Case 1:20-cv-01580-LPS Document 1-5 Filed 11/23/20 Page 1 of 55 PageID #: 270
`Case 1:20-cv-01580-LPS Document 1-5 Filed 11/23/20 Page 1 of 55 PageID #: 270
`
`
`
`
`
`EXHIBIT 5
`
`EXHIBIT 5
`
`

`

`( 12 ) United States Patent
`Eltoukhy et al .
`
`US 10,801,063 B2
`( 10 ) Patent No .:
`( 45 ) Date of Patent :
`* Oct . 13 , 2020
`
`US010801063B2
`
`( * ) Notice :
`
`( 63 )
`
`( 54 ) METHODS AND SYSTEMS FOR DETECTING
`GENETIC VARIANTS
`( 71 ) Applicant : GUARDANT HEALTH , INC . ,
`Redwood City , CA ( US )
`( 72 ) Inventors : Helmy Eltoukhy , Atherton , CA ( US ) ;
`Amir Ali Talasaz , Atherton , CA ( US ) ;
`Stefanie Ann Ward Mortimer , Morgan
`Hill , CA ( US )
`( 73 ) Assignee : GUARDANT HEALTH , INC . ,
`Redwood City , CA ( US )
`Subject to any disclaimer , the term of this
`patent is extended or adjusted under 35
`U.S.C. 154 ( b ) by 0 days .
`This patent is subject to a terminal dis
`claimer .
`( 21 ) Appl . No .: 16 / 601,168
`( 22 ) Filed :
`Oct. 14 , 2019
`( 65 )
`Prior Publication Data
`Apr. 23 , 2020
`US 2020/0123602 A1
`Related U.S. Application Data
`Continuation of application No. 15 / 892,178 , filed on
`Feb. 8 , 2018 , which is a continuation of application
`No. 14 / 861,989 , filed on Sep. 22 , 2015 , now Pat . No.
`9,920,366 , which is a continuation of application No.
`PCT / US2014 / 072383 , filed on Dec. 24 , 2014 .
`( 60 ) Provisional application No. 61 / 948,509 , filed on Mar.
`5 , 2014 , provisional application No. 61 / 921,456 , filed
`on Dec. 28 , 2013 .
`Int . Cl .
`C12P 19/34
`C12Q 1/6869
`C12Q 1/6886
`G16B 15/00
`U.S. Cl .
`CPC
`
`( 51 )
`
`( 52 )
`
`( 58 )
`
`( 56 )
`
`Case 1:20-cv-01580-LPS Document 1-5 Filed 11/23/20 Page 2 of 55 PageID #: 271
`
`( 2006.01 )
`( 2018.01 )
`( 2018.01 )
`( 2019.01 )
`C12Q 1/6869 ( 2013.01 ) ; C12Q 1/6886
`( 2013.01 ) ; C12Q 2535/122 ( 2013.01 ) ; C12Q
`2600/158 ( 2013.01 ) ; G16B 15/00 ( 2019.02 )
`Field of Classification Search
`None
`See application file for complete search history .
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`CN
`EP
`
`OTHER PUBLICATIONS
`Daser , et al . “ Interrogation of genomes by molecular copy - number
`counting ( MCC ) . ” Nature Methods , 3 ( 6 ) : 447-453 ( 2006 ) .
`( Continued )
`
`Primary Examiner Kenneth R Horlick
`( 74 ) Attorney , Agent , or Firm Timothy Hott
`
`( 57 )
`ABSTRACT
`Disclosed herein in are methods and systems for determin
`ing genetic variants ( e.g. , copy number variation ) in a
`polynucleotide sample . A method for determining copy
`number variations includes tagging double - stranded poly
`nucleotides with duplex tags , sequencing polynucleotides
`from the sample and estimating total number of polynucle
`otides mapping to selected genetic loci . The estimate of total
`number of polynucleotides can involve estimating the num
`ber of double - stranded polynucleotides in the original
`sample for which no sequence reads are generated . This
`number can be generated using the number of polynucle
`otides for which reads for both complementary strands are
`detected and reads for which only one of the two comple
`mentary strands is detected .
`
`28 Claims , 11 Drawing Sheets
`
`

`

`US 10,801,063 B2
`Page 2
`
`( 56 )
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`GOIN 21/6428
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`
`

`

`US 10,801,063 B2
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