textbook that we point to here, which we talk
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 15
`
`YVer1f
`
`A2711
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 10 of 188 PageID #: 13502
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`16
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`about -- which discusses how much molar excess
`
`should be used -- or can be used in a reaction.
`
`BY MR. CONSTANT:
`
`Q· · · ·And which paragraph are you looking at?
`
`A· · · ·I'm sorry, I've scrolled off it already.
`
`· · · · All right.· So I was looking at
`
`Paragraph 141 discussing the molar excess that --
`
`that we -- that we conceive from Schmitt.· Schmitt
`
`teaches using more than a 30-fold molar excess, in
`
`fact, more than a 40-fold molar excess, which is
`
`discussed here in Paragraph 141.
`
`Q· · · ·And what is -- and you referred to a range.
`
`· · · · But what is the range of molar excess that's
`
`used in the standard library preparation methods, as
`
`referred to in your declaration?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Well, I think that
`
`there's a range, and that range can be -- would be
`
`particular for the -- for the particular work of
`
`that experiment, so I don't think I can -- I can
`
`cite for you a single number.· You're asking for a
`
`range.
`
`· · · · · · ·I think we know that Schmitt uses at
`
`least 30-fold, and then there are -- there's a range
`
`that could be modified depending on the needs of
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 16
`
`YVer1f
`
`A2712
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 11 of 188 PageID #: 13503
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`17
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`that experiment.
`
`BY MR. CONSTANT:
`
`Q· · · ·And in -- how would the needs of the
`
`experiment affect what the molar excess would be in
`
`the context of standard library preparation?
`
`A· · · ·So I don't -- I can't give you a full range
`
`of what those needs might be.· But it's clear that
`
`there are needs and -- in certain settings to adapt
`
`with a -- with efficiency to be able to capture
`
`molecules for sequencing.
`
`· · · · And I think the use of at least a standard
`
`molar excess of something like 30-fold would be
`
`completely routine and expected, and that's what we
`
`cite here.
`
`Q· · · ·And just to be clear, it's -- I just want to
`
`make sure I understand what you -- you said before
`
`was that there is a range, and it's modified based
`
`on the needs of the experiment.
`
`· · · · So can you identify any needs of the
`
`experiment that would result in a modification of
`
`the range in the context of standard library
`
`preparation?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· So I think a need of an
`
`experiment is to essentially make available as many
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 17
`
`YVer1f
`
`A2713
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 12 of 188 PageID #: 13504
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`18
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`molecules as possible for downstream analysis and
`
`that one would look to the set of conditions in
`
`library preparation to be able to modify or optimize
`
`that as the need --
`
`BY MR. CONSTANT:
`
`Q· · · ·What kind of conditions -- I'm sorry. I
`
`apologize.
`
`· · · · What kind of conditions?
`
`A· · · ·The conditions of the library preparation
`
`reaction, such as the reagents used, the time and
`
`temperature of incubation, the molar concentration
`
`of adaptors, and so on.
`
`Q· · · ·Any other you can think of?
`
`A· · · ·I'm sure there are others, but those are the
`
`ones that come to mind right now.
`
`Q· · · ·Would the enzyme used be one of them?
`
`A· · · ·Yes.
`
`Q· · · ·The concentration of DNA?
`
`A· · · ·Yes.
`
`Q· · · ·The buffer composition?
`
`A· · · ·Yes.
`
`Q· · · ·And you referred to time.
`
`· · · · Were you just referring to the -- there --
`
`the duration of the reaction?
`
`A· · · ·Yes.
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 18
`
`YVer1f
`
`A2714
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 13 of 188 PageID #: 13505
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`19
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`Q· · · ·Are there any other factors?
`
`A· · · ·Earlier I mentioned the temperature.· Like I
`
`said, there's probably others that we're not naming,
`
`but those are the ones that are -- that I can think
`
`of right now.
`
`Q· · · ·How would you distinguish between standard
`
`and nonstandard library preparation?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· I don't think I have a
`
`single definition to give you.· I can't recall if I
`
`discussed a nonstandard library preparation in my
`
`declaration.· If I did, you could point me to it.
`
`BY MR. CONSTANT:
`
`Q· · · ·In your mind, what is -- what does it mean
`
`to be a standard library preparation versus a
`
`nonstandard?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Well, there's basic
`
`components to library preparation, some of which you
`
`and I just talked about.· So I would say using those
`
`is standard.· And there are ways to optimize or
`
`change features of the reaction, some might consider
`
`nonstandard, others might not.
`
`· · · · · · ·But library preparation was, you know,
`
`well known at the time of -- of the time in question
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 19
`
`YVer1f
`
`A2715
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 14 of 188 PageID #: 13506
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`30
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`that.
