Case 1:12-cv-00274-LPS Document 74-3 Filed 05/06/13 Page 1 of 39 PageID #: 865
`Case 1:12—cv—OO274—LPS Document 74-3 Filed 05/06/13 Page 1 of 39 Page|D #: 865
`
`EXHIBIT C
`
`

`
`Case 1:12-cv-00274-LPS Document 74-3 Filed 05/06/13 Page 2 of 39 PageID #: 866
`“'1llflllillllflilllflllfilllllliififlllilllfllmlllllflfllfillflllllflfifi
`
`US008097405B1
`
`(12) Ulllted States Patent
`Engelhardt et al.
`
`(10) Patent No.:
`(45) Date of Patent:
`
`US 8,097,405 B1
`Jan. 17, 2012
`
`1/1932 Cféa ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~ 535/27
`4,310,552 A
`fihnargllllif 8‘ 31 ~~~~~~~~~~~~~ ~~ 424/180
`A
`4:358:35 A ,1, 11/1982 Falkow et 31.
`.................. N 435/5
`4,373,071 A
`2/1983 Itakura
`4,375,401 A
`3/1933 CatS1IT1P001aS
`2
`ij_’1:‘11]1I‘:e”‘1' """""""" " 536/29
`4:415:732 A
`11/1983 Camthers
`4,443,594 A
`4/1984 Buckmarm .................... ,, 536/27
`4,450,772 A
`7/ 1934 13eI10Vi0 61 31 ~~~~~~~~~~~~~ ~~ 514/311
`2522:1222:
`11;13::311::n
`2
`Sfitglhers
`4,534,647 A
`8/1985 Gross
`.............. .. 435/6
`4,581,333 A *
`4/1986 K01_1ri1s1<y et al.
`/
`455985049 A
`7;1986 Z"_hnkfi_
`1
`j,‘;22§f§§ Q
`@133? $331,; °”" """"""""" 536 27
`4,668,777 A
`5/1987 Caruthers
`4,707,440 A
`11/1987 Stavrianopoulos ............. .. 435/6
`
`.. 435/91
`4,708,935 A
`11/1987 Suhadolnik et al.
`.................... .. 536/29
`4,711,955 A * 12/1987 Ward et al.
`4,757,141 A
`7/1988 Fung
`4,828,979 A
`5/1989 K1
`1.
`..
`435/6
`
`4,333,251 A
`5/1939 Mjzgfttfl. ................ H 543/303
`4,347,240 A
`7/1939 Ryser
`(Continued)
`
`FOREIGN PATENT DOCUMENTS
`
`DE
`
`A1814134
`
`9/ 1970
`(Continued)
`
`OTHER PUBLICATIONS
`Hartman et a1., Biopolymers, vol. 20, No. 12, pp. 2635-2648, 1981 .*
`Biochemistry by Lehniger [Published by Worth Publishers, Inc., 70
`Fifth Avenue, New York, NY 10011] pp. 638-639, 1970.*
`Dunn et al., Cell vol. 12, pp. 23-26, 1977.*
`Becker et a1., “Irreversible Inhibition of Biotin Transport in Yeast by
`Biotinyl-p-nitrophenyl Ester,” Proc. Nat’l. Acad. Sci.
`(USA)
`5332504-2507 (1971)
`
`.
`(Continued)
`
`.
`.
`.
`Primary Examiner — John Brusca
`AUO}"I’l€y, Agent, 0}’ Firm —Natal1e Bogdanos,
`E11eGe“d1°fi3 E59
`
`ABSTRACT
`(57)
`A process for determining the sequence of nucleic acids of
`interest employs nucleotides or nucleotide analogs that have
`been made detectable by non-radioactive modifying or label-
`ing. Such nucleotides or nucleotide analogs are modified on
`the sugar moieties, the phosphate moieties or the base moi-
`eties, including base analogs. Modified nucleotide analogs
`can be attached to or coupled to or incorporated into DNA or
`RNA. The modified or labeled nucleotides or nucleotide ana-
`
`logs are also useful in processes for detecting the presence of
`nucleic acids of interest and for characterizing chromosomal
`sequences. Detection processes using the modified or labeled
`nucleotides or nucleotide analogs extend to the use ofa gel for
`separating or resolving hybrids formed between non-radio-
`actively labeled oligonucleotides orpolynucleotides and such
`nucleic acids of interest.
`
`198 Claims, 4 Drawing Sheets
`
`(54) NUCLEIC ACID SEQUENCING PROCESSES
`USING NON-RADIOACTIVE DETECTABLE
`MODIFIED OR LABELED NUCLEOTIDES OR
`NUCLEOTIDE ANALOGS, AND OTHER
`PROCESSES FOR NUCLEIC ACID
`DETECTION AND CHROMOSOMAL
`CHARACTERIZATION USING SUCH
`NON-RADIOACTIVE DETECTABLE
`MODIFIED OR LABELED NUCLEOTIDES OR
`
`(75)
`
`Inventors: Dean Engelhardt, New York, NY (US);
`Elazar Rabbani, New York, NY (US);
`Stanley Kline, Brooklyn, NY (US);
`Jannis G. Stavrianopoulos, New York,
`NY (Us); Dollie Kirtikar, Elmhurst, NY
`(US)
`
`(73) Assignee: Enzo Biochem, Inc., Farrningdale, NY
`(US)
`.
`.
`.
`.
`Sublect. 10 any dlsclalmers. the term Ofthls
`patent 1s extended or adjusted under 35
`U.S.C. 154(b) by 0 days.
`
`.
`( * ) N01199:
`
`(21) APP1 N0‘ 08/489069
`
`(22)
`
`Filed:
`
`Jun. 7, 1995
`
`Related U.S. Application Data
`
`(60) Continuation of application No. 07/954,772, filed on
`S613, 30, 1992, now abandoned, which is a continuation
`Of aPP11Ca110I1 N0, 07/548,348, 1'11€d
`011 -1111, 2, 1990,
`now abandoned, which is a division of application No.
`07/140,980, filed on Jan. 5, 1988, now abandoned,
`which is a Continuation ofapplication No. 06/674,352,
`filed on Nov, 21, 1984, now abandoned, which is a
`continuation of application No. 06/391,440, filed on
`Jun. 23, 1982, now abandoned.
`
`(51)
`
`Int. Cl.
`C12Q 1/68
`
`(2006.01)
`
`......................................... .. 435/6; 536/243
`(52) U.S. Cl.
`(58) Field of Classification Search ................ .. 435/5, 6,
`435/7.1, 91.1, 91.2, 810; 436/501, 63; 536/22.1,
`536/231, 24.1, 243—2433, 25.3; 735/77,
`735/78
`See application file for complete search history.
`
`(56)
`
`References Clted
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`
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`
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`
`
`
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`
`

`
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`US 8,097,405 B1
`Page 2
`
`U.S. PATENT DOCUMENTS
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`........... .. 536/27
`
`........ .. 536/23.1
`
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`...................... .. 536/27
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