`
`BY MR. CONSTANT:
`
`Q· · · ·Did you talk to anybody about running any
`
`experiments for the purpose of your declaration?
`
`A· · · ·No.
`
`Q· · · ·Did you ask anybody if you could perform any
`
`experiments for your declaration?
`
`A· · · ·No.
`
`Q· · · ·And why not?
`
`A· · · ·I didn't need to, and I wasn't asked to.
`
`Q· · · ·And what do you mean by you -- you didn't
`
`need to run any experiments?
`
`A· · · ·Well, the analysis I performed was directed
`
`to whether a person of skill in the art would have
`
`known how to increase ligation efficiency in 2014.
`
`So I don't believe experimentation by me is
`
`necessary to perform that analysis.
`
`Q· · · ·Is there any way to know what the ligation
`
`efficiency in a specific set of conditions would be
`
`without running the experiment?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Well, one can look at a
`
`product's notes insert that might come with a kit
`
`that might tell you a range to expect.
`
`· · · · · · ·But if you're talking about your own
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 30
`
`YVer1f
`
`A2716
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 15 of 188 PageID #: 13507
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`35
`
`Q· · · ·I'd like to turn your attention to
`
`Paragraph 147 regarding
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 35
`
`YVer1f
`
`A2717
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 16 of 188 PageID #: 13508
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`38
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 38
`
`YVer1f
`
`A2718
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 17 of 188 PageID #: 13509
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`39
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 39
`
`YVer1f
`
`A2719
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 18 of 188 PageID #: 13510
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`45
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· It's -- sorry.· I don't
`
`know.
`
`BY MR. CONSTANT:
`
`Q· · · ·Can you describe the -- the magnitude of the
`
`difference caused by -- or the magnitude of --
`
`strike that.
`
`· · · · Can you describe the magnitude of the change
`
`of the ligation efficiency in response to this
`
`temperature change?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· I cannot.
`
`· · · · · · ·MR. CONSTANT:· I think it's a good time
`
`for a quick five-minute break if that works for you.
`
`· · · · · · ·MR. MARANDETT:· Sure.
`
`· · · · · · ·THE VIDEOGRAPHER:· All right.· The time
`
`is 10:07 a.m.· We are off the record.
`
`· · · · · · ·(Recess:· 10:07 a.m. to 10:16 a.m.)
`
`· · · · · · ·THE VIDEOGRAPHER:· The time is
`
`10:16 a.m.· We are back on the record.
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 45
`
`YVer1f
`
`A2720
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 19 of 188 PageID #: 13511
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`46
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`BY MR. CONSTANT:
`
`Q· · · ·What is the basis of that expectation?
`
`A· · · ·The changes I feel are relatively small
`
`changes.
`
`Q· · · ·And you believe that change is minor?
`
`A· · · ·I think given that it's one component of a
`
`complex reaction, I would say -- you know, a
`
`reaction with many components, I would say it's --
`
`it's not a huge change in my opinion.
`
`Q· · · ·And what is your understand -- and you can't
`
`say, one way or the other, whether or not it would
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 46
`
`YVer1f
`
`A2721
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 20 of 188 PageID #: 13512
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`47
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`change the ligation efficiency up or down?
`
`A· · · ·I don't know specifically, but it's an
`
`overall similar -- very similar experiment and
`
`similar experimental conditions.
`
`Q· · · ·Can you say, one way or the other, whether
`
`or not the change in the dTTP concentration would
`
`change the ligation efficiency up or down?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· No, I cannot.
`
`BY MR. CONSTANT:
`
`Q· · · ·And can you describe the magnitude of the
`
`change to the ligation efficiency caused by the
`
`change in the concentration of the dTTP -- dTTP?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Not precisely.· But as I
`
`said, given the -- the methods are so similar, I
`
`would not expect a big change, but I can't tell you
`
`an exact number.
`
`BY MR. CONSTANT:
`
`Q· · · ·Could it cause a big change?
`
`A· · · ·Could it cause?· Unlikely to.
`
`Q· · · ·And why do you think it's unlikely?
`
`A· · · ·Again, I just think that these are overall
`
`very similar experimental conditions.
`
`Q· · · ·And do you have any basis for anything you
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 47
`
`YVer1f
`
`A2722
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 21 of 188 PageID #: 13513
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`48
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`can point me to -- strike that.
`
`· · · ·
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· It's based on my
`
`knowledge as someone who's been in this field for
`
`25 years.
`
`BY MR. CONSTANT:
`
`Q· · · ·Can you point to anything specific?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· No, I -- nothing
`
`specific.· It's based on my knowledge.
`
`BY MR. CONSTANT:
`
`Q· · · ·Other than performing the experiment, is
`
`there anything that you would look to to determine
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 48
`
`YVer1f
`
`A2723
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 22 of 188 PageID #: 13514
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`49
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`the answer to that question?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· I could look at published
`
`protocols that use the same or similar reaction
`
`conditions and see what their results were.· That
`
`could influence my expectation.
`
`BY MR. CONSTANT:
`
`Q· · · ·But you haven't done that; is that correct?
`
`A· · · ·I mean, I have pointed to other references
`
`that indicate that receiving the benefit --
`
`achieving greater than 20 percent ligation
`
`efficiency was -- was possible and standard at the
`
`time.· So I guess the fact that these changes aren't
`
`big doesn't surprise me, then, that there -- I would
`
`expect, then, I would say, that achieving at least
`
`20 percent was doable
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`
`
`888-267-1200 Page 49
`
`YVer1f
`
`A2724
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 23 of 188 PageID #: 13515
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`51
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· That's correct.· I would
`
`need to know the particulars of the experiment.
`
`BY MR. CONSTANT:
`
`Q· · · ·Turning to the difference of the
`
`temperature -- actually, let's just -- sorry, going
`
`back.
`
`· · · · Looking at the Schmitt example method, if
`
`you were to increase the adaptor concentration by
`
`tenfold, what would be your expectation of the
`
`change of the ligation efficiency?
`
`A· · · ·I don't know.· I have not studied that. I
`
`don't know.
`
`Q· · · ·Have you studied any particular experiment
`
`as to the effect of ligation -- or strike that.
`
`· · · · Have you studied any particular experiment
`
`as to the effect of adaptor concentration on
`
`ligation efficiency?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Not that I can recall,
`
`no.
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 51
`
`YVer1f
`
`A2725
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 24 of 188 PageID #: 13516
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`52
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· The difference in
`
`temperature is relatively small, so I wouldn't
`
`expect much of a difference, but I can't give you an
`
`exact number.
`
`BY MR. CONSTANT:
`
`Q· · · ·And you can't tell me, one way or the other,
`
`whether or not it would increase or decrease the
`
`ligation efficiency?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· No, I don't know.
`
`BY MR. CONSTANT:
`
`Q· · · ·And when you say that you would expect it to
`
`be small, what do you mean by small?
`
`A· · · ·Actually, I said the temperature difference
`
`was small, so I wouldn't expect a huge difference --
`
`Q· · · ·Well, when you say --
`
`A· · · ·-- in that.
`
`Q· · · ·-- yeah, you wouldn't expect a huge
`
`difference, what do you mean by huge difference?
`
`A· · · ·Again, I don't have an exact number for you.
`
`But the temperature difference is small, so I would
`
`expect similar performance of these two reactions.
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 52
`
`YVer1f
`
`A2726
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 25 of 188 PageID #: 13517
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`53
`
`Q· · · ·And I'm just looking for, what do you
`
`consider to be similar performance?
`
`A· · · ·I don't know that I have a number.· I would
`
`say similar performance.· At least 20 percent -- if
`
`one achieved at least 20 percent, I wouldn't think
`
`the other would be different.
`
`Q· · · ·Do you think that they would achieve the
`
`same ligation efficiency?
`
`A· · · ·Similar ligation efficiencies.
`
`Q· · · ·How do you -- how do you characterize
`
`similar ligation efficiencies?
`
`A· · · ·They're similar.· So as I stated here, these
`
`experimental conditions are similar.·
`
` I
`
`don't have an exact number for you, but 20 percent
`
`was standard, so -- and I don't expect a big
`
`difference based on the experimental details given
`
`here.
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 53
`
`YVer1f
`
`A2727
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 26 of 188 PageID #: 13518
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`54
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 54
`
`YVer1f
`
`A2728
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 27 of 188 PageID #: 13519
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`55
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 55
`
`YVer1f
`
`A2729
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 28 of 188 PageID #: 13520
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`56
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 56
`
`YVer1f
`
`A2730
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 29 of 188 PageID #: 13521
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`57
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 57
`
`YVer1f
`
`A2731
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 30 of 188 PageID #: 13522
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`58
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 58
`
`YVer1f
`
`A2732
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 31 of 188 PageID #: 13523
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`63
`
`it's reproducible.
`
`Q· · · ·And it would be easy for somebody --
`
`assuming that information was available, as a person
`
`of -- as -- do you believe it would be easy to
`
`con- -- conduct those experiments?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· I guess I don't know what
`
`you mean by easy, but I think that the experiments
`
`should be reproducible given the exact components.
`
`BY MR. CONSTANT:
`
`Q· · · ·Do you know of any significant barriers for
`
`you as a practitioner to conduct those experiments?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· I would have to know the
`
`components of the experiment.· But if I did, I
`
`cannot think of a barrier that would prevent me from
`
`doing the experiment.
`
`BY MR. CONSTANT:
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 63
`
`YVer1f
`
`A2733
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 32 of 188 PageID #: 13524
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`67
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 67
`
`YVer1f
`
`A2734
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 33 of 188 PageID #: 13525
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`68
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`BY MR. CONSTANT:
`
`Q· · · ·And you don't know anyone else who has
`
`actually performed this Schmitt example method,
`
`correct?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· Sitting here right now,
`
`no.
`
`BY MR. CONSTANT:
`
`Q· · · ·Do you know -- and sitting here today, you
`
`could not tell me what the ligation efficiency of
`
`the Schmitt example method is --
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·MR. CONSTANT:· Let me strike that.
`
`BY MR. CONSTANT:
`
`Q· · · ·Sitting here today, you could not tell me
`
`what the resulting ligation efficiency would be from
`
`performing the Schmitt example method, correct?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· It's my opinion
`
` and given
`
`my expectation of what Schmitt would have done, that
`
`the ligation efficiency achieved would be more than
`
`20 percent.
`
`www.LexitasLegal.com/Premier
`www.LexitasLegal.com/Premier
`
`Lexitas
`Lexitas
`
`888-267-1200 Page 68
`
`YVer1f
`
`A2735
`
`
`
`Case 1:20-cv-01580-LPS Document 74-1 Filed 05/24/21 Page 34 of 188 PageID #: 13526
`
`GUARDANT HEALTH vsGUARDANT HEALTH vs
`
`Stacey Gabriel, PH.D.Stacey Gabriel, PH.D.
`
`FOUNDATION MEDICINEFOUNDATION MEDICINE
`March 11, 2021
`69
`
`·1
`
`·2
`
`·3
`
`·4
`
`·5
`
`·6
`
`·7
`
`·8
`
`·9
`
`10
`
`11
`
`12
`
`13
`
`14
`
`15
`
`16
`
`17
`
`18
`
`19
`
`20
`
`21
`
`22
`
`23
`
`24
`
`25
`
`BY MR. CONSTANT:
`
`Q· · · ·I'm not asking for more or less than
`
`20 percent.
`
`· · · · I'm just saying, sitting here today, can you
`
`tell me what the ligation efficiency would be --
`
`what the exact ligation efficiency would result from
`
`performing the Schmitt example method?· Correct?
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· I can't tell you the
`
`exact number.· I can tell you that it's my opinion
`
`that it would be at least 20 percent.
`
`BY MR. CONSTANT:
`
`Q· · · ·That it likely would be at least 20 percent,
`
`or do you know for sure that it would be over
`
`20 percent?
`
`A· · · ·I feel confident that it would be over
`
`20 percent.
`
`· · · · · · ·MR. MARANDETT:· Objection.
`
`· · · · · · ·THE WITNESS:· It's based on a number of
`
`other references that I point out in this
`
`declaration, a textbook, for example, that says that
`
`20 percent is a standard ligation efficiency to
`
`achieve, as well as other referenc
Accessing this document will incur an additional charge of $.
After purchase, you can access this document again without charge.
Accept $ Charge
Still Working On It
This document is taking longer than usual to download. This can happen if we need to contact the court directly to obtain the document and their servers are running slowly.
Give it another minute or two to complete, and then try the refresh button.
A few More Minutes ... Still Working
It can take up to 5 minutes for us to download a document if the court servers are running slowly.
Thank you for your continued patience.
This document could not be displayed.
We could not find this document within its docket. Please go back to the docket page and check the link. If that does not work, go back to the docket and refresh it to pull the newest information.
Your account does not support viewing this document.
You need a Paid Account to view this document. Click here to change your account type.
Your account does not support viewing this document.
Set your membership
status to view this document.
With a Docket Alarm membership, you'll
get a whole lot more, including:
- Up-to-date information for this case.
- Email alerts whenever there is an update.
- Full text search for other cases.
- Get email alerts whenever a new case matches your search.
One Moment Please
The filing “” is large (MB) and is being downloaded.
Please refresh this page in a few minutes to see if the filing has been downloaded. The filing will also be emailed to you when the download completes.
Your document is on its way!
If you do not receive the document in five minutes, contact support at support@docketalarm.com.
Sealed Document
We are unable to display this document, it may be under a court ordered seal.
If you have proper credentials to access the file, you may proceed directly to the court's system using your government issued username and password.
Access Government Site
